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Electrophoresis

Electrophoresis is the motion of dispersed particles relative to a fluid under the influence of a spatially uniform electric field. This electrokinetic phenomenon was observed for the first time in 1807 by Ferdinand Frederic Reuss (Moscow State University), who noticed that the application of a constant electric field caused clay particles dispersed in water to migrate. It is ultimately caused by the presence of a charged interface between the particle surface and the surrounding fluid. It is the basis for a number of analytical techniques used in biochemistry for separating molecules by size, charge, or binding affinity.

Electrophoresis of positively charged particles (cations) is called cataphoresis, while electrophoresis of negatively charged particles (anions) is called anaphoresis. Electrophoresis is a technique used in laboratories in order to separate macromolecules based on size. The technique applies a negative charge so proteins move towards a positive charge. This is used for both DNA and RNA analysis. Polyacrylamide gel electrophoresis (PAGE) has a clearer resolution than agarose and is more suitable for quantitative analysis. In this technique DNA foot-printing can identify how proteins bind to DNA. It can be used to separate proteins by size, density and purity. It can also be used for plasmid analysis, which develops our understanding of bacteria becoming resistant to antibiotics.

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This page contains text from Wikipedia, the Free Encyclopedia - https://wn.com/Electrophoresis

Electrophoresis (journal)

Electrophoresis is a peer-reviewed scientific journal covering all aspects of electrophoresis, including new or improved analytical and preparative methods, development of theory, and innovative applications of electrophoretic methods in the study of proteins, nucleic acids, and other compounds.

Abstracting and indexing

The journal is abstracted and indexed in:

Advanced Polymer Abstracts

AGRICOLA

Animal Breeding Abstracts

Biochemistry & Biophysics Citation Index

Biological Abstracts

BIOSIS Previews

CAB Abstracts

CAB HEALTH

CAB Direct

Cambridge Scientific Abstracts

Civil Engineering Abstracts

Current Contents/Life Sciences

EMBASE

Global Health

Index Medicus/MEDLINE/PubMed

Inspec

METADEX

Science Citation Index

Scopus

According to the Journal Citation Reports, the journal has a 2014 impact factor of 3.028, ranking it 14th out of 74 journals in the category "Chemistry, Analytical" and 24th out of 79 in the category "Biochemical Research Methods".

References

External links

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This page contains text from Wikipedia, the Free Encyclopedia - https://wn.com/Electrophoresis_(journal)

Electrophoresis (disambiguation)

Electrophoresis is the motion of dispersed particles relative to a fluid under the influence of a spatially uniform electric field.

Electrophoresis can also refer to:

Interface and Colloid Science

Dielectrophoresis, similar motion in a space non-uniform electric field

Microelectrophoresis, a method of studying electrophoresis of various dispersed particles using optical microscopy

Electrophoretic light scattering, a method for measuring electrophoretic mobility based on dynamic light scattering

Molecular Biology and Biochemistry

Affinity electrophoresis, used to separate and characterize biomolecules on basis of their molecular characteristics through binding to another biomolecule

Capillary electrophoresis, commonly used to separate biomolecules by their charge and frictional forces

Gel electrophoresis, a technique used by scientists to separate molecules based on physical characteristics such as size, shape, or isoelectric point

• DNA electrophoresis, a specific type of gel electrophoresis used to analyse DNA
• Protein electrophoresis, a specific type of gel electrophoresis used to analyse proteins
• Two-dimensional gel electrophoresis, a specific type of gel electrophoresis commonly used to analyse proteins which involves two separation mechanisms to separate molecules
• SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, commonly used to analyse proteins

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This page contains text from Wikipedia, the Free Encyclopedia - https://wn.com/Electrophoresis_(disambiguation)

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