-
Fluorescence Animation
This animation will introduce you to the concept of fluorescence and the reasons why fluorescence-based techniques are used in cell and molecular biology experiments.
published: 14 Oct 2015
-
What is Fluorescence?
Ever wonder what makes your t-shirt glow under a black light? Or why the ink of a highlighter seems un-naturally bright? Dr. Brian Wagner, professor of chemistry at the University of Prince Edward Island, explains the science behind fluorescence, and how we see it around us every day.
Learn more about Dr. Wagner and his research at https://islandscholar.ca/people/bwagner
published: 18 Jul 2013
-
Fluorescence Microscopy Animation
In this animation, you will be introduced to fluorescence microscopy, which is a specialized type of light microscopy.
published: 14 Oct 2015
-
Basics and principle of Fluorescence & Phosphorescence measurement | Learn under 5 min | AI 06
Analytical Instrumentation - Fluorescence & Phosphorescence measurement
----------------------------------------------------------------------------------------------------------
You may have come across terms like fluorescence and phosphorescence. You may have a lot of questions in your head regarding this topic like: What does fluorescent mean?, How does fluorescence works?, How does fluorescence spectroscopy work?, How does fluorescent occur? You will find answers to all these questions in this video.
In the following video, viewers will learn about the basic principle of fluorescence and phosphorescence. The generation of fluorescent and phosphorescent emissions is discussed in detailed using an energy level diagram of a molecule. In the energy level diagram we can see different le...
published: 28 Jul 2017
-
How Does Fluorescence Work?
This week Reactions is exploring the science behind fluorescence. There's a lot of chemistry behind what makes a fluorescent color stand out so brightly from the rest. Today we're digging into what makes them pop, and we're going highlight some of the brilliant applications of fluorescence coming out of nanotechnology.
Check out the NSF's Center for Sustainable Nanotechnology Blog!
http://sustainable-nano.com/
Find us on all these places:
Subscribe! http://bit.ly/ACSReactions
Facebook! http://facebook.com/ACSReactions
Twitter! http://twitter.com/ACSReactions
Tumblr! http://tumblr.com/ACSReactions
Writer/Producer:
Kirk Zamieroski
Executive Producer:
Adam Dylewski
Scientific consultants:
Tom Kuech, Ph.D.
Miriam Krause, Ph.D.
Darcy Gentleman, Ph.D.
Music:
Sam Leopard - Secret Bass
Sa...
published: 12 Jul 2016
-
Know the Glow - phosphorescence vs fluorescence
We experiment with glowing liquids to find out about the different ways they glow! This activity is available in Investigate Box 5: Let it Glow, and the glow liquid is also included in Explore Box 5: Go with the GLow.
published: 29 Aug 2017
-
FISH - Fluorescent In Situ Hybridization
Hey guys,
today I tell you how FISH works.
Cheers, Henrik
Instagram: https://www.instagram.com/king_henrik_the_1st
Literature:
Bartlett, J. M. (2004). Fluorescence in situ hybridization. In Molecular Diagnosis of Cancer (pp. 77-87). Humana Press.
https://www.genome.gov/10000206/fish-fact-sheet/
https://www.neb.com/products/M0303-DNase-I-RNase-free#Product%20Information
published: 21 Dec 2018
-
Explain the principle of Fluorescence and Phosphorescence. | Analytical Chemistry
Many compounds absorb ultraviolet or visible light and undergo an electronic transition from low electronic energy levels to high electronic, energy levels. This Absorption of light requires 10-15 sec.
The instant re-emission of the absorbed energy is called Fluorescence.
While delayed re-emission of the absorbed energy is called Phosphorescence.
For most molecules, the electrons are paired in the ground state i.e. pair of electrons have opposite spins this is called singlet state. As the spin is paired up, they do not show any magnetic moment.
But if the electrons have the same spin the magnetic moment of both the electrons gets combined and they behave like a tiny magnet. This state where the electron pair is having the same spin is called as triplet state.
