Endothelial Dysfunction Markers in Ovarian Cancer: VTE Risk and Tumour Prognostic Outcomes
Arrhythmias Following Patent Foramen Ovale Closure: An Unsolved Enigma
Potential of Stem-Cell-Induced Peripheral Nerve Regeneration: From Animal Models to Clinical Trials
Anderson–Fabry Disease: Focus on Ophthalmological Implications
Prevalence and Antibiotic Resistance of Streptococcus agalactiae in Women of Childbearing Age Presenting Urinary Tract Infections from Western Romania

Journal Description

Life

Life is an international, peer-reviewed, open access journal of scientific studies related to fundamental themes in life sciences, from basic to applied research, published monthly online by MDPI. The Astrobiology Society of Britain (ASB) and Spanish Association for Cancer Research (ASEICA) are affiliated with Life and their members receive a discount on the article processing charges.

Open Access— free for readers, with article processing charges (APC) paid by authors or their institutions.
High Visibility: indexed within Scopus, SCIE (Web of Science), PubMed, PMC, CAPlus / SciFinder, AGRIS, and other databases.
Journal Rank: JCR - Q1 (Biology) / CiteScore - Q1 (Paleontology)
Rapid Publication: manuscripts are peer-reviewed and a first decision is provided to authors approximately 17.8 days after submission; acceptance to publication is undertaken in 2.5 days (median values for papers published in this journal in the second half of 2024).
Recognition of Reviewers: reviewers who provide timely, thorough peer-review reports receive vouchers entitling them to a discount on the APC of their next publication in any MDPI journal, in appreciation of the work done.
Testimonials: See what our editors and authors say about Life.
Companion journals for Life include: Physiologia and Hydrobiology.

Impact Factor: 3.2 (2023); 5-Year Impact Factor: 3.1 (2023)

Imprint Information Journal Flyer Open Access ISSN: 2075-1729

Latest Articles

19 pages, 1555 KiB

Open AccessReview

Left Atrial Strain in Patients with Chronic Heart Failure with Preserved Ejection Fraction: A Narrative Review

by Dana Emilia Man, Alexandru Catalin Motofelea, Valentina Buda, Dana Emilia Velimirovici, Olivia Bodea, Daniel Marius Duda-Seiman, Constantin Tudor Luca and Simona-Ruxanda Dragan

Life 2025, 15(2), 313; https://doi.org/10.3390/life15020313 - 17 Feb 2025

Abstract

Background: Heart failure with preserved ejection fraction (HFpEF) represents a significant portion of heart failure cases, but diagnosis is challenging due to its diverse presentation and the limitations of traditional echocardiographic parameters. Left atrial (LA) strain provides valuable insights into LA function and is increasingly used to evaluate cardiac function, including left ventricular (LV) diastolic function. LA strain, particularly reservoir strain, is considered a reliable indicator of LV diastolic function and can be used to grade diastolic function and estimate LV filling pressure. Unlike traditional LA measurements, LA strain offers detailed insights into LA function, conduit, and booster-pump phases, making it crucial for evaluating both structural and functional cardiac performance, especially in HFpEF. HFpEF diagnosis currently relies on a combination of echocardiographic parameters, clinical symptoms, and natriuretic peptide levels, encompassing various pathophysiological entities and complicating standardized management. Precise characterization of cardiac pathologies in HFpEF patients is essential. This review assesses global longitudinal strain (GLS) and left atrial strain (LAS) as echocardiographic biomarkers for diagnosing and characterizing HFpEF. Strain imaging, particularly speckle tracking echocardiography, offers a refined assessment of myocardial deformation, providing detailed insights into left heart function beyond traditional measures. Normal ranges for GLS and LAS are discussed, acknowledging demographic and technical influences. Clinical studies confirm the prognostic value of GLS and LAS in HFpEF, especially for predicting cardiovascular outcomes and distinguishing HFpEF from other dyspnea causes. However, variability in strain measurements and false-negative risks necessitate cautious clinical interpretation. The HFA-PEFF scoring system includes these biomarkers but does not fully cover the HFpEF pathology spectrum. Combining GLS and LAS shows promise in defining HFpEF phenogroups, potentially guiding individualized treatments. Global longitudinal strain (GLS) and left atrial strain (LAS) are central to non-invasive HFpEF diagnosis and stratification, with potential for more tailored therapies. Integration of these biomarkers into standard diagnostic practice requires an organized approach, and future guidelines should recommend their combined use for comprehensive HFpEF assessment. Full article

