Time-lapse microscopy is time-lapse photography applied to microscopy.
Microscope image sequences are recorded and then viewed at a greater speed to
give an accelerated view of the microscopic process.
Before the introduction of the video tape recorder in the 1960s, time-lapse microscopy recordings
were made on photographic film.
During this period, time-lapse microscopy was referred to as microcinematography.
With the increasing use of video recorders, the term time-lapse video microscopy was gradually adopted.
Today, the term video is increasingly dropped, reflecting that a digital still camera
is used to record the individual image frames, instead of a video recorder.
Applications
Time-lapse microscopy can be used to observe any microscopic object over time.
However, its main use is within cell biology to observe artificially cultured cells. Depending on the cell culture, different microscopy techniques can be applied to enhance characteristics of the cells as most cells are transparent.
Time-lapse photography is a technique whereby the frequency at which film frames are captured (the frame rate) is much lower than that used to view the sequence. When played at normal speed, time appears to be moving faster and thus lapsing. For example, an image of a scene may be captured once every second, then played back at 30 frames per second; the result is an apparent 30 times speed increase. Time-lapse photography can be considered the opposite of high speed photography or slow motion.
Processes that would normally appear subtle to the human eye, e.g. the motion of the sun and stars in the sky, become very pronounced. Time-lapse is the extreme version of the cinematography technique of undercranking, and can be confused with stop motion animation.
History
Some classic subjects of timelapse photography include:
cloudscapes and celestial motion
plants growing and flowers opening
fruit rotting and expiring
evolution of a construction project
people in the city
The technique has been used to photograph crowds, traffic, and even television. The effect of photographing a subject that changes imperceptibly slowly, creates a smooth impression of motion. A subject that changes quickly is transformed into an onslaught of activity.
Time Lapse is the first live album by guitarist Steve Hackett. The album is drawn from live performances at the Savoy Theater in New York City (during the Cured tour) and at Central TV Studios in Nottingham.
Track listing
All songs written by Steve Hackett, except where indicated.
Trouble never seems to go away, So crack a fucking bottle until you're feeling okay. Forget money, politics, and war. Get fucked up, pass out on the floor. Why do we do it? Why not? Sometimes life's not what you thought. My mind is fucking racing it's got to stop. What you handed me works so thanks a lot. Goodbye reality I don't want to be With you anymore We need something to cope. What's cheap, because we're broke. Cheap beer, Saints Ides! Gonna take everything in sight. Joe and Billy up 'till the morning. I'm passed out drunk, lightweight I'm snoring. Time to escape all our problems and threats. Missing our families and dodging our debts. Substance abuse, pass it over here. it's not the time or the place for my head to be clear. Goodbye reality I don't want to be With you anymore We need something to cope. What's cheap, because we're broke.
"In this controlled environment, we employ scanning time-lapse microscopy to observe a large number of individual cells that move through the material," says Rädler ... More information ... DOI. 10.1126/sciadv.adm9195. Journal information. Science Advances ... ....
Using time-lapse microscopy, they studied how these filamentous cells grow in threads that don’t simply divide but multiply up to 14 times, depending on the length of the original mother cell.
matruchotii, using time-lapse microscopy to study how the filamentous cells grow. Rather than just capturing a snapshot of this microbial rainforest, the scientists were able to image bacterial growth dynamics of the miniature ecosystem in real time.
The team used time-lapse microscopy to image the live cells of fruit flies and found a new structure, which forms and disappears in the final stages of a common fruit fly's development, that appears ...
(lLeft) A 25-times magnification of crystallized sugar syrup was taken by Dr ... This category, called Small World in Motion accepts any video or digital time-lapse photography taken through the microscope.
Using live imaging (time-lapse microscopy of living cells) the scientists were able to visualize the fluorescent spots in fly embryos to see how they were moving around to find each other ...DNA organization in real-time.
Time-lapse microscopy allows scientists to detect and track cells, but produces huge amounts of data that is nearly impossible to sort through manually ... Time-lapse microscopy, which captures a series ...
... over long periods of time ... Through their close inspection, using single-cell time-lapse microscopy, Allison and Fang noticed that persisters wake up at an accelerated rate after antibiotic treatment.
The US team used microfluidics and time-lapse microscopy to track the ageing processes across yeast’s lifespan (Picture... The US team used microfluidics and time-lapse microscopy to track the ageing processes across yeast’s lifespan.
Imagine a car that ages either as the engine deteriorates or as the transmission wears out, but not both at the same time ... They developed and employed microfluidics and time-lapse microscopy to track the aging processes across the cell's lifespan.
One experiment produced a still image of blood cells, and another produced a time-lapse series of the growth of a colony of E ... Pengming Song et al, Freeform Illuminator for Computational Microscopy, Intelligent Computing (2023).
But a sophisticated three-dimensional (3D) X-ray microscopy technique combined with a time lapse provided an unprecedented means of studying and quantifying the honeycomb's microstructure.
It is fully motorized with autofocus, allowing for time-lapsed imaging for hours without losing focus. The microscope's precision motorized stage makes it possible for multiple-point time-lapse of various specimens simultaneously.
Live cell imaging is the study of living cells using time-lapse microscopy ...Imaging live cells involves a range of contrast-enhanced imaging methods for optical microscopy.
