Intel ISEF Rules and Guidelines 2020 2021

INTERNATIONAL RULES

FOR PRE-COLLEGE SCIENCE

RESEARCH
GUIDELINES FOR SCIENCE AND ENGINEERING FAIRS 2020–
2021
INTERNATIONAL RULES FOR PRE-COLLEGE SCIENCE
RESEARCH: GUIDELINES FOR SCIENCE AND ENGINEERING
FAIRS 2020–2021
TABLE OF CONTENTS
General Requirements for All Projects
1. ISEF Ethics Statement................................................................................................................................................................................................ 3
2. ISEF Eligibility/Limitations....................................................................................................................................................................................... 3
3. ISEF Requirements.................................................................................................................................................................................................... 3
4. Continuation/Research Progression of Projects.......................................................................................................................................................... 4
5. Team Projects............................................................................................................................................................................................................. 4
Roles and Responsibilities of Students and Adults

6. The Student Researcher(s).......................................................................................................................................................................................... 5
7. The Adult Sponsor..................................................................................................................................................................................................... 5
8. The Qualified Scientist............................................................................................................................................................................................... 5
9. The Designated Supervisor......................................................................................................................................................................................... 5
10. The Institutional Review Board (IRB)........................................................................................................................................................................ 6
11. The Affiliated Fair Scientific Review Committees (SRC)..........................................................................................................................................6
12. Regulated Research Institution/Industrial Settings Review Committees....................................................................................................................7
13. ISEF Scientific Review Committee............................................................................................................................................................................ 7
Human Participants Rules

1. Rules.......................................................................................................................................................................................................................... 8
2. Exempt Studies.......................................................................................................................................................................................................... 9
3. Human Participant & IRB Resources.......................................................................................................................................................................11
Vertebrate Animals Rules

1. Rules for ALL Vertebrate Animal Studies...............................................................................................................................................................12
2. Additional Rules for Projects Conducted at School/Home/Field..............................................................................................................................13
3. Additional Rules for Projects Conducted in a Regulated Research Institution.........................................................................................................14
Potentially Hazardous Biological Agents Rules

1. Rules for ALL Projects Involving Potentially Hazardous Biological Agents...........................................................................................................15
2. Additional Rules for Projects Involving Unknown Microorganisms........................................................................................................................16
3. Additional Rules for Projects Involving Recombinant DNA (rDNA) Technologies............................................................................................................16
4. Additional Rules for Projects Involving Tissues and Body Fluids Including Blood and Blood Products.............................................................................17
5. Potentially Hazardous Biological Agents Risk Assessment......................................................................................................................................18
Hazardous Chemicals, Activities or Devices Rules

1. Rules for ALL Projects Involving Hazardous Chemicals, Activities or Devices......................................................................................................19
2. Additional Rules for Specific Regulated Areas....................................................................................................................................................... 19
3. Hazardous Chemicals, Activities or Devices Risk Assessment................................................................................................................................21
Engineering & Inventions Projects Guide................................................................................................................................................22
Sources of Information...............................................................................................................................................................................23
Display & Safety Regulations.....................................................................................................................................................................26
Categories & Sub-Categories.....................................................................................................................................................................29
Information on Required Abstract............................................................................................................................................................30
Forms............................................................................................................................................................................................................31
The International Rules and Guidelines for Science Fairs is available at societyforscience.org/ISEF20201 in multiple formats. Familiarity with the rules is
critical for students, parents, teachers, mentors, fair directors and local and affiliated fair Scientific Review Committees (SRC) and Institutional Review Boards
(IRB).
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International Rules: Guidelines for Science and Engineering Fairs 2020–2021,
societyforscience.org/ISEF2021
INTERNATIONAL RULES AND GUIDELINES
The full text of the International Rules and forms in html and as a
downloadable pdf.
ISEF RULES WIZARD

An interactive tool which asks questions about your intended project and provides
a list of forms required.
COMMON SRC PROBLEMS

Frequent problems that emerge during Scientific Review Committee review for
qualification at ISEF. Read these to learn what NOT to do.
THESE RULES ARE APPLICABLE FOR

Society for Science & the Public affiliated science fairs and the
Regeneron International Science & Engineering Fair 2021.
The purpose of these rules is to:
• protect the rights and welfare of the student researcher
• protect the rights and welfare of human participants
• protect the health and welfare of vertebrate animal subjects
• protect and promote good stewardship of the environment
• ensure adherence to federal regulations
• ensure use of safe laboratory practices
• determine eligibility for competition in ISEF
For pre-review and approval of your project, find your fair at:
https://findafair.societyforscience.org/
For rules questions, contact the ISEF Scientific Review Committee:
[email protected]
For general questions, contact: Society for
Science & the Public Science
Education Programs
1719 N Street, NW, Washington, DC 20036 office:
202-785-2255, fax: 202-785-1243
email: [email protected]

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ALL PROJECTS
Ethics Statement
Eligibility/Limitations
Student researchers, as well as adults who have a role in their projects, are
expected to maintain the highest ethical standards. These standards include, 1. Each ISEF-affiliated fair may send to ISEF the number of projects
but are not limited to: allocated and committed to within their affiliation agreement.
• Integrity. Honesty, objectivity, and avoidance of conflicts of interest are 2. A student must be selected by an ISEF-affiliated fair, and meet both of the
expected during every phase of the project. The project should reflect following:
independent research done by the student(s) and be free of fraudulent data a. be in grades 9–12 or equivalent; and
and/or plagiarism and represent only one year’s work. b. not have reached age 20 on or before May 1 preceding ISEF.
• Legality. Compliance with all federal, state and local laws and
regulations is essential. In addition, projects conducted outside the U.S. 3. English is the official language of ISEF. Student project boards and
must also adhere to the laws of the country and jurisdiction in which the abstracts must be in English.
project was performed. All projects must be approved by a Scientific
Review Committee (SRC), and when necessary must also be approved 4. Each student is only allowed to enter one project. That project may include
by an Institutional Review Board (IRB), Institutional Animal Care and no more than 12 months of continuous research and may not include
Use Committee (IACUC), and/or Institutional Biosafety Committee research performed before January 2020. NOTE: Projects that were
(IBC). Native, genetically-altered, and/or invasive species, (e.g. insects, conducted between January 2020 and March 2020 that competed at an
plants, invertebrates, vertebrates), pathogens, toxic chemicals or foreign ISEF-affiliate fair, may not be presented in 2021 without meeting the
substances reintroduced into the environment is prohibited. It is continuation criteria.
recommended that students reference their local, state or national laws
and regulations. 5. Team projects must have no more than three members. Teams competing
• Respect for Confidentiality and Intellectual Property. Confidential at ISEF must be composed of the original members who competed at the
communications, as well as patents, copyrights, and other forms of ISEF-affiliated fair and must all meet ISEF eligibility.
intellectual property must be honored. Unpublished data, methods, or
results may not be used without permission, and credit must be given for 6. Students may compete in only one ISEF affiliated fair, except when
all contributions to the research. proceeding to a state/national fair affiliated with ISEF from an affiliated
• Stewardship of the Environment. It is the responsibility of the regional fair.
researcher and the adults involved to protect the
environment from harm. Introduction or disposal of native, genetically- 7. Projects that are demonstrations, ‘library’ research or
altered, and/or invasive species, (e.g. insects, plants, invertebrates, informational projects, ‘explanation’ models are not
vertebrates), pathogens, toxic chemicals or foreign substances into the recommended or appropriate for ISEF.
environment is prohibited. It
is recommended that students reference their local, state or national 8. All sciences and engineering disciplines are represented at ISEF and
regulations and quarantine lists. projects compete in one of the 22 categories.
• Acknowledgment of Risks. All projects involve some amount of risk. Review a complete list of categories and sub-categories with definitions.
Everyone is expected to recognize the hazards, assess the risks, minimize
the risks, and prepare for emergencies. 9. A research project may be a part of a larger study performed by
• Animal Care. Proper care and respect must be given to vertebrate professional scientists, but the project presented by the student must be
animals. The use of non-animal research methods and alternatives to only their own portion of the complete study.
animal research are strongly encouraged and must be explored before
conducting a vertebrate animal project. The guiding principles for the
REQUIREMENTS
use of animals in research includes the following “Four R’s:’ Replace, General
Reduce, Refine, Respect. 1. All domestic and international students competing in an ISEF-affiliated
• Human Participant Protection. The highest priority is the health and fair must adhere to all rules as set forth in this document.
well-being of the student researcher(s) and human participants.
• Potentially Hazardous Biological Agents (PHBAs). It is the 2. All projects must adhere to all of the tenets of the Ethics
responsibility of the student and adults involved in the project to conduct Statement.
and document a risk assessment, and to safely handle and dispose of
organisms and materials. 3. It is the responsibility of the student and the Adult Sponsor to evaluate
the study to determine if the research will require forms and/or review
Scientific fraud and misconduct are not condoned at any level of research or and approval prior to experimentation.
competition. This includes plagiarism, forgery, use or presentation of other
researcher’s work as one’s own and fabrication of data. Fraudulent projects 4. Projects competing at ISEF must have an exhibit that adheres to ISEF
will fail to qualify for competition in affiliated fairs and ISEF. Society for Display & Safety requirements and is visible during
Science and the Public reserves the right to revoke recognition of a project all operable hours of the exhibit hall without reliance on electricity
subsequently found to have been fraudulent. or internet connections.
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5. All projects must adhere to the requirements of the affiliated fair(s) in
10. All signed forms, certifications, and permits must be available for review
which it competes to qualify for participation in ISEF. Affiliated fairs
by all regional, state, national and international affiliated fair SRCs in
may have additional restrictions or requirements. Knowledge of these
which the student(s) participate.
requirements is the responsibility of the student and Adult Sponsor.
This review must occur after experimentation and before
Approval and Documentation competition.
1. Project documentation should begin before experimentation with the
Digital Paperwork and Signatures
current forms available. Projects involving human participants, vertebrate
Submission of forms generated by a digital system are allowable under the
animals, and potentially hazardous biological agents must be reviewed and
following conditions:
approved by a local or regional Institutional Review Board (IRB) or
1. The forms must have the same content and order as ISEF forms.
Scientific Review Committee (SRC) prior to the start of experimentation.
urrent at the start of the project which may in some cases be prior
2. Digital signatures must have a verification system via login and have a
to experimentation begins, a local or regional Institutional Review Board
time and date stamp to indicate this authentication.
(IRB) or Scientific Review Committee (SRC) with the ISEF-affiliated
fair must review and approve most projects involving human participants,
3. Paperwork submitted to Society for Science & the Public for ISEF must
vertebrate animals, and potentially hazardous biological agents. Note: If a
be scanned and submitted via the online portal.
project involves the testing of a student designed invention, prototype
or concept by a human, an IRB review and approval may be required Continuation/Research Progression of Projects
prior to experimentation. See Human Participants Rules for details. 1. As in the professional world, research projects may build on work
performed previously. A valid continuation project is a sound scientific
2. Every student must complete the Student Checklist (1A), a Research endeavor. Students will be judged only on laboratory experiment/data
Plan/Project Summary and Approval Form (1B) and review the project collection performed over 12 continuous months beginning no earlier
with the Adult Sponsor in coordination with completion by the Adult than January 2020 and ending May 2021.
Sponsor of the Checklist for Adult Sponsor (1).
2. Any project based on the student’s prior research could be considered a
3. A Qualified Scientist is required for all studies involving Biosafety Level continuation/research progression project. These projects must document
2 (BSL-2) potentially hazardous biological agents and DEA-controlled that the additional research is a substantive expansion from prior work
substances and is also required for many human participant studies and (e.g. testing a new variable or new line of investigation). Repetition of
many vertebrate animal studies. previous experimentation with the same methodology and research
question, even with an increased sample size, is an example of an
4. After initial IRB/SRC approval (if required), any proposed changes unacceptable continuation.
in the Student Checklist (1A) and Research Plan/ Project Summary
must be re-approved before laboratory experimentation/data 3. The display board and abstract must reflect the current year’s work only.
collection resumes. The project title displayed in the finalist’s booth may mention years (for
example, “Year Two of an Ongoing Study”). Previous year’s databooks,
5. Projects which are continuations of a previous year’s work and which research papers and supporting documents may be at the booth if properly
require IRB/SRC approval must undergo the review process with the labeled as such.
current year ResearchPlan/Project Summary prior to experimentation/data
collection for the current year. 4. Longitudinal studies are permitted as an acceptable
continuation under the following conditions:
6. Any continuing project must document that the additional research is a. The study is a multi-year study testing or documenting the same
new and different. (Continuation/Research Progression Projects Form variables in which time is a critical variable. (Examples: Effect of
(7)). high rain or drought on soil in a given basin, return of flora and
fauna in a burned area over time.)
7. If work was conducted in a regulated research institution, industrial b. Each consecutive year must demonstrate time-based change.
setting or any work site other than home, school or field at any time c. The display board must be based on collective past
during the current ISEF project year, the Regulated Research conclusionary data and its comparison to the current year data
Institutional/Industrial Setting Form (1C) must be completed and set. No raw data from previous years may be displayed.
displayed at the project booth.
5. All projects must be reviewed and approved each year and forms must
8. After experimentation, each student or team must submit a (maximum) be completed for the new year.
250-word, one-page abstract which summarizes the current year’s work.
Team Projects
The abstract must describe research conducted by the student, not by the
1. Team projects compete and are judged in the category of their research at
supervising adult(s).
ISEF. All team members must meet the eligibility requirements for ISEF.
9. A project data book and research paper are not required, but are strongly
2. Teams must have no more than three members. A team with members from
recommended for judging purposes. Regional or local fairs may require a
different geographic regions may compete at an affiliated fair of one of its
project data book and/or a research paper.
members, but not at multiple fairs. However, each affiliated fair holds the
authority to determine

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whether teams with members outside of a fair’s geographic territory
be familiar with the regulations around procedures and materials that
are eligible to compete, understanding that if
apply to the student project, particularly when involving human
the team wins the right to attend ISEF, all team members’ expenses
participants, vertebrate animals, potentially hazardous biological agents
must be supported by the fair.
or hazardous chemicals, devices or activities.
a. Team membership cannot be changed during a given research year
• Should have close contact with the student throughout the timeline of
unless there are extenuating circumstances and the local SRC
the project.
reviews and approves the change, including converting a team
project to an individual project or vice versa. Such conversions must
Responsibilities:
address rationale for the change and include a clear delineation
The Adult Sponsor is responsible for:
between research preceding the change and that which will follow. A
• Working with the student to evaluate any possible risks involved in
memorandum documenting this review and approval should be
order to ensure the health and safety of the student conducting the
attached to Form 1A.
research and the humans and/or animals involved in the study.
b. Once a project has competed in a science fair at any level, team
• Reviewing the student’s Student Checklist (1A) and Research
membership cannot change and the project cannot be converted from
Plan/Project Summary to ensure that:
an individual project to a team project or vice versa.
• experimentation follows local, state, and Federal laws and ISEF
c. In a future research year, any project may be converted from an
rules
individual to a team project, from a team to
• forms are completed by other required adults
an individual project and/or have a change in team
• any required Qualified Scientist meets the criteria as set forth in
membership.
the ISEF Rules and Guidelines
• the student’s research is eligible for entry in ISEF
3. Each team is encouraged to appoint a team leader to coordinate the work
and act as spokesperson. However, each member of the team should be The Qualified Scientist (QS)
able to serve as spokesperson, be fully involved with the project, and be Qualifications:
familiar with all aspects of the project. The final work should reflect the • Earned a doctoral/professional degree in a scientific discipline related to
coordinated efforts of all team members and will be evaluated using the student’s area of research
same judging criteria as individual projects. AND/OR
• Individual with extensive experience and expertise in the student’s
4. Each team member must submit an Approval Form (1B). Team members area of research
must jointly submit the Checklist for Adult Sponsor (1), one abstract, a • Must be thoroughly familiar with the following regulations that govern the
Student Checklist (1A), a Research Plan/ Project Summary and other student’s area of research including all local, state, Federal and if
required forms. applicable, non-U.S. national regulations and laws.
• Can also serve as the Adult Sponsor, if that person meets both sets of
5. Full names of all team members must appear on the abstract and forms. qualifications
• May live elsewhere and not be local to the student, in which case, a
ROLES AND RESPONSIBILITIES Designated Supervisor has been appointed and trained to serve as the
OF STUDENTS AND ADULTS onsite supervision as necessary for the specific student project.
The Student Researcher(s) Responsibilities:

