The yeast Saccharomyces cerevisiae is a simple single-celledeukaryote with both a diploid and haploid mode of existence. The mating of yeast only occurs between haploids, which can be either the a or α (alpha) mating type and thus display simple sexual differentiation. Mating type is determined by a single locus, MAT, which in turn governs the sexual behaviour of both haploid and diploid cells. Through a form of genetic recombination, haploid yeast can switch mating type as often as every cell cycle.
Mating type and the life cycle of Saccharomyces cerevisiae
S. cerevisiae (yeast) can stably exist as either a diploid or a haploid. Both haploid and diploid yeast cells reproduce by mitosis, with daughter cells budding off of mother cells. Haploid cells are capable of mating with other haploid cells of the opposite mating type (an a cell can only mate with an α cell, and vice versa) to produce a stable diploid cell. Diploid cells, usually upon facing stressful conditions such as nutrient depletion, can undergo meiosis to produce four haploid spores: two a spores and two α spores.
The yeast Saccharomyces cerevisiae is a simple single-celled eukaryote with both a diploid and haploid mode of existence. The mating of yeast only occurs between haploids, which can be either the a or α (alpha) mating type and thus display simple sexual differentiation. Mating type is determined by a single locus, MAT, which in turn governs the sexual behaviour of both haploid and diploid cells. Through a form of genetic recombination, haploid yeast can switch mating type as often as every cell cycle.
a cells produce ‘a-factor’, a mating pheromone which signals the presence of an a cell to neighbouring α cells. a cells respond to α-factor, the α cell mating pheromone, by growing a projection (known as a shmoo, due to its distinctive shape) towards the source of α-factor. Similarly, α cells...
published: 10 Nov 2017
Andrew Murray (Harvard) Part 1: Yeast Sex: An Introduction
https://www.ibiology.org/cell-biology/yeast-life-cycle/
Murray begins his talk by explaining why he studies sex in yeast not humans. He describes the yeast life cycle including the decision to bud in the absence of a mate, or to shmoo and mate in the presence of yeast of the correct mating type. In either case, the cells must switch from uniform to non-uniform or polarized growth. Mating cells must also recognize a chemical signal and move towards a target cell. Murray explains the molecular details known to underlie the response to the chemical signal.
In Part 2, Murray describes experiments done in his lab to learn more about mating in yeast. These experiments provide an example of how models are proposed, how experiments are designed to discriminate between the models and how fu...
published: 08 Feb 2012
Pheromone induced mating of haploid yeast cells
In this video we have discussed about the pheromone induced mating of haploid yeast cells .
published: 16 Sep 2023
Sterile 5's role in the Yeast mating Pathway,
Yeast signal transduction in the mating pathway is a complicated art. Sterile 5 plays a large and enigmatic role in this process. Research from Jeremy Thorner's lab, UC Berkeley. Animatic by Diana Saville
published: 21 Jul 2010
Yeast mating
Video of two Saccharomyces cerevisiae (baker's yeast) mating inside a tetrad. The yeast strain is BY4743 and their are here culture on YPDa media. The timelapse was taken with CoolsnapHQ camera mounted a Zeiss axioplan 2 phase contrast microscope using 63X oil immersion objective
published: 11 Jul 2014
Cell signaling in yeast reproduction
Courses on Khan Academy are always 100% free. Start practicing—and saving your progress—now: https://www.khanacademy.org/science/ap-biology/cell-communication-and-cell-cycle/signal-transduction/v/cell-signaling-in-yeast-reproduction
Cell signaling in yeast reproduction
published: 17 Nov 2015
Diploid and haploid yeast cells/mating of haploid yeast cells (cell signaling)
#biotechnology #cellsignaling #yeast
Haploid yeast cells mate through a process called mating or conjugation. During this process, two haploid yeast cells of opposite mating types, designated as "a" and "alpha," come into contact with each other and form a mating bridge or conjugation tube.