(This article belongs to the Special Issue Advancements in Heart Failure Research)

12 pages, 893 KiB

Open AccessArticle

Tailored Predictive Indicators for Weaning Success from High-Flow Nasal Cannula in Postoperative Hypoxemic Patients

by Yuh-Chyn Tsai, Shih-Feng Liu, Hui-Chuan Chang, Ching-Min Huang, Wan-Chun Hsieh, Chin-Ling Li, Ting-Lung Lin and Ho-Chang Kuo

Life 2025, 15(2), 312; https://doi.org/10.3390/life15020312 - 17 Feb 2025

Abstract

The use of high-flow nasal cannula (HFNC) as an oxygen therapy post-extubation has demonstrated varying success rates across different surgical populations. This study aimed to identify the predictive factors influencing HFNC weaning outcomes in patients with postoperative extubation hypoxemia. We conducted a retrospective analysis of patients in a surgical intensive care unit, categorized into three major postoperative groups: cardiothoracic surgery, upper abdominal surgery, and other surgeries. Our analysis examined pre-extubation weaning profiles, vital signs before and after HFNC initiation, and changes in physiological parameters during HFNC use. A total of 90 patients were included, divided into two groups based on HFNC weaning success or failure. Key parameters analyzed included maximal inspiratory pressure (MIP), PaO₂/FiO₂ (P/F) ratio, vital signs, SpO₂ levels, respiratory rate (RR), heart rate (HR), respiratory rate–oxygenation (ROX) index, and HFNC duration. The findings revealed that cardiothoracic and upper abdominal groups showed significantly higher HFNC weaning success rates (73.3% and 70.6%) compared to the other surgeries group (34.6%) (p = 0.004). Critical predictors of successful weaning included pre-HFNC SpO₂, P/F ratio, and changes in the ROX index, particularly in upper abdominal and other surgeries groups. In cardiothoracic surgery patients, higher maximal inspiratory pressure (MIP) (p = 0.031) was associated with improved outcomes, while prolonged HFNC use correlated with weaning success in this group (p = 0.047). These findings underscore the necessity of tailoring HFNC strategies to surgical characteristics and individual patient profiles. For cardiothoracic surgery patients, pre-extubation MIP, post-extubation RR, ΔROX, and ΔHR were identified as key predictive factors. In upper abdominal surgery, pre-extubation P/F ratio, post-extubation SpO₂, and ΔROX played crucial roles. For patients undergoing other types of surgeries, pre-extubation P/F ratio and ΔROX remained the most reliable predictors of HFNC weaning success. Full article

(This article belongs to the Special Issue Advancements in Postoperative Management of Patients After Surgery)

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10 pages, 214 KiB

Open AccessArticle

Multistrain Probiotics and Telomere Length in Type 2 Diabetes: A 24-Week Randomized Controlled Trial

by Venkata Chaithanya, Janardanan Kumar, Kakithakara Vajravelu Leela, Habeeb Ali Baig, Mohamed Soliman, Awwad Alenezy and Naglaa M. Shalaby