The student researcher is responsible for all aspects of the research project : The Qualified Scientist is responsible for:
• Enlisting the aid of any required supervisory adults (Adult Sponsor, • Reviewing the ISEF rules relevant to the project and approving the
Qualified Scientist, etc.), obtaining necessary approvals (SRC, student’s research plan or engineering design prior to the start of
IRB, etc.) experimentation
• Following the International Rules & Guidelines and obtaining all • Providing direct supervision throughout the timeline of the project or
necessary approvals (SRC, IRB, etc.) and completing all appropriate coordinating with a Designated Supervisor to serve in this capacity
documentation • Ensuring the proper training of the Student Researcher and/or Designated
• Performing the project (which may include, but is not limited to) Supervisor in the necessary procedures
experimentation, data collection, engineering, data analysis, and any • Completing the required documentation which may include the Regulated
other process or procedures related to the project Research Institutional Setting Form (1C), the Qualified Scientist Form (2)
• Understanding and abiding by the Ethics Statement and attesting to and the Risk Assessment Form (3), when applicable.
this understanding on Approval Form 1B.
The Designated Supervisor (DS)
To avoid conflict of interest, no Adult Sponsor, parent or other relative of the Qualifications:
student, the Qualified Scientist, or Designated Supervisor who oversees the • Does not need an advanced degree
project, may serve on the SRC or IRB reviewing that project. • Must be familiar with the student’s project and agree to any training
necessary
The Adult Sponsor • May also serve as the Adult Sponsor for the project
Qualifications: • If the project involves the use of Vertebrate Animals (where
• An Adult Sponsor may be a teacher, parent, professor, and/or other behavior/habitat is influenced by humans), must be
professional scientist knowledgeable about the humane care and handling of the animals
• Should be knowledgeable in the area of student research,
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Responsibilities:
animal research and research involving potentially hazardous biological
• Providing direct supervision of the student experimentation
agents and/or hazardous chemicals, activities or devices
• Completing the required documentation — the Designated Supervisor
• Compliance with ISEF ethics statement
box on the Qualified Scientist Form (2) when applicable
• Use of accepted and appropriate research techniques
• Reviewing and completing the Risk Assessment Form (3) when
• Evidence that risks have been properly assessed
needed
• Evidence of search for alternatives to animal use
REVIEW COMMITTEES • Humane treatment of animals
• Documentation of substantial expansion for continuation projects
To avoid conflict of interest, no Adult Sponsor, parent or other relative of • Evidence of appropriate literature search and attribution
the student, the Qualified Scientist, or Designated Supervisor who oversees
the project, may serve on the IRB reviewing that project or any other FOR HUMAN PARTICIPANT PROJECTS
committee. Additional members are recommended to help avoid a potential
conflict of interest and to increase the expertise of the committee.
REVIEW— THE INSTITUTIONAL REVIEW
BOARD (IRB)
Affiliated Fair Scientific Review Committee (SRC) An Institutional Review Board (IRB), is a committee that, according to federal
A Scientific Review Committee (SRC) is a group of qualified individuals that regulations (45-CFR-46), must evaluate the potential physical and/or
is responsible for evaluation of student research, certifications, research plans psychological risk of research involving humans. All proposed human research
and exhibits for compliance with the rules, applicable laws and regulations at must be reviewed and approved by an IRB before experimentation begins. This
each level of science fair competition. Affiliated Fairs may authorize local includes review of any surveys or questionnaires to be used in a project.
SRCs to serve in this prior review capacity. The operation and composition
of the local and Affiliated Fair SRCs must fully comply with the Federal regulations require local community involvement. Therefore, it is
International Rules. Directions for obtaining preapproval are available advisable that an IRB be established at the school level to evaluate human
from the affiliated fair. A list of fairs can be found at https://findafair. research projects. If necessary, the local or ISEF-affiliated SRC can serve as an
societyforscience.org. IRB as long as it has the required membership. An IRB must consist of a
minimum of three members including the following:
Most proposed research projects involving vertebrate animals and/or • An educator
potentially hazardous biological agents must be reviewed and approved • A school administrator (preferably principal or vice principal)
BEFORE experimentation. Local or regional SRC prior review is not required • A medical or mental health professional. The medical or mental
for human studies previously reviewed and approved bya properly constituted health professional may be a medical doctor, nurse
IRB. practitioner, physician’s assistant, doctor of pharmacy, registered nurse,
psychologist, licensed social worker or licensed
ALL projects, including those previously reviewed and approved by an IRB clinical professional counselor. The medical or mental health professional
must be reviewed and approved by the SRC after experimentation and before on the IRB may change depending on the nature of the study. This
competition in an Affiliated Fair. person must be knowledgeable about and capable of evaluating the
Projects which were conducted at a Regulated Research Institution, physical and/or psychological risk involved in a given study.
industrial setting or any work site other than home, school or field and which
were reviewed and approved by the proper institutional board before Additional Expertise: If an expert is not available in the immediate area,
experimentation, must also be approved by the Affiliated Fair SRC. documented contact with an external expert is recommended. A copy of all
An SRC must consist of a minimum of three persons, including the correspondence with the expert (e.g. emails) must be attached to Form 4 and
following: can be used in lieu of the signature of that expert.
• a biomedical scientist with an earned graduate degree
• an educator IRBs exist at federally Regulated Research Institutions (e.g., universities,
• at least one additional member medical centers, NIH, correctional facilities). Prisoner advocates must be
included on the IRB when research participants are incarcerated. The
Additional expertise: Many project evaluations require additional expertise institutional IRB must initially review and approve all proposed research
(e.g., on biosafety and/or of human risk groups). If the SRC needs an expert as conducted at or
one of its members and one is not in sponsored by that institution. The Adult Sponsor and the local IRB are
the immediate area, all documented contact with an external expert must responsible for ensuring that the project is appropriate for a pre-college student
be submitted. If animal research is involved, at least one member must be and adheres to ISEF rules.
familiar with proper animal care procedures. Depending on the nature of
the study, this person It is the responsibility of the members of the IRB to thoroughly review the
can be a veterinarian or animal care provider with training and/or experience Research Plan and collectively decide whether to approve the project, request
in the species being studied. revisions to the methodology/require more oversight (e.g., QS) to reduce risk
to participants, or to determine that the project is not appropriate for student
A Scientific Review Committee (SRC) examines projects for the research. An IRB documents the determination of risk level on Human
following: Participant Form 4.
• Evidence of proper supervision
• Completed forms, signatures, research dates, and preapproval dates (when In reviewing projects just prior to a fair, if the SRC serving at that level of
required) competition judges an IRB’s decision as inappropriate,
• Evidence of proper team composition
• Compliance with rules and laws governing human and/or

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thereby placing human participants in jeopardy, they may override the
Members of ISEF Scientific Review Committee 2020:
IRB’s decision and the project may fail to qualify for competition. It is
advised that IRBs consult with the local or affiliated fair SRCs and/or with
Ms. Susan Appel
ISEF SRC in questionable cases.
Mr. Henry Disston
Dr. Jennifer Green
Combined SRC/IRB Committee Dr. Paula Johnson Dr.
A combined committee is allowed as long as the membership meets both Timothy Martin
the SRC and IRB requirements listed previously. Mrs. Evelyn Montalvo
Mr. Joseph Scott Dr.
Jason Shuffitt
Regulated Research Institutions/ (RRI) Review Committees Mrs. Andrea Spencer
Regulated Research Institution: A Regulated Research Institution within
the U.S. is defined as a professional research/ teaching institution that is
regularly inspected by the USDA and is licensed to use animals covered by
the Animal Welfare Act and may also be subject to U.S. Public Health
Service Policy.
Also included are all federal laboratories such as National Institutes of
Health, Veteran’s Affairs Medical Centers and the Centers for Disease
Control. In addition, pharmaceutical and biotechnology companies and
research institutions that utilize research animals that are not covered by the
Animal Welfare Act but have an operational Institutional Animal Care and
Use Committee and are in compliance with U.S. federal laws are included
in this definition. For projects conducted outside of the United States, a
Regulated Research Institution would be a comparable research institution
that adheres to country laws governing the care and use of vertebrate
animals.
Certain areas of research conducted in a regulated research institution or an

industrial setting require review and approval by federally mandated
committees that have been established at that institution. These committees
include:
• Institutional Animal Care and Use Committee (IACUC); Animal Care
and Use Committee (ACUC); Animal Ethics Committee
• Institutional Review Board (IRB); Human Subjects Participant Program
(HSPP)
• Institutional Biosafety Committee (IBC)
• Embryonic Stem Cell Research Oversight Committee (ESCRO)
• Safety Review Committee
The ISEF Scientific Review Committee (ISEF SRC)

All projects are reviewed by ISEF Scientific Review Committee prior to
competition. ISEF SRC is the final arbiter of the qualification of students to
participate in ISEF. Before the fair, committee members review research plans
and all required forms to confirm that applicable ISEF rules have been
followed. ISEF SRC may request additional information from students prior to
ISEF or may interview potential ISEF participants at the fair to ensure that
they qualify to compete.
ISEF SRC, like an Affiliated Fair SRC, is made up of adults knowledgeable about
research regulations. In addition to the review of all projects at ISEF, committee
members answer questions about the rules throughout the year from students and
teachers. The ISEF SRC can be contacted at [email protected].
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HUMAN PARTICIPANTS RULES
Rules involving human participants
The following rules were developed to help pre-college student researchers
adhere to the federal regulations governing professional scientists and to A letter from an adult mentor and/or Qualified Scientist is not
protect the welfare of both human participants and the student researcher. sufficient documentation of the RRI IRB review and approval
Health and well-being is of the highest priority when students conduct process.
research with human participants.
3. The student must comply with all determinations made by the School or
According to Code of Federal Regulation 45, CFR 46, a human participant RRI IRB before beginning any interaction with human participants (e.g.,
is a living individual about whom an investigator conducting research recruitment, data collection).
obtains (1) data or samples through intervention or interaction with a. If the IRB requires a Qualified Scientist (QS), Form 2 must be
individuals(s) or (2) identifiable private information. completed by the QS before any interaction with human
participants. The School IRB will review this completed form
Examples of projects that are considered “human participant research” before approving the project.
include: b. If the IRB requires a Designated Supervisor (DS), Form 3 must be
• Participants in physical activities (e.g., physical exertion, ingestion completed before any interaction with human participants. The
of any substance, any medical procedure) School IRB will review this completed form before approving the
• Psychological, educational and opinion studies (e.g., surveys, project.
questionnaires, tests) c. See rule #4 below regarding required procedures for obtaining
• Studies in which the researcher is the subject of the research informed consent/assent and/or parental permission.
• Testing of student designed invention, prototype or computer application
by human participants other than student researcher 4. Participation in research may begin only after research participants have
• Data/record review projects that include data that are not de- voluntarily given informed consent/assent (in some cases with parental
identified/anonymous (e.g., data set that includes name, birth date, phone permission). Adult research
number or other identifying variables) participants may give their own consent. Research participants under 18
• Behavioral observations that years of age and/or individuals not able to give consent (e.g.
a. involve any interaction with the observed individual(s) or where the developmentally disabled individuals) give their assent, with the
researcher has modified the environment (e.g., post a sign, place an parent/guardian providing permission.
object).
b. occur in non-public or restricted access settings (e.g., day care The School IRB will determine whether the consent/assent/ parental
setting, doctor’s office) permission may be a) verbal or implicit or b) must be written. See the
c. involve the recording of personally identifiable Risk Assessment information on page 9 and the online Risk Assessment
information. Guide (https://sspcdn.blob.core.
windows.net/files/Documents/SEP/ISEF/Resources/Risk- Assessment-
Rules Guide.pdf) for further explanation of informed consent.
1. Student researchers must complete ALL elements of the Human a. Informed consent requires that the researcher provides complete
Participants portion of the Research Plan/Project Summary Instructions information to the participant (and where applicable, parents or
and evaluate and minimize the physical, psychological and privacy risks to guardians) about the risks and benefits associated with participation
their human participants. See Risk Assessment information on page 11 and in the research study, which then allows the participants and parents
the online Risk Assessment Guide (https://student.societyforscience. or guardians to make an informed decision about whether or not to
org/human-participants#riskassess) for additional guidance. participate.
b. Participants must be informed that their participation is voluntary
2. Student research involving human participants must be reviewed and and that they are free to stop participating at any time (i.e., they
approved by an Institutional Review Board (IRB) (See page 5) before may participate or decline to participate, with no adverse
any interaction (e.g., recruitment, data collection) with human consequences of non- participation or aborted participation).
participants may begin. It is the responsibility of the IRB to evaluate c. Informed consent may not involve coercion.
potential physical and/or psychological risks of the project and make a d. When written parental permission is required and the study
determination includes a survey, the survey must be attached to the consent
about whether the project is appropriate for student research and safe for form.
the student researcher and participants. e. The student researcher may request that the IRB waive the
a. Projects that are conducted at school, at home or in the requirement for written informed consent/parental permission in
community that are not affiliated with a Regulated his/her research plan if the project meets specific requirements. See
Research Institution (RRI) must be reviewed and approved by the section on IRB waivers for more information about situations in
School IRB before the student may begin recruiting and/or interacting which written parental permission and/or written informed consent
with human participants. The School IRB must assess the risk and can be waived by the IRB.
document its determination of risk on Form 4.
b. Projects that are conducted at a Regulated Research Institution (RRI) 5. The research study must be in compliance with all privacy laws (e.g., U.S.
(e.g., university, hospital, medical center, government lab) must have Family Educational Rights and Privacy Act (FERPA) and the U.S. Health
IRB approval from the RRI. A copy of the IRB approval for the Insurance Portability and Accountability Act (HIPAA)) when they apply to
project must be obtained. the project (e.g. the project involves medical information).

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6. Students are prohibited from independently diagnosing disease,
participants’ behavior and the study does not involve more than
administering medication, and/or performing medical procedures on
minimal risk.
human participants.
3. Surveys, questionnaires, or activities that are determined by the IRB to
a. A student may observe and collect data for analysis of medical
involve perception, cognition, or game theory,
procedures, medication/treatment efficacy, and diagnosis of illness,
etc. and that do NOT involve gathering personal information, invasion of
only under the direct supervision of a licensed health care
privacy or potential for emotional distress.
provider/professional.
b. This Healthcare provider/professional must be named in the research
4. Studies involving physical activity where the IRB determines that no more
plan/ protocol approved by the IRB. The IRB must also confirm that
than minimal risk exists and where the probability and magnitude of harm
the student is not violating the appropriate practice act (medical,
or discomfort anticipated in the research are not greater than those
nursing, pharmacy, etc) of the state or country in which he/she is
ordinarily encountered in DAILY LIFE or during performance of routine
conducting the research.
physical activities.
7. Student researchers may NOT publish or display information in a report
that identifies the human participants directly If there is any uncertainty regarding the appropriateness of waiving
or through identifiers linked to the participants (including photographs) written informed consent/assent/parental permission, it is strongly
without the written consent of the participant(s) (Public Health Service recommended that documentation of written informed
Act, 42, USC 241 (d)). consent/assent/parental permission be obtained.
8. All published instruments that are not in the public domain must be
Human Participant Involvement in Student-designed Invention,
administered, scored and interpreted by a Qualified Scientist as required
by the instrument publisher. Any and all use and distribution of the test
Prototype, Computer Application & Engineering/Design Projects
Student-designed invention, prototype, computer application and
must be in accordance with the publisher’s requirements, including
engineering/design projects that involve testing of the invention by any human
procurement of legal copies of the instrument.
participant require attention to the potential risks to the individual(s) testing or
trying out the invention/prototype.
9. Studies that involve the collection of data via use of the internet (e.g.,
1. IRB review and pre-approval is necessary when the student- designed
email, web-based surveys) are allowed, but researchers should be aware
invention, prototype, application, etc. is tested
that they can pose challenges in
by human participants other than the student researcher(s) or a single
a) collecting anonymous data, b) obtaining informed consent and c)
adult guardian or Adult Sponsor/QS/DS when the testing requires an
ensuring that participants are of the appropriate age to give informed
adult tester. This includes surveys conducted regarding potential use,
consent. See the Online Survey Consent Procedures (
review of the product and/or opinions regarding the project as defined
https://sspcdn.blob.core.windows.net/files/
by the FDA
Documents/SEP/ISEF/Resources/Online-Survey-Consent-
or Medical Practices Act) and is tested on human participants must be
Procedures.pdf).
supervised by a Qualified Scientist.
2. A Risk Assessment Form 3 is recommended for all student- designed
10. After initial IRB approval, a student with any proposed changes in the
inventions or prototypes.
Research Plan must repeat the approval process and regain approval before
resuming interaction (recruitment, data collection) with human Exempt Studies (Do Not Require IRB Preapproval or
participants. Human Participants Paperwork)
Some studies involving humans are exempt from IRB pre-approval or
11. After experimentation and before competition, the Affiliated Fair SRC additional human participant forms. Exempt projects for ISEF and affiliated
will review for compliance with all rules. fairs are:
1. Student-designed Invention, Prototype, Computer Applications or
12. The following forms are required for studies involving human Engineering/Design Project in which the student researcher is the only
participants: person testing the invention, prototype or computer application and the
a. Checklist for Adult Sponsor (1), Student Checklist (1A), Research testing does not pose a health or safety hazard. The only exception is
Plan/Project Summary, and Approval Form (1B) a single adult guardian or Adult Sponsor/QS/DS when the testing
b. Human Participants Form (4) for projects reviewed by school requires an adult tester. It is recommended that a Risk Assessment Form
IRB or IRB approval documentation from an RRI and all (3) be completed.
applicable consents and survey(s)
c. Regulated Research Institution Form (1C), when applicable 2. Data/record review studies (e.g., baseball statistics, crime statistics) in
d. Qualified Scientist Form (2), when applicable which the data are taken from preexisting data sets that are publicly
e. Risk Assessment (3) when applicable available and/or published and do not involve any interaction with
humans or the collection of any data from a human participant for the
IRB Waiver of Written Informed Consent/Parental Permission purpose of the student’s research project.
The IRB may waive the requirement for documentation of written informed
consent/assent/parental permission if the research involves only minimal risk 3. Behavioral observations of unrestricted, public settings (e.g., shopping
and anonymous data collection and if it is one of the following: mall, public park) in which all of the following apply:
1. Research involving normal educational practices a. the researcher has no interaction with the individuals being
observed
b. the researcher does not manipulate the environment in any way
2. Research on individual or group behavior or characteristics of
and
individuals where the researcher does not manipulate the
c. the researcher does not record any personally identifiable data.
Page 9
4. Projects in which the student receives pre-existing/ retrospective data in a
de-identified/anonymous format which complies with both of the
following conditions:
a. the professional providing the data certifies in writing that the data
have been appropriately de-identified before being given to the
student researcher and are in compliance with all privacy and
HIPAA laws, and
b. the affiliated fair SRC ensures that the data were appropriately
de-identified by review of the written documentation provided
by the supervising adult(s).

