Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues or organs under sterile conditions on a nutrient culture medium of known composition. Plant tissue culture is widely used to produce clones of a plant in a method known as micropropagation. Different techniques in plant tissue culture may offer certain advantages over traditional methods of propagation, including:
The production of exact copies of plants that produce particularly good flowers, fruits, or have other desirable traits.
To quickly produce mature plants.
The production of multiples of plants in the absence of seeds or necessary pollinators to produce seeds.
The regeneration of whole plants from plant cells that have been genetically modified.
The production of plants in sterile containers that allows them to be moved with greatly reduced chances of transmitting diseases, pests, and pathogens.
The production of plants from seeds that otherwise have very low chances of germinating and growing, i.e.: orchids and Nepenthes.
Vascular tissue is a complex conducting tissue, formed of more than one cell type, found in vascular plants. The primary components of vascular tissue are the xylem and phloem. These two tissues transport fluid and nutrients internally. There are also two meristems associated with vascular tissue: the vascular cambium and the cork cambium. All the vascular tissues within a particular plant together constitute the vascular tissue system of that plant.
The cells in vascular tissue are typically long and slender. Since the xylem and phloem function in the conduction of water, minerals, and nutrients throughout the plant, it is not surprising that their form should be similar to pipes. The individual cells of phloem are connected end-to-end, just as the sections of a pipe might be. As the plant grows, new vascular tissue differentiates in the growing tips of the plant. The new tissue is aligned with existing vascular tissue, maintaining its connection throughout the plant.
The vascular tissue in plants is arranged in long, discrete strands called vascular bundles. These bundles include both xylem and phloem, as well as supporting and protective cells. In stems and roots, the xylem typically lies closer to the interior of the stem with phloem towards the exterior of the stem. In the stems of some Asteriidae dicots, there may be phloem located inwardly from the xylem as well.
Tissue culture is the growth of tissues or cells separate from the organism. This is typically facilitated via use of a liquid, semi-solid, or solid growth medium, such as broth or agar. Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. The term "tissue culture" was coined by American pathologist Montrose Thomas Burrows, M.D.
Historical usage
In 1885 Wilhelm Roux removed a section of the medullary plate of an embryonicchicken and maintained it in a warm saline solution for several days, establishing the basic principle of tissue culture.
In 1907 the zoologist Ross Granville Harrison demonstrated the growth of frog embryonic cells that would give rise to nerve cells in a medium of clotted lymph.
In 1913, E. Steinhardt, C. Israeli, and R. A. Lambert grew vacciniavirus in fragments of guinea pig corneal tissue. In 1996, the first use of regenerative tissue was used to replace a small distance of a urethra, which led to the understanding that the technique of obtaining samples of tissue, growing it outside the body without a scaffold, and reapplying it, can be used for only small distances of less than 1 cm.
Workbooks for schools from 50p per copy. Visit freesciencelessons.co.uk
This video is based on the AQA spec. If you are following a different exam board then you should check your specification.
In this video, we look at how we can clone plants using cuttings and tissue culture.
Image credits:
Spider plant stolon By Eptalon - Own work, CC BY-SA 3.0, https://commons.wikimedia.org/w/index.php?curid=8952388
Plant cutting "https://commons.wikimedia.org/wiki/File%3AFicus_cuttings_with_roots_in_a_bottle..jpg
By Biusch (Own work) [CC BY-SA 3.0 (https://creativecommons.org/licenses/by-sa/3.0)], via Wikimedia Commons
"
Variegated ficus By Forest & Kim Starr, CC BY 3.0, https://commons.wikimedia.org/w/index.php?curid=6142589
Man carrying out tissue culture "https://commons.wikimedia.org/wiki/...
published: 03 Apr 2018
DIY Tissue Culture: How to Get Started for Less Than $200
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Supplies List Below!
• Scale (0.01g accuracy): https://amzn.to/3I9fDRM
• pH Meter (0.01 pH accuracy): https://amzn.to/41CqJFA
• pH Up & Down: https://amzn.to/3pAM19r
• Spray Bottle: https://amzn.to/3pI3Q6g
• Plastic Gloves: https://amzn.to/41CBBmW
• Forceps: https://amzn.to/42K3VFa
• Scissors: https://amzn.to/3I9dwgK
• Sterile Petri Dishes: https://amzn.to/3nPNHLT
• mL Droppers: https://amzn.to/44X8cXl
• Agar: https://bit.ly/3o4yPcu
• Murashige & Skoog: https://bit.ly/3pMpAOr
• Kinetin: https://bit.ly/41IpXaa
• Polyprop...
published: 14 May 2023
How to Make Plant Tissue Cultures
Asmita Paudel, graduate student Utah State University department of Plants, Soils and Climate.
published: 09 Jul 2020
Introduction to Tissue Culture
Learn about the Perfect Plants proprietary tissue culture practise, why tissue culture is essential to the successful cultivation of any plant on a commercial scale, and how Perfect Plants will help protect the future growth of the cannabis industry.
published: 26 May 2021
Tissue Culture
The technique of tissue culture is a very efficient tool to propagate better improved crops. Cells and tissues of plants are grown invitro in culture medium and are maintained in aseptic conditions. Gottlieb Haberlandt is known as the father of tissue culture. The property of plant cells to grow into a complete plant is known as Totipotency.
