A hexokinase is an enzyme that phosphorylates hexoses (six-carbon sugars), forming hexose phosphate. In most organisms, glucose is the most important substrate of hexokinases, and glucose-6-phosphate is the most important product. Hexokinase can transfer an inorganic phosphate group from ATP to a substrate.
Hexokinases should not be confused with glucokinase, which is a specific isoform of hexokinase. While other hexokinases are capable of phosphorylating several hexoses, glucokinase acts with a 50-fold lower substrate affinity and its only hexose substrate is glucose.
Variation
Genes that encode hexokinase have been discovered in every domain of life, and exist among a variety of species that range from bacteria, yeast, and plants to humans and other vertebrates. They are categorized as actin fold proteins, sharing a common ATP binding site core that is surrounded by more variable sequences which determine substrate affinities and other properties.
Several hexokinase isoforms or isozymes that provide different functions can occur in a single species.
This video describes the difference between glucokinase and hexokinase in details .
published: 25 Jun 2020
What does hexokinase mean?
What does hexokinase mean?
A spoken definition of hexokinase.
Intro Sound:
Typewriter - Tamskp
Licensed under CC:BA 3.0
Outro Music:
Groove Groove - Kevin MacLeod (incompetech.com)
Licensed under CC:BA 3.0
Intro/Outro Photo:
The best days are not planned - Marcus Hansson
Licensed under CC-BY-2.0
Book Image:
Open Book template PSD - DougitDesign
Licensed under CC:BA 3.0
Text derived from:
http://en.wiktionary.org/wiki/hexokinase
Text to Speech powered by TTS-API.COM
published: 27 Aug 2015
Hexokinase Vs Glucokinase | Regulation of Glycolysis | Metabolism | Biochemistry
This video provides a tutorial on important difference between hexokinase and glucokinase enzyme. Hexokinase enzyme has low Km (0.1 mM, high affinity for substrate) and low Vmax) low-capacity enzyme). This allows hexokinase enzyme to convert glucose to glucose-6-phosphate even under the presence of low glucose concentration. On the other hand, glucokinase enzyme Vmax (high-capacity enzyme). This allows glucokinase enzyme to convert glucose to glucose-6-phosphate only in the presence of plenty of tissue glucose concentration, for instance after carbohydrate-rich meal.
The following topics are covered in this lesson,
1. Hexokinase enzyme
2. Glucokinase enzyme
3. Difference between hexokinase and glucokinase enzyme
4. Km of hexokinase enzyme
5. Vmax of hexokinase enzyme
6. Km of glucokina...
published: 26 Mar 2022
Using Molecular Visualization Software, Pymol, to understand metabolic enzymes: Hexokinase
Glycolysis is the conversion of glucose into pyruvate to generate energy in the form of adenine triphosphate (ATP). The 1st step of glycolysis is the conversion of glucose to glucose-6-phosphate and by transferring a phosphate group from ATP and forming ADP. This reaction requires the enzyme hexokinase, which is found in all multicellular organisms. In this video, we will show how the overall structure of hexokinase changes upon binding its substrate glucose and show how the shape of the activate site accommodates and positions both the ATP and glucose molecules for a chemical reaction to occur.
published: 26 Apr 2021
Hexokinase: Ein Kurzfilm der Biotechnologie Studierenden (BT 15)
Inspirationen eines interdisziplinären Austauschen zwischen den Studiengängen der Biotechnologie und der Lebensmitteltechnologie im Rahmen der Biochemie Vorlesungsreihe: Die Studierenden der LM15 haben ein Klassenzimmer in einen Rundgang durch den Energiestoffwechsel verwandelt. Die Studierenden der BT15 entführten in die Welt der Proteine mit Postern, Präsentationen und diesem Kurzfilm.
published: 19 Dec 2016
3. Hexokinase and Glucokinase, inhibition of Enolase by fluoride ions, continuation of glycolysis
Carbohydrate metabolism Part 2
For medical and dental students
For NEET preparation
Hexokinase
act on all hexoses
High affinity
low Km value
active in all tissue
not inducible
inhibition by Glu 6 phosphate
active even in low glucose concentration
Glucokinase
act only on glucose
Low affinity
high Km value
active in liver and pancreas
inducible
not inhibited by Glu 6 phosphate
active only when glucose concentration is more than 100 mg/dl
published: 06 Dec 2018
Regulation of hexokinase 4
Hexokinase iv regulation mechanism.
http://shomusbiology.weebly.com/
Download the study materials here-
http://shomusbiology.weebly.com/bio-materials.html
Mammalian hexokinase IV, also referred to as glucokinase, differs from other hexokinases in kinetics and functions.