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HUMAN PARTICIPANT & IRB RESOURCES
Use this information to help determine the level of risk involved in a study involving human participants.
All human participant projects are considered to have some level of risk.
No more than minimal risk exists when the probability and magnitude of harm or discomfort anticipated in the research are not greater (in and of
themselves) than those ordinarily encountered in everyday life or during performance of routine physical or psychological examinations or tests.
More than minimal risk exists when the possibility of physical or psychological harm or harm related to breach of confidentiality or invasion of privacy
is greater than what is typically encountered in everyday life. Most of these studies require documented informed consent or minor assent with the
permission of parent or guardian (as applicable).
1. Examples of Greater than Minimal Physical Risk

a. Exercise other than ordinarily encountered in everyday life
b. Ingestion, tasting, smelling, or application of a substance. However, ingestion or tasting projects that involve commonly available food or drink will
be evaluated by the IRB which determines risk level based upon the nature of the study and local norms.
c. Exposure to any potentially hazardous material.
2. Examples of Greater than Minimal Psychological Risk

A research activity (e.g. survey, questionnaire, viewing of stimuli) or experimental condition that could potentially result in emotional stress.
Some examples include: answering questions related to personal experiences such as sexual or physical abuse, divorce, depression, anxiety;
answering questions that could result in feelings of depression, anxiety, or low self esteem; or viewing violent or distressing video images.
3. Privacy Concerns
a. The student researcher and IRB must consider whether an activity could potentially result in negative consequences for the participant due to
invasion of privacy or breach of confidentiality. Protecting confidentiality requires measures to ensure that identifiable research data are not
disclosed to the public or unauthorized individuals.
b. Risk level can be reduced by protecting confidentiality or collecting data that is strictly anonymous. This requires the collection of research in such a
way that it is impossible to connect research data with the individual who provided the data.
4. Risk Groups
If the research study includes participants from any of the following groups, the IRB and student research must consider whether the nature of
the study requires special protections or accommodations:
a. Any member of a group that is naturally at-risk (e.g. pregnant women, developmentally disabled persons, economically or educationally
disadvantaged persons, individuals with diseases such as cancer, asthma, diabetes, AIDS, dyslexia, cardiac disorders, psychiatric disorders,
learning disorders, etc.)
b. Special groups that are protected by federal regulations or guidelines (e.g. children/minors, prisoners, pregnant women, students receiving
services under the Individuals with Disabilities Education Act (IDEA).
See the online Risk Assessment Guide (https://sspcdn.blob.core.windows.net/files/Documents/SEP/ISEF/Resources/ Risk-Assessment-
Guide.pdf) and Online Survey Consent Procedures (https://sspcdn.blob.core.windows.net/files/ Documents/SEP/ISEF/Resources/Online-
Survey-Consent-Procedures.pdf) for more detailed information on risk assessment.
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VERTEBRATE ANIMALS RULES
Rules involving vertebrate animals
The following rules were developed to help pre-college student researchers
adhere to the federal regulations governing professional scientists and to that were considered, and the reasons these alternatives were
protect the welfare of both animal subjects and the student researcher. unacceptable; explanation of the potential impact or contribution this
Health and well-being is research may have on the broad fields of biology or medicine.
of high priority when students conduct research with animal subjects. b. Description of how the animals will be used. Include methods and
procedures, such as experimental design and data analysis;
The Society for Science & the Public strongly endorses the use of non- description of the procedures that will minimize the potential for
animal research methods and encourages students to use alternatives to discomfort, distress, pain and injury to the animals during the
animal research, which must be explored and discussed in the research plan. course of
The guiding principles for the use of animals in research include the experimentation; identification of the species, strain, sex, age,
following “Four R’s”: weight, source and number of animals proposed for use.
• Replace vertebrate animals with invertebrates, lower life forms, tissue/cell
cultures and/or computer simulations where possible. 2. All vertebrate animal studies must be reviewed and approved before
• Reduce the number of animals without compromising statistical experimentation begins. An Institutional Animal Care and Use Committee,
validity. known as an IACUC, is the institutional animal oversight review and
• Refine the experimental protocol to minimize pain or distress to the approval body for all animal studies at a Regulated Research Institution.
animals. The local OR affiliated fair SRC serves in this capacity for vertebrate
• Respect animals and their contribution to research. animals studies performed in a school, home or field. Any SRC serving in
this capacity must include a veterinarian or an animal care provider with
If the use of vertebrate animals is necessary, students must consider training and/or experience in the species being studied.
additional alternatives to reduce and refine the use of animals.
3. Students performing vertebrate animal research must satisfy US federal
All projects involving vertebrate animals must adhere to the rules for all law as well as local, state, and country laws and regulations of the
vertebrate animal studies AND to either Section A or Section B rules, jurisdiction in which research is performed.
depending on the nature of the study and the research site.
4. Research projects which cause more than momentary or slight pain or
A project is considered a tissue study and not a vertebrate animal study if distress are prohibited. Any illness or unexpected weight loss must be
tissue is obtained from an animal that was euthanized investigated and a veterinarian consulted to receive required medical care.
for a purpose other than the student’s project. (Use of tissues obtained from This investigation must
research conducted at a Regulated Research Institution requires a copy of an be documented by the Qualified Scientist or Designated Supervisor, who is
IACUC certification with the name of the research institution, the title of the qualified to determine the illness, or by a veterinarian. If the illness or
study, the IACUC approval number and date of IACUC approval.) In tissue distress is caused by the study, the experiment must be terminated
studies, a student may observe the vertebrate study, but may not manipulate immediately.
or have any direct involvement in the vertebrate animal experimental
procedures. 5. No vertebrate animal deaths due to the experimental
procedures are permitted in any group or subgroup.
a. Studies that are designed or anticipated to cause vertebrate
Vertebrate animals, as covered by these rules, are defined as: animal death are prohibited.
1. Live, nonhuman vertebrate mammalian embryos or fetuses b. Any death that occurs must be investigated by a veterinarian,
2. Tadpoles the Qualified Scientist or the Designated Supervisor who is
3. Bird and reptile eggs starting three days (72 hours) prior to hatching qualified to determine if the cause of death was incidental or
4. All other nonhuman vertebrates (including fish) at hatching or birth. due to the experimental procedures. The project must be
Exception: Because of their delayed cognitive neural development, zebrafish suspended until the cause is determined and then the results
embryos may be used up to seven days (168 hours) post-fertilization and not must be documented in writing.
be considered a vertebrate. However, regardless of time of treatment, survival c. If death was the result of the experimental procedure, the study must
past the 7 days must be considered a vertebrate animal and the entire study is be terminated, and the study will not qualify for competition.
subject to all of the rules below.
6. All animals must be monitored for signs of distress. Because significant
Rules for ALL Vertebrate Animal Studies weight loss is one sign of stress, weight must
1. All vertebrate animal studies must have a research plan that includes: be recorded at least weekly with 15% being the maximum permissible
a. Justification why animals must be used, including the reasons for the weight loss or growth retardation (compared to controls) of any
choice of species, the source of animals and the number of animals experimental or control animal. Additionally, body conditioning scoring
to be used; description, explanation, or identification of alternatives (BCS) systems are available for most species of animals utilized in
to animal use research and are an objective method for assessing the overall health
status of the research subject, with or without weight loss. A BCS system
should be included in the design of any study utilizing live vertebrate
animals and results regularly recorded.

Page
7. Students are prohibited from designing or participating in an experiment
2. Animals must be treated kindly and cared for properly. Animals must be
associated with the following types of studies on vertebrate animals:
housed in a clean, ventilated, comfortable environment appropriate for the
a. Induced toxicity studies with known toxic substances that could
species. They must be given a continuous, clean (uncontaminated) water
cause pain, distress, or death, including but not limited to
and food supply. Cages, pens and fish tanks must be cleaned frequently.
alcohol, acid rain, pesticides, or heavy
Proper care must be provided at all times, including weekends, holidays,
metals or studies with the intent to study toxic effects of a substance
and vacation periods. Animals must be observed daily to assess their
on a vertebrate animal.
health and well-being. A Designated Supervisor is required to oversee the
b. Behavioral experiments using conditioning with aversive stimuli,
daily husbandry of the animals. Any of the following U.S. documents
mother/infant separation or induced helplessness.
provide further guidance for animal husbandry:
c. Studies of pain.
• Federal Animal Welfare Regulation
d. Predator/vertebrate prey experiments.
• Guide for the Care and Use of Laboratory Animals
• Guide for the Care and Use of Agricultural Animals in
8. Justification is required for an experimental design that involves food or
Agricultural Research and Teaching (Ag-Guide)
fluid restriction and must be appropriate to the species. If the restriction
• Quality Assurance Manuals (for the appropriate species)
exceeds 18 hours, the project must be reviewed and approved by an
IACUC and conducted at a Regulated Research Institution (RRI).
3. The local or affiliated fair Scientific Review Committee must determine
if a veterinarian’s certification of the research and animal husbandry plan
9. Animals may not be captured from or released into the wild without
is required. This certification, as well as SRC approval, is required before
approval of authorized wildlife or other regulatory officials. All
experimentation and is documented on Vertebrate Animal Form 5A. A
appropriate methods and precautions must be used to decrease stress. Fish
veterinarian must certify experiments that involve supplemental nutrition,
may be obtained from the wild only if the researcher releases the fish
administration of prescription drugs and/or activities that would not be
unharmed, has the proper license, and adheres to state, local and national
ordinarily encountered in the animal’s daily life.
fishing laws and regulations. The use of electrofishing is permissible only
if conducted by a trained supervisor; students are prohibited from
4. If an illness or emergency occurs, the affected animal(s) must receive
performing electrofishing.
proper medical or nursing care that is directed by a veterinarian. A student
researcher must stop experimentation if there is unexpected weight loss or
10. A Qualified Scientist or Designated Supervisor must directly supervise all
death in the experimental subjects. The experiment can only be resumed if
research involving vertebrate animals, except for observational studies.
the cause of illness or death is not related to the experimental procedures
and if appropriate steps are taken to eliminate the causal factors. If death is
11. After initial SRC approval, a student with any proposed changes in the
the result of the experimental procedure, the study must be terminated, and
Research Plan/Project Summary of the project must repeat the
the study will not qualify for competition.
approval process before laboratory experimentation/data collection
resumes.
5. The final disposition of the animals must be conducted in a
responsible and ethical manner, and must be described on Vertebrate
A. Additional Rules for Projects Conducted at School/ Animal Form 5A.
Home/Field
Vertebrate animal studies may be conducted at a home, school, 6. Euthanasia for tissue removal and/or pathological analysis is not permitted
farm, ranch, in the field, etc. This includes: for a project conducted in a school/home/field site.
1. Studies of animals in their natural environment.
2. Studies of animals in zoological parks. 7. Livestock or fish raised for food using standard agricultural/
3. Studies of livestock that use standard agricultural aquacultural production practices may be euthanized by a qualified
practices. adult for carcass evaluation.
4. Studies of fish that use standard aquaculture practices
8. The following forms are required:
These projects must be reviewed and approved by an SRC in which one a. Checklist for Adult Sponsor (1), Student Checklist (1A), Research
member is either a veterinarian and/or an animal care provider/expert with Plan/Project Summary, and Approval Form (1B)
training and/or experience in the species being studied. b. Vertebrate Animal Form (5A)
1. These projects must adhere to BOTH of the following c. Qualified Scientist Form (2), when applicable
guidelines:
a. The research involves only agricultural, behavioral, B. Additional Rules for Projects Conducted in a
observational or supplemental nutritional studies on animals.
Regulated Research Institution
AND
All studies not meeting the criteria in Section A that are otherwise
b. The research involves only non-invasive and non- intrusive methods
permissible under ISEF rules must be conducted in a Regulated Research
that do not negatively affect an animal’s health or well-being.
Institution (RRI). A Regulated Research Institution within the U.S. is
defined as a professional research/teaching institution that is regularly
All vertebrate animal studies that do not meet the criteria in Section
inspected
A. must be conducted in a Regulated Research Institution (see
by the USDA and is licensed to use animals covered by the
Section B).
Animal Welfare Act and may also be subject to U.S.
Public Health Service Policy. Also included are all federal
laboratories such as National Institutes of Health, Veteran’s
Page 13
Affairs Medical Centers and the Centers for Disease Control. In
addition, pharmaceutical and biotechnology companies and research Exempt Studies (Do Not Require SRC Preapproval)
institutions that utilize research animals that are not covered by the 1. Studies involving behavioral observations of animals are exempt from
Animal Welfare Act but have an operational Institutional Animal Care prior SRC review if ALL of the following apply:
and Use Committee (IACUC) and are in compliance with U.S. federal a. There is no interaction with the animals being observed,
laws are included in this definition. For projects conducted outside of b. There is no manipulation of the animal environment in any way, and
the United States, a Regulated Research Institution would c. The study meets all federal and state agriculture, fish, game and
be a comparable research institution that adheres to country laws wildlife laws and regulations.
governing the care and use of vertebrate animals.
Some protocols permitted in a Regulated Research Institution

are not permitted for participation in ISEF; adherence to RRI
rules is necessary but may not be sufficient.
1. The Institutional Animal Care and Use Committee (IACUC) or the
comparable animal oversight committee must approve all student
research projects before experimentation
begins. Such research projects must be conducted under the
responsibility of a principal investigator. The local and affiliated fair
SRCs must also review the project to certify that the research project
complies with ISEF Rules. This local and regional SRC review should
occur before experimentation begins, if possible.
2. Student researchers are prohibited from performing euthanasia. Euthanasia

at the end of experimentation for tissue removal and/or pathological
analysis is permitted. All methods of euthanasia must adhere to current
American Veterinary Medical Association (AVMA) Guidelines.
3. Research projects that cause more than momentary or slight pain or

distress to vertebrate animals are prohibited unless mitigated by IACUC-
approved anesthetics, analgesics and/or tranquilizers.
4. Research in nutritional deficiency or research involving substances or

drugs of unknown effect is permitted to the point that any clinical sign of
distress is noted. In the case that distress is observed, the project must be
suspended and measures must be taken to correct the deficiency or drug
effect. A project can only be resumed if appropriate steps are taken to
correct the causal factors.

a. Checklist for Adult Sponsor (1), Student Checklist (1A), Research
Plan/Project Summary, and Approval Form (1B)
b. Regulated Research Institution Form (1C)
c. Qualified Scientist Form (2)
d. Vertebrate Animal Form (5B)
e. PHBA Risk Assessment Form (6A) – for all studies involving tissues
and body fluids.
f. Human and Vertebrate Animal Tissue Form (6B) – for all studies
involving tissues and body fluids.
Sources of Information are available as a separate section at the end of the

document.