Life 2025, 15(2), 311; https://doi.org/10.3390/life15020311 - 17 Feb 2025

Abstract

Background: This 24-week randomized controlled trial aimed to evaluate the impact of multistrain probiotic supplementation on telomere length in patients with type 2 diabetes (T2DM). The study also assessed secondary outcomes including high-sensitivity C-reactive protein (hs-CRP) and glycated hemoglobin (HbA1c). Methods: A total of 124 participants with T2DM were randomly assigned to either a probiotic group (n = 62) or a placebo group (n = 62). Participants in the probiotic group consumed a supplement containing fourteen live microbial strains, including Lactobacillus plantarum, L. fermentum, L. acidophilus, L. casei, L. rhamnosus, L. reuteri, L. salivarius, L. paracasei, L. gasseri, Bifidobacterium bifidum, B. lactis, B. breve, Streptococcus thermophilus, and Saccharomyces boulardii, with each strain providing 2.148 billion CFUs per capsule, totaling 30 billion CFUs. The placebo group received vitamin B12 capsules without probiotics. The primary outcome was telomere length, and secondary outcomes included hs-CRP and HbA1c levels. Data were analyzed using intention-to-treat (ITT) and per-protocol (PP) methods. Results: The probiotic group exhibited a statistically significant decrease in telomere shortening compared to the placebo group (p < 0.001). The hs-CRP levels decreased more significantly in the probiotic group (p < 0.001), suggesting potential anti-inflammatory effects. The HbA1c levels improved in the probiotic group, with a reduction of 0.44% (p = 0.004). An age-stratified analysis revealed more substantial improvements in the 30–49 years cohort, which showed greater reductions in telomere shortening, inflammatory markers, and metabolic indicators compared to the 50–69 years group. Conclusions: Multistrain probiotic supplementation shows potential benefits in reducing telomere shortening and improving glycemic control. However, further long-term studies are needed to understand the underlying mechanisms and therapeutic implications of probiotics in T2DM. Trial registration: This trial was registered at the Clinical Trials Registry-India (CTRI/2023/07/055647). Full article

(This article belongs to the Section Medical Research)

18 pages, 1309 KiB

Open AccessArticle

Accumulation Potential of Lead and Cadmium Metals in Maize (Zea mays L.) and Effects on Physiological-Morphological Characteristics

by Ümit Elik and Zeynep Gül

Life 2025, 15(2), 310; https://doi.org/10.3390/life15020310 - 17 Feb 2025

Abstract

Phytoremediation stands at the forefront of modern environmental science, offering an innovative and cost-effective solution for the remediation of heavy-metal-contaminated soils through the natural capabilities of plants. This study aims to investigate the effects of lead (Pb) and cadmium (Cd) metals on plant growth (e.g., seedling height, stem diameter, fresh and dry weight), physiological properties (e.g., tissue relative water content, tissue electrical conductivity), and biochemical parameters (e.g., chlorophyll content, superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) enzyme activities) of maize compared to the control group under greenhouse conditions at the Atatürk University Plant Production Application and Research Center. The results show that plant height decreased by 20% in the lead (Pb3000) application and by 42% in the cadmium (Cd300) application compared to the control group. The highest Pb dose (Pb3000) caused a 15% weight loss compared to the control, while the highest Cd dose (Cd300) caused a weight loss of 63%. The accumulation rates of heavy metals in soil, roots, and aboveground parts of plants indicated that maize absorbed and accumulated more Cd compared to Pb. Full article

(This article belongs to the Special Issue Plant Functional Genomics and Breeding)

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19 pages, 733 KiB

Open AccessSystematic Review

The Role of Urinary Microbiome Analysis in the Diagnostic Approach and Management of Urinary Incontinence: A Systematic Review

by Pouyan Shaker, Zohreh Roshani, Ehsan Timajchi, Zahra Sharifi, Sarah Nikzadfar Goli, Behrooz Broumand and Mohammad Ali Shafiee