The mating process is initiated by the secretion of pheromones by one of the haploid cells. The pheromones act as chemical signals that attract the other haploid cell of the opposite mating type. The two cells then extend projections toward each other and form a conjugation bridge, which allows them to exchange genetic material.
The nucleus of one of the haploid cells then migrates through the conjugation tube and fuses with the nucleus of the other haploid cell, resulting in the formation of a diplo...
published: 09 Feb 2020
Mating Pheromones in Yeast
Check my animation made with #FlipaClip
Download Free - https://r4x8c.app.goo.gl/avWz
published: 16 Jan 2020
Yeast MAP kinase cascade in the mating pathway
In this video we have discussed about the MAP kinase cascade involved in mating pathway.It occurs in Yeast cell.
The yeast Saccharomyces cerevisiae is a simple single-celled eukaryote with both a diploid and haploid mode of existence. The mating of yeast only occurs betwee...
The yeast Saccharomyces cerevisiae is a simple single-celled eukaryote with both a diploid and haploid mode of existence. The mating of yeast only occurs between haploids, which can be either the a or α (alpha) mating type and thus display simple sexual differentiation. Mating type is determined by a single locus, MAT, which in turn governs the sexual behaviour of both haploid and diploid cells. Through a form of genetic recombination, haploid yeast can switch mating type as often as every cell cycle.
a cells produce ‘a-factor’, a mating pheromone which signals the presence of an a cell to neighbouring α cells. a cells respond to α-factor, the α cell mating pheromone, by growing a projection (known as a shmoo, due to its distinctive shape) towards the source of α-factor. Similarly, α cells produce α-factor, and respond to a-factor by growing a projection towards the source of the pheromone. The response of haploid cells only to the mating pheromones of the opposite mating type allows mating between a and α cells, but not between cells of the same mating type.
Mating in yeast is stimulated by the presence of a pheromone which binds to either the Ste2 receptor (in a-cells) or the Ste3 receptor (in α-cells). The binding of this pheromone then leads to the activation of a heterotrimeric G-protein. The dimeric portion of this G-protein recruits Ste5 (and its related MAPK cascade components) to the membrane, and ultimately results in the phosphorylation of Fus3.
The switching mechanism arises as a result of competition between the Fus3 protein (a MAPK protein) and the phosphatase Ptc1. These proteins both attempt to control the 4 phosphorylation sites of Ste5, a scaffold protein with Fus3 attempting to phosphorylate the phosphosites, and Ptc1 attempting to dephosphorylate them.
Presence of α-factor induces recruitment of Ptc1 to Ste5 via a 4 amino acid motif located within the Ste5 phosphosites. Ptc1 then dephosphorylates Ste5, ultimately resulting in the dissociation of the Fus3-Ste5 complex. Fus3 dissociates in a switch-like manner, dependant on the phosphorylation state of the 4 phosphosites. All 4 phosphosites must be dephosphorylated in order for Fus3 to dissociate. Fus3’s ability to compete with Ptc1 decreases as Ptc1 is recruited, and thus the rate of dephosphorylation increases with the presence of pheromone.
Kss1, a homologue of Fus3, does not affect shmooing, and does not contribute to the switch-like mating decision.
In yeast, mating as well as the production of shmoos occur via an all-or-none, switch-like mechanism. This switch-like mechanism allows yeast cells to avoid making an unwise commitment to a highly demanding procedure. However, not only does the mating decision need to be conservative (in order to avoid wasting energy), but it must also be fast to avoid losing the potential mate.
The decision to mate is extremely sensitive. There are 3 ways in which this ultrasensitivity is maintained:
Multi-site phosphorylation – Fus3 only dissociates from Ste5 and becomes fully active when all 4 of the phosphosites are dephosphorylated. Even one phosphorylated site will result in immunity to α-factor.
Two-stage binding – Fus3 and Ptc1 bind to separate docking sites on Ste5. Only after docking can they bind to, and act on, the phosphosites.