Page
POTENTIALLY HAZARDOUS BIOLOGICAL AGENTS (PHBA)
RULES
Potentially Hazardous Biological Agents Rules for use of microorganisms (including bacteria, viruses, viroids, prions, rickettsia, fungi and
parasites), recombinant DNA technologies or human or animal fresh/frozen tissues, blood, or body fluids.
Students are permitted to do research projects with potentially hazardous

biological agents meeting the conditions and rules described below which 7. Laboratory studies designed to culture known clinically significant
were designed to protect students multidrug resistant organisms (MDROs) must have a written
and to ensure adherence to federal and international biosafety regulations justification for usage and be conducted at a Regulated Research
and guidelines. Institution laboratory with a minimum of BSL-2 containment and
documented IBC review and approval. Representative examples
When dealing with potentially hazardous biological agents, it is the include, but are not limited to the following known agents: MRSA
responsibility of the student and all of the adults involved in a research project (Methicillin- Resistant Staphylococcus aureus), VISA/VRSA
to conduct and document a risk assessment on Form (6A) to define the (Vancomycin Intermediate or Resistant Staphylococcus aureus),
potential level of harm, injury or disease to plants, animals and humans that VRE (Vancomycin-Resistant Enterococci), CRE (Carbapenem
may occur when working with biological agents. The risk assessment Resistant Enterobacteriacae), ESBLs (Extended Spectrum Beta-
determines a biosafety level which in turn determines if the project can Lactamase producing gram negative organisms), and fungi (yeasts or
proceed, and if so, the proposed laboratory facility is properly equipped and all molds) with known resistance to antifungal agents.
personnel are trained and appropriate supervision is planned.
8. Insertion of antibiotic resistance markers for the clonal selection of
All projects involving microorganisms, recombinant DNA technologies and bioengineered organisms is permitted, with the following exceptions:
human or animal fresh/frozen tissues, blood or body fluids must adhere to the a. Students are prohibited from the insertion of antibiotic- resistance
rules below AND, depending on the study, to the additional rules in Section traits or selection of organisms expressing traits that may affect the
A, B or C. ability to provide effective treatment of infections acquired by
humans, animals, or plants.
b. Students are prohibited from designing or selecting for multiple
Rules for ALL Studies with Potentially Hazardous Biological drug resistant organisms (MDROs) to investigate the pathology,
Agents (PHBA) development, or treatment of antibiotic- resistant infections.
1. Prior review and approval is required for the use of potentially hazardous
microorganisms (including bacteria, viruses, viroids, prions, rickettsia, 9. Extreme caution must be exercised when selecting and sub- culturing
fungi, and parasites), recombinant DNA (rDNA) technologies or human or antibiotic-resistant organisms. Studies using such organisms, including
animal fresh/frozen tissues, blood, or body fluids. BSL-1 organisms that may have originally been exempt from prior SRC
approval, require at least BSL-2 containment.
2. An affiliated fair SRC, an IBC or an IACUC must approve all
research before experimentation begins. The initial risk 10. The culturing of human or animal waste, including sewage sludge, is
assessment determined by the student researcher and adults supervising considered a BSL-2 study.
the project must be confirmed by the SRC, IBC or IACUC.
11. Naturally-occurring plant pathogens may be studied (not cultured) at
3. Experimentation involving the culturing of potentially hazardous home, but may not be introduced into a home/ garden environment.
biological agents, even BSL-1 organisms, is prohibited in a home
environment. However, specimens may be collected at home as long 12. All potentially hazardous biological agents must be properly disposed at
as they are immediately transported to a laboratory with the BSL the end of experimentation in accordance with their biosafety level. For
containment determined by the affiliated fair SRC. BSL 1 or BSL 2 organisms: Autoclave at 121 degrees Celsius for 20
minutes, use of a 10% bleach solution (1:10 dilution of domestic bleach),
4. Research determined to be at Biosafety Level 1 (BSL-1) must be incineration, alkaline hydrolysis, biosafety pick-up and other
conducted in a BSL-1 or higher laboratory. The research must be manufacturer recommendations are acceptable.
supervised by a trained Designated Supervisor or a Qualified Scientist.
The student must be properly trained in standard microbiological 13. Any proposed changes in the Research Plan/Project Summary by the
practices. student after initial local or affiliated fair SRC approval must undergo
subsequent SRC or IBC review and approval before such changes are
5. Research determined to be a Biosafety Level 2 (BSL-2) must be made and before experimentation resumes.
conducted in a laboratory rated BSL-2 or above (commonly limited to a
Regulated Research Institution). The research must be reviewed and 14. The following forms are required:
approved by the Institutional Biosafety Committee (IBC) if the Regulated a. Checklist for Adult Sponsor (1), Student Checklist (1A), Research
Research Institution requires the review. For a high school BSL-2 Plan/Project Summary, and Approval Form (1B)
laboratory, the SRC must review and approve. The research must be b. Regulated Research Institution Form (1C) - when
supervised by a Qualified Scientist. applicable
c. Qualified Scientist (2), when applicable
6. Students are prohibited from designing or participating in BSL- 3 or BSL-
4 Research.
Page 15
d. Risk Assessment (3), when applicable
6. Introduction or disposal of non-native, genetically-altered, and/or
e. PHBA Risk Assessment Form (6A), when applicable
invasive species (e.g. insects or other invertebrates, plants, vertebrates),
f. Human and Vertebrate Animal Tissue Form (6B) – for all studies
pathogens, toxic chemicals or foreign substances into the environment is
involving tissues and body fluids.
prohibited. Students and adult sponsors should reference their local, state
A. Additional Rules for Projects Involving Unknown and national regulations and quarantine lists.
Microorganisms
Studies involving unknown microorganisms present a challenge C. Additional Rules for Projects with Tissues and Body Fluids,
because the presence, concentration and pathogenicity of possible including Blood and Blood Products
agents are unknown. In science fair projects, these studies typically Studies involving fresh/frozen tissue, blood or body fluids obtained
involve the collection and culturing of microorganisms from the from humans and/or vertebrates may contain microorganisms and
environment (e.g. soil, household surfaces, skin.) have the potential of causing disease. Therefore, a proper risk
assessment is required.
1. Research with unknown microorganisms can be treated as a BSL-1
study under the following conditions:
1. Research involving human and/or non-human primate established cell
a. Organism is cultured in a plastic petri dish (or other standard
lines and tissue culture collections (e.g., obtained from the American
sterile non-breakable container) and sealed.
Type Culture Collection) must be considered a BSL-1 or BSL-2 level
b. Experiment involves only procedures in which the petri dish
organism as indicated by source information and treated accordingly. The
remains sealed throughout the experiment (e.g., counting presence
source and/ or catalog number of the cultures must be identified in the
of organisms or colonies).
Research Plan/Project Summary.
c. The sealed petri dish is disposed of via autoclaving or
disinfection under the supervision of the Designated
2. If tissues are obtained from an animal that was euthanized for a
Supervisor.
purpose other than the student’s project, it may be considered a tissue
study.
2. If a culture container with unknown microorganisms is opened for any
a. Use of tissues obtained from research conducted at a Regulated
purpose, (except for disinfection/disposal), it must
Research Institution requires a copy of the IACUC certification
be treated as a BSL-2 study and involve BSL-2 laboratory
with the name of the research institution, the title of the study,
precautions.
the IACUC approval number and date of IACUC approval.
b. Use of tissues obtained from agricultural/aquacultural studies
B. Additional Rules for Projects Involving Recombinant DNA require prior SRC approval.
(rDNA) Technologies
Studies involving rDNA technologies in which microorganisms, 3. If the animal was euthanized solely for the student’s project, the study
plants and/or animals have been genetically modified require must be considered a vertebrate animal project and is subject to the
close review to assess the risk level assignment. Some rDNA vertebrate animal rules. (See vertebrate animal rules.)
studies can be safely conducted in a BSL-1 high school laboratory
with prior review by a SRC. 4. The collection and examination of fresh/frozen tissue and/or body
fluids, (not including blood or blood products;
1. All rDNA technology studies involving BSL-1 organisms and see rule 7) from a non-infectious source with little likelihood of
BSL-1 host vector systems, including commercially available kits, must be microorganisms present must be considered Biosafety level 1 studies and
conducted in a BSL-1 laboratory under the supervision of a Qualified must be conducted in a BSL-1 laboratory or higher and must be supervised
Scientist or Designated Supervisor and must be approved by the SRC prior by a Qualified Scientist or trained Designated Supervisor.
to experimentation. Examples include cloning of DNA in E. coli K–12, S.
cerevesiae, and B. subtilis host- vector systems. 5. The collection and examination of fresh/frozen tissues or body fluids or
meat and meat by-products NOT obtained from food stores, restaurants,
2. An rDNA technology study using BSL-1 agents that may convert to BSL- or packing houses may contain microorganisms. Because of the
2 agents during the course of experimentation must be conducted entirely increased risk from unknown potentially hazardous agents, these studies
in a BSL-2 facility. must be considered biosafety level 2 studies conducted in a BSL-2
laboratory under the supervision of a Qualified Scientist.
3. All rDNA technology studies involving BSL-2 organisms and/or BSL-2
host vector systems must be conducted in a Regulated Research 6. Human breast milk of unknown origin, unless certified free of HIV and
Institution and approved by the IBC prior to experimentation, where Hepatitis C, and domestic unpasteurized animal milk are considered
applicable. BSL-2.
4. Propagation of recombinants containing DNA coding for human, plant or 7. All studies involving human or wild animal blood or blood products
animal toxins (including viruses) is prohibited. should be considered at a minimum a Biosafety level 2 study and must be
conducted in a BSL-2 laboratory under the supervision of a Qualified
5. All genome editing studies that include alteration of germline cells, Scientist. Known BSL-3 or BSL-4 blood is prohibited; human capillary
insertion of gene drives, use of rapid trait development systems (RTDS®), blood is exempt. Studies involving domestic animal blood may be
etc., should be categorized as a BSL-2 study and must be conducted at an considered a BSL-1 level study. All blood must be handled in accordance
RRI and approved by the IBC from the institution. Qualified scientists are with standards and guidelines set forth in the OSHA, 29CFR,
expected to ensure that student research protocols address appropriate
intrinsic and extrinsic containment precautions.

Page
Subpart Z. Any tissue or instruments with the potential of
f. Studies involving E. coli k–12 (and other strains of
containing blood-borne pathogens (e.g. blood, blood
E. coli used solely as a food source for C. elegans) that are
products, tissues that release blood when compressed, blood contaminated
performed at school and are not subject to
instruments) must be properly disposed after experimentation.
additional rules for recombinant DNA studies or use of antibiotic
resistant organisms.
8. Studies of human body fluids, where the sample can be identified with
a specific person, must have IRB review and approval, and informed
consent.
Exempt Tissues (no SRC pre-approval required)
1. The following types of tissue do not need to be treated as potentially
hazardous biological agents:
9. Any study involving the collection and examination of body fluids that
a. Plant tissue (except those known to be toxic or
may contain biological agents belonging to BSL-3 or BSL-4 is prohibited.
hazardous)
b. Plant and non-primate established cell lines and tissue culture
10. A project involving a student researcher using their own body fluids (if
collections (e.g., obtained from the American Type Culture
not cultured)
Collection). The source and/or catalog number of the cultures
a. can be considered a BSL-1 study
must be identified in the Research Plan/Project Summary.
b. may be conducted in a home setting
c. Human capillary/blood collection (.e. finger stick).
c. must have IRB review if the body fluid is serving as a measure of an
d. Fresh or frozen meat, meat by-products obtained from food
effect of an experimental procedure on the student researcher (e.g.
stores, restaurants, or packing houses and eggs or pasteurized
Student manipulates diet and takes a blood or urine sample). An
milk.
example of a project not needing IRB review would be collecting
e. Hair, hooves, nails and feathers
urine to serve as a deer repellent.
f. Teeth that have been sterilized to kill any blood-borne pathogen
d. must receive prior SRC review and approval prior to
that may be present.
experimentation.
g. Fossilized tissue or archeological specimens.
h. Prepared fixed tissue
11. Studies involving embryonic human stem cells must be conducted in a
Registered Research Institution and reviewed and approved by the
ESCRO (Embryonic Stem Cell Research Oversight) Committee. Sources of Information are available as a separate section at the end of
the document.
Exempt Studies (no SRC pre-approval required)
The following types of studies are exempt from requiring SRC pre- approval as
listed below, but may be subject to additional rules dependent upon the design
of the project. Student researchers and adult sponsors are required to refer to
sections A, B, and C
of this section to review additional rules for projects that involve unknown
organisms, recombinant DNA (rDNA) technologies, tissues, fluids, blood, or
blood products before deciding upon a final biosafety level (BSL)
designation for projects.
1. The following types of studies are exempt from prior SRC review,
but require a Risk Assessment Form 3:
a. Studies involving protists and archaea.
b. Research using manure for composting, fuel production, or other
non-culturing experiments.
c. Commercially-available color change coliform detection test kits.
These kits must remain sealed and must be properly disposed.
d. Studies involving decomposition of vertebrate organisms (such as in
forensic projects).
e. Studies with microbial fuel cells.
• The following types of studies involve BSL-1 organisms and are exempt
from prior SRC review and require no additional forms:
a. Studies involving baker’s yeast and brewer’s yeast, except in rDNA
studies.
b. Studies involving Lactobacillus, Bacillus thuringiensis,
nitrogen-fixing, oil-eating, and algae-eating bacteria introduced into
their natural environment. (Not exempt if cultured in a petri dish
environment.)
c. Studies involving water or soil microbes not concentrated in media
conducive to their microbial growth
d. Studies of mold growth on food items if the experiment is terminated
at the first evidence of mold.
e. Studies of slime molds and edible mushrooms.
Page 17
POTENTIALLY HAZARDOUS BIOLOGICAL AGENTS RISK ASSESSMENT
Use this information to complete PHBA Risk Assessment Form (6A)
Risk assessment defines the potential level of harm, injury or disease to plants,
animals and humans that may occur when working with biological agents. The 6. Assignment of a biosafety level for the study based on risk group of
end result of a risk assessment is the assignment of a biosafety level which then biological agent, level of biological containment available and the
determines the laboratory facilities, equipment, training, and supervision expertise of the Qualified Scientist or Designated Supervisor who
required. Risk assessment involves: will be supervising the project
1. Assignment of the biological agent to a risk group 7. Documentation of review and approval of study prior to
experimentation:
2. Studies involving a known microorganism must begin with an initial a. If a study is conducted at a non-regulated site (e.g. school),
assignment of the microorganism to a biosafety level risk group based on the SRC reviews the Research Plan/Project Summary.
information available through a literature search. b. If the study was conducted at a Regulated Research Institution, and
was approved by the appropriate institutional board (e.g. IBC,
3. The study of unknown microorganisms and the use of fresh tissues IACUC), the SRC reviews the institutional forms provided and
relies on the expertise of the supervising adult(s). documents SRC approval (Form(6A)).
c. If a PHBA study was conducted at a Regulated Research Institution
4. Determination of the level of biological containment available to the student but the institution does not require review for this type of study, the
researcher to conduct the experimentation. (See “Levels of Biological SRC must review the study and document approval on Form 6A
Containment” for details.) that the student received appropriate training and the project
complies with ISEF rules.
5. Assessment of the experience and expertise of the adult(s)
supervising the student.
Classification of Biological Agents

Risk Groups
ary circumstances in the research laboratory, or growth of agents in small volumes for diagnostic and experimental purposes.
ikely to cause disease in healthy laboratory workers, animals or plants. The agents require Biosafety Level 1 containment. Examples of BSL-1 organisms
a laboratory situation, the risk of spread is limited and it rarely would cause infection that would lead to serious disease. Effective treatment and preventive measu
umoniae, Salmonella choleraesuis.
erious economic consequences. Projects in the BSL-3 group are prohibited.
ntreatable. Projects in the BSL-4 group are prohibited.

Page
BSL-2 containment is designed to maximize safety when working with agents of moderate risk to humans and
the environment. Access to the laboratory is restricted. Biological safety cabinets (Class 2, type A, BSC) must be available. An autoclave should be readil
BSL-3 containment is required for infectious agents that may cause serious or potentially lethal diseases as a result of exposure by inhalation. Projects in
BSL-4 containment is required for dangerous/exotic agents that pose high risk of life-threatening disease. Projects in the BSL-4 group are prohibited.
Page 19
HAZARDOUS CHEMICALS, ACTIVITIES OR DEVICES RULES
Includes DEA-controlled substances, prescription drugs, alcohol & tobacco, firearms and explosives, radiation, lasers, etc.
The following rules apply to research using hazardous chemicals, devices and
activities. These include substances and devices that are regulated by local, Additional Rules for Specific Regulated Areas
state, country, or international law, most often with restrictions of their use by There are additional rules for the following regulated areas:
minors such as DEA- controlled substances, prescription drugs, alcohol, A. DEA-controlled Substances
tobacco, firearms and explosives. Hazardous activities are those that involve a B. Prescription Drugs
level of risk above and beyond that encountered in the student’s everyday life. C. Alcohol & Tobacco
D. Firearms and Explosives
These rules are intended to protect the student researcher by ensuring proper E. Regulated Drones
supervision and the consideration of all potential risks so that the appropriate F. Radiation
safety precautions are taken.
Students are required to meet all standards imposed by ISEF, school, A. DEA-Controlled Substances
local, and/or regional fair(s). The U.S. Drug Enforcement Administration (DEA) regulates
chemicals that can be diverted from their intended use
to make illegal drugs. Other countries may have similar regulatory
Rules for ALL Projects Involving Hazardous Chemicals, Activities bodies; students outside of the U.S. must adhere to their own
and Devices country’s drug regulatory agency requirements in addition to U.S.
1. The student researcher must conduct a risk assessment in DEA regulations. DEA- controlled substances and their schedule
collaboration with a Designated Supervisor or Qualified Scientist number are at the DEA website under Sources of Information. It is
prior to experimentation. This risk assessment should be documented the responsibility of the student to consult this list if there is a
in the research plan to include the possibility that substances used in experimentation could be
risk assessment process, supervision, safety precautions and appropriate regulated.
methods of disposal. This risk assessment is also documented on Risk 1. All studies using DEA-controlled substances must be supervised by
Assessment Form 3. a Qualified Scientist who is licensed by the DEA (or other
international regulatory body) for use of the controlled substance.
2. The use of hazardous chemicals and devices and involvement in hazardous
activities require direct supervision by a Designated Supervisor, except 2. All studies using DEA Schedule 1 substances (including
those involving DEA-controlled substances, which require supervision by marijuana) must have the research protocol approved by DEA
a Qualified Scientist. before research begins. Schedule 2, 3 and 4 substances do not
require protocol approval by DEA.
3. Student researchers must acquire and use regulated substances in
accordance with all local, state, U.S. federal and country laws. For B. Prescription Drugs
further information or classification for
Prescription drugs are regulated by federal or country laws to protect
these laws and regulations, contact the appropriate regulatory agencies.
against inappropriate or unsafe use. Special precautions must be taken
in their use for a science project as follows:
4. For all chemicals, devices or activities requiring a Federal and/ or State
1. Students are prohibited from administering prescription drugs to
Permit, the student/supervisor must obtain the permit prior to the onset of
human participants.
experimentation. A copy of the permit must be available for review by
adults supervising the project and the local, affiliated, and ISEF SRCs in
2. A veterinarian must supervise student administration of any
their review prior to competition.
prescription drugs to vertebrate animals.
5. The student researcher must minimize the impact of an
experiment on the environment. Examples include using
C. Alcohol and Tobacco
The U.S. Alcohol and Tobacco Tax and Trade Bureau (TTB) regulates the
minimal quantities of chemicals that will require
production of alcohol and distribution of alcohol and tobacco products.
subsequent disposal; ensuring that all disposal is done in an
Many such products are restricted by age for purchase, possession and
environmentally safe manner and in accordance with good laboratory
consumption.
practices.
1. Fermentation studies in which minute quantities of ethyl alcohol
are produced are permitted.
2. The Designated Supervisor is responsible for the acquisition, usage
a. Checklist for Adult Sponsor (1), Student Checklist (1A), Research
and appropriate disposal of the alcohol or tobacco used in the study.
Plan/Project Summary and Approval Form (1B)
3. Production of wine or beer by adults is allowable in the home and
b. Regulated Research Institution Form (1C), when applicable
must meet TTB home production regulations. Students are allowed
c. Qualified Scientist Form (2), when applicable
to design and conduct a research project, under direct parental
d. Risk Assessment Form (3)
supervision, involving the legal production of the wine or beer.
4. Students are prohibited from conducting experiments where
consumable ethyl alcohol is produced by