To view other chapter videos please purchase from our site https://www.scholarswing.in
published: 06 Dec 2017
How Its Done | Rare Plant TISSUE CULTURE Explained by Nan @kanjarin.greens
In this episode, Nan from @kanjarin.greens from the last episode share with us the process of tissue culture in a way that is casual and digestible even for beginners. This episode was meant to be part of the last episode but I thought to separate it out on its own as it deserve its own spotlight! Never have I met anyone explain tissue culture so eloquently and in a manner that is interesting and fun! Tissue culture is the future of plant production, and is a process responsible for creating new species, mutations, and hybrids. This is an episode not to be missed!
Nursery instagram: @kanjarin.greens
My instagram: @bo.tan.ist
Support me on patreon: http://www.patreon.com/seanfromonlyplants
Jazz background music made by Rhythm Alley
https://www.facebook.com/rhythmalley.taiwan/
Background m...
published: 16 Dec 2022
Plant tissue culture overview |
In this video we would review several methods of plant tissue culture
published: 17 Sep 2020
Plant Tissue Culture 101 | With Demonstration! | The 'Breaking Bad' of Houseplants!
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___ My Stuff ___
These are all plant related products I buy all the time. They are amazon affiliate links which means I do earn a small percentage if you buy any of these products. You do not have to buy any products using these links if you do not wish to support me, that is completely fine! :)
A shortcut to my general Aroid potting mix:
ORCHID BARK: https://amzn.to/2MOsVXS
COIR! : https://amzn.to/33naVtz
PERLITE: https://...
1. Create dynamism in Sugarcane crop activities.
2. Sugarcane grows well even in adverse conditions.
3. Sugarcane produces long and plentiful shoots quickly.
4. Sugarcane shoots thrive and the germination rate increases.
5. Increases absorption of primary, secondary, and micronutrients and increases their intracellular transport.
6. The process of cell division is accelerated, so the leaves become thicker, wider, and longer. As a result, the rate of photosynthesis increases.
7. Increased immunity makes sugarcane less susceptible to diseases and insects.
8. Increases the number of canes per acre, stalk length, and thickness and helps increase yield.
✅ Website👇
www.gannamaster.com
☎️ Customer Care - 74700 51005
#गन्नामास्टर #ऊसशेती #मास्टररिकव्हर #गन्नामास्टरकीट
published: 28 Jul 2023
DIY Tissue Culture PART 2: The BioCoupler
Live. Laugh. Autoclave. Plants are science! Let's propagate them the scientific way.
Find me on Instagram: https://www.instagram.com/plantsinjars_tissueculture/
Where I buy tissue culture supplies: https://www.plantcelltechnology.com/?ref=PLANTSINJARS
Supplies List:
• The BioCoupler: https://bit.ly/3p2bvwj
• Murashige & Skoog: https://bit.ly/3pMpAOr
• Kinetin: https://bit.ly/41IpXaa
• BAP: https://bit.ly/3MYJ2zv
• IBA: https://bit.ly/3N0cghE
• Scale (0.01g accuracy): https://amzn.to/3I9fDRM
• pH Meter: https://amzn.to/3NkTiU6
• pH Up & Down: https://amzn.to/3pAM19r
• Forceps: https://amzn.to/42K3VFa
• mL Droppers: https://amzn.to/44X8cXl
Use code PLANTSINJARS for 10% off your Plant Cell Technology order.
Protocol: https://journals.ashs.org/hortsci/view/journals/hortsci/5...
Workbooks for schools from 50p per copy. Visit freesciencelessons.co.uk
This video is based on the AQA spec. If you are following a different exam board then y...
Workbooks for schools from 50p per copy. Visit freesciencelessons.co.uk
This video is based on the AQA spec. If you are following a different exam board then you should check your specification.
In this video, we look at how we can clone plants using cuttings and tissue culture.
