- published: 18 Jul 2013
- views: 145175
-
remove the playlistFluorescence
- remove the playlistFluorescence
Fluorescence
Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation. It is a form of luminescence. In most cases, the emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation. The most striking example of fluorescence occurs when the absorbed radiation is in the ultraviolet region of the spectrum, and thus invisible to the human eye, while the emitted light is in the visible region, which gives the fluorescent substance a distinct color that can only be seen when exposed to UV light. However, unlike phosphorescence, where the substance would continue to glow and emit light for some time after the radiation source has been turned off, fluorescent materials would cease to glow immediately upon removal of the excitation source. Hence, it is not a persistent phenomenon.
Fluorescence has many practical applications, including mineralogy, gemology, chemical sensors (fluorescence spectroscopy), fluorescent labelling, dyes, biological detectors, cosmic-ray detection, and, most commonly, fluorescent lamps. Fluorescence also occurs frequently in nature in some minerals and in various biological states in many branches of the animal kingdom.
This page contains text from Wikipedia, the Free Encyclopedia - https://wn.com/Fluorescence
Fluorescence (album)
Fluorescence is the fourth studio album by New York-based shoegazing band Asobi Seksu. It was released on February 14, 2011. It was released on vinyl through Polyvinyl Record Co., and a limited-edition pink marble vinyl pressing was available for the first 1500 purchases.
Track listing
References
This page contains text from Wikipedia, the Free Encyclopedia - https://wn.com/Fluorescence_(album)
Podcasts:
-
What is Fluorescence?
Ever wonder what makes your t-shirt glow under a black light? Or why the ink of a highlighter seems un-naturally bright? Dr. Brian Wagner, professor of chemistry at the University of Prince Edward Island, explains the science behind fluorescence, and how we see it around us every day. Learn more about Dr. Wagner and his research at https://islandscholar.ca/people/bwagner
published: 18 Jul 2013 -
How Does Fluorescence Work?
This week Reactions is exploring the science behind fluorescence. There's a lot of chemistry behind what makes a fluorescent color stand out so brightly from the rest. Today we're digging into what makes them pop, and we're going highlight some of the brilliant applications of fluorescence coming out of nanotechnology. Check out the NSF's Center for Sustainable Nanotechnology Blog! http://sustainable-nano.com/ Find us on all these places: Subscribe! http://bit.ly/ACSReactions Facebook! http://facebook.com/ACSReactions Twitter! http://twitter.com/ACSReactions Tumblr! http://tumblr.com/ACSReactions Writer/Producer: Kirk Zamieroski Executive Producer: Adam Dylewski Scientific consultants: Tom Kuech, Ph.D. Miriam Krause, Ph.D. Darcy Gentleman, Ph.D. Music: Sam Leopard - Secret Bass Sa...
published: 12 Jul 2016 -
Fluorescence Animation
This animation will introduce you to the concept of fluorescence and the reasons why fluorescence-based techniques are used in cell and molecular biology experiments.
published: 14 Oct 2015 -
How Fluorescence Works - The Science
In this video we explore the colorful science of fluorescence. A really cool way to play with fluorescence at home is get a blue or violet laser pointer and shine it into a dish or jar of water where you have added a drop of fluorescent highlighter fluid. You'll clearly see the beam as the solution fluoresces in its path. Now the common definition of fluorescent is something that glows a visible color when exposed to ultraviolet light. Fluorescence is actually much broader than that and you don't need ultraviolet light in particular. For example in the yellow fluorescent dye "rubrene" both violet and green lasers will activate it and glow yellow. This proves you don't always need ultraviolet light. But a red laser will not activate a yellow dye. Why is that? What's happening in fluore...
published: 24 Dec 2012 -
Explain the principle of Fluorescence and Phosphorescence. | Analytical Chemistry
Many compounds absorb ultraviolet or visible light and undergo an electronic transition from low electronic energy levels to high electronic, energy levels. This Absorption of light requires 10-15 sec. The instant re-emission of the absorbed energy is called Fluorescence. While delayed re-emission of the absorbed energy is called Phosphorescence. For most molecules, the electrons are paired in the ground state i.e. pair of electrons have opposite spins this is called singlet state. As the spin is paired up, they do not show any magnetic moment. But if the electrons have the same spin the magnetic moment of both the electrons gets combined and they behave like a tiny magnet. This state where the electron pair is having the same spin is called as triplet state. In a molecule, there are s...
published: 21 Oct 2018 -
Fluorescence Microscopy Animation
In this animation, you will be introduced to fluorescence microscopy, which is a specialized type of light microscopy.
published: 14 Oct 2015 -
Fluorescence Spectroscopy Tutorial - Basics of Fluorescence
There are different types of spectroscopy methods that you can use, and it can be difficult to choose for a given application. However, when you are dealing with biological material such as analyzing plants, human and animal tissue or perhaps DNA, fluorescence spectroscopy is a good choice for you. Watch this video and learn about the basic principles of Fluorescence spectroscopy. For more tutorial videos on fluorescence spectroscopy, watch here: Part One: Basics of Fluorescence: https://www.youtube.com/watch?v=5Q0n27pnQQU&t=2s Part Two: Common Fluorophores & Instrumentation: https://www.youtube.com/watch?v=C0AydciFoPE Part Three: Typical Applications: https://www.youtube.com/watch?v=MONv05mJu8Y&t=9s For more information about fluorescence spectroscopy and our PEBBLE spectrometer read...
published: 17 Feb 2020 -
Widefield and Confocal Fluorescence Microscopy
We just learned about electron microscopy, so what was the next major innovation in microscopy in the 20th century? That would be fluorescence microscopy, of both the widefield and confocal varieties. How does this work? What is fluorescence in the first place? What are fluorophores? What can we do with this technique? Let's get a closer look now. Script by Kia Mackey Watch the whole Microbiology playlist: http://bit.ly/ProfDaveMicrobio General Chemistry Tutorials: http://bit.ly/ProfDaveGenChem Organic Chemistry Tutorials: http://bit.ly/ProfDaveOrgChem Biochemistry Tutorials: http://bit.ly/ProfDaveBiochem Biology/Genetics Tutorials: http://bit.ly/ProfDaveBio Anatomy & Physiology Tutorials: http://bit.ly/ProfDaveAnatPhys Biopsychology Tutorials: http://bit.ly/ProfDaveBiopsych Immunology ...
published: 15 Feb 2021 -
EOL Philips fluorescent tube
published: 21 Feb 2025 -
Know the Glow - phosphorescence vs fluorescence
We experiment with glowing liquids to find out about the different ways they glow! This activity is available in Investigate Box 5: Let it Glow, and the glow liquid is also included in Explore Box 5: Go with the GLow.
published: 29 Aug 2017
2:26
What is Fluorescence?
- Order: Reorder
- Duration: 2:26
- Uploaded Date: 18 Jul 2013
- views: 145175
Ever wonder what makes your t-shirt glow under a black light? Or why the ink of a highlighter seems un-naturally bright? Dr. Brian Wagner, professor of chemistr...
Ever wonder what makes your t-shirt glow under a black light? Or why the ink of a highlighter seems un-naturally bright? Dr. Brian Wagner, professor of chemistry at the University of Prince Edward Island, explains the science behind fluorescence, and how we see it around us every day.