Page
distillation. However, students are allowed to distill alcohol for fuel or
4. All studies using > 25 kvolts must be conducted at an institution with
other non-consumable products. To do so,
a Licensed Radiation Program and must be preapproved by the
the work must be conducted at school or a Regulated Research
Institutions’ Radiation Safety Officer or the Committee which
Institution and follow all local and country laws. See Alcohol and
oversees the use of ionizing radiation to ensure compliance with state
Tobacco Tax and Trade Bureau (TTB) website for details.
and federal regulations.
D. Firearms and Explosives
The U.S. Bureau of Alcohol, Tobacco, Firearms and Explosives
(ATF), along with state agencies, regulates the purchase and use of
firearms and explosives. A firearm is defined as a small arms
weapon from which a projectile is fired by gunpowder. An explosive
is any chemical compound, mixture or device, the primary purpose
of which is to function by explosion. Explosives include, but are not
limited to, dynamite, black powder, pellet powder, detonators, and
igniters.
The purchase of a firearm by a minor is generally unlawful. The use of

a firearm, without proper state certification, is illegal. Students should
check the training and certification requirements of individual states
and countries.
1. Projects involving firearms and explosives are allowable when
conducted with the direct supervision of a Designated Supervisor
and when in compliance with all federal, state and local laws.
2. A fully assembled rocket motor, reload kit or propellant modules

containing more than 62.5 grams of propellant are subject to the
permitting, storage and other requirements of federal explosive
laws and regulations.
3. Potato guns and paintball guns are not considered firearms unless
they are intended to be used as weapons. However, they must be
treated as hazardous devices.
E. Regulated Drones
Projects involving unmanned aircraft systems (UAS)/drones must follow
all state, Federal and country laws. See the Federal Aviation
Administration (FAA) for more details (www.faadrone. faa.gov/#//).
F. Radiation
Projects involving radionuclides (radioisotopes) and X-rays must
involve a careful examination of the risks associated with the study
and appropriate safety precautions must be taken. Depending upon
the level of exposure, radiation released from these sources can be a
health hazard.
1. All studies may not exceed the dose limits set by the Nuclear
Regulatory Commission of 0.5 mrem/hr or 100 mrem/year of
exposure.
2. If the voltage needed in the study is <10 kvolts, a risk assessment

must be conducted. The study may be done at home or school, and
SRC preapproval is not required.
3. A study using 10–25 kvolts must have a risk assessment conducted

and must be preapproved by the SRC to assess safety. Such a study
must be conducted in a metal-lined chamber using a camera only,
not direct view through glass. A dosimeter or radiation survey meter
is required to measure radiation exposure.
Page 21
GUIDANCE FOR RISK ASSESSMENT
Please find below guidance on conducting risk assessment when using the following:
Hazardous Chemicals
Hazardous Devices
Radiation
1. Hazardous Chemicals
d. Corrosiveness — the tendency of a chemical, upon physical
A proper risk assessment of chemicals must include review of the
contact, to harm or destroy living tissues or physical
following factors:
equipment.
a. Toxicity — the tendency of a chemical to be hazardous to
human or environmental health
When assessing risk, the type and amount of exposure to a
• Human health toxicity includes acute and chronic hazards
chemical must be considered. For example, an individual’s
when inhaled, swallowed, injected or in contact with the
skin. allergic and genetic disposition may have an influence on the
• Environmental health includes aquatic toxicity (both acute and overall effect of the chemical. The student researcher must refer
chronic), toxicity to mammals and birds, and impact on to Safety Data Sheets
ecosystems. provided by the vendor (SDS) to ensure that proper safety precautions
b. Reactivity — the tendency of a chemical to undergo chemical are taken. Some SDS sheets (e.g., Flinn)
change, including instability and reactivity with other substances rank the degree of hazard associated with a chemical. This rating
or conditions (i.e., reaction with water, air, temperature, may assist students and adult sponsors in determining risk
pressure). associated with the use of a chemical.
A risk assessment (documented on Form 3) must include proper

c. Environmentally
Flammability Responsible
— the tendency Chemistry
for a chemical substance to be disposal methods for the chemicals used in an experiment. The Flinn
The mission
ignited of environmentally
at ambient temperatures.responsible
Combustible (green) chemistry
substances canis Catalog (referenced in the Sources of Information section) provides
to include:
avoid the use or production of hazardous substances during
information for the proper disposal of chemicals. If applicable, the
chemical
• process.solvents
Chemical The principles of green
that produce chemistry
vapors whichare
readily student researcher must incorporate in the research plan disposal
described on the
ignite EPA
when usedwebsite
under in the Sources
normal working of conditions.
Information
procedure required by federal and state guidelines.
section.
• Whenever possible
Combustible the following
solids (small particles,principles
powders,should
or be
incorporated into the
substances research
easily plan.
ignited by fire or an ignition source) 2. Hazardous Devices
• Waste prevention
The documentation of risk assessment (Form 3) is required when a
• Use of the safest possible chemicals and products
student researcher works with potentially hazardous/dangerous
• Design of the least possible hazardous chemical
syntheses equipment and/or other devices,
• Use renewable materials in or outside a laboratory setting that require a moderate to high
• Use catalysts in order to minimize chemical usage level of expertise to ensure their safe usage.
• Use of solvents and reaction conditions that are safe as Some commonly used devices (Bunsen burners, hot plates, saws,
possible drills, etc.) may not require a documented risk assessment,
• Maximization of energy efficiency assuming that the student researcher has experience working with
• Minimization of accident potential and avoiding the use of the device. Use of other potentially dangerous devices such as high
reactive substances vacuum equipment, heated oil baths, NMR equipment, and high
temperature ovens must have documentation of a risk assessment. It
is recommended that all student designed inventions also have
documentation of a risk assessment.
3. Radiation
A risk assessment (documented on Form 3) must be conducted
when a student’s project involves radiation beyond that normally
encountered in everyday life. Non- ionizing radiation includes the
spectrum of ultraviolet (UV), visible light, infrared (IR),
microwave (NW), radiofrequency (RF) and extremely low
frequency (ELF).

Page
ENGINEERING AND INVENTION PROJECTS GUIDE
Use this information to help determine the requirements of Engineering Projects and potential areas that will
require pre-approval and/or extra safety precautions. A Guide to Engineering & Invention Projects has been developed as an additional
resource and provides a series of questions to consider as you begin and design an engineering or invention project.
Engineering and Invention Project Checklist

Consider the answers to the questions below. If the response is yes, then the project may fall under more specific rules and those
sections of the International Rules & Guidelines should be consulted.
Hazardous Chemicals, Activities and Devices

Will your project involve any of the following:
 DEA-controlled Substances
 Firearms and Explosives
 Prescription Drugs
 Alcohol & Tobacco
 Regulated Drones
 Radiation
Device Testing with Human Participants

 Are you going to test your project (device, app, invention, prototype, etc.)? If yes, does it require persons to interact with it other than
yourself or adult sponsor/supervisor?
 Do you intend to gather background knowledge through a survey or interviews to understand the potential use and needs for your
project design?
 Are you going to ask for opinions or suggestions on your project design at any point of the project?
 Does your project intend to gather personal data/have a health benefit to the user?
Vertebrate Animals
 Does your project include any interaction with vertebrate animals in any phase of the project? If yes, please refer to the full Vertebrate
Animal Rules.
Potentially Hazardous Biological Agents

 Does your project include any collection, examination or handling of microorganisms, and/or fresh or frozen tissue, primary cell
cultures, blood, blood products or body fluids?
 Are you going to culture or isolate any substance, known or unknown? If yes, please refer to the full Potentially Hazardous Biological Agents
Rules.
Page 23
SOURCES OF INFORMATION FOR ALL PROJECTS
1. United States Patent and Trade Office Customer
Service: 1-800-786-9199 (toll-free); 571-272-1000 https://www.hhs.gov/ohrp/regulations-and-policy/
(local); 571-272-9950 (TTY) www.uspto.gov regulations/45-cfr-46
www.uspto.gov/patents/process/index.jsp
• Conducting a Patent Search - 2. NIH tutorial, “Protecting Human Research Participants”
• https://patents.google.com/ http://phrp.nihtraining.com/files/PHRP.pdf
• http://www.freepatentsonline.com/
• https://worldwide.espacenet.com/ 3. Belmont Report, April 18, 1979
www.hhs.gov/ohrp/humansubjects/guidance/belmont.html
2. USPTO Resources
• 7 Step Search Strategy Guide and Video Tutorial 4. Standards for Educational and Psychological Testing. (1999).
https://www.uspto.gov/learning-and-resources/support- Washington, DC: AERA, APA, NCME.
centers/patent-and-trademark-resource-centers-ptrc/ www.apa.org/science/programs/testing/standards.aspx
resources/seven
• https://www.youtube.com/playlist?list=PL9BtHzl4w- 5. American Psychological Association
dl2ia9lgtZ3o8KJYh_JPVQF 750 First Street, NE Washington, DC 20002-4242 phone: 202-
• https://www.uspto.gov/video/cbt/ptrcsearching/ 336-5500; 800-374-2721
• Pro Bono Program www.apa.org
https://www.uspto.gov/patents-getting-started/using- legal-
services/pro-bono/patent-pro-bono-program Information for students:
• Law School Clinic Certification Program www.apa.org/science/leadership/students/information.aspx Information
https://www.uspto.gov/learning-and-resources/ip-policy/ public- regarding publications: www.apa.org/pubs/index.aspx
information-about-practitioners/law-school-clinic-1
• USPTO Pro Se Assistance Program 6. Educational and Psychological Testing
https://www.uspto.gov/patents-getting-started/using- legal- Testing Office for the APA Science Directorate phone: 202-
services/pro-se-assistance-program 336-6000
3. European Patent Office www.epo.org www.apa.org/science/programs/testing/index.aspx
www.epo.org/applying/basics.html
7. The Children’s Online Privacy Protection Act of 1998 (COPPA) (15
4. ANS Task Force U.S.C. §§ 6501–6506) www.ftc.gov/privacy/coppafaqs.shtm
www.anstaskforce.gov
Aquatic Nuisance Species (ANS) Task Force
www.anstaskforce.gov Vertebrate Animals Animal
www.anstaskforce.gov/Documents/ISEF.pdf Care and Use
1. Laboratory Animals, Institute of Laboratory Animal Research (ILAR),
5. APHIS Commission on Life Sciences, National Research
aphis.usda.gov/aphis/home http://dels.nas.edu/ilar
Animal and Plant Health Inspection Service
Invasive Species List 2. Guide for the Care and Use of Laboratory Animals, 8th Edition (2011)
http://grants.nih.gov/grants/olaw/Guide-for-the-Care- and-Use-
6. Invasive Species Specialist Group of-Laboratory-Animals.pdf www.nap.edu/catalog.php?
www.issg.org record_id=12910
The Global Invasive Species database contains invasive species
information supplied by experts from around the world. 3. Guidelines for the Care and Use of Mammals in Neuroscience and
Behavioral Research (2003), Institute for Laboratory Animal Research
7. Invasive Species Information (ILAR) https://www.nap.edu/catalog/10732/guidelines-for-
www.invasivespeciesinfo.gov/resources/lists.shtml Provides the-care-and-use-of-mammals-in-neuroscience-and-
information for species declared invasive, noxious, prohibited, or behavioral-research
harmful or potentially harmful.
To order these ILAR publications contact: National
8. Success with Science: The Winner’s Guide to High School Academies Press
Research http://www.successwithscience.org 500 Fifth Street, NW
Gaglani, S. and DeObaldia, G. (2011). Research Corporation for Science Washington, DC 20055
Advancement. phone: 888-624-8373 or 202-334-3313; fax: 202-334-2451
ISBN 0-9633504-8-X www.nap.edu
Human Participants
1. Code of Federal Regulation (CFR), Title 45 (Public Welfare), Part 46-
Protection of Human Subjects (45CFR46)

Page
4. Federal Animal Welfare Act (AWA)
4. Quarterly bibliographies of Alternatives to the Use of Live
7 U.S.C. 2131-2157
Vertebrates in Biomedical Research and Testing may be obtained
Subchapter A - Animal Welfare (Parts I, II, III)
from:
https://www.nal.usda.gov/awic/animal-welfare-act
Specialized Information Services NLM/NIH
2 Democracy Plaza, Suite 510
Document is available from: 6707 Democracy Blvd., MSC 5467
USDA/APHIS/AC Bethesda, MD 20892-5467
4700 River Road, Unit 84
phone: 301-496-1131; Fax: 301- 480-3537
Riverdale, MD 20737-1234
https://www.sis.nlm.nih.gov
phone: 301-734-7833; fax: 301-734-4978 http://toxnet.nlm.nih.gov/altbib.html
http://awic.nal.usda.gov
5. Johns Hopkins Center for Alternatives to Animal Testing (CAAT) has
5. Guide for the Care and Use of Agricultural Animals worked with scientists since 1981 to find new methods to replace the use of
in Agricultural Research and Teaching (Agri-Guide) laboratory animals in experiments, reduce the number of animals tested,
Association for Assessment and Accreditation of Laboratory Animal and refine necessary tests to eliminate pain and distress.
Care International (AAALAC International) https://www.aaalac.org/ email: [email protected]
https://www.aaalac.org/about/Ag_Guide_3rd_ed.pdf http://caat.jhsph.edu/
6. Guidelines for the Use of Fish in Research (2014), American 6. Quality Assurance Manuals (for appropriate species) Such as:
Fisheries Society. Poultry: https://www.bordbia.ie/industry/farmers/quality/
www.fisheries.org PoultrySchemeStandards/Poultry%20Producer.pdf
www.fisheries.org/afs/docs/policy_16.pdf Beef: https://www.bqa.org/Media/BQA/Docs/
nationalmanual.pdf
7. Euthanasia Guidelines Pork: http://www.pork.org/
AVMA Guidelines on Euthanasia (2020)
American Veterinary Medical Association
www.avma.org/KB/Policies/Documents/euthanasia.pdf Potentially Hazardous Biological Agents
1. American Biological Safety Association: ABSA Risk Group
Classification — list of organisms
Alternative Research and Animal Welfare www.absa.org
1. The National Library of Medicine provides computer searches through
MEDLINE: 2. American Type Culture Collection (ATCC)
Reference & Customer Services www.atcc.org
National Library of Medicine 8600
Rockville Pike 3. Bergey’s Manual of Systematic Bacteriology website — follow the links
Bethesda, MD 20894 for resources and microbial databases for a collection of international
888-FIND-NLM or 888-346-3656; 301-594-5983; websites of microorganisms and cell cultures. https://www.bergeys.org
email: [email protected] www.nlm.nih.gov
ncbi.nlm.nih.gov/pubmed 4. Biosafety in Microbiological and Biomedical Laboratories (BMBL)
— 4th Edition. Published by CDC-NIH
2. National Agriculture Library (NAL) provides reference service for https://www.cdc.gov/labs/BMBL.html
materials that document a) Alternative Procedures to Animal Use and b)
Animal Welfare. 5. World Health Organization Laboratory Safety Manual
Animal Welfare Information Center National www.who.int/diagnostics_laboratory/guidance/en
Agriculture Library
10301 Baltimore Avenue, Room 410 6. Canada — Agency of Public Health — list of non-pathogenic
Beltsville, MD 20705-2351 organisms
phone: 301-504-6212, fax: 301-504-7125 https://www.canada.ca/en/public-health/services/ laboratory-
email: [email protected] biosafety-biosecurity/pathogen-safety-data- sheets-risk-
www.nal.usda.gov/awic assessment.html
3. Institute of Laboratory Animal Resources (ILAR) provides a • American Society for Microbiology
variety of information on animal sources, housing and https://www.asm.org
handling standards, and alternatives to animal use through annotated
bibliographies published quarterly in ILAR Journal. ILAR — The Keck • Microbiology Society
Center of the National Academies Charles Darwin House 12
500 Fifth Street, NW, Keck 687 Roger Street London
Washington, DC 20001 WC1N 2JU
phone: 202-334-2590, fax: 202-334-1687 UK
email: [email protected] http://dels.nas.edu/ilar [email protected] http://microbiologyonline.org
Page 25
7. NIH Guidelines for Research Involving Recombinant DNA
7. Alcohol, Tobacco, Firearms, and Explosives
Molecules. Published by National Institutes of Health. Alcohol and Tobacco Tax and Trade Bureau
https://osp.od.nih.gov/wp-content/uploads/2013/06/NIH_ www.ttb.gov
Guidelines.pdf Bureau of Alcohol, Tobacco, Firearms and Explosives
www.atf.gov
8. OSHA — Occupational Health and Safety Administration
www.osha.gov
8. Radiation
Radiation Studies Information (CDC)
Hazardous Chemicals, Activities or Devices www.cdc.gov/nceh/radiation/default.htm
General Lab/Chemical Safety
1. Safety in Academic Chemistry Laboratories, Volumes 1 and 2, 9. CDC Laboratory Safety Manuals
2003. Washington, DC: American Chemical Society. www.cdc.gov/biosafety/publications/index.htm
Order from (first copy free of charge):
American Chemical Society 10. Occupational Safety and Health Administration
Publications Support Services 1155 www.osha.gov
16th Street, NW Washington, DC Safety and Health Topics:
20036 www.osha.gov/SLTC
phone: 202- 872-4000 or 800-227-5558 www.osha.gov/SLTC/reactivechemicals/index.html
email: [email protected] www.osha.gov/SLTC/laserhazards/index.html
www.acs.org/education www.osha.gov/SLTC/radiationionizing/index.html
2. General 11. U.S. Nuclear Regulatory Commission