Image credits:
Spider plant stolon By Eptalon - Own work, CC BY-SA 3.0, https://commons.wikimedia.org/w/index.php?curid=8952388
Plant cutting "https://commons.wikimedia.org/wiki/File%3AFicus_cuttings_with_roots_in_a_bottle..jpg
By Biusch (Own work) [CC BY-SA 3.0 (https://creativecommons.org/licenses/by-sa/3.0)], via Wikimedia Commons
"
Variegated ficus By Forest & Kim Starr, CC BY 3.0, https://commons.wikimedia.org/w/index.php?curid=6142589
Man carrying out tissue culture "https://commons.wikimedia.org/wiki/File%3AResearch.jpg
By Tatpong Tulyanon (Digital camera) [CC0], via Wikimedia Commons"
Tiny plants from tissue culture "https://commons.wikimedia.org/wiki/File%3APhyscomitrella_growing_on_agar_plates.jpg
By Sabisteb (Anja Martin from the Ralf Reski lab) [CC BY-SA 1.0 (https://creativecommons.org/licenses/by-sa/1.0)], via Wikimedia Commons"
Petunias By Gardenandgreen.co.uk - Own work, CC BY-SA 3.0, https://commons.wikimedia.org/w/index.php?curid=31823028
Music credit:
Deliberate Thought by Kevin MacLeod is licensed under a Creative Commons Attribution licence (https://creativecommons.org/licenses/by/4.0/)
Source: http://incompetech.com/music/royalty-free/?keywords=deliberate+thought
Artist: http://incompetech.com/
Workbooks for schools from 50p per copy. Visit freesciencelessons.co.uk
This video is based on the AQA spec. If you are following a different exam board then you should check your specification.
In this video, we look at how we can clone plants using cuttings and tissue culture.
Image credits:
Spider plant stolon By Eptalon - Own work, CC BY-SA 3.0, https://commons.wikimedia.org/w/index.php?curid=8952388
Plant cutting "https://commons.wikimedia.org/wiki/File%3AFicus_cuttings_with_roots_in_a_bottle..jpg
By Biusch (Own work) [CC BY-SA 3.0 (https://creativecommons.org/licenses/by-sa/3.0)], via Wikimedia Commons
"
Variegated ficus By Forest & Kim Starr, CC BY 3.0, https://commons.wikimedia.org/w/index.php?curid=6142589
Man carrying out tissue culture "https://commons.wikimedia.org/wiki/File%3AResearch.jpg
By Tatpong Tulyanon (Digital camera) [CC0], via Wikimedia Commons"
Tiny plants from tissue culture "https://commons.wikimedia.org/wiki/File%3APhyscomitrella_growing_on_agar_plates.jpg
By Sabisteb (Anja Martin from the Ralf Reski lab) [CC BY-SA 1.0 (https://creativecommons.org/licenses/by-sa/1.0)], via Wikimedia Commons"
Petunias By Gardenandgreen.co.uk - Own work, CC BY-SA 3.0, https://commons.wikimedia.org/w/index.php?curid=31823028
Music credit:
Deliberate Thought by Kevin MacLeod is licensed under a Creative Commons Attribution licence (https://creativecommons.org/licenses/by/4.0/)
Source: http://incompetech.com/music/royalty-free/?keywords=deliberate+thought
Artist: http://incompetech.com/
I sacrificed a finger to bring you this video. please subscribe
Shop my Laboratory: https://bit.ly/3NR6IqA
NEW MERCH: https://bit.ly/PIJ-shirt
Join the Plant...
I sacrificed a finger to bring you this video. please subscribe
Shop my Laboratory: https://bit.ly/3NR6IqA
NEW MERCH: https://bit.ly/PIJ-shirt
Join the Plants in Jars discord server! https://discord.gg/7mJgZemUJM
https://www.instagram.com/plantsinjars_tc/
Supplies List Below!
• Scale (0.01g accuracy): https://amzn.to/3I9fDRM
• pH Meter (0.01 pH accuracy): https://amzn.to/41CqJFA
• pH Up & Down: https://amzn.to/3pAM19r
• Spray Bottle: https://amzn.to/3pI3Q6g
• Plastic Gloves: https://amzn.to/41CBBmW
• Forceps: https://amzn.to/42K3VFa
• Scissors: https://amzn.to/3I9dwgK
• Sterile Petri Dishes: https://amzn.to/3nPNHLT
• mL Droppers: https://amzn.to/44X8cXl
• Agar: https://bit.ly/3o4yPcu
• Murashige & Skoog: https://bit.ly/3pMpAOr
• Kinetin: https://bit.ly/41IpXaa
• Polypropylene Containers: https://bit.ly/3pJrlMF
• Light Meter: https://amzn.to/42B1THI
Protocol for video: https://www.researchgate.net/publication/257148024_Micropropagation_of_self-heading_Philodendron_via_direct_shoot_regeneration
(I am aware that Philodendron Mamei is not technically a "self heading philodendron", but this protocol has worked well for me before for other similar philos!)
Timestamps:
• 1:31 Building a still air box
• 3:18 Tissue culture supplies
• 4:45 What is a tissue culture protocol?
• 5:03 Where to find tissue culture protocols
• 5:44 What is tissue culture media?