Learn more about Dr. Wagner and his research at https://islandscholar.ca/people/bwagner
https://wn.com/What_Is_Fluorescence
Ever wonder what makes your t-shirt glow under a black light? Or why the ink of a highlighter seems un-naturally bright? Dr. Brian Wagner, professor of chemistry at the University of Prince Edward Island, explains the science behind fluorescence, and how we see it around us every day.
Learn more about Dr. Wagner and his research at https://islandscholar.ca/people/bwagner
3:24
How Does Fluorescence Work?
- Order: Reorder
- Duration: 3:24
- Uploaded Date: 12 Jul 2016
- views: 146286
This week Reactions is exploring the science behind fluorescence. There's a lot of chemistry behind what makes a fluorescent color stand out so brightly from th...
This week Reactions is exploring the science behind fluorescence. There's a lot of chemistry behind what makes a fluorescent color stand out so brightly from the rest. Today we're digging into what makes them pop, and we're going highlight some of the brilliant applications of fluorescence coming out of nanotechnology.
Check out the NSF's Center for Sustainable Nanotechnology Blog!
http://sustainable-nano.com/
Find us on all these places:
Subscribe! http://bit.ly/ACSReactions
Facebook! http://facebook.com/ACSReactions
Twitter! http://twitter.com/ACSReactions
Tumblr! http://tumblr.com/ACSReactions
Writer/Producer:
Kirk Zamieroski
Executive Producer:
Adam Dylewski
Scientific consultants:
Tom Kuech, Ph.D.
Miriam Krause, Ph.D.
Darcy Gentleman, Ph.D.
Music:
Sam Leopard - Secret Bass
Sam Leopard - Master of Gold
Sources:
How Do Highlighters work - http://sustainable-nano.com/2013/02/25/how-do-black-lights-work-why-do-highlighters-look-so-bright-and-how-can-you-impress-your-girlfriend-with-science/
What's in Highlighters: Pyranine –
http://www.compoundchem.com/wp-content/uploads/2015/01/The-
Chemistry-of-Highlighter-Pen-Colours.png
The Electromagnetic Spectrum - https://upload.wikimedia.org/wikipedia/commons/thumb/c/cf/EM_Spectrum_Properties_edit.svg/2000px-EM_Spectrum_Properties_edit.svg.png
The Visible Light Spectrum - https://www.colormatters.com/color-and-science/electromagnetic-color
On Tonic water - http://www.livescience.com/36536-tonic-water-quinine-malaria-health.html
On Color –
http://www.livescience.com/32559-why-do-we-see-in-color.html
On Blacklights –
http://www.physics.org/article-questions.asp?id=66
Why flowers take up color from water - http://www.stevespanglerscience.com/lab/experiments/colorful-carnations/
Nanodiamonds - http://sustainable-nano.com/2013/04/24/fluorescent-nanodiamonds-the-movie/#more-870
Fluorescence and Forensics - http://forensics.org.my/img/Article%2004.pdf
Quantum Dots andI Imaging - http://www.ncbi.nlm.nih.gov/pubmed/18393668
http://pubs.acs.org/doi/full/10.1021/nn402581q
Real-Time Background-Free Selective Imaging of Fluorescent Nanodiamonds in Vivo http://pubs.acs.org/doi/full/10.1021/nl302979d
Ever wonder why dogs sniff each others' butts? Or how Adderall works? Or whether it's OK to pee in the pool? We've got you covered: Reactions a web series about the chemistry that surrounds you every day.
Reactions is produced by the American Chemical Society.
https://wn.com/How_Does_Fluorescence_Work
This week Reactions is exploring the science behind fluorescence. There's a lot of chemistry behind what makes a fluorescent color stand out so brightly from the rest. Today we're digging into what makes them pop, and we're going highlight some of the brilliant applications of fluorescence coming out of nanotechnology.
Check out the NSF's Center for Sustainable Nanotechnology Blog!
http://sustainable-nano.com/
Find us on all these places:
Subscribe! http://bit.ly/ACSReactions
Facebook! http://facebook.com/ACSReactions
Twitter! http://twitter.com/ACSReactions
Tumblr! http://tumblr.com/ACSReactions
Writer/Producer:
Kirk Zamieroski
Executive Producer:
Adam Dylewski
Scientific consultants:
Tom Kuech, Ph.D.
Miriam Krause, Ph.D.
Darcy Gentleman, Ph.D.
Music:
Sam Leopard - Secret Bass
Sam Leopard - Master of Gold
Sources:
How Do Highlighters work - http://sustainable-nano.com/2013/02/25/how-do-black-lights-work-why-do-highlighters-look-so-bright-and-how-can-you-impress-your-girlfriend-with-science/
What's in Highlighters: Pyranine –
http://www.compoundchem.com/wp-content/uploads/2015/01/The-
Chemistry-of-Highlighter-Pen-Colours.png
The Electromagnetic Spectrum - https://upload.wikimedia.org/wikipedia/commons/thumb/c/cf/EM_Spectrum_Properties_edit.svg/2000px-EM_Spectrum_Properties_edit.svg.png
The Visible Light Spectrum - https://www.colormatters.com/color-and-science/electromagnetic-color
On Tonic water - http://www.livescience.com/36536-tonic-water-quinine-malaria-health.html
On Color –
http://www.livescience.com/32559-why-do-we-see-in-color.html
On Blacklights –
http://www.physics.org/article-questions.asp?id=66
Why flowers take up color from water - http://www.stevespanglerscience.com/lab/experiments/colorful-carnations/
Nanodiamonds - http://sustainable-nano.com/2013/04/24/fluorescent-nanodiamonds-the-movie/#more-870
Fluorescence and Forensics - http://forensics.org.my/img/Article%2004.pdf
Quantum Dots andI Imaging - http://www.ncbi.nlm.nih.gov/pubmed/18393668
http://pubs.acs.org/doi/full/10.1021/nn402581q
Real-Time Background-Free Selective Imaging of Fluorescent Nanodiamonds in Vivo http://pubs.acs.org/doi/full/10.1021/nl302979d
Ever wonder why dogs sniff each others' butts? Or how Adderall works? Or whether it's OK to pee in the pool? We've got you covered: Reactions a web series about the chemistry that surrounds you every day.
Reactions is produced by the American Chemical Society.
- published: 12 Jul 2016
- views: 146286
2:05
Fluorescence Animation
- Order: Reorder
- Duration: 2:05
- Uploaded Date: 14 Oct 2015
- views: 81902
This animation will introduce you to the concept of fluorescence and the reasons why fluorescence-based techniques are used in cell and molecular biology experi...
This animation will introduce you to the concept of fluorescence and the reasons why fluorescence-based techniques are used in cell and molecular biology experiments.
https://wn.com/Fluorescence_Animation
This animation will introduce you to the concept of fluorescence and the reasons why fluorescence-based techniques are used in cell and molecular biology experiments.
- published: 14 Oct 2015
- views: 81902
9:01
How Fluorescence Works - The Science
- Order: Reorder
- Duration: 9:01
- Uploaded Date: 24 Dec 2012
- views: 530747
In this video we explore the colorful science of fluorescence.