Howard Hughes Medical Institute has resources for working with cell Material Safety and Inspection Branch One
cultures, radioactive materials and other laboratory materials. White Flint North
http://www.hhmi.org/developing-scientists/resources 11555 Rockville Pike
Rockville, MD 20852
3. Environmental Protection Agency (EPA) website for green phone: 301-415-8200; 800-368-5642
chemistry www.nrc.gov
www.epa.gov/greenchemistry
4. Safety and Data Sheets (SDS)

www.flinnsci.com/msds-search.aspx
A directory of SDS sheets from Flinn Scientific Inc. that includes a
ranking of hazard level and disposal methods.
www.ilpi.com/msds/index.html - A listing of numerous sites that have

free downloads of SDS sheets.
NFPA (National Fire Protection Association) 704 Standard for guidance

on Chemical Reactivity and Instability:
https://en.wikipedia.org/wiki/NFPA_704
5. Pesticides
National Pesticide Information Center
http://npic.orst.edu/ingred/products.html
Describes the various types of pesticides and the legal requirements for
labelling. Provides links and phone numbers to get additional
information.
Environmental Protection Agency

http://iaspub.epa.gov/apex/pesticides/f?p=PPLS:1
A database of product labels. Enter the product name or company name to
view the approved label information of pesticides which are registered
with the agency.
6. DEA Controlled Substances

Drug Enforcement Agency website:
https://www.dea.gov
Controlled Substance Schedules – a list of controlled substances:
www.deadiversion.usdoj.gov/schedules

Page
ISEF DISPLAY & SAFETY REGULATIONS
Please address any questions regarding ISEF Display & Safety Regulations to
[email protected]
Display & Safety Committee Mission

The mission of this committee is to ensure that all competitors qualify for competition according to the rules established in conjunction with the Scientific Review
Committee and Society for Science & the Public.
The ISEF Display & Safety inspection process can be initiated only when all items are present at the display. The Display & Safety Committee Committee will
offer guidance on Display & Safety issues for projects approved by the SRC to compete in ISEF. Occasionally, the ISEF Display & Safety Committee may require
students to make revisions to conform to Display & Safety regulations. Persistent issues will be directed to a committee of individuals which may include Society
for Science & the Public (SSP) personnel, Display & Safety (D & S) and/or Scientific Review Committee (SRC) executive committee members.
The following regulations must be adhered to when a finalist exhibits a project at ISEF. All projects must adhere to the Display & Safety requirements of the affiliated
fair(s) in which they compete to qualify for participation in ISEF. Affiliated fairs may have additional restrictions or requirements. Knowledge of these requirements is
the responsibility of the Finalist, Adult Sponsor, and Fair Director.
DISPLAY REGULATIONS
Maximum Size of Project
Depth (front to back): 30 inches or 76 centimeters Width (side to Forms required at all projects:
side): 48 inches or 122 centimeters Height (floor to top): 108 1. An original Official Abstract and Certification as approved
inches or 274 centimeters (stamped/embossed) by the ISEF Scientific Review Committee.
a. Upon SRC approval, the stamped/embossed Official Abstract
Please be aware when ordering posters that the mechanism that supports
and Certification will be provided.
the poster should conform to the maximum size limitations stated above.
• The abstract must be the official International Science and
• All project materials and support mechanisms must fit within the project
Engineering Fair Abstract and embossed/ stamped by the ISEF
dimensions (including table covers).
Scientific Review Committee.
• No other format or version of your approved Abstract &
• Fair-provided tables at ISEF will not exceed a height of 36 inches
Certification will be allowed for any purpose at ISEF. Abstract
(91 centimeters).
handouts to judges and to the public are limited to
UNALTERED photocopies of the official abstract and
• If a table is used it becomes part of the project and must not exceed the
certification.
allowed dimensions.
b. The term “abstract” may NOT be used as a title or reference for
any information on a finalist’s display or materials at the project
• Nothing can be attached to the rear curtain.
except as part of displaying the official stamped/embossed
abstract.
• All demonstrations must be done within the confines of the finalist’s
• It is the recommendation of the Display & Safety Committee
booth space. When not being demonstrated, all project components
to NOT include the word “abstract” nor the abstract itself
must be returned to the project display and must fit within allowable
when preparing backboards or posters prior to the fair.
dimensions as defined above.
However, it is reasonable to leave a blank space (8 ½” x 11”)
on the backboard/ poster so as to facilitate the addition of the
• Projects can be continued under the table BUT this area is not to be used
official abstract. Keep in mind this document can also be
for storage.
displayed vertically on the front edge of the table or in a free-
Position of Project standing acrylic frame.
The fair provided table or freestanding display must be parallel to, and
positioned at, the back curtain of the booth. Projects may NOT lean against 2. ISEF Project Set-up Approval Form (received on-site at the Fair)
the back curtain. a. This form documents the project as approved by the Scientific
Review Committee and is used to document the Display & Safety
Committee’s review process and final approval.
Forms Required to be Visible and Vertically Displayed at the b. This form must be signed by the finalist and the Display & Safety
Project Booth Committee member at the time of inspection.
The placement of the required forms may include the front edge of the table,
the display board, or in a free-standing acrylic frame placed on the table top. Additional Forms required (only when applicable):
1. Regulated Research Institutional/Industrial Setting Form (1C)
a. If work was conducted in a regulated research institution, industrial
setting or any work site other than home, school or field at any time
during the current ISEF project year, the Regulated Research
Institutional/Industrial Setting Form (1C) must be completed and
vertically displayed at the project booth.
Page 27
b. The information provided by the mentor on Form 1C may be
must be available upon request during the set-up and inspection
referenced to confirm that the information provided on the project
process, but may not be displayed
board is that of the finalist. Only minimal reference to a mentor’s or
c. Sample release text: “I consent to the use of visual images (photos,
another researcher’s work is allowable and must only reflect
videos, etc.) involving my participation/my child’s participation in
background information or be used to clarify differences between
this research.”
finalist’s and others’ work.
2. Finalists using any presentation or demonstration outside of a project
2. Continuation/Research Progression Projects Form (7)
board must be prepared to show the entire presentation to the Display
a. If a study is a continuation/research progression, the
& Safety Inspectors before the project is approved. All
Continuation/Research Progression Projects Form (7) must be
aforementioned rules apply to this
completed and vertically displayed at the project booth.
presentation and the presentation may not be altered in any way after the
b. The display board and abstract must reflect only the current year’s
final Display & Safety inspection. Examples of presentations that require
work. The project title displayed in the finalist’s booth may mention
approval include, but are not limited to PowerPoint, Prezi, Keynote,
years of continuing research (for example, “Year Two of an
software program/simulation and other images and/or graphics displayed
Ongoing Study”).
on a computer screen or other non-print delivery method.
c. Reference to past work on the display board must be limited to
summative past conclusory data and its comparison to the current
year data set. No raw data from previous years may be publicly
Items/Materials Not Allowed on Display or at Project Booth
displayed; however, it may be included in the student research
1. Any information on the project display or items that are
notebooks and/or logbooks if properly labeled.
acknowledgments, self-promotions or external endorsements
Forms Required at Project but not Displayed are not allowed in the project booth.
1. Forms, excluding those listed above, that were required for the Scientific a. The use of logos including known commercial brands, institutional
Review Committee approval should not be vertically displayed, but must crests or trademarks, flags unless integral to the project and
be available in the booth in case asked for by a judge or other ISEF approved by the SRC via inclusion in the Official Abstract and
official. These forms include, but are not limited to, Checklist for Adult Certification.
Sponsor (1), Student Checklist (1A), Research Plan, Approval Form (1B), b. Personalized graphic/logos that are developed to indicate a
and a photograph/video release form. commercial purpose or viability of an established or proposed
business associated with the project. The only exception is a student-
2. A photograph/video release form signed by the subject is required for created logo may be displayed at the project once.
visual images of humans (other than the finalist) displayed as part of the c. Any reference to an institution or mentor that supported the
project. finalist’s research except as provided in the official ISEF
paperwork, most notably Form 1C.
d. Any reference to patent status of the project.
Forms NOT to be at the Project Display Booth or in the Exhibit
e. Any items intended for distribution such as disks, CDs, flash drives,
Hall brochures, booklets, endorsements, give- away items, business cards,
Completed informed consent/assent forms for a human participant study are printed materials or food items designed to be distributed to judges
NOT to be displayed and should NOT be present at the project display. The or the public. Once again, handouts to judges and to the public are
Finalist may include a sample (incomplete) form in their logbook or research limited to UNALTERED photocopies of the official abstract and
notebook but under NO CIRCUMSTANCE should the completed informed certification.
consent/assent forms for a human participant be in the Exhibit Hall.
2. Any awards or medals, except for past or present ISEF medals that may
Photograph/Image Display Requirements be worn by the finalist.
1. Any photograph/visual image/chart/table and/or graph is allowed if:
a. It is not deemed offensive or inappropriate (which includes 3. Postal addresses, World Wide Web, email and/or social media addresses,
images/photographs showing invertebrate or vertebrate QR codes, telephone and/or fax numbers of a project or finalist. Note:
animals/humans in surgical, necrotizing or dissection situations) by The only personal information that
the Scientific Review Committee, the Display & Safety Committee, is permissible to include on the display is information that is also included
or Society for Science & the Public. on the Official Abstract and Certification (Finalist Name, School, City,
b. It has a credit line of origin (“Photograph taken by...” or State, Country). Information regarding finalist’s age and grade are not
“Image taken from...” or “Graph/Chart/Table taken from…”). If permitted.
all images, etc. displayed were created by
the finalist or are from the same source, one credit line prominently 4. Active Internet or email connections as part of displaying or operating
and vertically displayed on the backboard/ poster or tabletop is the project at ISEF.
sufficient. All images MUST BE properly cited. This includes
background graphics, photographs and/or visual depictions of the 5. Any changes, modifications, or additions to projects including any attempt
finalist or photographs and/or visual depictions of others for which a to uncover, replenish or return removed language or items after the
signed photo/video release form is in a notebook or logbook at the approval by the Display & Safety Committee and the Scientific Review
project booth. These signed release forms Committee has been received is prohibited.
a. Display & Safety inspections will include recording photographic
evidence of the approved Project Display and Project booth.

Page
b. Finalists who do not adhere to this signed agreement on the ISEF
18. Any apparatus with belts, pulleys, chains, or moving parts with tension or
Project Set-up Approval Form regarding this regulation may fail
pinch points that are not appropriately shielded
to qualify for competition. I/we understand that the initial Display
& Safety Inspection has been completed, but that additional
19. Any display items that are deemed distracting (i.e. sounds, lights,
reviews occur and that I/we should check back regularly. I/we will
odors, etc.)
vertically display this signed form at our project at all times. I/we
have not and will not store packing material under the booth. I/we
20. Personal items or packaging materials stored underneath the booth
further understand that returning
items that have been removed by the SRC or D&S and/or adding
21. Any apparatus or project material deemed unsafe by the Scientific Review
items that are not permitted after final clearance are grounds for
Committee, the Display & Safety Committee, or the Society
failing to qualify for competition and/or forfeiture of all awards
received. Electrical Regulations
1. Electrical power supplied to the project is 120 or 220 Volt, AC, single
SAFETY REGULATIONS phase, 60 Hz. No multi-phase will be available or shall be used.
Not Allowed at Project or Booth Maximum circuit amperage/wattage available
Note: In the case in which a Finalist’s Project includes an item that is is determined by the electrical circuit capacities of the exhibit hall and may
prohibited from display, please consider taking photographs and/or be adjusted on-site by the Display & Safety Committee. For all electrical
documenting the significance of the prohibited item through video. regulations, “120 Volt AC” or “220 Volt AC” is intended to encompass the
1. Living organisms, including plants corresponding range of voltage as supplied by the facility in which ISEF is
being held.
2. Glass
2. Electrical devices must be protectively enclosed. Any enclosure must be
non-combustible. All external non-current carrying metal parts must be
3. Soil, sand, rock, cement and/or waste samples, even if
grounded.
permanently encased in a slab of acrylic
3. Energized wiring, switches, and metal parts must have adequate
4. Taxidermy specimens or parts
insulation and over-current safety devices (such as fuses) and must be
inaccessible to anyone other than the finalist. Exposed electrical
5. Preserved vertebrate or invertebrate animals
equipment or metal that may be
energized must be shielded with a non-conducting material or with a
6. Human or animal food
grounded metal box to prevent accidental contact.
7. Human/animal parts or body fluids (for example, blood, urine)
4. Decorative lighting or illumination is discouraged. If used, lighting
must be as low a voltage as possible and must be LED lighting that
8. Plant materials (living, dead, or preserved) that are in their raw,
does not generate heat. Incandescent and fluorescent light bulbs are
unprocessed, or non-manufactured state prohibited. When student is not at the exhibit, all electrical power must
be disconnected, or power bars must be switched off (Exception:
9. All chemicals including water. Absolutely no liquids can be utilized in during pre- judging audio visual displays may be available.)
the Project Display
5. An insulating grommet is required at the point where any wire or cable
10. All hazardous substances or devices (Example: poisons, drugs, firearms, enters any enclosure.
weapons, ammunition, reloading devices, grease/oil and sublimating solids
such as dry ice) 6. No exposed live circuits over 36 volts are allowed.
11. Items that may have contained or been in contact with hazardous
7. There must be an accessible, clearly visible on/off switch or other means
chemicals (Exception: Item may be permitted if professionally cleaned
of quickly disconnecting from the 120 or 220 Volt power source.
and documentation for such cleaning is available). Filters (including
microbial) may not be displayed unless the Display & Safety Committee Laser/Laser Pointer Regulations
can reasonably determine that the device was cleaned or was never used Any Class 1, Class 2, Class 3A, or Class 3R lasers are allowed to be used
(please include receipts in your notebooks and/or logbooks) responsibly. No other lasers may be used or displayed.
1. Laser beams may not pass through magnifying optics such as microscopes
12. Sharp items (for example, syringes, needles, pipettes, knives) and telescopes. Lasers must be labeled by the manufacturer so that power
output can be inspected. Lasers without labels will NOT be permitted.
13. Flames and highly flammable materials
2. Lasers must be labeled by the manufacturer so that power output can
14. Batteries with open-top cells or wet cells be inspected. Lasers without labels will NOT be permitted.
15. Drones or any flight-capable apparatus unless the propulsion power 3. Handheld lasers are NOT permitted.
source removed.
4. Lasers will be confiscated with no warning if not used in a safe manner.
16. 3D Printers unless the power source is removed.
17. Inadequately insulated apparatus capable of producing dangerous

temperatures are not permitted
Page 29
ISEF CATEGORIES AND SUBCATEGORIES
The categories have been established with the goal of better aligning judges and student projects for the judging at ISEF. Local, regional, state
and country fairs may or may not choose to use these categories, dependent on the needs of their area. Please check with your affiliated fair(s)
for the appropriate category listings at that level of competition.
Please visit our website at student.societyforscience.org/intel-isef-categories-and-subcategories for a full description and

definition of ISEF categories:
ANIMAL SCIENCES (ANIM) CHEMISTRY (CHEM) ENGINEERING PHYSICS AND
Animal Behavior Analytical Chemistry MECHANICS (ENMC) ASTRONOMY (PHYS)
Cellular Studies Computational Chemistry Aerospace and Aeronautical Astronomy and Cosmology Atomic,
Development Environmental Chemistry Engineering Molecular, and Optical
Ecology Genetics Inorganic Chemistry Materials Civil Engineering Computational Physics Biological
Nutrition and Growth Chemistry Organic Chemistry Mechanics Control Theory Physics
Physiology Physical Chemistry Ground Vehicle Systems Condensed Matter and
Systematics and Evolution Other Industrial Engineering- Materials
Other Processing Mechanical Mechanics
COMPUTATIONAL BIOLOGY Engineering Naval Systems Nuclear and Particle Physics Theoretical,
BEHAVIORAL AND SOCIAL AND BIOINFORMATICS Other Computational and
SCIENCES (BEHA) (CBIO) Quantum Physics Other
Clinical and Developmental Computational Biomodeling ENVIRONMENTAL
Psychology Computational Epidemiology ENGINEERING (ENEV) PLANT SCIENCES (PLNT)
Cognitive Psychology Computational Evolutionary Bioremediation Land Agriculture and Agronomy
Neuroscience Physiological Biology Reclamation Pollution Ecology Genetics/Breeding
Psychology Computational Neuroscience Control Growth and Development
Sociology and Social Psychology Computational Pharmacology Recycling and Waste Pathology
Other Genomics Management Plant Physiology Systematics
Other Water Resources Management Other and Evolution Other
BIOCHEMISTRY (BCHM)
Analytical Biochemistry EARTH AND MATERIALS ROBOTICS AND
General Biochemistry Medical SCIENCE (MATS) INTELLIGENT MACHINES
Biochemistry Structural
ENVIRONMENTAL
SCIENCES (EAEV) Biomaterials Ceramic (ROBO)
Biochemistry Other and Glasses Composite
Atmospheric Science Climate Biomechanics
Science Environmental Effects Materials Cognitive Systems
BIOMEDICAL AND HEALTH Computation and Theory Electronic, Control Theory
on
SCIENCES (BMED) Ecosystems Optical and Magnetic Machine Learning
Cell, Organ, and Systems Physiology Materials Robot Kinematics
Geosciences Water
Genetics and Molecular Biology of Nanomaterials Other
Science Other
Disease Polymers Other
Immunology SYSTEMS
EMBEDDED SYSTEMS (EBED)
Nutrition and Natural Products MATHEMATICS (MATH) SOFTWARE (SOFT)
Circuits
Pathophysiology Analysis
Internet of Things Algorithms
Other Combinatorics, Graph Theory, and
Microcontrollers Networking Cybersecurity
and Data Game Theory Databases
BIOMEDICAL ENGINEERING Communications Optics Geometry and Topology Human/Machine Interface Languages and
(ENBM) Sensors Number Theory Probability Operating
Biomaterials and Regen Medicine Signal Processing and Statistics Other Systems Mobile
Biomechanics Other Apps Online
Biomedical Devices MICROBIOLOGY (MCRO) Learning Other
Biomedical Imaging ENERGY: SUSTAINABLE Antimicrobials and Antibiotics Applied
Cell and Tissue Engineering Synthetic Microbiology Bacteriology TRANSLATIONAL MEDICAL
MATERIALS AND DESIGN
Biology Environmental Microbiology SCIENCES (TMED)
Other (EGSD)
Microbial Genetics Disease Detection and
Biological Process and Design Energy
Virology Diagnosis
Storage
CELLULAR AND Hydrogen Generation and Storage Other Disease Prevention Disease
MOLECULAR BIOLOGY OtherThermal Power Treatment and
(CELL) Solar Process, Materials, and Design Therapies
Cell Physiology Cellular Thermal Generation and Design Drug Identification and Testing Pre-
Immunology Genetics Triboelectricity and Electrolysis Wind Clinical Studies
Molecular Biology Wind and Water Movement Power Other
Neurobiology Other Generation
Other