• 8:55 How to read a tissue culture protocol
• 11:00 Making tissue culture media
• 13:52 Sterilizing the media
• 17:19 Prepping the still air box
• 18:30 Preparing the explants (tissue samples)
• 19:39 Putting the explant into tissue culture
• 25:30 Conditions for growth
• 26:19 When to subculture
Please note that some of the links in the description are affiliate links, which means that if you click on one of the product links and make a purchase, I may receive a commission. This commission comes at no additional cost to you.
I sacrificed a finger to bring you this video. please subscribe
Shop my Laboratory: https://bit.ly/3NR6IqA
NEW MERCH: https://bit.ly/PIJ-shirt
Join the Plants in Jars discord server! https://discord.gg/7mJgZemUJM
https://www.instagram.com/plantsinjars_tc/
Supplies List Below!
• Scale (0.01g accuracy): https://amzn.to/3I9fDRM
• pH Meter (0.01 pH accuracy): https://amzn.to/41CqJFA
• pH Up & Down: https://amzn.to/3pAM19r
• Spray Bottle: https://amzn.to/3pI3Q6g
• Plastic Gloves: https://amzn.to/41CBBmW
• Forceps: https://amzn.to/42K3VFa
• Scissors: https://amzn.to/3I9dwgK
• Sterile Petri Dishes: https://amzn.to/3nPNHLT
• mL Droppers: https://amzn.to/44X8cXl
• Agar: https://bit.ly/3o4yPcu
• Murashige & Skoog: https://bit.ly/3pMpAOr
• Kinetin: https://bit.ly/41IpXaa
• Polypropylene Containers: https://bit.ly/3pJrlMF
• Light Meter: https://amzn.to/42B1THI
Protocol for video: https://www.researchgate.net/publication/257148024_Micropropagation_of_self-heading_Philodendron_via_direct_shoot_regeneration
(I am aware that Philodendron Mamei is not technically a "self heading philodendron", but this protocol has worked well for me before for other similar philos!)
Timestamps:
• 1:31 Building a still air box
• 3:18 Tissue culture supplies
• 4:45 What is a tissue culture protocol?
• 5:03 Where to find tissue culture protocols
• 5:44 What is tissue culture media?
• 8:55 How to read a tissue culture protocol
• 11:00 Making tissue culture media
• 13:52 Sterilizing the media
• 17:19 Prepping the still air box
• 18:30 Preparing the explants (tissue samples)
• 19:39 Putting the explant into tissue culture
• 25:30 Conditions for growth
• 26:19 When to subculture
Please note that some of the links in the description are affiliate links, which means that if you click on one of the product links and make a purchase, I may receive a commission. This commission comes at no additional cost to you.
Learn about the Perfect Plants proprietary tissue culture practise, why tissue culture is essential to the successful cultivation of any plant on a commercial s...
Learn about the Perfect Plants proprietary tissue culture practise, why tissue culture is essential to the successful cultivation of any plant on a commercial scale, and how Perfect Plants will help protect the future growth of the cannabis industry.
Learn about the Perfect Plants proprietary tissue culture practise, why tissue culture is essential to the successful cultivation of any plant on a commercial scale, and how Perfect Plants will help protect the future growth of the cannabis industry.
The technique of tissue culture is a very efficient tool to propagate better improved crops. Cells and tissues of plants are grown invitro in culture medium and...
The technique of tissue culture is a very efficient tool to propagate better improved crops. Cells and tissues of plants are grown invitro in culture medium and are maintained in aseptic conditions. Gottlieb Haberlandt is known as the father of tissue culture. The property of plant cells to grow into a complete plant is known as Totipotency.
To view other chapter videos please purchase from our site https://www.scholarswing.in
The technique of tissue culture is a very efficient tool to propagate better improved crops. Cells and tissues of plants are grown invitro in culture medium and are maintained in aseptic conditions. Gottlieb Haberlandt is known as the father of tissue culture. The property of plant cells to grow into a complete plant is known as Totipotency.
To view other chapter videos please purchase from our site https://www.scholarswing.in
In this episode, Nan from @kanjarin.greens from the last episode share with us the process of tissue culture in a way that is casual and digestible even for beg...
In this episode, Nan from @kanjarin.greens from the last episode share with us the process of tissue culture in a way that is casual and digestible even for beginners. This episode was meant to be part of the last episode but I thought to separate it out on its own as it deserve its own spotlight! Never have I met anyone explain tissue culture so eloquently and in a manner that is interesting and fun! Tissue culture is the future of plant production, and is a process responsible for creating new species, mutations, and hybrids. This is an episode not to be missed!
Nursery instagram: @kanjarin.greens
My instagram: @bo.tan.ist
Support me on patreon: http://www.patreon.com/seanfromonlyplants
Jazz background music made by Rhythm Alley
https://www.facebook.com/rhythmalley.taiwan/
Background music are created by me exclusively for this channel
In this episode, Nan from @kanjarin.greens from the last episode share with us the process of tissue culture in a way that is casual and digestible even for beginners. This episode was meant to be part of the last episode but I thought to separate it out on its own as it deserve its own spotlight! Never have I met anyone explain tissue culture so eloquently and in a manner that is interesting and fun! Tissue culture is the future of plant production, and is a process responsible for creating new species, mutations, and hybrids. This is an episode not to be missed!