In a molecule, there are s...
published: 21 Oct 2018
-
Test d'un vieux tube fluorescent OSRAM RED 58W
Salut. Dans cette vidéo, je vous montre un nouveau tube fluorescent en fin de vie.
published: 03 Aug 2024
-
Fluorescence Spectroscopy Tutorial - Basics of Fluorescence
There are different types of spectroscopy methods that you can use, and it can be difficult to choose for a given application. However, when you are dealing with biological material such as analyzing plants, human and animal tissue or perhaps DNA, fluorescence spectroscopy is a good choice for you. Watch this video and learn about the basic principles of Fluorescence spectroscopy.
For more tutorial videos on fluorescence spectroscopy, watch here:
Part One: Basics of Fluorescence: https://www.youtube.com/watch?v=5Q0n27pnQQU&t=2s
Part Two: Common Fluorophores & Instrumentation: https://www.youtube.com/watch?v=C0AydciFoPE
Part Three: Typical Applications: https://www.youtube.com/watch?v=MONv05mJu8Y&t=9s
For more information about fluorescence spectroscopy and our PEBBLE spectrometer read...
published: 17 Feb 2020
2:05
Fluorescence Animation
This animation will introduce you to the concept of fluorescence and the reasons why fluorescence-based techniques are used in cell and molecular biology experi...
This animation will introduce you to the concept of fluorescence and the reasons why fluorescence-based techniques are used in cell and molecular biology experiments.
https://wn.com/Fluorescence_Animation
This animation will introduce you to the concept of fluorescence and the reasons why fluorescence-based techniques are used in cell and molecular biology experiments.
- published: 14 Oct 2015
- views: 73948
2:26
What is Fluorescence?
Ever wonder what makes your t-shirt glow under a black light? Or why the ink of a highlighter seems un-naturally bright? Dr. Brian Wagner, professor of chemistr...
Ever wonder what makes your t-shirt glow under a black light? Or why the ink of a highlighter seems un-naturally bright? Dr. Brian Wagner, professor of chemistry at the University of Prince Edward Island, explains the science behind fluorescence, and how we see it around us every day.
Learn more about Dr. Wagner and his research at https://islandscholar.ca/people/bwagner
https://wn.com/What_Is_Fluorescence
Ever wonder what makes your t-shirt glow under a black light? Or why the ink of a highlighter seems un-naturally bright? Dr. Brian Wagner, professor of chemistry at the University of Prince Edward Island, explains the science behind fluorescence, and how we see it around us every day.
Learn more about Dr. Wagner and his research at https://islandscholar.ca/people/bwagner
- published: 18 Jul 2013
- views: 138210
2:19
Fluorescence Microscopy Animation
In this animation, you will be introduced to fluorescence microscopy, which is a specialized type of light microscopy.
In this animation, you will be introduced to fluorescence microscopy, which is a specialized type of light microscopy.
https://wn.com/Fluorescence_Microscopy_Animation
In this animation, you will be introduced to fluorescence microscopy, which is a specialized type of light microscopy.
- published: 14 Oct 2015
- views: 210579
4:38
Basics and principle of Fluorescence & Phosphorescence measurement | Learn under 5 min | AI 06
Analytical Instrumentation - Fluorescence & Phosphorescence measurement
----------------------------------------------------------------------------------------...
Analytical Instrumentation - Fluorescence & Phosphorescence measurement
----------------------------------------------------------------------------------------------------------
You may have come across terms like fluorescence and phosphorescence. You may have a lot of questions in your head regarding this topic like: What does fluorescent mean?, How does fluorescence works?, How does fluorescence spectroscopy work?, How does fluorescent occur? You will find answers to all these questions in this video.
In the following video, viewers will learn about the basic principle of fluorescence and phosphorescence. The generation of fluorescent and phosphorescent emissions is discussed in detailed using an energy level diagram of a molecule. In the energy level diagram we can see different levels of molecular energies. The electrons when in pair are either in singlet state or triplet state. The basic definitions of singlet state and triplet state is covered and pairing of electrons and their spins is explained in the animation. Once we understand the energy levels corresponding to singlet and triplet state, it becomes quite clear to understand fluorescence and phosphorescence. The fluorescence and phosphorescence emitted by a sample under test is used to perform qualitative and quantitative analysis on the analyte. It is performed using spectrofluorimeter. The basic principle of measurement of fluorescence is also explained in the video.