Life 2025, 15(2), 309; https://doi.org/10.3390/life15020309 - 17 Feb 2025

Abstract

Urinary incontinence (UI) is a significant global health issue that impacts mainly middle-aged women, severely affecting their quality of life. Emerging research highlights the urinary microbiome’s complex role in the etiology and management of UI, with microbial dysbiosis potentially influencing symptom severity and treatment outcomes. This systematic review aimed to evaluate the current evidence on the urinary microbiome’s role in diagnosing and managing UI, focusing on variations in microbial composition across UI subtypes. We identified 21 studies, mostly employing 16S rRNA sequencing to characterize urinary microbiota and their associations with various UI subtypes, including urgency urinary incontinence (UUI), overactive bladder (OAB), and stress urinary incontinence (SUI). The findings revealed distinct microbial patterns, such as reduced Lactobacillus levels and increased Gardnerella prevalence, particularly in UUI. Altered microbiome profiles correlated with symptom severity, with reduced Lactobacilli suggesting a protective role in maintaining urinary health. Specific microbial species, including Actinotignum schaalii and Aerococcus urinae, emerged as potential biomarkers for UI diagnosis. Despite promising findings, limitations such as small sample sizes, variability in microbiome profiling methods, and insufficient causal evidence underscore the need for further research. Full article

(This article belongs to the Special Issue Urinary Microbiome and Genitourinary System Disorders: 2nd Edition)

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16 pages, 4508 KiB

Open AccessArticle

Effect of Patient Age on Platelet-Rich Plasma (PRP) and Fibrin Treatments for Skin Density and Thickness: A Single-Center Ultrasound Study

by Lidia Majewska, Jacek Kijowski and Karolina Dorosz

Life 2025, 15(2), 308; https://doi.org/10.3390/life15020308 - 17 Feb 2025

Abstract

Objective: This study aimed to establish a reproducible protocol for obtaining four plasma fractions (autologous platelet-rich plasma (C-PRP, PRP LCC) and platelet-rich fibrin (I-PRF, F-PRF)) from a single blood draw and to evaluate their effectiveness in enhancing skin density and thickness in facial aesthetic treatments across different age groups. Methodology: Twenty participants aged 30–60 years received three treatments at 4–6 week intervals, with C-PRP, PRP LCC, I-PRF, and F-PRF injections in targeted facial areas. Ultrasound measurements provided an objective method to assess the outcomes, and statistical analysis evaluated the changes in skin density and thickness. A comprehensive literature review contextualized our findings within the broader scientific discourse on PRP and PRF applications in aesthetic medicine. Results: The protocol successfully yielded four distinct plasma fractions from a single blood draw. Ultrasound and statistical analyses demonstrated significant improvements in skin density and thickness, particularly in the lower eyelid area. These improvements were consistent across all the age groups, suggesting that the therapy’s effectiveness is independent of age. The findings also align with previous research, underscoring PRP’s and PRF’s roles in stimulating fibroblast activity, promoting collagen synthesis, and enhancing skin quality. Full article

(This article belongs to the Section Medical Research)

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12 pages, 1474 KiB

Open AccessSystematic Review

Efficacy of Dupilumab in the Treatment of Eosinophilic Esophagitis: A Systematic Review and Network Meta-Analysis of Randomized Controlled Trials

by Szu-Hung Chu, Jeng-Jung Chen, Chung-Chu Chen, Wei-Te Lei, Chi-Hone Lien, Shung-Long Weng, Chun-Yan Yeung, Lawrence Yu-Ming Liu, Yu-Lin Tai, Ya-Ning Huang and Chien-Yu Lin