A really cool way to play with fluorescence at home is get a blue or violet laser pointer and sh...
In this video we explore the colorful science of fluorescence.
A really cool way to play with fluorescence at home is get a blue or violet laser pointer and shine it into a dish or jar of water where you have added a drop of fluorescent highlighter fluid. You'll clearly see the beam as the solution fluoresces in its path.
Now the common definition of fluorescent is something that glows a visible color when exposed to ultraviolet light. Fluorescence is actually much broader than that and you don't need ultraviolet light in particular. For example in the yellow fluorescent dye "rubrene" both violet and green lasers will activate it and glow yellow. This proves you don't always need ultraviolet light. But a red laser will not activate a yellow dye. Why is that?
What's happening in fluorescence is that the incoming light raises the energy of the electrons in the molecule to an excited state. The electrons then lose a bit of energy due to vibrations of the molecules. And finally the electrons return to the ground state by releasing light. Now since energy cannot be created or destroyed and a bit of energy was already lost as heat in the vibrations of the molecules, the energy of light emitted must have lower energy than the light absorbed.
So since the yellow fluorescent dye emits yellow light, we need to use light of higher energy like violet and green for it to glow. Red is lower energy than yellow light so it can't excite the dye.
It also won't work if you use the same color as the dye like a green laser onto a green dye. This is because you almost always lose a bit of energy and therefore it has to emit a different color or none at all.
Another restriction is that the dye has to also absorb the light to work.
For example Europium Tetrakis (Dibenzoylmethide)Triethylammonium will glow bright orange under violet light. But under green light it doesn't glow at all. This is because the substance simply doesn't absorb green light and so it can't reach an excited state where it can fluoresce. So you need both absorption and higher energy to get fluorescence. Higher energy does not automatically imply absorption.
A really cool trick with absorbance and fluorescence is to get multiple fluorescent dyes of differing colors and shining various wavelengths of light on them. If you start with short, high energy, wavelengths of light you will see all the dyes glow. But as you go to longer wavelengths of lower energy the high-energy dyes will go clear as they can no longer absorb or emit light. When you reach red light all the dyes may look "clear" even though you know they are actually multiple vivid colors in white light.
Now what happens if you mix fluorescent dyes? The results depend on the concentration, absorption and emission profile of the dyes. Sometimes the colors of the resulting fluorescence will simply add up and you get a mix of color. Other times the emission of the higher energy dye will get absorbed by the lower energy dye and the color you get at the end is exclusively the lower energy dye.
Moving on to a new topic now: I said fluorescence worked by first using light to push electrons into high-energy states. Well if we could just push these electrons into their higher energy levels then we don't necessarily need the light. We can do this in a glow stick. A glow stick reaction uses chemicals instead of light to excite the electrons in a fluorescent dye.
Now instead of using chemicals to excite those electrons we can also use direct mechanical grinding. A special range of compounds has this property called triboluminescence.
On a different note: Remember when i said way back that after an electron is excited it loses a bit of energy due to molecular vibrations? It follows that if we can alter or stop these vibrations then we can change the energy of fluorescence and thus its color.
A special dye called pyridine copper iodide exhibits a property called fluorescence thermochromism. It changes fluorescent color with temperature. At room temperature the color is yellow but under liquid nitrogen it changes to blue.
Finally a life saving application of fluorescence is in medical diagnostics. A dye is specially engineered to glow when it comes into contact with a pathogen. Since pathogens usually only occur in tiny invisible concentrations by using one of these special fluorescence dyes a sample to be tested quickly and accurately for the presence of such pathogens. The key point is that fluorescence can still be very bright even at these tiny concentrations.
So there you have it, a cool effect that helps us to understand light, have fun and save lives.
https://wn.com/How_Fluorescence_Works_The_Science
In this video we explore the colorful science of fluorescence.
A really cool way to play with fluorescence at home is get a blue or violet laser pointer and shine it into a dish or jar of water where you have added a drop of fluorescent highlighter fluid. You'll clearly see the beam as the solution fluoresces in its path.
Now the common definition of fluorescent is something that glows a visible color when exposed to ultraviolet light. Fluorescence is actually much broader than that and you don't need ultraviolet light in particular. For example in the yellow fluorescent dye "rubrene" both violet and green lasers will activate it and glow yellow. This proves you don't always need ultraviolet light. But a red laser will not activate a yellow dye. Why is that?
What's happening in fluorescence is that the incoming light raises the energy of the electrons in the molecule to an excited state. The electrons then lose a bit of energy due to vibrations of the molecules. And finally the electrons return to the ground state by releasing light. Now since energy cannot be created or destroyed and a bit of energy was already lost as heat in the vibrations of the molecules, the energy of light emitted must have lower energy than the light absorbed.
So since the yellow fluorescent dye emits yellow light, we need to use light of higher energy like violet and green for it to glow. Red is lower energy than yellow light so it can't excite the dye.
It also won't work if you use the same color as the dye like a green laser onto a green dye. This is because you almost always lose a bit of energy and therefore it has to emit a different color or none at all.
Another restriction is that the dye has to also absorb the light to work.
For example Europium Tetrakis (Dibenzoylmethide)Triethylammonium will glow bright orange under violet light. But under green light it doesn't glow at all. This is because the substance simply doesn't absorb green light and so it can't reach an excited state where it can fluoresce. So you need both absorption and higher energy to get fluorescence. Higher energy does not automatically imply absorption.
A really cool trick with absorbance and fluorescence is to get multiple fluorescent dyes of differing colors and shining various wavelengths of light on them. If you start with short, high energy, wavelengths of light you will see all the dyes glow. But as you go to longer wavelengths of lower energy the high-energy dyes will go clear as they can no longer absorb or emit light. When you reach red light all the dyes may look "clear" even though you know they are actually multiple vivid colors in white light.
Now what happens if you mix fluorescent dyes? The results depend on the concentration, absorption and emission profile of the dyes. Sometimes the colors of the resulting fluorescence will simply add up and you get a mix of color. Other times the emission of the higher energy dye will get absorbed by the lower energy dye and the color you get at the end is exclusively the lower energy dye.
Moving on to a new topic now: I said fluorescence worked by first using light to push electrons into high-energy states. Well if we could just push these electrons into their higher energy levels then we don't necessarily need the light. We can do this in a glow stick. A glow stick reaction uses chemicals instead of light to excite the electrons in a fluorescent dye.
Now instead of using chemicals to excite those electrons we can also use direct mechanical grinding. A special range of compounds has this property called triboluminescence.
On a different note: Remember when i said way back that after an electron is excited it loses a bit of energy due to molecular vibrations? It follows that if we can alter or stop these vibrations then we can change the energy of fluorescence and thus its color.
A special dye called pyridine copper iodide exhibits a property called fluorescence thermochromism. It changes fluorescent color with temperature. At room temperature the color is yellow but under liquid nitrogen it changes to blue.
Finally a life saving application of fluorescence is in medical diagnostics. A dye is specially engineered to glow when it comes into contact with a pathogen. Since pathogens usually only occur in tiny invisible concentrations by using one of these special fluorescence dyes a sample to be tested quickly and accurately for the presence of such pathogens. The key point is that fluorescence can still be very bright even at these tiny concentrations.