At the end of the video, viewer will also have a clear idea between the differences in fluorescence and phosphorescence and will be able to compare the two topics.
Hope you like and enjoy the video. Give us a thumbs up. Stay calm and keep learning. Subscribe to the channel to learn about such more interesting concepts and to never miss any new updates.
***
Get amazing content and support on Academic Courses, GATE Courses, Professional Courses and Career with Ekeeda. Go to https://ekeeda.com/ platform and register with the referral code EAPHAR74. Use the coupon code Ninja10 on purchase for special discount!
We have an exciting news for everyone! We have developed a course on PLC basics in collaboration with Internshala where you can get good discounts using the coupon code ISRP16947850. Go check it out here- https://trainings.internshala.com/plc-programming-training
***
-------------------------------------------------------------------------------------------
Please watch the next video: https://youtu.be/B22ZsUOFM98
on Spectrofluorimeter
Please watch the previous video: https://youtu.be/sVc-XIysw0c
on Double Beam Spectrometer
Please watch the video: https://youtu.be/MKFjGIrRKt4
on Basics of UV-Vis spectroscopy in Analytical Instrumentation
Please watch the video: https://youtu.be/pxDbEkspXs0
on Filters and Monochromators, Gratings used in Analytical Instrumentation
Please watch the video: https://youtu.be/YI17AmrR5Iw
on Detectors used in Analytical Instrumentation:
Please watch the video: https://youtu.be/J8Bqyqjlqy0
on Single Beam Photometer used in Analytical Instrumentation
-------------------------------------------------------------------------------------------
Link to the video on Accelerometer:
https://youtu.be/T_iXLNkkjFo
Link to the video on Gyroscope:
https://youtu.be/ti4HEgd4Fgo
Link to the video on Magnetometer:
https://youtu.be/_ZiLwoClRGQ
-------------------------------------------------------------------------------------------
Link to the video #1 in the automation series: https://youtu.be/rFs0zwx_s9g
Introduction to PLC | Programmable logic controllers | Steps towards learning Automation - 01
Link to the video #2 in the automation series: https://youtu.be/fmEmvSazqq8
How does a relay work? | Normally Open | Normally Closed | Steps towards learning Automation - 02
Link to the video #3 in the automation series: https://youtu.be/6sQt1N0nBeE
Concept of Sinking and Sourcing in PLC | Steps towards learning Automation - 03
-------------------------------------------------------------------------------------------
Please watch our playlist on Digital Electronics:
https://www.youtube.com/playlist?list=PLsaRQCHmdZTBd8xnz_XqhHYdFGlnDapC4
Please watch our playlist on Analytical Instrumentation:
https://www.youtube.com/playlist?list=PLsaRQCHmdZTAuxG5X1G9W7zl_p3uwEmY0
Please watch our playlist on Automation and Programmable Logic Controllers (PLC):
https://www.youtube.com/playlist?list=PLsaRQCHmdZTC0HqpVwHnXp9LqzeBDMliy
-------------------------------------------------------------------------------------------
Credits :
https://soundcloud.com/slow-star/robotic-after-all
-~-~~-~~~-~~-~-
Please watch: "What makes our phones smart? - SOC | System on Chip | Brain of a smartphone"
https://www.youtube.com/watch?v=qrT6uSBraEg
-~-~~-~~~-~~-~-
https://wn.com/Basics_And_Principle_Of_Fluorescence_Phosphorescence_Measurement_|_Learn_Under_5_Min_|_Ai_06
Analytical Instrumentation - Fluorescence & Phosphorescence measurement
----------------------------------------------------------------------------------------------------------
You may have come across terms like fluorescence and phosphorescence. You may have a lot of questions in your head regarding this topic like: What does fluorescent mean?, How does fluorescence works?, How does fluorescence spectroscopy work?, How does fluorescent occur? You will find answers to all these questions in this video.