The location of the phosphorylation on a subcellular level occurs when glucokinase translocates between the cytoplasm and nucleus of liver cells. Glucokinase can only phosphorylate glucose if the concentration of this substrate is high enough; its Km for glucose is 100 times higher than that of hexokinases I, II, and III.
Hexokinase IV is monomeric, about 50kD, displays positive cooperativity with glucose, and is not allosterically inhibited by its product, glucose-6-phosphate.
Hexokinase IV is present in the liver, panc...
What does hexokinase mean?
A spoken definition of hexokinase.
Intro Sound:
Typewriter - Tamskp
Licensed under CC:BA 3.0
Outro Music:
Groove Groove - K...
What does hexokinase mean?
A spoken definition of hexokinase.
Intro Sound:
Typewriter - Tamskp
Licensed under CC:BA 3.0
Outro Music:
Groove Groove - Kevin MacLeod (incompetech.com)
Licensed under CC:BA 3.0
Intro/Outro Photo:
The best days are not planned - Marcus Hansson
Licensed under CC-BY-2.0
Book Image:
Open Book template PSD - DougitDesign
Licensed under CC:BA 3.0
Text derived from:
http://en.wiktionary.org/wiki/hexokinase
Text to Speech powered by TTS-API.COM
What does hexokinase mean?
A spoken definition of hexokinase.
Intro Sound:
Typewriter - Tamskp
Licensed under CC:BA 3.0
Outro Music:
Groove Groove - Kevin MacLeod (incompetech.com)
Licensed under CC:BA 3.0
Intro/Outro Photo:
The best days are not planned - Marcus Hansson
Licensed under CC-BY-2.0
Book Image:
Open Book template PSD - DougitDesign
Licensed under CC:BA 3.0
Text derived from:
http://en.wiktionary.org/wiki/hexokinase
Text to Speech powered by TTS-API.COM
This video provides a tutorial on important difference between hexokinase and glucokinase enzyme. Hexokinase enzyme has low Km (0.1 mM, high affinity for substr...
This video provides a tutorial on important difference between hexokinase and glucokinase enzyme. Hexokinase enzyme has low Km (0.1 mM, high affinity for substrate) and low Vmax) low-capacity enzyme). This allows hexokinase enzyme to convert glucose to glucose-6-phosphate even under the presence of low glucose concentration. On the other hand, glucokinase enzyme Vmax (high-capacity enzyme). This allows glucokinase enzyme to convert glucose to glucose-6-phosphate only in the presence of plenty of tissue glucose concentration, for instance after carbohydrate-rich meal.
The following topics are covered in this lesson,
1. Hexokinase enzyme
2. Glucokinase enzyme
3. Difference between hexokinase and glucokinase enzyme
4. Km of hexokinase enzyme
5. Vmax of hexokinase enzyme
6. Km of glucokinase enzyme
7. Vmax of glucokinase enzyme
8. Importance of glucokinase and hexokinase in regulating blood glucose level
9. Glucokinase Vs hexokinase affinity for glucose
10. Glucokinase Vs hexokinase function
11. Glucokinase Vs hexokinase regulation
This video provides a tutorial on important difference between hexokinase and glucokinase enzyme. Hexokinase enzyme has low Km (0.1 mM, high affinity for substrate) and low Vmax) low-capacity enzyme). This allows hexokinase enzyme to convert glucose to glucose-6-phosphate even under the presence of low glucose concentration. On the other hand, glucokinase enzyme Vmax (high-capacity enzyme). This allows glucokinase enzyme to convert glucose to glucose-6-phosphate only in the presence of plenty of tissue glucose concentration, for instance after carbohydrate-rich meal.