So there you have it, a cool effect that helps us to understand light, have fun and save lives.
- published: 24 Dec 2012
- views: 530747
3:54
Explain the principle of Fluorescence and Phosphorescence. | Analytical Chemistry
- Order: Reorder
- Duration: 3:54
- Uploaded Date: 21 Oct 2018
- views: 123342
Many compounds absorb ultraviolet or visible light and undergo an electronic transition from low electronic energy levels to high electronic, energy levels. Thi...
Many compounds absorb ultraviolet or visible light and undergo an electronic transition from low electronic energy levels to high electronic, energy levels. This Absorption of light requires 10-15 sec.
The instant re-emission of the absorbed energy is called Fluorescence.
While delayed re-emission of the absorbed energy is called Phosphorescence.
For most molecules, the electrons are paired in the ground state i.e. pair of electrons have opposite spins this is called singlet state. As the spin is paired up, they do not show any magnetic moment.
But if the electrons have the same spin the magnetic moment of both the electrons gets combined and they behave like a tiny magnet. This state where the electron pair is having the same spin is called as triplet state.
In a molecule, there are several electronic energy levels and electrons are present at the ground state in the singlet state i.e. with opposite spins. When a molecule absorbs U.V. or Visible light, one electron from ground state undergoes to an excited state. Now the excited state is also having several vibrational levels, so the excited electron loses its energy by intermolecular collision and it comes to the lowest vibrational level of the excited state. Finally, the electron from the lowest vibrational level of excited state returns to the ground state by emitting the radiation or light of lower energy or higher wavelength.
This overall process of absorption, intermolecular collision, and emission take 10-4 to 10-8 sec which is very small thus it looks like instant re-emission of light. But the energy of radiation emitted is always less than the energy of radiation absorbed. Or in other words, the wavelength of emitted radiation will be always higher than the wavelength of radiation absorbed. This is Fluorescence phenomenon.
Analytical Reasoning
https://www.youtube.com/watch?v=X-7CQplERYM&list=PL9AUXQTZw3SsoaOllWEuB7ahBesJQTLpE
English Grammar
https://www.youtube.com/watch?v=GmPy-ct2rJ4&list=PL9AUXQTZw3SsCmUjRKccku7xy9u8o3fc9
Interview Skills
https://www.youtube.com/watch?v=Pa_pdAQRZNQ&list=PL9AUXQTZw3SvF80iquuqAhtbX1hTfyqfH
Managerial Economics
https://www.youtube.com/watch?v=U7uDmEe9Bn4&list=PL9AUXQTZw3SsVHepwS2e7W2kjxzlKKjEb
Royalty Free Stock Footage
https://www.youtube.com/watch?v=a1xbYDCDGgI&list=PL9AUXQTZw3SvWSpm0MpIPheeTlg4yUx7m
Chemical Thermodynamics - Physical Chemistry
https://www.youtube.com/watch?v=cATdLP86uHQ&list=PL9AUXQTZw3SsLGGp8p6xeGwM3P9adIRR1
Ionic Equilibria - Physical Chemistry
https://www.youtube.com/watch?v=llTs2-BMmgA&list=PL9AUXQTZw3SuWLAc0-MNpD8Vc-7LyN52w
Electrochemistry - Physical Chemistry
https://www.youtube.com/watch?v=ifdy9wnF80s&list=PL9AUXQTZw3Sssn-kip7xzKGzwljSRPEX2
Solid State - Physical Chemistry
https://www.youtube.com/watch?v=Pz-yaCYkHZI&list=PL9AUXQTZw3SuoPwu8UFZq4zXiPEC-So6i
Gaseous State - Physical Chemistry
https://www.youtube.com/watch?v=bvp0d8qZCpE&list=PL9AUXQTZw3Ssp6Ne6_XbBu8TLuO7qEd4L
Colloidal States - Physical Chemistry
https://www.youtube.com/watch?v=yjeFUF-fNLQ&list=PL9AUXQTZw3SszL0qFEw1jciS3QIXZB4aZ
Stereochemistry - Organic Chemistry
https://www.youtube.com/watch?v=N5GU6379LCo&list=PL9AUXQTZw3Su_8A-izFKGfBJvSZY7XcCx
Nanomaterials - Engineering Chemistry
https://www.youtube.com/watch?v=eDVwEf2PgRI&list=PL9AUXQTZw3SsYzEUlxhIzmVCvRcyCy9fh
Water and Its Treatment - Engineering Chemistry
https://www.youtube.com/watch?v=eOsZ12nuJnA&list=PL9AUXQTZw3Ss8p3l-b_YIkD-8ZvX4YDsy
Electrochemistry - Engineering Chemistry
https://www.youtube.com/watch?v=oleTF2Qw-gg&list=PL9AUXQTZw3SuETg2vcx6uEYKFwBVJtEot
Environmental Studies
https://www.youtube.com/watch?v=-jm3JOcUwdQ&list=PL9AUXQTZw3SvK27T5RCYJoP_NpndyuEs2
Optics - Applied Physics
https://www.youtube.com/watch?v=XUw7C4AE0Hk&list=PL9AUXQTZw3StU-aX5h47EZTEkOteCjdz6
For Details Visit
http://cepekmedia.co.nf
http://cepek.hol.es/
http://edmerls.66Ghz.com/
http://edmerls.tk/
https://wn.com/Explain_The_Principle_Of_Fluorescence_And_Phosphorescence._|_Analytical_Chemistry
Many compounds absorb ultraviolet or visible light and undergo an electronic transition from low electronic energy levels to high electronic, energy levels. This Absorption of light requires 10-15 sec.
The instant re-emission of the absorbed energy is called Fluorescence.
While delayed re-emission of the absorbed energy is called Phosphorescence.
For most molecules, the electrons are paired in the ground state i.e. pair of electrons have opposite spins this is called singlet state. As the spin is paired up, they do not show any magnetic moment.
But if the electrons have the same spin the magnetic moment of both the electrons gets combined and they behave like a tiny magnet. This state where the electron pair is having the same spin is called as triplet state.
In a molecule, there are several electronic energy levels and electrons are present at the ground state in the singlet state i.e. with opposite spins. When a molecule absorbs U.V. or Visible light, one electron from ground state undergoes to an excited state. Now the excited state is also having several vibrational levels, so the excited electron loses its energy by intermolecular collision and it comes to the lowest vibrational level of the excited state. Finally, the electron from the lowest vibrational level of excited state returns to the ground state by emitting the radiation or light of lower energy or higher wavelength.
This overall process of absorption, intermolecular collision, and emission take 10-4 to 10-8 sec which is very small thus it looks like instant re-emission of light. But the energy of radiation emitted is always less than the energy of radiation absorbed. Or in other words, the wavelength of emitted radiation will be always higher than the wavelength of radiation absorbed. This is Fluorescence phenomenon.