Life 2025, 15(2), 307; https://doi.org/10.3390/life15020307 - 17 Feb 2025

Abstract

Eosinophilic esophagitis (EoE) is a chronic, immune-mediated disorder of the esophagus with rising prevalence. Dupilumab (DUPI), a monoclonal antibody that targets the interleukin-4 receptor α, has shown promise as a treatment option. We conducted a systematic review and network meta-analysis of randomized controlled trials searching the PubMed/Medline database, the Cochrane Database of Systematic Reviews, the Cochrane Central Register of Controlled Trials (CENTRAL), and the medRxiv preprint server up to 31 July 2024, assessing DUPI’s efficacy and optimal dosing in the treatment of EoE. Finally, three randomized-controlled trials comprising 470 participants, including 102 children under 12 years of age, were included in the qualitative synthesis. Both high-exposure (HE-DUPI, 300 mg weekly) and low-exposure (LE-DUPI, 300 mg biweekly) regimens achieved significant histologic remission relative to placebo (OR = 26.88, 95% CI 11.98–60.29 for LE-DUPI; OR = 29.15, 95% CI 13.68–62.12 for HE-DUPI). Although overall adverse events were comparable between groups, HE-DUPI was associated with a notable increase in serious adverse events. These findings suggest that DUPI is effective in promoting histologic remission in EoE, with LE-DUPI emerging as a preferred option for balancing efficacy and safety. This study highlights the efficacy and safety profiles of different dosing regimens and pediatric groups. Further studies are warranted to explore long-term outcomes and identify patient subgroups that may derive the greatest benefit from DUPI therapy. Full article

(This article belongs to the Special Issue Gastrointestinal Health: Clinical Research and Therapeutic Innovations)

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20 pages, 998 KiB

Open AccessArticle

Health Benefits of Montmorency Tart Cherry Juice Supplementation in Adults with Mild to Moderate Ulcerative Colitis; A Placebo Randomized Controlled Trial

by Jonathan Sinclair, Graham McLaughlin, Robert Allan, Johanne Brooks-Warburton, Charlotte Lawson, Shan Goh, Terun Desai and Lindsay Bottoms

Life 2025, 15(2), 306; https://doi.org/10.3390/life15020306 - 17 Feb 2025

Abstract

Aims: Ulcerative colitis (UC) significantly impacts individuals’ self-perception, body image, and overall quality of life, while also imposing considerable economic costs. These challenges highlight the necessity for complementary therapeutic strategies with reduced adverse effects to support conventional pharmacological treatments. Among natural interventions, Montmorency tart cherries, noted for their high anthocyanin content have emerged as a natural anti-inflammatory agent for UC. The current trial aimed to investigate the effects of Montmorency tart cherries compared to placebo in patients with mild to moderate UC. Materials and methods: Thirty-five patients with UC were randomly assigned to receive either placebo or Montmorency tart cherry juice, of which they drank 60 mL per day for 6 weeks. The primary outcomes and health-related quality of life, measured via the Inflammatory Bowel Disease Quality of Life Questionnaire (IBDQ), and the secondary measures, including other health-related questionnaires, blood biomarkers, and faecal samples, were measured before and after the intervention. Linear mixed-effects models were adopted to contrast the changes from baseline to 6 weeks between trial arms. Effect sizes were calculated using Cohen’s d. Results: There were significantly greater improvements in the IBDQ (22.61 (95% CI = 5.24 to 39.99) d = 0.90) and simple clinical colitis activity index (−3.98 (95% CI = −6.69 to –1.28) d = −1.01) in the tart cherry trial arm compared to placebo. In addition, reductions in faecal calprotectin levels were significantly greater in the tart cherry trial arm compared to placebo (−136.17 µg/g (95% CI = −258.06 to –4.28) d = −1.14). Loss to follow-up (N = 1) and adverse events (N = 1) were low and compliance was very high in the tart cherry (95.8%) trial arm. Conclusions: Given the profoundly negative effects of UC on health-related quality of life and its fiscal implications for global healthcare systems, this trial indicates that twice-daily tart cherry supplementation can improve IBD-related quality of life as well as the severity of symptoms and therefore may be important in the management of UC. Full article

(This article belongs to the Collection Clinical Trials)