Nursery instagram: @kanjarin.greens
My instagram: @bo.tan.ist
Support me on patreon: http://www.patreon.com/seanfromonlyplants
Jazz background music made by Rhythm Alley
https://www.facebook.com/rhythmalley.taiwan/
Background music are created by me exclusively for this channel
I HAVE MERCH!
https://teespring.com/stores/kayleeellen
Follow me on instagram: https://www.instagram.com/kayleeellenofficial
My Rare Plant Shop!
website: ...
I HAVE MERCH!
https://teespring.com/stores/kayleeellen
Follow me on instagram: https://www.instagram.com/kayleeellenofficial
My Rare Plant Shop!
website: https://www.therareplantshop.co.uk/
instagram: https://www.instagram.com/therareplantshop/
Etsy: https://www.etsy.com/uk/shop/TheRarePlantShop
Facebook: https://www.facebook.com/therareplantshop
___ My Stuff ___
These are all plant related products I buy all the time. They are amazon affiliate links which means I do earn a small percentage if you buy any of these products. You do not have to buy any products using these links if you do not wish to support me, that is completely fine! :)
A shortcut to my general Aroid potting mix:
ORCHID BARK: https://amzn.to/2MOsVXS
COIR! : https://amzn.to/33naVtz
PERLITE: https://amzn.to/2YRKlJH
ACTIVATED CHARCOAL: https://amzn.to/2KxlgdO
WORM CASTINGS: https://amzn.to/2YM3lJF
SPHAGNUM MOSS: https://amzn.to/2MRnzLq
Coir (Coconut Husk) SMALL - if you don't need much: https://amzn.to/2PkQoyZ
Coir (Coconut Husk) LARGE: https://amzn.to/2Iw3d9l
Perlite: https://amzn.to/2IryzxX
My Plant Pots: https://amzn.to/2VTAgr1
My moisture probe: https://amzn.to/2NO909X
My Large Humidifier: https://amzn.to/2TpWLq3
My Smaller (Alexa) Humidifier: https://amzn.to/2NQmUZ9
Thermometer/Hygrometer: https://amzn.to/2Hrh4My
My Clip on Grow Lights: https://amzn.to/2DxWWFP
Some really good cheap Elho outer pots I use: https://amzn.to/2GBK3eP
I HAVE MERCH!
https://teespring.com/stores/kayleeellen
Follow me on instagram: https://www.instagram.com/kayleeellenofficial
My Rare Plant Shop!
website: https://www.therareplantshop.co.uk/
instagram: https://www.instagram.com/therareplantshop/
Etsy: https://www.etsy.com/uk/shop/TheRarePlantShop
Facebook: https://www.facebook.com/therareplantshop
___ My Stuff ___
These are all plant related products I buy all the time. They are amazon affiliate links which means I do earn a small percentage if you buy any of these products. You do not have to buy any products using these links if you do not wish to support me, that is completely fine! :)
A shortcut to my general Aroid potting mix:
ORCHID BARK: https://amzn.to/2MOsVXS
COIR! : https://amzn.to/33naVtz
PERLITE: https://amzn.to/2YRKlJH
ACTIVATED CHARCOAL: https://amzn.to/2KxlgdO
WORM CASTINGS: https://amzn.to/2YM3lJF
SPHAGNUM MOSS: https://amzn.to/2MRnzLq
Coir (Coconut Husk) SMALL - if you don't need much: https://amzn.to/2PkQoyZ
Coir (Coconut Husk) LARGE: https://amzn.to/2Iw3d9l
Perlite: https://amzn.to/2IryzxX
My Plant Pots: https://amzn.to/2VTAgr1
My moisture probe: https://amzn.to/2NO909X
My Large Humidifier: https://amzn.to/2TpWLq3
My Smaller (Alexa) Humidifier: https://amzn.to/2NQmUZ9
Thermometer/Hygrometer: https://amzn.to/2Hrh4My
My Clip on Grow Lights: https://amzn.to/2DxWWFP
Some really good cheap Elho outer pots I use: https://amzn.to/2GBK3eP
1. Create dynamism in Sugarcane crop activities.
2. Sugarcane grows well even in adverse conditions.
3. Sugarcane produces long and plentiful shoots quickly.
4....
1. Create dynamism in Sugarcane crop activities.
2. Sugarcane grows well even in adverse conditions.
3. Sugarcane produces long and plentiful shoots quickly.
4. Sugarcane shoots thrive and the germination rate increases.
5. Increases absorption of primary, secondary, and micronutrients and increases their intracellular transport.
6. The process of cell division is accelerated, so the leaves become thicker, wider, and longer. As a result, the rate of photosynthesis increases.
