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OD ODã¨ã¯ã€Opitical Densitiy(å…‰å¦å¯†åº¦)ã®ç•¥ã§ã€ä¸€èˆ¬çš„ã«ã¯å¸å…‰åº¦ã¨å‘¼ã°ã‚Œã‚‹ã€‚ä¸€èˆ¬çš„ã«ã€åˆ†å…‰å…‰åº¦è¨ˆã¯å¸å…‰åº¦0.100ã€œ1.000ã®ç¯„å›²ã§ã‚‚ã£ã¨ã‚‚ç²¾åº¦è‰¯ãæ¸¬å®šã™ã‚‹ã“ã¨ãŒã§ãã¾ã™ã€‚å¸å…‰åº¦ã¯è‹±èªžã§"absorbance"ã§ã‚ã‚‹ã®ã§ã€"A"ã‚„"Abs"ã¨ç•¥è¨˜ã•ã‚Œã‚‹ã€‚ä¾‹ãˆã°A260ã¨ã¯ã€"(å¯¾è±¡ã®æº¶æ¶²ã®)æ³¢é•·260nmã«ãŠã‘ã‚‹å¸å…‰åº¦"ã‚’æ„å‘³ã™ã‚‹ã€‚ ãƒ©ãƒ³ãƒ™ãƒ«ãƒˆãƒ»ãƒ™ãƒ¼ãƒ«ã®æ³•å‰‡ã€€Lambert-Beer law å¸å…‰åº¦ã¯ã€Žæ¿ƒåº¦ï½ƒãŒä¸€å®šãªã‚‰ã€é€éŽå…‰ã®å¼·ã• Iã¯ã€é€éŽã™ã‚‹å±¤ã®åŽšã•LãŒåŽšããªã‚‹ã¨ã€æŒ‡æ•°é–¢æ•°çš„ã«å°‘ãªããªã‚‹(ãƒ©ãƒ³ãƒ™ãƒ«ãƒˆã®æ³•å‰‡)ã€‚ã¾ãŸã€é€éŽã™ã‚‹å±¤ã®åŽšã•LãŒä¸€å®šãªã‚‰ã€æ¿ƒåº¦ï½ƒãŒæ¿ƒããªã‚‹ã¨é€éŽå…‰ã®å¼·ã•Iã¯æŒ‡æ•°é–¢æ•°çš„ã«æ¸›å°‘ã™ã‚‹(ãƒ™ãƒ¼ãƒ«ã®æ³•å‰‡)ã€‚ã€ã¨ã„ã†ãƒ©ãƒ³ãƒ™ãƒ«ãƒˆãƒ»ãƒ™ãƒ¼ãƒ«ã®æ³•å‰‡ã§è¦å®šã•ã‚Œã‚‹ã€‚ å…¥å°„å…‰ã®å¼·ã•ã‚’ I0ã€é€éŽå…‰ã®å¼·ã•ã‚’ Iã€å¸å…‰åº¦ã‚’ Aã€å…‰ãŒé€šéŽã™ã‚‹å±¤ã®åŽšã•ã‚’ Lã€å…‰ã®å¸åŽã‚’ã™ã‚‹ç‰©
asrite 2009/09/29
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