Page
INFORMATION ON REQUIRED ABSTRACT & CERTIFICATION FOR ALL PROJECTS AT
ISEF
* This form may not be relevant for your regional or state fair; please refer to instructions from your affiliated fair.*
In ADDITION to the basic form requirements for ALL Projects and any other requirements due to specific areas of research, an Abstract
& Certification is required at the conclusion of research. Details on this requirement follow.
Completing the Abstract

ISEF Sample Abstract & Certification
After finishing research and experimentation, you
are required to write a (maximum) 250 word, one-
PROJECT TITLE
PROJECT ID
page abstract. For ISEF, this abstract is written in the
online Finalist Questionnaire portal and submitted FINALIST NAME(S)
Category
electronically. FINALIST SCHOOL, CITY, STATE/PROVINCE, COUNTRY Pick one only-- mark an “X” in
box at right
It is recommended that it include the

following: ABSTRACT BODY
a. purpose of the experiment
b. procedure
c. data
d. conclusions
It may also include any possible research

applications. Only minimal reference to previous
work may be included.
An abstract must not include the following:

a. acknowledgments (including naming
the research institution and/or mentor 1. As a part of this research project, the student directly handled, manipulated, or interacted
with which you were working), or self- with (check all that apply):
promotions and external endorsements
b.logos or proper names of commercial  human participants  potentially hazardous biological agents
products  vertebrate animals  microorganisms  rDNA  tissue
b. work or procedures done by the
mentor 2. This abstract describes only procedures performed by me/us, reflects my/ our own
independent research, and represents one year’s work only.
Completing the Certification  yes  no
At the bottom of the Abstract & Certification form 3. I/We worked or used equipment in a regulated research institution or industrial
there are six questions. Please read each carefully setting.
and answer appropriately. The ISEF Scientific  yes  no
Research Committee will review and approve the
abstract and answers to the questions.
4. This project is a continuation of previous research.
Revisions are permitted via the online portal  yes  no
through late April (please reference the system for
current year deadlines.)
5. My display board includes non-published photographs/visual depictions of humans (other
Once approved, two copies of the ISEF Abstract & than myself):
Certification will be provided with a gold embossed  yes  no
seal; only this version of the abstract may be
displayed or distributed. 6. I/We hereby certify that the abstract and responses to the above statements are correct and
properly reflect my/our own work.
NOTE: Your abstract must be on the International  yes  no
Science and Engineering Fair Abstract &
Certification form and have the ISEF Scientific
Review Committee approval seal before it is
displayed or handed
FOR ISEF OFFICIAL USE ONL
out. No other format or version of your approved
Abstract will be allowed for any purpose at the
ISEF.
Page 31
Checklist for Adult Sponsor (1)
This completed form is required for ALL projects.
To be completed by the Adult Sponsor in collaboration with the student researcher(s):
Student’s Name(s):
Project Title:
1.  I have reviewed the ISEF Rules and Guidelines, including the science fair ethics statement.
2.  I have reviewed the student’s completed Student Checklist (1A) and Research Plan/Project Summary.
3.  I have worked with the student and we have discussed the possible risks involved in the project.
4.  The project involves one or more of the following and requires prior approval by an SRC, IRB, IACUC or IBC:
 Humans Potentially Hazardous Biological Agents
 Vertebrate Animals  Microorganisms  rDNA  Tissues
5.  Items to be completed for ALL PROJECTS

 Adult Sponsor Checklist (1)  Research Plan/Project Summary
 Student Checklist (1A)  Approval Form (1B)
 Regulated Research Institutional/Industrial Setting Form (1C) (when applicable; after completed experiment)
 Continuation/Research Progression Form (7) (when applicable)
Additional forms required if the project includes the use of one or more of the following (check all that apply):
 Humans, including student designed inventions/prototypes. (Requires prior approval by an Institutional Review Board (IRB); see full text of the rules.)
 Human Participants Form (4) or appropriate Institutional IRB documentation
 Sample of Informed Consent Form (when applicable and/or required by the IRB)
 Qualified Scientist Form (2) (when applicable and/or required by the IRB)
 Vertebrate Animals (Requires prior approval, see full text of the rules.)
 Vertebrate Animal Form (5A) - for projects conducted in a school/home/field research site (SRC prior approval required.)
 Vertebrate Animal Form (5B) - for projects conducted at a Regulated Research Institution. (Institutional Animal Care and Use Committee
(IACUC) approval required prior experimentation.)
 Qualified Scientist Form (2) (Required for all vertebrate animal projects at a regulated research site or when applicable)
 Potentially Hazardous Biological Agents (Requires prior approval by SRC, IACUC or IBC, see full text of the rules.)
 Potentially Hazardous Biological Agents Risk Assessment Form (6A)
 Human and Vertebrate Animal Tissue Form (6B) - to be completed in addition to Form 6A when project involves the use of fresh or frozen tissue,
primary cell cultures, blood, blood products and body fluids.
 Qualified Scientist Form (2) (when applicable)
 The following are exempt from prior review but require a Risk Assessment Form 3: projects involving protists, archae and similar microorganisms, for
projects using manure for composting, fuel production or other non-culturing experiments, projects using color change coliform water test kits,
microbial fuel cells, and projects involving decomposing vertebrate organisms.
 Hazardous Chemicals, Activities and Devices (No SRC prior approval required, see full text of the rules.)
 Risk Assessment Form (3)
 Qualified Scientist Form (2) (required for projects involving DEA-controlled substances or when applicable)
 Other
 Risk Assessment Form (3)
 I attest to the information checked above and that I have read and agree to abide by the science fair ethics statement.
Adult Sponsor’s Printed Name Signature Date of Review (mm/dd/yy)
Phone Email

Page
Student Checklist (1A)
This form is required for ALL projects.
1. a. Student/Team Leader: Grade:
Email: Phone:
b. Team Member: c. Team Member:
2. Title of Project:
3. School: School Phone:
School Address:
4. Adult Sponsor: Phone/Email:
5. Does this project need SRC/IRB/IACUC or other pre-approval?  Yes  No Tentative start date:
6. Is this a continuation/progression from a previous year?  Yes  No

If Yes:
a. Attach the previous year’s  Abstract and  Research Plan/Project Summary
b. Explain how this project is new and different from previous years on
 Continuation/Research Progression Form (7)
7. This year’s laboratory experiment/data collection:
Actual Start Date: (mm/dd/yy) End Date: (mm/dd/yy)
8. Source of Data:
 Collected self/mentor  Other Describe/url:
9. List name and address of all non-home and non-school work site(s):
Name:
Address:
Phone/
email
10. Complete a Research Plan/Project Summary following the Research Plan/Project Summary instructions and attach to
this form.
11. An abstract is required for all projects after experimentation.
Page 33
Research Plan/Project Summary Instructions
A complete Research Plan/Project Summary is required for ALL projects and must accompany Student Checklist (1A).
• All projects must have a Research Plan/Project Summary

a. Written prior to experimentation following the instructions below to detail the rationale, research question(s), methodology, and risk assessment of the
proposed research.
b. If changes are made during the research, such changes can be added to the original research plan as an addendum, recognizing that some changes may
require returning to the IRB or SRC for appropriate review and approvals. If no additional approvals are required, this addendum serves as a project
summary to explain research that was conducted.
c. If no changes are made from the original research plan, no project summary is required.
• Some studies, such as an engineering design or mathematics projects, will be less detailed in the initial project plan and will change through the course of
research. If such changes occur, a project summary that explains what was done is required and can be appended to the original research plan.
• The Research Plan/Project Summary should include the following:

a. RATIONALE: Include a brief synopsis of the background that supports your research problem and explain why this research is important and if
applicable, explain any societal impact of your research.
b. RESEARCH QUESTION(S), HYPOTHESIS(ES), ENGINEERING GOAL(S), EXPECTED OUTCOMES: How is this based on the
rationale described above?
c. Describe the following in detail:
• Procedures: Detail all procedures and experimental design including methods for data collection, and when applicable, the source of data used. Describe
only your project. Do not include work done by mentor or others.
• Risk and Safety: Identify any potential risks and safety precautions needed.
• Data Analysis: Describe the procedures you will use to analyze the data/results.
d. BIBLIOGRAPHY: List major references (e.g. science journal articles, books, internet sites) from your literature review. If you plan to use vertebrate
animals, one of these references must be an animal care reference.
Items 1–4 below are subject-specific guidelines for additional items to be included in your research plan/project summary as applicable.
1. Human participants research:
a. Participants: Describe age range, gender, racial/ethnic composition of participants. Identify vulnerable populations (minors, pregnant women,
prisoners, mentally disabled or economically disadvantaged).
b. Recruitment: Where will you find your participants? How will they be invited to participate?
c. Methods: What will participants be asked to do? Will you use any surveys, questionnaires or tests? If yes and not your own, how did you obtain? Did it
require permissions? If so, explain. What is the frequency and length of time involved for each subject?
d. Risk Assessment: What are the risks or potential discomforts (physical, psychological, time involved, social, legal, etc.) to participants? How
will you minimize risks? List any benefits to society or participants.
e. Protection of Privacy: Will identifiable information (e.g., names, telephone numbers, birth dates, email addresses) be collected? Will data be
confidential/anonymous? If anonymous, describe how the data will be collected. If not anonymous, what procedures are in place for safeguarding
confidentiality? Where will data be stored? Who will have access to the data? What will you do with the data after the study?
f. Informed Consent Process: Describe how you will inform participants about the purpose of the study, what they will be asked to do, that their
participation is voluntary and they have the right to stop at any time.
2. Vertebrate animal research:

a. Discuss potential ALTERNATIVES to vertebrate animal use and present justification for use of vertebrates.
b. Explain potential impact or contribution of this research.
c. Detail all procedures to be used, including methods used to minimize potential discomfort, distress, pain and injury to the animals and detailed
chemical concentrations and drug dosages.
d. Detail animal numbers, species, strain, sex, age, source, etc., include justification of the numbers planned.
e. Describe housing and oversight of daily care.
f. Discuss disposition of the animals at the end of the study.
• Potentially hazardous biological agents research:

a. Give source of the organism and describe BSL assessment process and BSL determination.
b. Detail safety precautions and discuss methods of disposal.
4. Hazardous chemicals, activities & devices:

• Describe Risk Assessment process, supervision, safety precautions and methods of disposal.
• Material Safety Data Sheets are not necessary to submit with paperwork.

Page
Approval Form (1B)
A completed form is required for each student, including all team members.
1. To Be Completed by Student and Parent

a. Student Acknowledgment:
 I understand the risks and possible dangers to me of the proposed research plan.
 I have read the ISEF Rules and Guidelines and will adhere to all International Rules when conducting this research.
 I have read and will abide by the science fair ethics statement.
Student researchers are expected to maintain the highest standards of honesty and integrity. Scientific fraud and misconduct are not
condoned at any level of research or competition. Such practices include but are not limited to plagiarism, forgery, use or presentation of
other researcher’s work as one’s own, and fabrication of data. Fraudulent projects will fail to qualify for competition in affiliated fairs
and ISEF.
Student’s Printed Name Signature Date Acknowledged (mm/dd/yy)

(Must be prior to experimentation.)
b. Parent/Guardian Approval: I have read and understand the risks and possible dangers involved in the
Research Plan/Project Summary. I consent to my child participating in this research.
Parent/Guardian’s Printed Name Signature Date Acknowledged (mm/dd/yy)

2. To be completed by the local or affiliated Fair SRC

(Required for projects requiring prior SRC/IRB APPROVAL. Sign 2a or 2b as appropriate.)
b.Required for research conducted at all Regulated Research Institutions wi

a. Required for projects that need prior SRC/IRB approval BEFORE
This project was conducted at a regulated research institution (not home or high
experimentation (humans, vertebrates or potentially hazardous
biological agents). OR
The SRC/IRB has carefully studied this project’s Research Plan/ Project
Summary and all the required forms are included. My signature indicates
approval of the Research Plan/Project Summary before the student begins
experimentation.
SRC/IRB Chair’s Printed Name

SRC Chair’s Printed Name
Signature Date of Approval (mm/dd/yy) (Must be

prior to experimentation.)
Signature Date of Signature (mm/dd/yy)
(May be after experimentation)
3. Final ISEF Affiliated Fair SRC Approval(Required for ALL Projects)
SRC Approval After Experimentation and Before Competition at Regional/State/National Fair

I certify that this project adheres to the approved Research Plan/Project Summary and complies with all ISEF Rules.
Regional SRC Chair’s Printed Name Signature Date of Approval (mm/dd/yy)
State/National SRC Chair’s Printed Name Signature Date of Approval (mm/dd/yy)

(where applicable)
Page 35
Regulated Research Institutional/Industrial Setting Form (1C)
R experimentation by the adult supervising the student research conducted in a regulated research institution, industrial setting or any
Student’s Name(s)
Title of Project
To be completed by the Supervising Adult in the Setting (NOT the Student(s)) after experimentation: (Responses must be on the
form as it is required to be displayed at student’s project booth; please do not print double- sided.)
The student(s) conducted research at my work site:

1. Did you or your proxy (e.g. graduate student, postdoc, employee) mentor or provide
substantial guidance to the student researcher?  Yes  No
a. If no, describe your and/or your institution’s role with the student researcher and his/her project (e.g.
supervised use of equipment on site without ongoing mentorship and sign below.
b. If yes, complete questions 2 –5.
2. Is the student’s research project a subset of your ongoing research or work?  Yes  No
Use questions 3, 4 and 5 to detail how the student’s project was similar and/or
different from ongoing research or work at your site. If this project is under a grant and needs to be
acknowledged, please list the grant statement here.
3. Describe the independence and creativity with which the student:

a. developed the hypotheses or engineering goals for the research project
b. designed the methodology for his/her research project
c. analyzed and interpreted data

Page
Regulated Research Institutional/Industrial Setting Form (1C) Continued
Student’s Name(s)
(Continued on next page)
4. Detail the student’s role in conducting the research (e.g. data collection, specific procedures performed).
Differentiate what the student observed and what the student actually did.
5. Did the student(s) work on the project as part of a group?  Yes  No

If yes, how many individuals were in the group and who were they (e.g. high school
students, graduate students, faculty, professional researchers)?
I attest that the student has conducted the work as indicated above and that any required review and approval by institutional regulatory
board (IRB/IACUC/IBC) has been obtained. Copies are attached if applicable. I further acknowledge that the student will be presenting
this work publicly in competition and I have communicated with the student research regarding any requirements for my review and/or
restrictions of what is publicized.
Supervising Adult’s Printed Name Signature Title
Institution Date Signed (must be after experimentation)

(mm/dd/yy)
Address Email/Phone
Page 37
Qualified Scientist Form (2)
an participants, vertebrate animals, potentially hazardous biological agents, and hazardous substances and devices. Must be completed and sig
Student’s Name(s)
Title of Project
To be completed by the Qualified Scientist:

Scientist Name:
Educational Background: Degree(s):
Experience/Training as relates to the student’s area of research
Position: Institution:
Address: Email/Phone:
1. Have you reviewed the ISEF rules relevant to this project and the science  Yes  No
fair ethics statement relevant to this project?
2. Will any of the following be used?

a. Human participants  Yes  No
b. Vertebrate animals  Yes  No
c. Potentially hazardous biological agents (microorganisms, rDNA and tissues, including  Yes  No
blood and blood products)
d. Hazardous substances and devices  Yes  No
3. Will this study be a sub-set of a larger study?  Yes  No