Analytical Reasoning
https://www.youtube.com/watch?v=X-7CQplERYM&list=PL9AUXQTZw3SsoaOllWEuB7ahBesJQTLpE
English Grammar
https://www.youtube.com/watch?v=GmPy-ct2rJ4&list=PL9AUXQTZw3SsCmUjRKccku7xy9u8o3fc9
Interview Skills
https://www.youtube.com/watch?v=Pa_pdAQRZNQ&list=PL9AUXQTZw3SvF80iquuqAhtbX1hTfyqfH
Managerial Economics
https://www.youtube.com/watch?v=U7uDmEe9Bn4&list=PL9AUXQTZw3SsVHepwS2e7W2kjxzlKKjEb
Royalty Free Stock Footage
https://www.youtube.com/watch?v=a1xbYDCDGgI&list=PL9AUXQTZw3SvWSpm0MpIPheeTlg4yUx7m
Chemical Thermodynamics - Physical Chemistry
https://www.youtube.com/watch?v=cATdLP86uHQ&list=PL9AUXQTZw3SsLGGp8p6xeGwM3P9adIRR1
Ionic Equilibria - Physical Chemistry
https://www.youtube.com/watch?v=llTs2-BMmgA&list=PL9AUXQTZw3SuWLAc0-MNpD8Vc-7LyN52w
Electrochemistry - Physical Chemistry
https://www.youtube.com/watch?v=ifdy9wnF80s&list=PL9AUXQTZw3Sssn-kip7xzKGzwljSRPEX2
Solid State - Physical Chemistry
https://www.youtube.com/watch?v=Pz-yaCYkHZI&list=PL9AUXQTZw3SuoPwu8UFZq4zXiPEC-So6i
Gaseous State - Physical Chemistry
https://www.youtube.com/watch?v=bvp0d8qZCpE&list=PL9AUXQTZw3Ssp6Ne6_XbBu8TLuO7qEd4L
Colloidal States - Physical Chemistry
https://www.youtube.com/watch?v=yjeFUF-fNLQ&list=PL9AUXQTZw3SszL0qFEw1jciS3QIXZB4aZ
Stereochemistry - Organic Chemistry
https://www.youtube.com/watch?v=N5GU6379LCo&list=PL9AUXQTZw3Su_8A-izFKGfBJvSZY7XcCx
Nanomaterials - Engineering Chemistry
https://www.youtube.com/watch?v=eDVwEf2PgRI&list=PL9AUXQTZw3SsYzEUlxhIzmVCvRcyCy9fh
Water and Its Treatment - Engineering Chemistry
https://www.youtube.com/watch?v=eOsZ12nuJnA&list=PL9AUXQTZw3Ss8p3l-b_YIkD-8ZvX4YDsy
Electrochemistry - Engineering Chemistry
https://www.youtube.com/watch?v=oleTF2Qw-gg&list=PL9AUXQTZw3SuETg2vcx6uEYKFwBVJtEot
Environmental Studies
https://www.youtube.com/watch?v=-jm3JOcUwdQ&list=PL9AUXQTZw3SvK27T5RCYJoP_NpndyuEs2
Optics - Applied Physics
https://www.youtube.com/watch?v=XUw7C4AE0Hk&list=PL9AUXQTZw3StU-aX5h47EZTEkOteCjdz6
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- published: 21 Oct 2018
- views: 123342
2:19
Fluorescence Microscopy Animation
- Order: Reorder
- Duration: 2:19
- Uploaded Date: 14 Oct 2015
- views: 227769
In this animation, you will be introduced to fluorescence microscopy, which is a specialized type of light microscopy.
In this animation, you will be introduced to fluorescence microscopy, which is a specialized type of light microscopy.
https://wn.com/Fluorescence_Microscopy_Animation
In this animation, you will be introduced to fluorescence microscopy, which is a specialized type of light microscopy.
- published: 14 Oct 2015
- views: 227769
8:02
Fluorescence Spectroscopy Tutorial - Basics of Fluorescence
- Order: Reorder
- Duration: 8:02
- Uploaded Date: 17 Feb 2020
- views: 133362
There are different types of spectroscopy methods that you can use, and it can be difficult to choose for a given application. However, when you are dealing wit...
There are different types of spectroscopy methods that you can use, and it can be difficult to choose for a given application. However, when you are dealing with biological material such as analyzing plants, human and animal tissue or perhaps DNA, fluorescence spectroscopy is a good choice for you. Watch this video and learn about the basic principles of Fluorescence spectroscopy.
For more tutorial videos on fluorescence spectroscopy, watch here:
Part One: Basics of Fluorescence: https://www.youtube.com/watch?v=5Q0n27pnQQU&t=2s
Part Two: Common Fluorophores & Instrumentation: https://www.youtube.com/watch?v=C0AydciFoPE
Part Three: Typical Applications: https://www.youtube.com/watch?v=MONv05mJu8Y&t=9s
For more information about fluorescence spectroscopy and our PEBBLE spectrometer read here:
PEBBLE Ultra Compact OEM Spectrometers: https://ibsen.com/products/oem-spectrometers/pebble-spectrometers/
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https://wn.com/Fluorescence_Spectroscopy_Tutorial_Basics_Of_Fluorescence
There are different types of spectroscopy methods that you can use, and it can be difficult to choose for a given application. However, when you are dealing with biological material such as analyzing plants, human and animal tissue or perhaps DNA, fluorescence spectroscopy is a good choice for you. Watch this video and learn about the basic principles of Fluorescence spectroscopy.
For more tutorial videos on fluorescence spectroscopy, watch here:
Part One: Basics of Fluorescence: https://www.youtube.com/watch?v=5Q0n27pnQQU&t=2s
Part Two: Common Fluorophores & Instrumentation: https://www.youtube.com/watch?v=C0AydciFoPE
Part Three: Typical Applications: https://www.youtube.com/watch?v=MONv05mJu8Y&t=9s
For more information about fluorescence spectroscopy and our PEBBLE spectrometer read here:
PEBBLE Ultra Compact OEM Spectrometers: https://ibsen.com/products/oem-spectrometers/pebble-spectrometers/
Fluorescence Spectroscopy: https://ibsen.com/applications/spectroscopy/fluorescence-spectroscopy/
Fluorescence Instrumentation: https://ibsen.com/applications/spectroscopy/fluorescence-spectroscopy/fluorescence-instrumentation/
Fluorescence Spectrum: https://ibsen.com/applications/spectroscopy/fluorescence-spectroscopy/fluorescence-spectrum/
Flow Cytometer: https://ibsen.com/applications/spectroscopy/fluorescence-spectroscopy/flow-cytometer/
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- published: 17 Feb 2020
- views: 133362
9:43
Widefield and Confocal Fluorescence Microscopy
- Order: Reorder
- Duration: 9:43
- Uploaded Date: 15 Feb 2021
- views: 66619
We just learned about electron microscopy, so what was the next major innovation in microscopy in the 20th century? That would be fluorescence microscopy, of bo...
We just learned about electron microscopy, so what was the next major innovation in microscopy in the 20th century? That would be fluorescence microscopy, of both the widefield and confocal varieties. How does this work? What is fluorescence in the first place? What are fluorophores? What can we do with this technique? Let's get a closer look now.
