BASIC PHYSICAL CHEMISTRY 3.

2 Rate of decay, Half-life, Units of radioactivity, radioactive dating,

EC502 law of successive disintegration, radioactive equilibrium, age
determination - dating by C14.
Lecture : 3 Year:II 3.3 Nuclear binding energy, Fission & fusion, Nuclear chain reaction,
Tutorial : 1 Part: I Nuclear energy
Practical : 3
4. Theory of dilute Solution (3 hours)
Course Objective: 4.1 Colligative properties, lowering of vapor pressure (Raoults law).
To familiarize the student with basic principle of physical chemistry which Elevation of boiling point, depression of freezing point, osmotic
can be applied in chemical engineering. pressure.
4.2 Colligative properties of electrolyte.

1. Atomic Structure (wave mechanical concept) (5 hours) 5. Principle of Titration (4 hours)

1.1 de Broglie equation, Group and phase velocity, Heisenberg’s 5.1 Volumetric titration
uncertainty principle and its applications 5.2 Conductivity of electrolyte, solubility products of sparingly
1.2 Wave function and its significance, Schrodinger wave equation; soluble salts conductometric titration
Quantum tunneling, particle in 1D box 5.3 pH and pH scale, determination of pH using glass, quinhydrone
1.3 Quantum numbers and ground state electron configuration of and antimony-antimony oxide electrodes, Potentiometric titration
elements
6. Colloids (3 hours)
2. Thermodynamics (5 hours) 5.1 Lyophilic and lyophobic sols or colloids, dialysis, Tyndall effect
2.1 Adiabatic expansion of an ideal gas (TV-relation, PV-relation and 5.2 Electrophoresis, gold number
PT relation), comparison between isothermal and adiabatic 5.3 Cleansing action of detergents, emulsion, gel, determination of
expansion, work done in reversible adiabatic expansion, Joule‟s molecular weight of macromolecules.
Thomson effect.
7. Chemical Kinetics (5 hours)
2.2 Second law of thermodynamics, Heat Engine and Efficiency,
7.1 Rate of reaction, order of reaction, integrated rate equation for
Reversible and Irreversible process, Internal combustion Engine,
Entropy and its physical significance. zero, first, second and third order reaction
7.2 Rate constant, half-life period, measurement of order of reaction,
[2.3] Free energy and work function, criteria of spontaneity and
equilibrium in terms of entropy and free energy, study of kinetic of two reactions (hydrolysis & saponification)
7.3 Factors affecting rate of reaction, Arrhenius equation
2.3[2.4] Third law of thermodynamic, Gibbs Helmholtz equation and
Clapeyron-Calusius equation 8. Physical Properties & chemical constitution (8 hours)
3. Nuclear chemistry (3 hours) 8.1 Surface tension and chemical constitution, viscosity and
3.1 Types of radiation, Fajans-Soddy displacement law, Disintegration
chemical constitution
8.2 Rheochor, parachor and dipole moment
series
8.3 Optical activity and chemical constitution, Magnetic properties
(para and dia)
8.4 Electromagnetic spectrum, molecular energy levels 7. To determine water equivalent and the heat of neutralization of strong acid
8.5 Rotational, vibrational and electronic energy (HCl) and strong base (NaOH) (6 hours)
8.6 IR, UV-Visible, Raman, NMR, Mass spectroscopy
Evaluation Scheme:
9. Photochemistry and electrocatalysis (4 hours) The questions will cover all the chapters in the syllabus. The evaluation
9.1 Photochemical reaction, laws of photochemistry scheme will be as indicated in the table below. There can be slight
9.2 Quantum yield, photosynthesized reactions, electrocatalyst variation in the questions according to the respective tutor.
9.3 Photo physical process, fluorescence, phosphorescence,
chemiluminescence Unit Chapter Topics Marks
10. Surface chemistry (3 hours) 1 1&2 all 16
10.1 Adsorption; types and mechanism 2 3, 4 & 5 all 16
10.2 Adsorption isotherms; Freundlich and Langmuir 3 6&7 all 16
10.3 Application of adsorption
4 8 all 16
10.4 Ion-exchange adsorption and its application
5 9, 10 & 11 all 16
11. Separation methods (2 hours)
Total 80
11.1 Solvent extraction
11.2 Chromatography (TLC, column, GC-MS, HPLC)