Steric hindrance – competition between Fus3 and Ptc1 to control the 4 phosphosites on Ste3
Note: a and α yeast share the same mating response pathway, with the only difference being the type of receptor each mating type possesses. Thus the above description, given for a-type yeast stimulated with α-factor, works equally well for α-type yeast stimulated with a-factor]
Shmoo projections
The yeast Saccharomyces cerevisiae is a simple single-celled eukaryote with both a diploid and haploid mode of existence. The mating of yeast only occurs between haploids, which can be either the a or α (alpha) mating type and thus display simple sexual differentiation. Mating type is determined by a single locus, MAT, which in turn governs the sexual behaviour of both haploid and diploid cells. Through a form of genetic recombination, haploid yeast can switch mating type as often as every cell cycle.
a cells produce ‘a-factor’, a mating pheromone which signals the presence of an a cell to neighbouring α cells. a cells respond to α-factor, the α cell mating pheromone, by growing a projection (known as a shmoo, due to its distinctive shape) towards the source of α-factor. Similarly, α cells produce α-factor, and respond to a-factor by growing a projection towards the source of the pheromone. The response of haploid cells only to the mating pheromones of the opposite mating type allows mating between a and α cells, but not between cells of the same mating type.
Mating in yeast is stimulated by the presence of a pheromone which binds to either the Ste2 receptor (in a-cells) or the Ste3 receptor (in α-cells). The binding of this pheromone then leads to the activation of a heterotrimeric G-protein. The dimeric portion of this G-protein recruits Ste5 (and its related MAPK cascade components) to the membrane, and ultimately results in the phosphorylation of Fus3.
The switching mechanism arises as a result of competition between the Fus3 protein (a MAPK protein) and the phosphatase Ptc1. These proteins both attempt to control the 4 phosphorylation sites of Ste5, a scaffold protein with Fus3 attempting to phosphorylate the phosphosites, and Ptc1 attempting to dephosphorylate them.
Presence of α-factor induces recruitment of Ptc1 to Ste5 via a 4 amino acid motif located within the Ste5 phosphosites. Ptc1 then dephosphorylates Ste5, ultimately resulting in the dissociation of the Fus3-Ste5 complex. Fus3 dissociates in a switch-like manner, dependant on the phosphorylation state of the 4 phosphosites. All 4 phosphosites must be dephosphorylated in order for Fus3 to dissociate. Fus3’s ability to compete with Ptc1 decreases as Ptc1 is recruited, and thus the rate of dephosphorylation increases with the presence of pheromone.
Kss1, a homologue of Fus3, does not affect shmooing, and does not contribute to the switch-like mating decision.
In yeast, mating as well as the production of shmoos occur via an all-or-none, switch-like mechanism. This switch-like mechanism allows yeast cells to avoid making an unwise commitment to a highly demanding procedure. However, not only does the mating decision need to be conservative (in order to avoid wasting energy), but it must also be fast to avoid losing the potential mate.
The decision to mate is extremely sensitive. There are 3 ways in which this ultrasensitivity is maintained:
Multi-site phosphorylation – Fus3 only dissociates from Ste5 and becomes fully active when all 4 of the phosphosites are dephosphorylated. Even one phosphorylated site will result in immunity to α-factor.
Two-stage binding – Fus3 and Ptc1 bind to separate docking sites on Ste5. Only after docking can they bind to, and act on, the phosphosites.
Steric hindrance – competition between Fus3 and Ptc1 to control the 4 phosphosites on Ste3
Note: a and α yeast share the same mating response pathway, with the only difference being the type of receptor each mating type possesses. Thus the above description, given for a-type yeast stimulated with α-factor, works equally well for α-type yeast stimulated with a-factor]
Shmoo projections
https://www.ibiology.org/cell-biology/yeast-life-cycle/
Murray begins his talk by explaining why he studies sex in yeast not humans. He describes the yeast li...
https://www.ibiology.org/cell-biology/yeast-life-cycle/
Murray begins his talk by explaining why he studies sex in yeast not humans. He describes the yeast life cycle including the decision to bud in the absence of a mate, or to shmoo and mate in the presence of yeast of the correct mating type. In either case, the cells must switch from uniform to non-uniform or polarized growth. Mating cells must also recognize a chemical signal and move towards a target cell. Murray explains the molecular details known to underlie the response to the chemical signal.