18 pages, 5562 KiB

Open AccessReview

Impact of Altitude Training on Athletes’ Aerobic Capacity: A Systematic Review and Meta-Analysis

by Lin Deng, Yuhang Liu, Baili Chen, Jiawan Hou, Ao Liu and Xiaoyi Yuan

Life 2025, 15(2), 305; https://doi.org/10.3390/life15020305 - 17 Feb 2025

Abstract

Purpose: This study systematically evaluated the effects of altitude training on athletes’ aerobic capacity, focusing on optimal training modalities and intervention durations. Methods: Eight databases (CNKI, CSPD, PubMed, Ovid Medline, ProQuest, Cochrane Library, Embase, and Scopus) were searched for randomized controlled trials on altitude training and aerobic capacity following PRISMA guidelines, covering publications up to 15 October 2024. The risk of bias was assessed using Cochrane tools, and a meta-analysis was conducted using Review Manager 5.4 with a random-effects model. Sensitivity and subgroup analyses were performed to identify heterogeneity and influencing factors. Results: Thirteen studies involving 276 participants (aged 18–35) were included. Meta-analysis revealed that compared to low-altitude training, altitude training significantly increased hemoglobin (SMD = 0.7, 95% CI: 0.27–1.13, p = 0.03) and hemoglobin mass (SMD = 0.49, 95% CI: 0.1–0.89, p = 0.16) but had no significant effect on maximal oxygen uptake (SMD = −0.13, 95% CI: −1.21–0.96, p = 0.68). Altitude training also improved performance in trial tests (SMD = −28.73, 95% CI: −58.69–1.23, p = 0.002). Sensitivity analysis confirmed the robustness of hemoglobin and trial test results. Subgroup analysis showed that the “live high, train high” (LHTH) approach and interventions lasting longer than three weeks were most effective in enhancing aerobic capacity. Conclusions: Altitude training improves athletes’ aerobic capacity by enhancing hematological indicators and trial test performance, though its impact on maximal oxygen uptake is minimal. LHTH and interventions exceeding three weeks yield superior outcomes. However, the findings are limited by the number and quality of the available studies. Full article

(This article belongs to the Special Issue Focus on Exercise Physiology and Sports Performance: 2nd Edition)

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18 pages, 6370 KiB

Open AccessReview

Anatomy-Based Filler Injection: Treatment Techniques for Supraorbital Hollowness and Charming Roll

by Gi-Woong Hong, Wonseok Choi, Jovian Wan, Song Eun Yoon, Carlos Bautzer, Lucas Basmage, Patricia Leite and Kyu-Ho Yi

Life 2025, 15(2), 304; https://doi.org/10.3390/life15020304 - 15 Feb 2025

Abstract

Supraorbital hollowness and pretarsal fullness, commonly known as the sunken eyelid and charming roll, respectively, are significant anatomical features that impact the aesthetic appearance of the periorbital region. Supraorbital hollowness is characterized by a recessed appearance of the upper eyelid, often attributed to genetic factors, aging, or surgical alterations, such as excessive fat removal during blepharoplasty. This condition is particularly prevalent among East Asians due to anatomical differences, such as weaker levator muscles and unique fat distribution patterns. Pretarsal fullness, also known as aegyo-sal, enhances the youthful and expressive appearance of the lower eyelid, forming a roll above the lash line that is considered aesthetically desirable in East Asian culture. Anatomical-based filler injection techniques are critical for correcting these features, involving precise placement within the correct tissue planes to avoid complications and achieve natural-looking results. This approach not only improves the aesthetic appeal of the eyelid but also enhances the overall facial harmony, emphasizing the importance of tailored procedures based on individual anatomy and cultural preferences. Full article

(This article belongs to the Special Issue Novel Approaches in Plastic and Reconstructive Surgery to Improve Quality of Life)