Script by Kia Mackey
Watch the whole Microbiology playlist: http://bit.ly/ProfDaveMicrobio
General Chemistry Tutorials: http://bit.ly/ProfDaveGenChem
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EMAIL► [email protected]
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https://wn.com/Widefield_And_Confocal_Fluorescence_Microscopy
We just learned about electron microscopy, so what was the next major innovation in microscopy in the 20th century? That would be fluorescence microscopy, of both the widefield and confocal varieties. How does this work? What is fluorescence in the first place? What are fluorophores? What can we do with this technique? Let's get a closer look now.
Script by Kia Mackey
Watch the whole Microbiology playlist: http://bit.ly/ProfDaveMicrobio
General Chemistry Tutorials: http://bit.ly/ProfDaveGenChem
Organic Chemistry Tutorials: http://bit.ly/ProfDaveOrgChem
Biochemistry Tutorials: http://bit.ly/ProfDaveBiochem
Biology/Genetics Tutorials: http://bit.ly/ProfDaveBio
Anatomy & Physiology Tutorials: http://bit.ly/ProfDaveAnatPhys
Biopsychology Tutorials: http://bit.ly/ProfDaveBiopsych
Immunology Tutorials: http://bit.ly/ProfDaveImmuno
History of Drugs Videos: http://bit.ly/ProfDaveHistoryDrugs
EMAIL► [email protected]
PATREON► http://patreon.com/ProfessorDaveExplains
Check out "Is This Wi-Fi Organic?", my book on disarming pseudoscience!
Amazon: https://amzn.to/2HtNpVH
Bookshop: https://bit.ly/39cKADM
Barnes and Noble: https://bit.ly/3pUjmrn
Book Depository: http://bit.ly/3aOVDlT
- published: 15 Feb 2021
- views: 66619
0:26
EOL Philips fluorescent tube
- Order: Reorder
- Duration: 0:26
- Uploaded Date: 21 Feb 2025
- views: 137
- published: 21 Feb 2025
- views: 137
0:33
Know the Glow - phosphorescence vs fluorescence
- Order: Reorder
- Duration: 0:33
- Uploaded Date: 29 Aug 2017
- views: 22398
We experiment with glowing liquids to find out about the different ways they glow! This activity is available in Investigate Box 5: Let it Glow, and the glow li...
We experiment with glowing liquids to find out about the different ways they glow! This activity is available in Investigate Box 5: Let it Glow, and the glow liquid is also included in Explore Box 5: Go with the GLow.
https://wn.com/Know_The_Glow_Phosphorescence_Vs_Fluorescence
We experiment with glowing liquids to find out about the different ways they glow! This activity is available in Investigate Box 5: Let it Glow, and the glow liquid is also included in Explore Box 5: Go with the GLow.
- published: 29 Aug 2017
- views: 22398
PLAYLIST TIME:
PLAYLIST TIME:
2:26
What is Fluorescence?
Ever wonder what makes your t-shirt glow under a black light? Or why the ink of a highligh...
published: 18 Jul 2013
What is Fluorescence?
What is Fluorescence?
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- published: 18 Jul 2013
- views: 145175
Ever wonder what makes your t-shirt glow under a black light? Or why the ink of a highlighter seems un-naturally bright? Dr. Brian Wagner, professor of chemistry at the University of Prince Edward Island, explains the science behind fluorescence, and how we see it around us every day.
Learn more about Dr. Wagner and his research at https://islandscholar.ca/people/bwagner
3:24
How Does Fluorescence Work?
This week Reactions is exploring the science behind fluorescence. There's a lot of chemist...
published: 12 Jul 2016
How Does Fluorescence Work?
How Does Fluorescence Work?
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- published: 12 Jul 2016
- views: 146286
This week Reactions is exploring the science behind fluorescence. There's a lot of chemistry behind what makes a fluorescent color stand out so brightly from the rest. Today we're digging into what makes them pop, and we're going highlight some of the brilliant applications of fluorescence coming out of nanotechnology.
Check out the NSF's Center for Sustainable Nanotechnology Blog!
http://sustainable-nano.com/
Find us on all these places:
Subscribe! http://bit.ly/ACSReactions
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Writer/Producer:
Kirk Zamieroski
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Adam Dylewski
Scientific consultants:
Tom Kuech, Ph.D.
Miriam Krause, Ph.D.
Darcy Gentleman, Ph.D.
Music:
Sam Leopard - Secret Bass
Sam Leopard - Master of Gold
Sources:
How Do Highlighters work - http://sustainable-nano.com/2013/02/25/how-do-black-lights-work-why-do-highlighters-look-so-bright-and-how-can-you-impress-your-girlfriend-with-science/
What's in Highlighters: Pyranine –
http://www.compoundchem.com/wp-content/uploads/2015/01/The-
Chemistry-of-Highlighter-Pen-Colours.png
The Electromagnetic Spectrum - https://upload.wikimedia.org/wikipedia/commons/thumb/c/cf/EM_Spectrum_Properties_edit.svg/2000px-EM_Spectrum_Properties_edit.svg.png
The Visible Light Spectrum - https://www.colormatters.com/color-and-science/electromagnetic-color
On Tonic water - http://www.livescience.com/36536-tonic-water-quinine-malaria-health.html
On Color –
http://www.livescience.com/32559-why-do-we-see-in-color.html
On Blacklights –
http://www.physics.org/article-questions.asp?id=66
Why flowers take up color from water - http://www.stevespanglerscience.com/lab/experiments/colorful-carnations/
Nanodiamonds - http://sustainable-nano.com/2013/04/24/fluorescent-nanodiamonds-the-movie/#more-870
Fluorescence and Forensics - http://forensics.org.my/img/Article%2004.pdf
Quantum Dots andI Imaging - http://www.ncbi.nlm.nih.gov/pubmed/18393668
http://pubs.acs.org/doi/full/10.1021/nn402581q
Real-Time Background-Free Selective Imaging of Fluorescent Nanodiamonds in Vivo http://pubs.acs.org/doi/full/10.1021/nl302979d
Ever wonder why dogs sniff each others' butts? Or how Adderall works? Or whether it's OK to pee in the pool? We've got you covered: Reactions a web series about the chemistry that surrounds you every day.
Reactions is produced by the American Chemical Society.
2:05
Fluorescence Animation
This animation will introduce you to the concept of fluorescence and the reasons why fluor...
published: 14 Oct 2015
Fluorescence Animation
Fluorescence Animation
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- published: 14 Oct 2015
- views: 81902
This animation will introduce you to the concept of fluorescence and the reasons why fluorescence-based techniques are used in cell and molecular biology experiments.
9:01
How Fluorescence Works - The Science
In this video we explore the colorful science of fluorescence.
A really cool way to play ...
published: 24 Dec 2012
How Fluorescence Works - The Science
How Fluorescence Works - The Science
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- published: 24 Dec 2012
- views: 530747
In this video we explore the colorful science of fluorescence.
A really cool way to play with fluorescence at home is get a blue or violet laser pointer and shine it into a dish or jar of water where you have added a drop of fluorescent highlighter fluid. You'll clearly see the beam as the solution fluoresces in its path.
Now the common definition of fluorescent is something that glows a visible color when exposed to ultraviolet light. Fluorescence is actually much broader than that and you don't need ultraviolet light in particular. For example in the yellow fluorescent dye "rubrene" both violet and green lasers will activate it and glow yellow. This proves you don't always need ultraviolet light. But a red laser will not activate a yellow dye. Why is that?
