Homocitrat sintaza
| Homocitrat sintaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| |||||||||
| Homocitrat sintaza homodimer, Schizosaccharomyces pombe | |||||||||
| Identifikatori | |||||||||
| EC broj | 2.3.3.14 | ||||||||
| CAS broj | 9075-60-9 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
Homocitrat sintaza (EC 2.3.3.14, 2-hidroksibutan-1,2,4-trikarboksilat 2-oksoglutarat-lijaza (KoA-acetilacija), acetil-koenzim A:2-ketoglutarat C-acetil transferaza, homocitrat sintetaza, HCS) je enzim sa sistematskim imenom acetil-KoA:2-oksoglutarat C-acetiltransferaza (tioestarska hidroliza, formiranje karboksimetila).[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju
- acetil-KoA + H2O + 2-oksoglutarat (2R)-2-hidroksibutan-1,2,4-trikarboksilat + KoA
Ovaj enzim učestvuje u alfa-aminoadipatnom putu sinteze lizina, zajedno sa EC 4.2.1.36, homoakonitatnom hidratazom.
- ↑ Strassman, M. and Ceci, L.N. (1964). „Enzymatic formation of homocitric acid, an intermediate in lysine biosynthesis”. Biochem. Biophys. Res. Commun. 14: 262-267. PMID 5836514.
- ↑ Wulandari, A.P., Miyazaki, J., Kobashi, N., Nishiyama, M., Hoshino, T. and Yamane, H. (2002). „Characterization of bacterial homocitrate synthase involved in lysine biosynthesis”. FEBS Lett. 522: 35-40. PMID 12095615.
- ↑ Andi, B., West, A.H. and Cook, P.F. (2004). „Kinetic mechanism of histidine-tagged homocitrate synthase from Saccharomyces cerevisiae”. Biochemistry 43: 11790-11795. PMID 15362863.
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