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Abstract

Among the Pilates-based exercises, the modified side-arm (MSA) and modified high-five (MHF) are commonly used for shoulder strengthening and rehabilitation. This study examined shoulder girdle muscle activity and ratios across different spring intensities. Twenty-two healthy males performed the MSA and MHF using yellow (low), blue (medium), and red (high) springs. Surface electromyography (EMG) was used to measure serratus anterior (SA), lower trapezius (LT), levator scapulae (LS), upper trapezius (UT), and middle deltoid (MD) muscle activity, along with LS/SA, LS/LT, and UT/LT ratios during concentric, isometric, and eccentric phases. Muscle activities were generally higher in the MHF than in the MSA with the same spring. Both exercises demonstrated a proportional increase in activity with spring intensity, though the activity of the SA and LT in the MHF plateaued. MHF ratios were significantly higher with the red spring. These findings indicate that the MHF stimulates shoulder girdle muscles more than the MSA, and that the MSA can further stimulate shoulder girdle muscles by increasing spring intensity. Additionally, optimal spring intensity exists in the MHF for targeting shoulder stabilization muscles. However, excessive spring intensity during the MHF may lead to abnormal compensation, emphasizing the need for careful spring intensity progression. Full article

25 pages, 4138 KiB

Open AccessArticle

Resveratrol-Enhanced Human Neural Stem Cell-Derived Exosomes Mitigate MPP+-Induced Neurotoxicity Through Activation of AMPK and Nrf2 Pathways and Inhibition of the NLRP3 Inflammasome in SH-SY5Y Cells

by Ming-Chang Chiang, Yu-Ping Yang, Christopher J. B. Nicol, Tairui Chiang and Chiahui Yen

Life 2025, 15(2), 294; https://doi.org/10.3390/life15020294 - 13 Feb 2025

Abstract

Parkinson’s disease (PD) is a progressive neurodegenerative disorder primarily characterized by the loss of dopaminergic neurons in the substantia nigra. Mitochondrial dysfunction, oxidative stress, and neuroinflammation are recognized as critical pathological mechanisms driving neurodegeneration in PD. Exosome (Exo)-based therapies, particularly those derived from human neural stem cells (hNSCs), offer promising neuroprotective effects due to their ability to transfer bioactive molecules that modulate cellular processes. Resveratrol (RES), a polyphenolic compound with potent antioxidant and anti-inflammatory properties, has been shown to enhance the therapeutic potential of stem cell (SC)-derived Exos. This study investigated the neuroprotective effects of RES-treated hNSCs-derived Exos (RES-hNSCs-Exos) on SH-SY5Y cells exposed to 1-methyl-4-phenylpyridinium (MPP⁺), a neurotoxin commonly used to model Parkinsonian neurotoxicity. Treating SH-SY5Y cells with MPP⁺ led to significant reductions in cell viability, mitochondrial dysfunction, increased oxidative stress, and the activation of inflammatory pathways. Treatment with RES-hNSCs-Exos rescued SH-SY5Y cells from MPP⁺-induced toxicity by improving cell viability, enhancing ATP production, increasing mitochondrial biogenesis, and reducing reactive oxygen species (ROS) generation. The findings also demonstrated the increased expression of essential genes involved in mitochondrial biogenesis, such as PGC1α, NRF1, and Tfam, indicating improved mitochondrial function in the presence of RES-hNSCs-Exos. Further analysis revealed that these protective effects were mediated by activating the AMP-activated protein kinase (AMPK) and Nrf2 signaling pathways, which promoted mitochondrial health and reduced oxidative stress. Moreover, RES-hNSCs-Exos treatment suppressed neuroinflammation by downregulating NLRP3 inflammasome activation and reducing the secretion of pro-inflammatory cytokines IL-1β and IL-18. In conclusion, the results suggest that RES-hNSCs-Exos exhibit potent neuroprotective effects against MPP⁺-induced neurotoxicity by enhancing mitochondrial function, reducing oxidative stress, and inhibiting neuroinflammation. These findings highlight the potential of hNSCs-Exos as a novel therapeutic strategy for neurodegenerative diseases like PD, with RES as a valuable enhancer of Exos efficacy. Full article

(This article belongs to the Special Issue Therapeutic Effects of Natural Products on Human Diseases: 2nd Edition)

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