In the following video, viewers will learn about the basic principle of fluorescence and phosphorescence. The generation of fluorescent and phosphorescent emissions is discussed in detailed using an energy level diagram of a molecule. In the energy level diagram we can see different levels of molecular energies. The electrons when in pair are either in singlet state or triplet state. The basic definitions of singlet state and triplet state is covered and pairing of electrons and their spins is explained in the animation. Once we understand the energy levels corresponding to singlet and triplet state, it becomes quite clear to understand fluorescence and phosphorescence. The fluorescence and phosphorescence emitted by a sample under test is used to perform qualitative and quantitative analysis on the analyte. It is performed using spectrofluorimeter. The basic principle of measurement of fluorescence is also explained in the video.
At the end of the video, viewer will also have a clear idea between the differences in fluorescence and phosphorescence and will be able to compare the two topics.
Hope you like and enjoy the video. Give us a thumbs up. Stay calm and keep learning. Subscribe to the channel to learn about such more interesting concepts and to never miss any new updates.
***
Get amazing content and support on Academic Courses, GATE Courses, Professional Courses and Career with Ekeeda. Go to https://ekeeda.com/ platform and register with the referral code EAPHAR74. Use the coupon code Ninja10 on purchase for special discount!
We have an exciting news for everyone! We have developed a course on PLC basics in collaboration with Internshala where you can get good discounts using the coupon code ISRP16947850. Go check it out here- https://trainings.internshala.com/plc-programming-training
***
-------------------------------------------------------------------------------------------
Please watch the next video: https://youtu.be/B22ZsUOFM98
on Spectrofluorimeter
Please watch the previous video: https://youtu.be/sVc-XIysw0c
on Double Beam Spectrometer
Please watch the video: https://youtu.be/MKFjGIrRKt4
on Basics of UV-Vis spectroscopy in Analytical Instrumentation
Please watch the video: https://youtu.be/pxDbEkspXs0
on Filters and Monochromators, Gratings used in Analytical Instrumentation
Please watch the video: https://youtu.be/YI17AmrR5Iw
on Detectors used in Analytical Instrumentation:
Please watch the video: https://youtu.be/J8Bqyqjlqy0
on Single Beam Photometer used in Analytical Instrumentation
-------------------------------------------------------------------------------------------
Link to the video on Accelerometer:
https://youtu.be/T_iXLNkkjFo
Link to the video on Gyroscope:
https://youtu.be/ti4HEgd4Fgo
Link to the video on Magnetometer:
https://youtu.be/_ZiLwoClRGQ
-------------------------------------------------------------------------------------------
Link to the video #1 in the automation series: https://youtu.be/rFs0zwx_s9g
Introduction to PLC | Programmable logic controllers | Steps towards learning Automation - 01
Link to the video #2 in the automation series: https://youtu.be/fmEmvSazqq8
How does a relay work? | Normally Open | Normally Closed | Steps towards learning Automation - 02
Link to the video #3 in the automation series: https://youtu.be/6sQt1N0nBeE
Concept of Sinking and Sourcing in PLC | Steps towards learning Automation - 03
-------------------------------------------------------------------------------------------
Please watch our playlist on Digital Electronics:
https://www.youtube.com/playlist?list=PLsaRQCHmdZTBd8xnz_XqhHYdFGlnDapC4
Please watch our playlist on Analytical Instrumentation:
https://www.youtube.com/playlist?list=PLsaRQCHmdZTAuxG5X1G9W7zl_p3uwEmY0
Please watch our playlist on Automation and Programmable Logic Controllers (PLC):
https://www.youtube.com/playlist?list=PLsaRQCHmdZTC0HqpVwHnXp9LqzeBDMliy
-------------------------------------------------------------------------------------------
Credits :
https://soundcloud.com/slow-star/robotic-after-all
-~-~~-~~~-~~-~-
Please watch: "What makes our phones smart? - SOC | System on Chip | Brain of a smartphone"
https://www.youtube.com/watch?v=qrT6uSBraEg
-~-~~-~~~-~~-~-
- published: 28 Jul 2017
- views: 438064
3:24
How Does Fluorescence Work?
This week Reactions is exploring the science behind fluorescence. There's a lot of chemistry behind what makes a fluorescent color stand out so brightly from th...