References:
1. Maron & Prutton, “Principles of Physical Chemistry, The Macmillan
Company
2. Arun Bahl, B. S. Bahl & G.D. Tuli “Essential of Physical Chemistry”, S.
Chand Publication, New Delhi
Practical:
1. To study the kinetics of the oxidation of iodides by persulphate solution
using iodine clock method (3 hours)
2. To find out the strength of ammonia solution by titrating against acetic
acid solution using pH meter (3 hours)
3. To determine the critical micelle concentration of soap (3 hours)
4. To calculate the molecular weight of high polymer by means of viscosity
measurement (3 hours)
5. To determine the concentration of acetic acid by titration with ammonium
hydroxide conductometrically (3 hours)
6. To prepare the salt bridge and to determine the concentration of Cl - ion in
KCl solution by potentiometric titration (6 hours)
BIOCHEMISTRY 4.1 Principle and applications of centrifugation techniques, gel filtration
EC501 chromatography, gel electrophoresis,
Lecture : 3 Year : II 4.2 Gene transfer methods: electroporation and particle gun.
Tutorial : 1 Part : I 4.3 X-ray diffraction techniques and MALDI-TOF technique.
5. Enzymes: (10
Course Objective: hours)
To familiarize the student with basic principle of biochemistry which can be 5.1 Introduction, classifications and reactions, structure of enzymes.
applied in chemical engineering. 5.2 Isolation, purification and functions of enzymes, enzyme assay and
activity.
1. Biomolecules (7 hours)
5.3 Coenzymes and cofactors, isoenzymes, pro-enzymes, multienzyme
1.1 Structure, classification, physical and chemical properties of complexes and tandom enzymes, 5.4. Regulation of enzyme activity:
carbohydrates, proteins, amino acid, lipids, and Vitamins in effects of pH, substrate, enzyme concentration, temperature, cofactor
biochemical aspects. and additives.
1.2 Structure and function of purine and pyrimidine nucleotides, codons, 5.5 Mechanism of enzyme action, enzyme specificity, active sites,
sequencing and organization of genome, mutation. covalent modification.
1.3 Structural composition of deoxyribonucleic acid (DNA), Biosynthesis 5.6 Kinetics of catalyzed reaction, Michaelis-Menten equation and its
of DNA (replication) and protein (translation). limitation, Briggs Haldane relationship.
1.4 Type of ribonucleic acids (RNA) and structures, biosynthesis of RNA 5.7 Inhibition of enzymes, immobilized enzyme and microbial cell,
(transcription). applications of enzymes in clinical, foods, agriculture and
2. Microbial Biochemistry (2 environment.
hours) 6. Metabolism: Concept of metabolism (7
2.1 Structural composition of gram positive and gram negative bacteria, hours)
structures and characteristics of bacterial protein toxin. 6.1 Carbohydrate Metabolism: Carbohydrates source, glycolysis, HPM
2.2 Viruses and their life cycles (lytic and lysogeny). shunts, TCA cycle, glycogenolysis, glycogen synthesis, glyoxylate
3. Molecular genetics (4 cycle.
hours) 6.2 Amino acid Metabolism: nutritional and metabolic importance of
amino acids, catabolism of amino acids, anabolic metabolism of few
3.1 Gene, primer, Polymerase chain reaction (PCR). individual amino acids, urea cycle and metabolic diseases.
3.2 Gene cloning, plasmid and recombinant plasmid, process of gene 6.3 Lipid Metabolism: Concept of lipid metabolism, nutritional
expression, protein preparation and its purification, importance, biosynthesis and degradation of simple and complex
3.3. Introduction of transgenic animals and plants, scopes of molecular lipids, metabolism regulation, abnormalities in lipid metabolism.
genetics.
7. Metabolites: (3 hours)
4. Biochemical Techniques:
(3hours) 7.1 Definition of metabolites, type of metabolites, production of
metabolites in batch and continuous fermentation.
7.2 Antibiotics, sources of antibiotics, mechanism of action of antibiotics, 6. Biotechnology of Antibiotics and Other Bioactive Microbial Metabolites,
hybrid antibiotics, DNA antibiotics interaction. 1993 rd Edition by Lancini G. and Lorenzetti R.
7. Introduction to Biofuels, 1st Edition by Mousdale D. M.

8. Agricultural biochemistry (4
hours)
8.1 Introduction of plant growth promoting rizobacteria (PGPR), effects
of PGPR on plant physiology and growth, induced systemic Evaluation Scheme:
resistance, biocontrol of plant pathogens, bio-fertilization.
The questions will cover all the chapters in the syllabus. The evaluation
8.2 Sources and chemical structures of some PGPR-based metabolites
scheme will be as indicated in the table below. There can be slight
such as antibiotics, volatile organic compounds, hydrolytic enzymes,
variation in the questions according to the respective tutor.
siderophores, surfactins and their agricultural applications.
9. Biofuel & biochemicals: (5
Unit Chapter Topics Marks
hours)
1 1&2 all 16
9.1 Concept of biofuels and biochemicals, different between biofuels and 2 16
3&4 all
petroleum-based gasoline, biomass and their conversion into biofuels
3 5 all 16
and biochemicals.
9.2 Study about the biosynthetic pathway of some important biofuels such 4 6&7 all 16
as bioethanol, 2, 3-butanediol and biochemical lactic acid. 5 8&9 all 16
Total 80
Reference Books
1. Gene cloning and DNA analysis, An Introduction, 6th Edition by
BROWN T.A.
2. Lehninger Principle of Biochemistry, 4th edition by Nelson D. L. and
Michel M. Cox.
3. Plant Growth Promoting Rhizobacteria (PGPR): Prospects for Sustainable
Agriculture by Sayyed R. Z, Reddy M.S. and Sarjiya A.
4. Fundamentals of Biochemistry: Life at the Molecular Level by Donald V.
and Judith G. V.
5. Secondary metabolites, 2nd Edition by Ramawat K.G and Merillon J.M.