4. Will you directly supervise the student?  Yes  No
a. If no, who will directly supervise and serve as the Designated Supervisor?
b. Experience/Training of the Designated Supervisor:
To be completed by the Qualified Scientist: To be completed by the Designated Supervisor when the Qualified
I certify that I have reviewed and approved the Research Plan/ Project SummaryI prior
certifytothat
the Istart
haveofreviewed the Research Plan/Project Summary and have been
the experimentation.
If the student or Designated Supervisor is not trained in the necessary procedures, I will ensure her/his training. I will provide advice and supervision during the
that a Designated Supervisor is required when the student is not conducting experimentation under my direct supervision.
Designated Supervisor’s Printed Name
Qualified Scientist’s Printed Name Signature Date of Approval (mm/dd/yy)
Signature Date of Approval (mm/dd/yy)

Phone Email

Page
Risk Assessment Form (3)
Must be completed before experimentation.
Student’s Name(s)
Title of Project
To be completed by the Student Researcher(s) in collaboration with Designated Supervisor/Qualified Scientist: (All
questions must be answered; additional page(s) may be attached.)
1. List all hazardous chemicals, activities, or devices that will be used; identify microorganisms exempt from pre-approval (see Potentially
Hazardous Biological Agent rules).
2. Identify and assess the risks and hazards involved in this project.
3. Describe the safety precautions and procedures that will be used to reduce the risks.
4. Describe the disposal procedures that will be used (when applicable).
5. List the source(s) of safety information.
To be completed and signed by the Designated Supervisor (or Qualified Scientist, when applicable): I agree with the risk ass
Designated Supervisor’s Printed Name Signature Date of Review (mm/dd/yy)
Position & Institution Phone or email contact information
Experience/Training as relates to the student’s area of research
Page 39
Human Participants Form (4)
l research involving human participants not at a Regulated Research Institution. If at a Regulated Research Institution, use institutional approval forms for documentation of prior r
(IRB approval required before recruitment or data collection.)
Student’s Name(s) Title of Project
Adult Sponsor Phone/Email

MUST BE COMPLETED BY STUDENT RESEARCHER(S) IN COLLABORATION WITH THE ADULT SPONSOR/DESIGNATED SUPERVISOR/QUALIFIED SCIE
I have submitted my Research Plan/Project Summary which addresses ALL areas indicated in the Human Participants Section of the Research Plan/Project Summary Instructions.
I have attached any surveys or questionnaires I will be using in my project or other documents provided to human participants.
 Any published instrument(s) used was /were legally obtained.
I have attached an informed consent that I would use if required by the IRB.
 .... Yes ... NoAre you working with a Qualified Scientist? If yes, attach the Qualified Scientist Form 2.
BELOW - IRB USE ONLY

MUST BE COMPLETED BY INSTITUTIONAL REVIEW BOARD (IRB) AFTER REVIEW OF THE RESEARCH PLAN. ALL QUESTIONS
MUST BE ANSWERED FOR THE APPROVAL TO BE VALID. (IF NOT APPROVED, RETURN PAPERWORK TO THE STUDENT WITH
INSTRUCTIONS FOR MODIFICATIONS.)
 Approved with Full Committee Review (3 signatures required) and the following conditions: (All 6 must be answered)
1. Risk Level (check one) :  Minimal Risk  More than Minimal Risk
2. Qualified Scientist (QS) Required (Form 2):  Yes  No
3. Designated Supervisor (DS) Required (Form 3):  Yes  No
4. Written Minor Assent required for minor participants:
 Yes  No  Not applicable (No minors in this study)
5. Written Parental Permission required for minor participants:
 Yes  No  Not applicable (No minors in this study)
6. Written Informed Consent required for participants 18 years or older:
 Yes  No  Not applicable (No participants 18 yrs or older in this study)
IRB SIGNATURES (All 3 signatures required) None of these individuals may be the adult sponsor, designated supervisor, qualified scientist or related to (e.g.,
mother, father of) the student (conflict of interest).
I attest that I have reviewed the student’s project, that the checkboxes above have been completed to indicate the IRB determination and that I agree
with the decisions above.
Medical or Mental Health Professional (a psychologist, medical doctor, licensed social worker, licensed clinical professional counselor, physician’s assistant,
doctor of pharmacy, or registered nurse) with expertise related to this project.
Printed Name Degree/Professional License
Signature Date of Approval (Must be prior to experimentation.) (mm/dd/yy)
Educator
School Administrator

Page
Human Informed Consent Form
Instructions to the Student Researcher(s): An informed consent/assent/permission form should be developed in consultation with the
Adult Sponsor, Designated Supervisor or Qualified Scientist.
This form is used to provide information to the research participant (or parent/guardian) and to document written informed consent, minor
assent, and/or parental permission.
• When written documentation is required, the researcher keeps the original, signed form.
• Students may use this sample form or may copy ALL elements of it into a new document.
If the form is serving to document parental permission, a copy of any survey or questionnaire must be attached.
Student Researcher(s):
Title of Project:
I am asking for your voluntary participation in my science fair project. Please read the following information about the project. If you would like to
participate, please sign in the appropriate area below.
Purpose of the project:
If you participate, you will be asked to:
Time required for participation:
Potential Risks of Study:
Benefits:
How confidentiality will be maintained:
If you have any questions about this study, feel free to contact:
Adult Sponsor/QS/DS: Phone/email:
Voluntary Participation:
Participation in this study is completely voluntary. If you decide not to participate there will not be negative consequences. Please be aware that
if you decide to participate, you may stop participating at any time and you may decide not to answer any specific question.
By signing this form I am attesting that I have read and understand the information above and I freely give my consent/ assent to participate or
permission for my child to participate.
Adult Informed Consent or Minor Assent Date Reviewed & Signed:

(mm/dd/yy)
Research Participant Printed Name: Signature:
Parental/Guardian Permission (if applicable) Date Reviewed & Signed:

(mm/dd/yy)
Parent/Guardian Printed Name: Signature:

Page
Vertebrate Animal Form (5A)
r all research involving vertebrate animals that is conducted in a school/home/field research site. (SRC approval required before ex
Student’s Name(s)
Title of Project
To be completed by Student Researcher:

1. Common name (or Genus, species) and number of animals used.
2. Describe completely the housing and husbandry to be provided. Include the cage/pen size, number of animals per cage, environment,
bedding, type of food, frequency of food and water, how often animal is observed, etc. Add an additional page as necessary.
3. What will happen to the animals after experimentation?
4. Attach a copy of wildlife licenses or approval forms, as applicable
5. The ISEF Vertebrate Animal Rules require that any death, illness or unexpected weight loss be investigated and documented by a letter
from the qualified scientist, designated supervisor or a veterinarian. If applicable, attach this letter with this form when submitting your
paperwork to the SRC prior to competition.
To be completed by Local or Affiliate Fair Scientific Review Committee (SRC) BEFORE experimentation.
Level of Supervision Required for agricultural, behavioral or nutritional studies (select one):
Designated Supervisor REQUIRED. Please have applicable person sign below.
Veterinarian and Designated Supervisor REQUIRED. Please have applicable persons sign below.
 Veterinarian, Designated Supervisor and Qualified Scientist REQUIRED. Please have applicable persons sign below and have the Qualified Scientist complete Form
The SRC has carefully reviewed this study and finds it is an appropriate study that may be conducted in a non-regulated research site.
Local or Affiliate Fair SRC Pre-Approval Signature:
SRC Chair Printed Name Signature Date of Approval (must be prior to experimentation) (mm/dd/
To be completed by Veterinarian: To be completed by Designated Supervisor or Qualified Scientist when

 I have reviewed this research and animal husbandry with the student before the 
startI of experimentation.
have reviewed this research and animal husbandry with the student before th
  I will directly supervise the experiment.
I have approved the use and dosages of prescription drugs and/or nutritional supplements.
 I will provide veterinary medical and nursing care in case of illness or emergency. (Fees may apply.)
Printed Name Email/Phone Printed Name Email/Phone
Signature Date of Approval (mm/dd/yy) Signature Date of Approval (mm/dd/yy)

Page
Vertebrate Animal Form (5B)
tebrate animals that is conducted in at a Regulated Research Institution. (IACUC approval required before experimentation. Form must be com
Student’s Name(s)
Title of Project
Title and Protocol Number of IACUC Approved Project
To be completed by Qualified Scientist or Principal Investigator:

1. Species of animals used: Number of animals used:
2. Describe, in detail, the role of the student in this project: animal procedures and related equipment that were involved,
oversight provided and safety precautions employed. (Attach extra pages if necessary.)
3. Was there any weight loss or death of any animal? If yes, attach a letter obtained from the qualified scientist, designated supervisor
or a veterinarian documenting the situation and the results of the investigation.
4. Did the student’s project also involve the use of tissues?

 No
 Yes; complete Forms 6A and 6B
5. What laboratory training, including dates, was provided to the student?
6. Attach a copy of the Regulated Research Institution IACUC Approval. A letter from the Qualified Scientist or Principal
Investigator is not sufficient.
Qualified Scientist/Principal Investigator
Printed Name
Signature Date (mm/dd/yy)

Page
Potentially Hazardous Biological Agents Risk Assessment Form (6A)
microorganisms, rDNA, fresh/frozen tissue (including primary cell lines, human and other primate established cell lines and tissue cultures), b
SRC/IACUC/IBC approval required before experimentation.
Student’s Name(s)
Title of Project
To be completed by the QUALIFIED SCIENTIST/DESIGNATED SUPERVISOR in collaboration with the student researcher(s). All
questions are applicable and must be answered; additional page(s) may be attached.
SECTION 1: PROJECT ASSESSMENT
1. Identify potentially hazardous biological agents to be used in this experiment. Include the source, quantity and the biosafety level risk
group of each microorganism.
2. Describe the site of experimentation including the level of biological containment.
3. Describe the procedures that will be used to minimize risk (personal protective equipment, hood type, etc.).
4. What final biosafety level do you recommend for this project given the risk assessment you conducted?
5. Describe the method of disposal of all cultured materials and other potentially hazardous biological agents.
SECTION 2: TRAINING
1. What training will the student receive for this project?
2. Experience/training of Designated Supervisor as it relates to the student’s area of research (if applicable).
SECTION 3: For ALL CELL LINES, MICROORGANISMS AND TISSUES – To be completed by the QUALIFIED SCIENTIST or
DESIGNATED SUPERVISOR - Check the appropriate box(es) below:
 Experimentation on the microorganisms/cell lines/tissues to be used in this study will NOT be conducted at a Regulated Research Institution, but will be conducted at a
(check one) BSL-1 or BSL-2 laboratory. [This study has been reviewed by the local SRC and the procedures have been approved prior to experimentation.]
 Experimentation on the microorganisms/cell lines/tissues to be used in this study will be conducted at a Regulated Research Institution and was approved by the
appropriate institutional board prior to experimentation; institutional approval forms are attached.
Origin of cell lines: Date of IACUC/IBC approval
 Experimentation on the microorganisms/cell lines/tissues to be used in this study will be conducted at a Regulated Research Institution, which does not require pre-
approval for this type of study. The SRC has seen and approved the research plan and supporting documentation and acknowledges the accuracy of the responses
above.
CERTIFICATION – To be SIGNED by the QUALIFIED SCIENTIST or DESIGNATED SUPERVISOR

The QS/DS has seen this project’s research plan and supporting documentation and acknowledges the accuracy of the information provided above. This study has
been approved as a (check one)  BSL-1/  BSL-2 study, and will be conducted in an appropriate laboratory.
QS/DS Printed Name Signature
Date of review (mm/dd/yy)
SECTION 4: CERTIFICATION – To be completed by the LOCAL or AFFILIATED FAIR SRC

The SRC has seen this project’s research plan and supporting documentation and acknowledges the accuracy of the information provided above.
SRC Printed Name Signature
Date of review (mm/dd/yy)

Page
Human and Vertebrate Animal Tissue Form (6B)
ate established cell lines and tissue cultures), blood, blood products and body fluids. If the research involves living organisms please ensure that the proper huma
Student’s Name(s)
Title of Project
To be completed by Student Researcher(s):
1. What vertebrate animal tissue will be used in this study? Check all that apply.
 Fresh or frozen tissue sample
 Fresh organ or other body part
 Blood
 Body fluids
 Primary cell/tissue cultures
 Human or other primate established cell lines
2. Where will the above tissue(s) be obtained? If using an established cell line include source and catalog number.
3. If the tissue will be obtained from a vertebrate animal study conducted at a research institution attach a copy of the IACUC certification
with the name of the research institution, the title of the study, the IACUC approval number and a of IACUC approval.
To be completed by the Qualified Scientist or Designated Supervisor:

 I verify that the student will work solely with organs, tissues, cultures or cells that will be supplied to him/her by myself or qualified personnel from th
AND/OR
 I certify that the blood, blood products, tissues or body fluids in this project will be handled in accordance with the standards and guidance set forth in
Printed Name Signature Date of Approval (mm/dd/yy)

Title Phone/Email
Institution

Page
Continuation/Research Progression Projects Form (7)
nuation/progression in the same field of study as a previous project. This form must be accompanied by the previous year’s abstr
Student’s Name(s)
To be completed by Student Researcher: List all components of the current project that make it new and different from previous research. The
information must be on the form; use an additional form for previous year and earlier projects.
Components Current Research Project Previous Research Project: Year:

1. Title
2. Change in goal/
purpose/objec-
tive
3. Changes in
methodology
4. Variable studied
5. Additional
changes
Attached are:
 Abstract and Research Plan/Project Summary, Year
I hereby certify that the above information is correct and that the current year Abstract & Certification and project display board properly reflect work done
Student’s Printed Name(s) Signature Date of Signature (mm/dd/yy)

Page
Page
The International Science and Engineering Fair encourages students to tackle challenging scientific
questions and develop the skills needed to solve the problems of tomorrow.
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STEM education and scientific research. Founded in 1921, we are a nonprofit 501(c)(3) membership organization
focused on promoting the understanding and appreciation of science and the vital role it plays in human advancement.
Through its acclaimed science research competitions, including the Regeneron Science Talent Search, the
International Science and Engineering Fair and the Broadcom MASTERS, and its award-winning magazine, Science
News and digital media properties, Science News for Students, the Society is committed to inform, educate and
inspire.
Learn more at:

www.societyforscience.org and
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INTERNATIONAL RULES

FOR PRE-COLLEGE SCIENCE

Roles and Responsibilities of Students and Adults

Human Participants Rules

Vertebrate Animals Rules

Potentially Hazardous Biological Agents Rules

Hazardous Chemicals, Activities or Devices Rules

ISEF RULES WIZARD

COMMON SRC PROBLEMS

THESE RULES ARE APPLICABLE FOR

International Rules: Guidelines for Science and Engineering Fairs 2020–2021,

International Rules: Guidelines for Science and Engineering Fairs 2020–2021,

The Student Researcher(s) Responsibilities:

International Rules: Guidelines for Science and Engineering Fairs 2020–2021,

Certain areas of research conducted in a regulated research institution or an

The ISEF Scientific Review Committee (ISEF SRC)

International Rules: Guidelines for Science and Engineering Fairs 2020–2021,

International Rules: Guidelines for Science and Engineering Fairs 2020–2021,

1. Examples of Greater than Minimal Physical Risk

2. Examples of Greater than Minimal Psychological Risk

International Rules: Guidelines for Science and Engineering Fairs 2020–2021,

Some protocols permitted in a Regulated Research Institution

2. Student researchers are prohibited from performing euthanasia. Euthanasia

3. Research projects that cause more than momentary or slight pain or

4. Research in nutritional deficiency or research involving substances or

5. The following forms are required:

Sources of Information are available as a separate section at the end of the

International Rules: Guidelines for Science and Engineering Fairs 2020–2021,

Students are permitted to do research projects with potentially hazardous

International Rules: Guidelines for Science and Engineering Fairs 2020–2021,

Classification of Biological Agents

erious economic consequences. Projects in the BSL-3 group are prohibited.

ntreatable. Projects in the BSL-4 group are prohibited.

International Rules: Guidelines for Science and Engineering Fairs 2020–2021,

International Rules: Guidelines for Science and Engineering Fairs 2020–2021,

The purchase of a firearm by a minor is generally unlawful. The use of

2. A fully assembled rocket motor, reload kit or propellant modules

2. If the voltage needed in the study is <10 kvolts, a risk assessment

3. A study using 10–25 kvolts must have a risk assessment conducted

A risk assessment (documented on Form 3) must include proper

International Rules: Guidelines for Science and Engineering Fairs 2020–2021,

Engineering and Invention Project Checklist

Hazardous Chemicals, Activities and Devices

Device Testing with Human Participants

Potentially Hazardous Biological Agents

International Rules: Guidelines for Science and Engineering Fairs 2020–2021,

2. General 11. U.S. Nuclear Regulatory Commission

4. Safety and Data Sheets (SDS)

www.ilpi.com/msds/index.html - A listing of numerous sites that have

NFPA (National Fire Protection Association) 704 Standard for guidance

Environmental Protection Agency

6. DEA Controlled Substances

International Rules: Guidelines for Science and Engineering Fairs 2020–2021,

Display & Safety Committee Mission

International Rules: Guidelines for Science and Engineering Fairs 2020–2021,

17. Inadequately insulated apparatus capable of producing dangerous

Please visit our website at student.societyforscience.org/intel-isef-categories-and-subcategories for a full description and

International Rules: Guidelines for Science and Engineering Fairs 2020–2021,

Completing the Abstract