7. Increased immunity makes sugarcane less susceptible to diseases and insects.
8. Increases the number of canes per acre, stalk length, and thickness and helps increase yield.
✅ Website👇
www.gannamaster.com
☎️ Customer Care - 74700 51005
#गन्नामास्टर #ऊसशेती #मास्टररिकव्हर #गन्नामास्टरकीट
1. Create dynamism in Sugarcane crop activities.
2. Sugarcane grows well even in adverse conditions.
3. Sugarcane produces long and plentiful shoots quickly.
4. Sugarcane shoots thrive and the germination rate increases.
5. Increases absorption of primary, secondary, and micronutrients and increases their intracellular transport.
6. The process of cell division is accelerated, so the leaves become thicker, wider, and longer. As a result, the rate of photosynthesis increases.
7. Increased immunity makes sugarcane less susceptible to diseases and insects.
8. Increases the number of canes per acre, stalk length, and thickness and helps increase yield.
✅ Website👇
www.gannamaster.com
☎️ Customer Care - 74700 51005
#गन्नामास्टर #ऊसशेती #मास्टररिकव्हर #गन्नामास्टरकीट
Live. Laugh. Autoclave. Plants are science! Let's propagate them the scientific way.
Find me on Instagram: https://www.instagram.com/plantsinjars_tissuecultur...
Live. Laugh. Autoclave. Plants are science! Let's propagate them the scientific way.
Find me on Instagram: https://www.instagram.com/plantsinjars_tissueculture/
Where I buy tissue culture supplies: https://www.plantcelltechnology.com/?ref=PLANTSINJARS
Supplies List:
• The BioCoupler: https://bit.ly/3p2bvwj
• Murashige & Skoog: https://bit.ly/3pMpAOr
• Kinetin: https://bit.ly/41IpXaa
• BAP: https://bit.ly/3MYJ2zv
• IBA: https://bit.ly/3N0cghE
• Scale (0.01g accuracy): https://amzn.to/3I9fDRM
• pH Meter: https://amzn.to/3NkTiU6
• pH Up & Down: https://amzn.to/3pAM19r
• Forceps: https://amzn.to/42K3VFa
• mL Droppers: https://amzn.to/44X8cXl
Use code PLANTSINJARS for 10% off your Plant Cell Technology order.
Protocol: https://journals.ashs.org/hortsci/view/journals/hortsci/55/3/article-p294.xml
This protocol uses PPFD to measure light. To convert PPFD to lux, I use this calculator: https://www.waveformlighting.com/horticulture/convert-ppfd-to-lux-online-calculator
Timestamps:
1:51: What is the BioCoupler?
3:09: Why does the BioCoupler work?
4:13: Overview of the Tissue Culture Process
6:19: How to Make Liquid Tissue Culture Media
10:16: How to Sterilize a BioCoupler
12:44: Setting up the Still Air Transfer Box
14:06: Still Air Box Demo
16:49: Laminar Flow Hood Demo
17:40: Bonus Experiment
Please note that some of the links in the description are affiliate links, which means that if you click on one of the product links and make a purchase, I may receive a commission. This commission comes at no additional cost to you.
Live. Laugh. Autoclave. Plants are science! Let's propagate them the scientific way.
Find me on Instagram: https://www.instagram.com/plantsinjars_tissueculture/
Where I buy tissue culture supplies: https://www.plantcelltechnology.com/?ref=PLANTSINJARS
Supplies List:
• The BioCoupler: https://bit.ly/3p2bvwj
• Murashige & Skoog: https://bit.ly/3pMpAOr
• Kinetin: https://bit.ly/41IpXaa
• BAP: https://bit.ly/3MYJ2zv
• IBA: https://bit.ly/3N0cghE
• Scale (0.01g accuracy): https://amzn.to/3I9fDRM
• pH Meter: https://amzn.to/3NkTiU6
• pH Up & Down: https://amzn.to/3pAM19r
• Forceps: https://amzn.to/42K3VFa
• mL Droppers: https://amzn.to/44X8cXl
Use code PLANTSINJARS for 10% off your Plant Cell Technology order.
Protocol: https://journals.ashs.org/hortsci/view/journals/hortsci/55/3/article-p294.xml
This protocol uses PPFD to measure light. To convert PPFD to lux, I use this calculator: https://www.waveformlighting.com/horticulture/convert-ppfd-to-lux-online-calculator
Timestamps:
1:51: What is the BioCoupler?
3:09: Why does the BioCoupler work?
4:13: Overview of the Tissue Culture Process
6:19: How to Make Liquid Tissue Culture Media
10:16: How to Sterilize a BioCoupler
12:44: Setting up the Still Air Transfer Box
14:06: Still Air Box Demo
16:49: Laminar Flow Hood Demo
17:40: Bonus Experiment
Please note that some of the links in the description are affiliate links, which means that if you click on one of the product links and make a purchase, I may receive a commission. This commission comes at no additional cost to you.