This week Reactions is exploring the science behind fluorescence. There's a lot of chemistry behind what makes a fluorescent color stand out so brightly from the rest. Today we're digging into what makes them pop, and we're going highlight some of the brilliant applications of fluorescence coming out of nanotechnology.
Check out the NSF's Center for Sustainable Nanotechnology Blog!
http://sustainable-nano.com/
Find us on all these places:
Subscribe! http://bit.ly/ACSReactions
Facebook! http://facebook.com/ACSReactions
Twitter! http://twitter.com/ACSReactions
Tumblr! http://tumblr.com/ACSReactions
Writer/Producer:
Kirk Zamieroski
Executive Producer:
Adam Dylewski
Scientific consultants:
Tom Kuech, Ph.D.
Miriam Krause, Ph.D.
Darcy Gentleman, Ph.D.
Music:
Sam Leopard - Secret Bass
Sam Leopard - Master of Gold
Sources:
How Do Highlighters work - http://sustainable-nano.com/2013/02/25/how-do-black-lights-work-why-do-highlighters-look-so-bright-and-how-can-you-impress-your-girlfriend-with-science/
What's in Highlighters: Pyranine –
http://www.compoundchem.com/wp-content/uploads/2015/01/The-
Chemistry-of-Highlighter-Pen-Colours.png
The Electromagnetic Spectrum - https://upload.wikimedia.org/wikipedia/commons/thumb/c/cf/EM_Spectrum_Properties_edit.svg/2000px-EM_Spectrum_Properties_edit.svg.png
The Visible Light Spectrum - https://www.colormatters.com/color-and-science/electromagnetic-color
On Tonic water - http://www.livescience.com/36536-tonic-water-quinine-malaria-health.html
On Color –
http://www.livescience.com/32559-why-do-we-see-in-color.html
On Blacklights –
http://www.physics.org/article-questions.asp?id=66
Why flowers take up color from water - http://www.stevespanglerscience.com/lab/experiments/colorful-carnations/
Nanodiamonds - http://sustainable-nano.com/2013/04/24/fluorescent-nanodiamonds-the-movie/#more-870
Fluorescence and Forensics - http://forensics.org.my/img/Article%2004.pdf
Quantum Dots andI Imaging - http://www.ncbi.nlm.nih.gov/pubmed/18393668
http://pubs.acs.org/doi/full/10.1021/nn402581q
Real-Time Background-Free Selective Imaging of Fluorescent Nanodiamonds in Vivo http://pubs.acs.org/doi/full/10.1021/nl302979d
Ever wonder why dogs sniff each others' butts? Or how Adderall works? Or whether it's OK to pee in the pool? We've got you covered: Reactions a web series about the chemistry that surrounds you every day.
Reactions is produced by the American Chemical Society.
https://wn.com/How_Does_Fluorescence_Work
This week Reactions is exploring the science behind fluorescence. There's a lot of chemistry behind what makes a fluorescent color stand out so brightly from the rest. Today we're digging into what makes them pop, and we're going highlight some of the brilliant applications of fluorescence coming out of nanotechnology.
Check out the NSF's Center for Sustainable Nanotechnology Blog!
http://sustainable-nano.com/
Find us on all these places:
Subscribe! http://bit.ly/ACSReactions
Facebook! http://facebook.com/ACSReactions
Twitter! http://twitter.com/ACSReactions
Tumblr! http://tumblr.com/ACSReactions
Writer/Producer:
Kirk Zamieroski
Executive Producer:
Adam Dylewski
Scientific consultants:
Tom Kuech, Ph.D.
Miriam Krause, Ph.D.
Darcy Gentleman, Ph.D.