In Part 2, Murray describes experiments done in his lab to learn more about mating in yeast. These experiments provide an example of how models are proposed, how experiments are designed to discriminate between the models and how further experiments may be needed to address questions raised by the models.
https://www.ibiology.org/cell-biology/yeast-life-cycle/
Murray begins his talk by explaining why he studies sex in yeast not humans. He describes the yeast life cycle including the decision to bud in the absence of a mate, or to shmoo and mate in the presence of yeast of the correct mating type. In either case, the cells must switch from uniform to non-uniform or polarized growth. Mating cells must also recognize a chemical signal and move towards a target cell. Murray explains the molecular details known to underlie the response to the chemical signal.
In Part 2, Murray describes experiments done in his lab to learn more about mating in yeast. These experiments provide an example of how models are proposed, how experiments are designed to discriminate between the models and how further experiments may be needed to address questions raised by the models.
Yeast signal transduction in the mating pathway is a complicated art. Sterile 5 plays a large and enigmatic role in this process. Research from Jeremy Thorner'...
Yeast signal transduction in the mating pathway is a complicated art. Sterile 5 plays a large and enigmatic role in this process. Research from Jeremy Thorner's lab, UC Berkeley. Animatic by Diana Saville
Yeast signal transduction in the mating pathway is a complicated art. Sterile 5 plays a large and enigmatic role in this process. Research from Jeremy Thorner's lab, UC Berkeley. Animatic by Diana Saville
Video of two Saccharomyces cerevisiae (baker's yeast) mating inside a tetrad. The yeast strain is BY4743 and their are here culture on YPDa media. The timelapse...
Video of two Saccharomyces cerevisiae (baker's yeast) mating inside a tetrad. The yeast strain is BY4743 and their are here culture on YPDa media. The timelapse was taken with CoolsnapHQ camera mounted a Zeiss axioplan 2 phase contrast microscope using 63X oil immersion objective
Video of two Saccharomyces cerevisiae (baker's yeast) mating inside a tetrad. The yeast strain is BY4743 and their are here culture on YPDa media. The timelapse was taken with CoolsnapHQ camera mounted a Zeiss axioplan 2 phase contrast microscope using 63X oil immersion objective
Courses on Khan Academy are always 100% free. Start practicing—and saving your progress—now: https://www.khanacademy.org/science/ap-biology/cell-communication-...
Courses on Khan Academy are always 100% free. Start practicing—and saving your progress—now: https://www.khanacademy.org/science/ap-biology/cell-communication-and-cell-cycle/signal-transduction/v/cell-signaling-in-yeast-reproduction
Cell signaling in yeast reproduction
Courses on Khan Academy are always 100% free. Start practicing—and saving your progress—now: https://www.khanacademy.org/science/ap-biology/cell-communication-and-cell-cycle/signal-transduction/v/cell-signaling-in-yeast-reproduction
Cell signaling in yeast reproduction
#biotechnology #cellsignaling #yeast
Haploid yeast cells mate through a process called mating or conjugation. During this process, two haploid yeast cells of o...
#biotechnology #cellsignaling #yeast
Haploid yeast cells mate through a process called mating or conjugation. During this process, two haploid yeast cells of opposite mating types, designated as "a" and "alpha," come into contact with each other and form a mating bridge or conjugation tube.
The mating process is initiated by the secretion of pheromones by one of the haploid cells. The pheromones act as chemical signals that attract the other haploid cell of the opposite mating type. The two cells then extend projections toward each other and form a conjugation bridge, which allows them to exchange genetic material.