Workbooks for schools from 50p per copy. Visit freesciencelessons.co.uk
This video is based on the AQA spec. If you are following a different exam board then you should check your specification.
In this video, we look at how we can clone plants using cuttings and tissue culture.
Image credits:
Spider plant stolon By Eptalon - Own work, CC BY-SA 3.0, https://commons.wikimedia.org/w/index.php?curid=8952388
Plant cutting "https://commons.wikimedia.org/wiki/File%3AFicus_cuttings_with_roots_in_a_bottle..jpg
By Biusch (Own work) [CC BY-SA 3.0 (https://creativecommons.org/licenses/by-sa/3.0)], via Wikimedia Commons
"
Variegated ficus By Forest & Kim Starr, CC BY 3.0, https://commons.wikimedia.org/w/index.php?curid=6142589
Man carrying out tissue culture "https://commons.wikimedia.org/wiki/File%3AResearch.jpg
By Tatpong Tulyanon (Digital camera) [CC0], via Wikimedia Commons"
Tiny plants from tissue culture "https://commons.wikimedia.org/wiki/File%3APhyscomitrella_growing_on_agar_plates.jpg
By Sabisteb (Anja Martin from the Ralf Reski lab) [CC BY-SA 1.0 (https://creativecommons.org/licenses/by-sa/1.0)], via Wikimedia Commons"
Petunias By Gardenandgreen.co.uk - Own work, CC BY-SA 3.0, https://commons.wikimedia.org/w/index.php?curid=31823028
Music credit:
Deliberate Thought by Kevin MacLeod is licensed under a Creative Commons Attribution licence (https://creativecommons.org/licenses/by/4.0/)
Source: http://incompetech.com/music/royalty-free/?keywords=deliberate+thought
Artist: http://incompetech.com/
I sacrificed a finger to bring you this video. please subscribe
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https://www.instagram.com/plantsinjars_tc/
Supplies List Below!
• Scale (0.01g accuracy): https://amzn.to/3I9fDRM
• pH Meter (0.01 pH accuracy): https://amzn.to/41CqJFA
• pH Up & Down: https://amzn.to/3pAM19r
• Spray Bottle: https://amzn.to/3pI3Q6g
• Plastic Gloves: https://amzn.to/41CBBmW
• Forceps: https://amzn.to/42K3VFa
• Scissors: https://amzn.to/3I9dwgK
• Sterile Petri Dishes: https://amzn.to/3nPNHLT
• mL Droppers: https://amzn.to/44X8cXl
• Agar: https://bit.ly/3o4yPcu
• Murashige & Skoog: https://bit.ly/3pMpAOr
• Kinetin: https://bit.ly/41IpXaa
• Polypropylene Containers: https://bit.ly/3pJrlMF
• Light Meter: https://amzn.to/42B1THI
Protocol for video: https://www.researchgate.net/publication/257148024_Micropropagation_of_self-heading_Philodendron_via_direct_shoot_regeneration
(I am aware that Philodendron Mamei is not technically a "self heading philodendron", but this protocol has worked well for me before for other similar philos!)
Timestamps:
• 1:31 Building a still air box
• 3:18 Tissue culture supplies
• 4:45 What is a tissue culture protocol?
• 5:03 Where to find tissue culture protocols
• 5:44 What is tissue culture media?
• 8:55 How to read a tissue culture protocol
• 11:00 Making tissue culture media
• 13:52 Sterilizing the media
• 17:19 Prepping the still air box
• 18:30 Preparing the explants (tissue samples)
• 19:39 Putting the explant into tissue culture
• 25:30 Conditions for growth
• 26:19 When to subculture
Please note that some of the links in the description are affiliate links, which means that if you click on one of the product links and make a purchase, I may receive a commission. This commission comes at no additional cost to you.
Learn about the Perfect Plants proprietary tissue culture practise, why tissue culture is essential to the successful cultivation of any plant on a commercial scale, and how Perfect Plants will help protect the future growth of the cannabis industry.
The technique of tissue culture is a very efficient tool to propagate better improved crops. Cells and tissues of plants are grown invitro in culture medium and are maintained in aseptic conditions. Gottlieb Haberlandt is known as the father of tissue culture. The property of plant cells to grow into a complete plant is known as Totipotency.
To view other chapter videos please purchase from our site https://www.scholarswing.in
In this episode, Nan from @kanjarin.greens from the last episode share with us the process of tissue culture in a way that is casual and digestible even for beginners. This episode was meant to be part of the last episode but I thought to separate it out on its own as it deserve its own spotlight! Never have I met anyone explain tissue culture so eloquently and in a manner that is interesting and fun! Tissue culture is the future of plant production, and is a process responsible for creating new species, mutations, and hybrids. This is an episode not to be missed!