What's happening in fluorescence is that the incoming light raises the energy of the electrons in the molecule to an excited state. The electrons then lose a bit of energy due to vibrations of the molecules. And finally the electrons return to the ground state by releasing light. Now since energy cannot be created or destroyed and a bit of energy was already lost as heat in the vibrations of the molecules, the energy of light emitted must have lower energy than the light absorbed.
So since the yellow fluorescent dye emits yellow light, we need to use light of higher energy like violet and green for it to glow. Red is lower energy than yellow light so it can't excite the dye.
It also won't work if you use the same color as the dye like a green laser onto a green dye. This is because you almost always lose a bit of energy and therefore it has to emit a different color or none at all.
Another restriction is that the dye has to also absorb the light to work.
For example Europium Tetrakis (Dibenzoylmethide)Triethylammonium will glow bright orange under violet light. But under green light it doesn't glow at all. This is because the substance simply doesn't absorb green light and so it can't reach an excited state where it can fluoresce. So you need both absorption and higher energy to get fluorescence. Higher energy does not automatically imply absorption.
A really cool trick with absorbance and fluorescence is to get multiple fluorescent dyes of differing colors and shining various wavelengths of light on them. If you start with short, high energy, wavelengths of light you will see all the dyes glow. But as you go to longer wavelengths of lower energy the high-energy dyes will go clear as they can no longer absorb or emit light. When you reach red light all the dyes may look "clear" even though you know they are actually multiple vivid colors in white light.
Now what happens if you mix fluorescent dyes? The results depend on the concentration, absorption and emission profile of the dyes. Sometimes the colors of the resulting fluorescence will simply add up and you get a mix of color. Other times the emission of the higher energy dye will get absorbed by the lower energy dye and the color you get at the end is exclusively the lower energy dye.
Moving on to a new topic now: I said fluorescence worked by first using light to push electrons into high-energy states. Well if we could just push these electrons into their higher energy levels then we don't necessarily need the light. We can do this in a glow stick. A glow stick reaction uses chemicals instead of light to excite the electrons in a fluorescent dye.
Now instead of using chemicals to excite those electrons we can also use direct mechanical grinding. A special range of compounds has this property called triboluminescence.
On a different note: Remember when i said way back that after an electron is excited it loses a bit of energy due to molecular vibrations? It follows that if we can alter or stop these vibrations then we can change the energy of fluorescence and thus its color.
A special dye called pyridine copper iodide exhibits a property called fluorescence thermochromism. It changes fluorescent color with temperature. At room temperature the color is yellow but under liquid nitrogen it changes to blue.
Finally a life saving application of fluorescence is in medical diagnostics. A dye is specially engineered to glow when it comes into contact with a pathogen. Since pathogens usually only occur in tiny invisible concentrations by using one of these special fluorescence dyes a sample to be tested quickly and accurately for the presence of such pathogens. The key point is that fluorescence can still be very bright even at these tiny concentrations.
So there you have it, a cool effect that helps us to understand light, have fun and save lives.
3:54
Explain the principle of Fluorescence and Phosphorescence. | Analytical Chemistry
Many compounds absorb ultraviolet or visible light and undergo an electronic transition fr...
published: 21 Oct 2018
Explain the principle of Fluorescence and Phosphorescence. | Analytical Chemistry
Explain the principle of Fluorescence and Phosphorescence. | Analytical Chemistry
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- published: 21 Oct 2018
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Many compounds absorb ultraviolet or visible light and undergo an electronic transition from low electronic energy levels to high electronic, energy levels. This Absorption of light requires 10-15 sec.
The instant re-emission of the absorbed energy is called Fluorescence.
While delayed re-emission of the absorbed energy is called Phosphorescence.
For most molecules, the electrons are paired in the ground state i.e. pair of electrons have opposite spins this is called singlet state. As the spin is paired up, they do not show any magnetic moment.
But if the electrons have the same spin the magnetic moment of both the electrons gets combined and they behave like a tiny magnet. This state where the electron pair is having the same spin is called as triplet state.
In a molecule, there are several electronic energy levels and electrons are present at the ground state in the singlet state i.e. with opposite spins. When a molecule absorbs U.V. or Visible light, one electron from ground state undergoes to an excited state. Now the excited state is also having several vibrational levels, so the excited electron loses its energy by intermolecular collision and it comes to the lowest vibrational level of the excited state. Finally, the electron from the lowest vibrational level of excited state returns to the ground state by emitting the radiation or light of lower energy or higher wavelength.
This overall process of absorption, intermolecular collision, and emission take 10-4 to 10-8 sec which is very small thus it looks like instant re-emission of light. But the energy of radiation emitted is always less than the energy of radiation absorbed. Or in other words, the wavelength of emitted radiation will be always higher than the wavelength of radiation absorbed. This is Fluorescence phenomenon.
Analytical Reasoning
https://www.youtube.com/watch?v=X-7CQplERYM&list=PL9AUXQTZw3SsoaOllWEuB7ahBesJQTLpE
English Grammar
https://www.youtube.com/watch?v=GmPy-ct2rJ4&list=PL9AUXQTZw3SsCmUjRKccku7xy9u8o3fc9
Interview Skills
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Managerial Economics
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Royalty Free Stock Footage
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Chemical Thermodynamics - Physical Chemistry
https://www.youtube.com/watch?v=cATdLP86uHQ&list=PL9AUXQTZw3SsLGGp8p6xeGwM3P9adIRR1
Ionic Equilibria - Physical Chemistry
https://www.youtube.com/watch?v=llTs2-BMmgA&list=PL9AUXQTZw3SuWLAc0-MNpD8Vc-7LyN52w
Electrochemistry - Physical Chemistry
https://www.youtube.com/watch?v=ifdy9wnF80s&list=PL9AUXQTZw3Sssn-kip7xzKGzwljSRPEX2
Solid State - Physical Chemistry
https://www.youtube.com/watch?v=Pz-yaCYkHZI&list=PL9AUXQTZw3SuoPwu8UFZq4zXiPEC-So6i
Gaseous State - Physical Chemistry
https://www.youtube.com/watch?v=bvp0d8qZCpE&list=PL9AUXQTZw3Ssp6Ne6_XbBu8TLuO7qEd4L
Colloidal States - Physical Chemistry
https://www.youtube.com/watch?v=yjeFUF-fNLQ&list=PL9AUXQTZw3SszL0qFEw1jciS3QIXZB4aZ
Stereochemistry - Organic Chemistry
https://www.youtube.com/watch?v=N5GU6379LCo&list=PL9AUXQTZw3Su_8A-izFKGfBJvSZY7XcCx
Nanomaterials - Engineering Chemistry
https://www.youtube.com/watch?v=eDVwEf2PgRI&list=PL9AUXQTZw3SsYzEUlxhIzmVCvRcyCy9fh
Water and Its Treatment - Engineering Chemistry
https://www.youtube.com/watch?v=eOsZ12nuJnA&list=PL9AUXQTZw3Ss8p3l-b_YIkD-8ZvX4YDsy
Electrochemistry - Engineering Chemistry
https://www.youtube.com/watch?v=oleTF2Qw-gg&list=PL9AUXQTZw3SuETg2vcx6uEYKFwBVJtEot
Environmental Studies
https://www.youtube.com/watch?v=-jm3JOcUwdQ&list=PL9AUXQTZw3SvK27T5RCYJoP_NpndyuEs2
Optics - Applied Physics
https://www.youtube.com/watch?v=XUw7C4AE0Hk&list=PL9AUXQTZw3StU-aX5h47EZTEkOteCjdz6
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2:19
Fluorescence Microscopy Animation
In this animation, you will be introduced to fluorescence microscopy, which is a specializ...
published: 14 Oct 2015
Fluorescence Microscopy Animation
Fluorescence Microscopy Animation
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- published: 14 Oct 2015
- views: 227769
In this animation, you will be introduced to fluorescence microscopy, which is a specialized type of light microscopy.