The nucleus of one of the haploid cells then migrates through the conjugation tube and fuses with the nucleus of the other haploid cell, resulting in the formation of a diploid cell. This new cell contains a combination of genetic material from both parent cells and is capable of undergoing cell division and producing genetically diverse offspring.
After the mating process is complete, the diploid cell can undergo meiosis to produce four haploid spores, which can then be dispersed to produce new haploid yeast cells.
#biotechnology #cellsignaling #yeast
Haploid yeast cells mate through a process called mating or conjugation. During this process, two haploid yeast cells of opposite mating types, designated as "a" and "alpha," come into contact with each other and form a mating bridge or conjugation tube.
The mating process is initiated by the secretion of pheromones by one of the haploid cells. The pheromones act as chemical signals that attract the other haploid cell of the opposite mating type. The two cells then extend projections toward each other and form a conjugation bridge, which allows them to exchange genetic material.
The nucleus of one of the haploid cells then migrates through the conjugation tube and fuses with the nucleus of the other haploid cell, resulting in the formation of a diploid cell. This new cell contains a combination of genetic material from both parent cells and is capable of undergoing cell division and producing genetically diverse offspring.
After the mating process is complete, the diploid cell can undergo meiosis to produce four haploid spores, which can then be dispersed to produce new haploid yeast cells.
The yeast Saccharomyces cerevisiae is a simple single-celled eukaryote with both a diploid and haploid mode of existence. The mating of yeast only occurs between haploids, which can be either the a or α (alpha) mating type and thus display simple sexual differentiation. Mating type is determined by a single locus, MAT, which in turn governs the sexual behaviour of both haploid and diploid cells. Through a form of genetic recombination, haploid yeast can switch mating type as often as every cell cycle.
a cells produce ‘a-factor’, a mating pheromone which signals the presence of an a cell to neighbouring α cells. a cells respond to α-factor, the α cell mating pheromone, by growing a projection (known as a shmoo, due to its distinctive shape) towards the source of α-factor. Similarly, α cells produce α-factor, and respond to a-factor by growing a projection towards the source of the pheromone. The response of haploid cells only to the mating pheromones of the opposite mating type allows mating between a and α cells, but not between cells of the same mating type.
Mating in yeast is stimulated by the presence of a pheromone which binds to either the Ste2 receptor (in a-cells) or the Ste3 receptor (in α-cells). The binding of this pheromone then leads to the activation of a heterotrimeric G-protein. The dimeric portion of this G-protein recruits Ste5 (and its related MAPK cascade components) to the membrane, and ultimately results in the phosphorylation of Fus3.
The switching mechanism arises as a result of competition between the Fus3 protein (a MAPK protein) and the phosphatase Ptc1. These proteins both attempt to control the 4 phosphorylation sites of Ste5, a scaffold protein with Fus3 attempting to phosphorylate the phosphosites, and Ptc1 attempting to dephosphorylate them.
Presence of α-factor induces recruitment of Ptc1 to Ste5 via a 4 amino acid motif located within the Ste5 phosphosites. Ptc1 then dephosphorylates Ste5, ultimately resulting in the dissociation of the Fus3-Ste5 complex. Fus3 dissociates in a switch-like manner, dependant on the phosphorylation state of the 4 phosphosites. All 4 phosphosites must be dephosphorylated in order for Fus3 to dissociate. Fus3’s ability to compete with Ptc1 decreases as Ptc1 is recruited, and thus the rate of dephosphorylation increases with the presence of pheromone.
Kss1, a homologue of Fus3, does not affect shmooing, and does not contribute to the switch-like mating decision.
In yeast, mating as well as the production of shmoos occur via an all-or-none, switch-like mechanism. This switch-like mechanism allows yeast cells to avoid making an unwise commitment to a highly demanding procedure. However, not only does the mating decision need to be conservative (in order to avoid wasting energy), but it must also be fast to avoid losing the potential mate.