Music:
Sam Leopard - Secret Bass
Sam Leopard - Master of Gold
Sources:
How Do Highlighters work - http://sustainable-nano.com/2013/02/25/how-do-black-lights-work-why-do-highlighters-look-so-bright-and-how-can-you-impress-your-girlfriend-with-science/
What's in Highlighters: Pyranine –
http://www.compoundchem.com/wp-content/uploads/2015/01/The-
Chemistry-of-Highlighter-Pen-Colours.png
The Electromagnetic Spectrum - https://upload.wikimedia.org/wikipedia/commons/thumb/c/cf/EM_Spectrum_Properties_edit.svg/2000px-EM_Spectrum_Properties_edit.svg.png
The Visible Light Spectrum - https://www.colormatters.com/color-and-science/electromagnetic-color
On Tonic water - http://www.livescience.com/36536-tonic-water-quinine-malaria-health.html
On Color –
http://www.livescience.com/32559-why-do-we-see-in-color.html
On Blacklights –
http://www.physics.org/article-questions.asp?id=66
Why flowers take up color from water - http://www.stevespanglerscience.com/lab/experiments/colorful-carnations/
Nanodiamonds - http://sustainable-nano.com/2013/04/24/fluorescent-nanodiamonds-the-movie/#more-870
Fluorescence and Forensics - http://forensics.org.my/img/Article%2004.pdf
Quantum Dots andI Imaging - http://www.ncbi.nlm.nih.gov/pubmed/18393668
http://pubs.acs.org/doi/full/10.1021/nn402581q
Real-Time Background-Free Selective Imaging of Fluorescent Nanodiamonds in Vivo http://pubs.acs.org/doi/full/10.1021/nl302979d
Ever wonder why dogs sniff each others' butts? Or how Adderall works? Or whether it's OK to pee in the pool? We've got you covered: Reactions a web series about the chemistry that surrounds you every day.
Reactions is produced by the American Chemical Society.
- published: 12 Jul 2016
- views: 141116
0:33
Know the Glow - phosphorescence vs fluorescence
We experiment with glowing liquids to find out about the different ways they glow! This activity is available in Investigate Box 5: Let it Glow, and the glow li...
We experiment with glowing liquids to find out about the different ways they glow! This activity is available in Investigate Box 5: Let it Glow, and the glow liquid is also included in Explore Box 5: Go with the GLow.
https://wn.com/Know_The_Glow_Phosphorescence_Vs_Fluorescence
We experiment with glowing liquids to find out about the different ways they glow! This activity is available in Investigate Box 5: Let it Glow, and the glow liquid is also included in Explore Box 5: Go with the GLow.
- published: 29 Aug 2017
- views: 17687
3:48
FISH - Fluorescent In Situ Hybridization
Hey guys,
today I tell you how FISH works.
Cheers, Henrik
Instagram: https://www.instagram.com/king_henrik_the_1st
Literature:
Bartlett, J. M. (2004). Fluore...
Hey guys,
today I tell you how FISH works.
Cheers, Henrik
Instagram: https://www.instagram.com/king_henrik_the_1st
Literature:
Bartlett, J. M. (2004). Fluorescence in situ hybridization. In Molecular Diagnosis of Cancer (pp. 77-87). Humana Press.
https://www.genome.gov/10000206/fish-fact-sheet/
https://www.neb.com/products/M0303-DNase-I-RNase-free#Product%20Information
https://wn.com/Fish_Fluorescent_In_Situ_Hybridization
Hey guys,
today I tell you how FISH works.
Cheers, Henrik
Instagram: https://www.instagram.com/king_henrik_the_1st
Literature:
Bartlett, J. M. (2004). Fluorescence in situ hybridization. In Molecular Diagnosis of Cancer (pp. 77-87). Humana Press.
https://www.genome.gov/10000206/fish-fact-sheet/
https://www.neb.com/products/M0303-DNase-I-RNase-free#Product%20Information
- published: 21 Dec 2018
- views: 336816
3:54
Explain the principle of Fluorescence and Phosphorescence. | Analytical Chemistry
Many compounds absorb ultraviolet or visible light and undergo an electronic transition from low electronic energy levels to high electronic, energy levels. Thi...
Many compounds absorb ultraviolet or visible light and undergo an electronic transition from low electronic energy levels to high electronic, energy levels. This Absorption of light requires 10-15 sec.
The instant re-emission of the absorbed energy is called Fluorescence.
While delayed re-emission of the absorbed energy is called Phosphorescence.
For most molecules, the electrons are paired in the ground state i.e. pair of electrons have opposite spins this is called singlet state. As the spin is paired up, they do not show any magnetic moment.