The following topics are covered in this lesson,
1. Hexokinase enzyme
2. Glucokinase enzyme
3. Difference between hexokinase and glucokinase enzyme
4. Km of hexokinase enzyme
5. Vmax of hexokinase enzyme
6. Km of glucokinase enzyme
7. Vmax of glucokinase enzyme
8. Importance of glucokinase and hexokinase in regulating blood glucose level
9. Glucokinase Vs hexokinase affinity for glucose
10. Glucokinase Vs hexokinase function
11. Glucokinase Vs hexokinase regulation
Glycolysis is the conversion of glucose into pyruvate to generate energy in the form of adenine triphosphate (ATP). The 1st step of glycolysis is the conversi...
Glycolysis is the conversion of glucose into pyruvate to generate energy in the form of adenine triphosphate (ATP). The 1st step of glycolysis is the conversion of glucose to glucose-6-phosphate and by transferring a phosphate group from ATP and forming ADP. This reaction requires the enzyme hexokinase, which is found in all multicellular organisms. In this video, we will show how the overall structure of hexokinase changes upon binding its substrate glucose and show how the shape of the activate site accommodates and positions both the ATP and glucose molecules for a chemical reaction to occur.
Glycolysis is the conversion of glucose into pyruvate to generate energy in the form of adenine triphosphate (ATP). The 1st step of glycolysis is the conversion of glucose to glucose-6-phosphate and by transferring a phosphate group from ATP and forming ADP. This reaction requires the enzyme hexokinase, which is found in all multicellular organisms. In this video, we will show how the overall structure of hexokinase changes upon binding its substrate glucose and show how the shape of the activate site accommodates and positions both the ATP and glucose molecules for a chemical reaction to occur.
Inspirationen eines interdisziplinären Austauschen zwischen den Studiengängen der Biotechnologie und der Lebensmitteltechnologie im Rahmen der Biochemie Vorlesu...
Inspirationen eines interdisziplinären Austauschen zwischen den Studiengängen der Biotechnologie und der Lebensmitteltechnologie im Rahmen der Biochemie Vorlesungsreihe: Die Studierenden der LM15 haben ein Klassenzimmer in einen Rundgang durch den Energiestoffwechsel verwandelt. Die Studierenden der BT15 entführten in die Welt der Proteine mit Postern, Präsentationen und diesem Kurzfilm.
Inspirationen eines interdisziplinären Austauschen zwischen den Studiengängen der Biotechnologie und der Lebensmitteltechnologie im Rahmen der Biochemie Vorlesungsreihe: Die Studierenden der LM15 haben ein Klassenzimmer in einen Rundgang durch den Energiestoffwechsel verwandelt. Die Studierenden der BT15 entführten in die Welt der Proteine mit Postern, Präsentationen und diesem Kurzfilm.
Carbohydrate metabolism Part 2
For medical and dental students
For NEET preparation
Hexokinase
act on all hexoses
High affinity
low Km value
active in all tiss...
Carbohydrate metabolism Part 2
For medical and dental students
For NEET preparation
Hexokinase
act on all hexoses
High affinity
low Km value
active in all tissue
not inducible
inhibition by Glu 6 phosphate
active even in low glucose concentration
Glucokinase
act only on glucose
Low affinity
high Km value
active in liver and pancreas
inducible
not inhibited by Glu 6 phosphate
active only when glucose concentration is more than 100 mg/dl
Carbohydrate metabolism Part 2
For medical and dental students
For NEET preparation
Hexokinase
act on all hexoses
High affinity
low Km value
active in all tissue
not inducible
inhibition by Glu 6 phosphate
active even in low glucose concentration
Glucokinase
act only on glucose
Low affinity
high Km value
active in liver and pancreas
inducible
not inhibited by Glu 6 phosphate
active only when glucose concentration is more than 100 mg/dl
Hexokinase iv regulation mechanism.
http://shomusbiology.weebly.com/
Download the study materials here-
http://shomusbiology.weebly.com/bio-materials.html
Mamma...