The decision to mate is extremely sensitive. There are 3 ways in which this ultrasensitivity is maintained:
Multi-site phosphorylation – Fus3 only dissociates from Ste5 and becomes fully active when all 4 of the phosphosites are dephosphorylated. Even one phosphorylated site will result in immunity to α-factor.
Two-stage binding – Fus3 and Ptc1 bind to separate docking sites on Ste5. Only after docking can they bind to, and act on, the phosphosites.
Steric hindrance – competition between Fus3 and Ptc1 to control the 4 phosphosites on Ste3
Note: a and α yeast share the same mating response pathway, with the only difference being the type of receptor each mating type possesses. Thus the above description, given for a-type yeast stimulated with α-factor, works equally well for α-type yeast stimulated with a-factor]
Shmoo projections
https://www.ibiology.org/cell-biology/yeast-life-cycle/
Murray begins his talk by explaining why he studies sex in yeast not humans. He describes the yeast life cycle including the decision to bud in the absence of a mate, or to shmoo and mate in the presence of yeast of the correct mating type. In either case, the cells must switch from uniform to non-uniform or polarized growth. Mating cells must also recognize a chemical signal and move towards a target cell. Murray explains the molecular details known to underlie the response to the chemical signal.
In Part 2, Murray describes experiments done in his lab to learn more about mating in yeast. These experiments provide an example of how models are proposed, how experiments are designed to discriminate between the models and how further experiments may be needed to address questions raised by the models.
Yeast signal transduction in the mating pathway is a complicated art. Sterile 5 plays a large and enigmatic role in this process. Research from Jeremy Thorner's lab, UC Berkeley. Animatic by Diana Saville
Video of two Saccharomyces cerevisiae (baker's yeast) mating inside a tetrad. The yeast strain is BY4743 and their are here culture on YPDa media. The timelapse was taken with CoolsnapHQ camera mounted a Zeiss axioplan 2 phase contrast microscope using 63X oil immersion objective
Courses on Khan Academy are always 100% free. Start practicing—and saving your progress—now: https://www.khanacademy.org/science/ap-biology/cell-communication-and-cell-cycle/signal-transduction/v/cell-signaling-in-yeast-reproduction
Cell signaling in yeast reproduction
#biotechnology #cellsignaling #yeast
Haploid yeast cells mate through a process called mating or conjugation. During this process, two haploid yeast cells of opposite mating types, designated as "a" and "alpha," come into contact with each other and form a mating bridge or conjugation tube.
The mating process is initiated by the secretion of pheromones by one of the haploid cells. The pheromones act as chemical signals that attract the other haploid cell of the opposite mating type. The two cells then extend projections toward each other and form a conjugation bridge, which allows them to exchange genetic material.
The nucleus of one of the haploid cells then migrates through the conjugation tube and fuses with the nucleus of the other haploid cell, resulting in the formation of a diploid cell. This new cell contains a combination of genetic material from both parent cells and is capable of undergoing cell division and producing genetically diverse offspring.
After the mating process is complete, the diploid cell can undergo meiosis to produce four haploid spores, which can then be dispersed to produce new haploid yeast cells.
The yeast Saccharomyces cerevisiae is a simple single-celledeukaryote with both a diploid and haploid mode of existence. The mating of yeast only occurs between haploids, which can be either the a or α (alpha) mating type and thus display simple sexual differentiation. Mating type is determined by a single locus, MAT, which in turn governs the sexual behaviour of both haploid and diploid cells. Through a form of genetic recombination, haploid yeast can switch mating type as often as every cell cycle.
Mating type and the life cycle of Saccharomyces cerevisiae
S. cerevisiae (yeast) can stably exist as either a diploid or a haploid. Both haploid and diploid yeast cells reproduce by mitosis, with daughter cells budding off of mother cells. Haploid cells are capable of mating with other haploid cells of the opposite mating type (an a cell can only mate with an α cell, and vice versa) to produce a stable diploid cell. Diploid cells, usually upon facing stressful conditions such as nutrient depletion, can undergo meiosis to produce four haploid spores: two a spores and two α spores.