But if the electrons have the same spin the magnetic moment of both the electrons gets combined and they behave like a tiny magnet. This state where the electron pair is having the same spin is called as triplet state.
In a molecule, there are several electronic energy levels and electrons are present at the ground state in the singlet state i.e. with opposite spins. When a molecule absorbs U.V. or Visible light, one electron from ground state undergoes to an excited state. Now the excited state is also having several vibrational levels, so the excited electron loses its energy by intermolecular collision and it comes to the lowest vibrational level of the excited state. Finally, the electron from the lowest vibrational level of excited state returns to the ground state by emitting the radiation or light of lower energy or higher wavelength.
This overall process of absorption, intermolecular collision, and emission take 10-4 to 10-8 sec which is very small thus it looks like instant re-emission of light. But the energy of radiation emitted is always less than the energy of radiation absorbed. Or in other words, the wavelength of emitted radiation will be always higher than the wavelength of radiation absorbed. This is Fluorescence phenomenon.
Analytical Reasoning
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English Grammar
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Ionic Equilibria - Physical Chemistry
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Electrochemistry - Physical Chemistry
https://www.youtube.com/watch?v=ifdy9wnF80s&list=PL9AUXQTZw3Sssn-kip7xzKGzwljSRPEX2
Solid State - Physical Chemistry
https://www.youtube.com/watch?v=Pz-yaCYkHZI&list=PL9AUXQTZw3SuoPwu8UFZq4zXiPEC-So6i
Gaseous State - Physical Chemistry
https://www.youtube.com/watch?v=bvp0d8qZCpE&list=PL9AUXQTZw3Ssp6Ne6_XbBu8TLuO7qEd4L
Colloidal States - Physical Chemistry
https://www.youtube.com/watch?v=yjeFUF-fNLQ&list=PL9AUXQTZw3SszL0qFEw1jciS3QIXZB4aZ
Stereochemistry - Organic Chemistry
https://www.youtube.com/watch?v=N5GU6379LCo&list=PL9AUXQTZw3Su_8A-izFKGfBJvSZY7XcCx
Nanomaterials - Engineering Chemistry
https://www.youtube.com/watch?v=eDVwEf2PgRI&list=PL9AUXQTZw3SsYzEUlxhIzmVCvRcyCy9fh
Water and Its Treatment - Engineering Chemistry
https://www.youtube.com/watch?v=eOsZ12nuJnA&list=PL9AUXQTZw3Ss8p3l-b_YIkD-8ZvX4YDsy
Electrochemistry - Engineering Chemistry
https://www.youtube.com/watch?v=oleTF2Qw-gg&list=PL9AUXQTZw3SuETg2vcx6uEYKFwBVJtEot
Environmental Studies
https://www.youtube.com/watch?v=-jm3JOcUwdQ&list=PL9AUXQTZw3SvK27T5RCYJoP_NpndyuEs2
Optics - Applied Physics
https://www.youtube.com/watch?v=XUw7C4AE0Hk&list=PL9AUXQTZw3StU-aX5h47EZTEkOteCjdz6
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https://wn.com/Explain_The_Principle_Of_Fluorescence_And_Phosphorescence._|_Analytical_Chemistry
Many compounds absorb ultraviolet or visible light and undergo an electronic transition from low electronic energy levels to high electronic, energy levels. This Absorption of light requires 10-15 sec.
The instant re-emission of the absorbed energy is called Fluorescence.
While delayed re-emission of the absorbed energy is called Phosphorescence.
For most molecules, the electrons are paired in the ground state i.e. pair of electrons have opposite spins this is called singlet state. As the spin is paired up, they do not show any magnetic moment.
But if the electrons have the same spin the magnetic moment of both the electrons gets combined and they behave like a tiny magnet. This state where the electron pair is having the same spin is called as triplet state.