Nursery instagram: @kanjarin.greens
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Jazz background music made by Rhythm Alley
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Background music are created by me exclusively for this channel
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website: https://www.therareplantshop.co.uk/
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___ My Stuff ___
These are all plant related products I buy all the time. They are amazon affiliate links which means I do earn a small percentage if you buy any of these products. You do not have to buy any products using these links if you do not wish to support me, that is completely fine! :)
A shortcut to my general Aroid potting mix:
ORCHID BARK: https://amzn.to/2MOsVXS
COIR! : https://amzn.to/33naVtz
PERLITE: https://amzn.to/2YRKlJH
ACTIVATED CHARCOAL: https://amzn.to/2KxlgdO
WORM CASTINGS: https://amzn.to/2YM3lJF
SPHAGNUM MOSS: https://amzn.to/2MRnzLq
Coir (Coconut Husk) SMALL - if you don't need much: https://amzn.to/2PkQoyZ
Coir (Coconut Husk) LARGE: https://amzn.to/2Iw3d9l
Perlite: https://amzn.to/2IryzxX
My Plant Pots: https://amzn.to/2VTAgr1
My moisture probe: https://amzn.to/2NO909X
My Large Humidifier: https://amzn.to/2TpWLq3
My Smaller (Alexa) Humidifier: https://amzn.to/2NQmUZ9
Thermometer/Hygrometer: https://amzn.to/2Hrh4My
My Clip on Grow Lights: https://amzn.to/2DxWWFP
Some really good cheap Elho outer pots I use: https://amzn.to/2GBK3eP
1. Create dynamism in Sugarcane crop activities.
2. Sugarcane grows well even in adverse conditions.
3. Sugarcane produces long and plentiful shoots quickly.
4. Sugarcane shoots thrive and the germination rate increases.
5. Increases absorption of primary, secondary, and micronutrients and increases their intracellular transport.
6. The process of cell division is accelerated, so the leaves become thicker, wider, and longer. As a result, the rate of photosynthesis increases.
7. Increased immunity makes sugarcane less susceptible to diseases and insects.
8. Increases the number of canes per acre, stalk length, and thickness and helps increase yield.
✅ Website👇
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Live. Laugh. Autoclave. Plants are science! Let's propagate them the scientific way.
Find me on Instagram: https://www.instagram.com/plantsinjars_tissueculture/
Where I buy tissue culture supplies: https://www.plantcelltechnology.com/?ref=PLANTSINJARS
Supplies List:
• The BioCoupler: https://bit.ly/3p2bvwj
• Murashige & Skoog: https://bit.ly/3pMpAOr
• Kinetin: https://bit.ly/41IpXaa
• BAP: https://bit.ly/3MYJ2zv
• IBA: https://bit.ly/3N0cghE
• Scale (0.01g accuracy): https://amzn.to/3I9fDRM
• pH Meter: https://amzn.to/3NkTiU6
• pH Up & Down: https://amzn.to/3pAM19r
• Forceps: https://amzn.to/42K3VFa
• mL Droppers: https://amzn.to/44X8cXl
Use code PLANTSINJARS for 10% off your Plant Cell Technology order.
Protocol: https://journals.ashs.org/hortsci/view/journals/hortsci/55/3/article-p294.xml
This protocol uses PPFD to measure light. To convert PPFD to lux, I use this calculator: https://www.waveformlighting.com/horticulture/convert-ppfd-to-lux-online-calculator
Timestamps:
1:51: What is the BioCoupler?
3:09: Why does the BioCoupler work?
4:13: Overview of the Tissue Culture Process
6:19: How to Make Liquid Tissue Culture Media
10:16: How to Sterilize a BioCoupler
12:44: Setting up the Still Air Transfer Box
14:06: Still Air Box Demo
16:49: Laminar Flow Hood Demo
17:40: Bonus Experiment
Please note that some of the links in the description are affiliate links, which means that if you click on one of the product links and make a purchase, I may receive a commission. This commission comes at no additional cost to you.
Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues or organs under sterile conditions on a nutrient culture medium of known composition. Plant tissue culture is widely used to produce clones of a plant in a method known as micropropagation. Different techniques in plant tissue culture may offer certain advantages over traditional methods of propagation, including:
The production of exact copies of plants that produce particularly good flowers, fruits, or have other desirable traits.
To quickly produce mature plants.
The production of multiples of plants in the absence of seeds or necessary pollinators to produce seeds.
The regeneration of whole plants from plant cells that have been genetically modified.
The production of plants in sterile containers that allows them to be moved with greatly reduced chances of transmitting diseases, pests, and pathogens.
The production of plants from seeds that otherwise have very low chances of germinating and growing, i.e.: orchids and Nepenthes.
They are currently engaged in extensive research to produce millions of Stevia plants through tissue culture and supply them across Andhra Pradesh.Stevia (Stevia rebaudiana) is a plant native to Mexico.