What does hexokinase mean?
A spoken definition of hexokinase.
Intro Sound:
Typewriter - Tamskp
Licensed under CC:BA 3.0
Outro Music:
Groove Groove - Kevin MacLeod (incompetech.com)
Licensed under CC:BA 3.0
Intro/Outro Photo:
The best days are not planned - Marcus Hansson
Licensed under CC-BY-2.0
Book Image:
Open Book template PSD - DougitDesign
Licensed under CC:BA 3.0
Text derived from:
http://en.wiktionary.org/wiki/hexokinase
Text to Speech powered by TTS-API.COM
This video provides a tutorial on important difference between hexokinase and glucokinase enzyme. Hexokinase enzyme has low Km (0.1 mM, high affinity for substrate) and low Vmax) low-capacity enzyme). This allows hexokinase enzyme to convert glucose to glucose-6-phosphate even under the presence of low glucose concentration. On the other hand, glucokinase enzyme Vmax (high-capacity enzyme). This allows glucokinase enzyme to convert glucose to glucose-6-phosphate only in the presence of plenty of tissue glucose concentration, for instance after carbohydrate-rich meal.
The following topics are covered in this lesson,
1. Hexokinase enzyme
2. Glucokinase enzyme
3. Difference between hexokinase and glucokinase enzyme
4. Km of hexokinase enzyme
5. Vmax of hexokinase enzyme
6. Km of glucokinase enzyme
7. Vmax of glucokinase enzyme
8. Importance of glucokinase and hexokinase in regulating blood glucose level
9. Glucokinase Vs hexokinase affinity for glucose
10. Glucokinase Vs hexokinase function
11. Glucokinase Vs hexokinase regulation
Glycolysis is the conversion of glucose into pyruvate to generate energy in the form of adenine triphosphate (ATP). The 1st step of glycolysis is the conversion of glucose to glucose-6-phosphate and by transferring a phosphate group from ATP and forming ADP. This reaction requires the enzyme hexokinase, which is found in all multicellular organisms. In this video, we will show how the overall structure of hexokinase changes upon binding its substrate glucose and show how the shape of the activate site accommodates and positions both the ATP and glucose molecules for a chemical reaction to occur.
Inspirationen eines interdisziplinären Austauschen zwischen den Studiengängen der Biotechnologie und der Lebensmitteltechnologie im Rahmen der Biochemie Vorlesungsreihe: Die Studierenden der LM15 haben ein Klassenzimmer in einen Rundgang durch den Energiestoffwechsel verwandelt. Die Studierenden der BT15 entführten in die Welt der Proteine mit Postern, Präsentationen und diesem Kurzfilm.
Carbohydrate metabolism Part 2
For medical and dental students
For NEET preparation
Hexokinase
act on all hexoses
High affinity
low Km value
active in all tissue
not inducible
inhibition by Glu 6 phosphate
active even in low glucose concentration
Glucokinase
act only on glucose
Low affinity
high Km value
active in liver and pancreas
inducible
not inhibited by Glu 6 phosphate
active only when glucose concentration is more than 100 mg/dl
A hexokinase is an enzyme that phosphorylates hexoses (six-carbon sugars), forming hexose phosphate. In most organisms, glucose is the most important substrate of hexokinases, and glucose-6-phosphate is the most important product. Hexokinase can transfer an inorganic phosphate group from ATP to a substrate.
Hexokinases should not be confused with glucokinase, which is a specific isoform of hexokinase. While other hexokinases are capable of phosphorylating several hexoses, glucokinase acts with a 50-fold lower substrate affinity and its only hexose substrate is glucose.
Variation
Genes that encode hexokinase have been discovered in every domain of life, and exist among a variety of species that range from bacteria, yeast, and plants to humans and other vertebrates. They are categorized as actin fold proteins, sharing a common ATP binding site core that is surrounded by more variable sequences which determine substrate affinities and other properties.
Several hexokinase isoforms or isozymes that provide different functions can occur in a single species.