In a molecule, there are several electronic energy levels and electrons are present at the ground state in the singlet state i.e. with opposite spins. When a molecule absorbs U.V. or Visible light, one electron from ground state undergoes to an excited state. Now the excited state is also having several vibrational levels, so the excited electron loses its energy by intermolecular collision and it comes to the lowest vibrational level of the excited state. Finally, the electron from the lowest vibrational level of excited state returns to the ground state by emitting the radiation or light of lower energy or higher wavelength.
This overall process of absorption, intermolecular collision, and emission take 10-4 to 10-8 sec which is very small thus it looks like instant re-emission of light. But the energy of radiation emitted is always less than the energy of radiation absorbed. Or in other words, the wavelength of emitted radiation will be always higher than the wavelength of radiation absorbed. This is Fluorescence phenomenon.
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- published: 21 Oct 2018
- views: 105114
0:40
Test d'un vieux tube fluorescent OSRAM RED 58W
Salut. Dans cette vidéo, je vous montre un nouveau tube fluorescent en fin de vie.
Salut. Dans cette vidéo, je vous montre un nouveau tube fluorescent en fin de vie.
https://wn.com/Test_D'un_Vieux_Tube_Fluorescent_Osram_Red_58W
Salut. Dans cette vidéo, je vous montre un nouveau tube fluorescent en fin de vie.
- published: 03 Aug 2024
- views: 489
8:02
Fluorescence Spectroscopy Tutorial - Basics of Fluorescence
There are different types of spectroscopy methods that you can use, and it can be difficult to choose for a given application. However, when you are dealing wit...
There are different types of spectroscopy methods that you can use, and it can be difficult to choose for a given application. However, when you are dealing with biological material such as analyzing plants, human and animal tissue or perhaps DNA, fluorescence spectroscopy is a good choice for you. Watch this video and learn about the basic principles of Fluorescence spectroscopy.
For more tutorial videos on fluorescence spectroscopy, watch here:
Part One: Basics of Fluorescence: https://www.youtube.com/watch?v=5Q0n27pnQQU&t=2s
Part Two: Common Fluorophores & Instrumentation: https://www.youtube.com/watch?v=C0AydciFoPE
Part Three: Typical Applications: https://www.youtube.com/watch?v=MONv05mJu8Y&t=9s
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Fluorescence Spectrum: https://ibsen.com/applications/spectroscopy/fluorescence-spectroscopy/fluorescence-spectrum/
Flow Cytometer: https://ibsen.com/applications/spectroscopy/fluorescence-spectroscopy/flow-cytometer/
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https://wn.com/Fluorescence_Spectroscopy_Tutorial_Basics_Of_Fluorescence
There are different types of spectroscopy methods that you can use, and it can be difficult to choose for a given application. However, when you are dealing with biological material such as analyzing plants, human and animal tissue or perhaps DNA, fluorescence spectroscopy is a good choice for you. Watch this video and learn about the basic principles of Fluorescence spectroscopy.
For more tutorial videos on fluorescence spectroscopy, watch here:
Part One: Basics of Fluorescence: https://www.youtube.com/watch?v=5Q0n27pnQQU&t=2s
Part Two: Common Fluorophores & Instrumentation: https://www.youtube.com/watch?v=C0AydciFoPE
Part Three: Typical Applications: https://www.youtube.com/watch?v=MONv05mJu8Y&t=9s
For more information about fluorescence spectroscopy and our PEBBLE spectrometer read here:
PEBBLE Ultra Compact OEM Spectrometers: https://ibsen.com/products/oem-spectrometers/pebble-spectrometers/
Fluorescence Spectroscopy: https://ibsen.com/applications/spectroscopy/fluorescence-spectroscopy/
Fluorescence Instrumentation: https://ibsen.com/applications/spectroscopy/fluorescence-spectroscopy/fluorescence-instrumentation/
Fluorescence Spectrum: https://ibsen.com/applications/spectroscopy/fluorescence-spectroscopy/fluorescence-spectrum/
Flow Cytometer: https://ibsen.com/applications/spectroscopy/fluorescence-spectroscopy/flow-cytometer/
Follow us online:
LinkedIn: https://www.linkedin.com/company/49361/admin/
Twitter: https://twitter.com/IbsenPhotonics
Visit our website: https://ibsen.com/
- published: 17 Feb 2020
- views: 124039