8:02
Fluorescence Spectroscopy Tutorial - Basics of Fluorescence
There are different types of spectroscopy methods that you can use, and it can be difficul...
published: 17 Feb 2020
Fluorescence Spectroscopy Tutorial - Basics of Fluorescence
Fluorescence Spectroscopy Tutorial - Basics of Fluorescence
- Report rights infringement
- published: 17 Feb 2020
- views: 133362
There are different types of spectroscopy methods that you can use, and it can be difficult to choose for a given application. However, when you are dealing with biological material such as analyzing plants, human and animal tissue or perhaps DNA, fluorescence spectroscopy is a good choice for you. Watch this video and learn about the basic principles of Fluorescence spectroscopy.
For more tutorial videos on fluorescence spectroscopy, watch here:
Part One: Basics of Fluorescence: https://www.youtube.com/watch?v=5Q0n27pnQQU&t=2s
Part Two: Common Fluorophores & Instrumentation: https://www.youtube.com/watch?v=C0AydciFoPE
Part Three: Typical Applications: https://www.youtube.com/watch?v=MONv05mJu8Y&t=9s
For more information about fluorescence spectroscopy and our PEBBLE spectrometer read here:
PEBBLE Ultra Compact OEM Spectrometers: https://ibsen.com/products/oem-spectrometers/pebble-spectrometers/
Fluorescence Spectroscopy: https://ibsen.com/applications/spectroscopy/fluorescence-spectroscopy/
Fluorescence Instrumentation: https://ibsen.com/applications/spectroscopy/fluorescence-spectroscopy/fluorescence-instrumentation/
Fluorescence Spectrum: https://ibsen.com/applications/spectroscopy/fluorescence-spectroscopy/fluorescence-spectrum/
Flow Cytometer: https://ibsen.com/applications/spectroscopy/fluorescence-spectroscopy/flow-cytometer/
Follow us online:
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9:43
Widefield and Confocal Fluorescence Microscopy
We just learned about electron microscopy, so what was the next major innovation in micros...
published: 15 Feb 2021
Widefield and Confocal Fluorescence Microscopy
Widefield and Confocal Fluorescence Microscopy
- Report rights infringement
- published: 15 Feb 2021
- views: 66619
We just learned about electron microscopy, so what was the next major innovation in microscopy in the 20th century? That would be fluorescence microscopy, of both the widefield and confocal varieties. How does this work? What is fluorescence in the first place? What are fluorophores? What can we do with this technique? Let's get a closer look now.
Script by Kia Mackey
Watch the whole Microbiology playlist: http://bit.ly/ProfDaveMicrobio
General Chemistry Tutorials: http://bit.ly/ProfDaveGenChem
Organic Chemistry Tutorials: http://bit.ly/ProfDaveOrgChem
Biochemistry Tutorials: http://bit.ly/ProfDaveBiochem
Biology/Genetics Tutorials: http://bit.ly/ProfDaveBio
Anatomy & Physiology Tutorials: http://bit.ly/ProfDaveAnatPhys
Biopsychology Tutorials: http://bit.ly/ProfDaveBiopsych
Immunology Tutorials: http://bit.ly/ProfDaveImmuno
History of Drugs Videos: http://bit.ly/ProfDaveHistoryDrugs
EMAIL► [email protected]
PATREON► http://patreon.com/ProfessorDaveExplains
Check out "Is This Wi-Fi Organic?", my book on disarming pseudoscience!
Amazon: https://amzn.to/2HtNpVH
Bookshop: https://bit.ly/39cKADM
Barnes and Noble: https://bit.ly/3pUjmrn
Book Depository: http://bit.ly/3aOVDlT
0:26
EOL Philips fluorescent tube
published: 21 Feb 2025
EOL Philips fluorescent tube
0:33
Know the Glow - phosphorescence vs fluorescence
We experiment with glowing liquids to find out about the different ways they glow! This ac...
published: 29 Aug 2017
Know the Glow - phosphorescence vs fluorescence
Know the Glow - phosphorescence vs fluorescence
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- published: 29 Aug 2017
- views: 22398
We experiment with glowing liquids to find out about the different ways they glow! This activity is available in Investigate Box 5: Let it Glow, and the glow liquid is also included in Explore Box 5: Go with the GLow.
Fluorescence
Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation. It is a form of luminescence. In most cases, the emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation. The most striking example of fluorescence occurs when the absorbed radiation is in the ultraviolet region of the spectrum, and thus invisible to the human eye, while the emitted light is in the visible region, which gives the fluorescent substance a distinct color that can only be seen when exposed to UV light. However, unlike phosphorescence, where the substance would continue to glow and emit light for some time after the radiation source has been turned off, fluorescent materials would cease to glow immediately upon removal of the excitation source. Hence, it is not a persistent phenomenon.
Fluorescence has many practical applications, including mineralogy, gemology, chemical sensors (fluorescence spectroscopy), fluorescent labelling, dyes, biological detectors, cosmic-ray detection, and, most commonly, fluorescent lamps. Fluorescence also occurs frequently in nature in some minerals and in various biological states in many branches of the animal kingdom.
This page contains text from Wikipedia, the Free Encyclopedia - https://wn.com/Fluorescence
What is Fluorescence?...
How Does Fluorescence Work?...
Fluorescence Animation...
How Fluorescence Works - The Science...
Explain the principle of Fluorescence and Phospho...
Fluorescence Microscopy Animation...
Fluorescence Spectroscopy Tutorial - Basics of Flu...
Widefield and Confocal Fluorescence Microscopy...
EOL Philips fluorescent tube...
Know the Glow - phosphorescence vs fluorescence...
What Is
by: Richie KotzenI don't love, I don't give, I don't care' cause
I've already been there
I don't want, I don't need, I don't take,
'cause I had it all
I don't guess, I just know, what this is,
is a place to die from
I am not what you see, you are real but not for real
I'm not scared, I'm not high, I'm a man
and I'm all right
We are here, we are gone, and the rest are
left to wonder
What is life, in the hand of illusion, what is life
to a man in confusion
What is right, for a life that is losin', what is time,
what is, what is, what is?
Take look, open up, look inside, what is there
In your heart, in your mind, in your soul
there's the answer
Why you want, why you need, the side effects of
what you see
What is life, in the hand of illusion
What is life, to a man in confusion
What is right for a life that is losin', what is time,
what is, what is, what is?
I don't pay, I just know what this is,