- published: 28 Dec 2018
- views: 401
-
remove the playlistIntercalation (biochemistry)
- remove the playlistIntercalation (biochemistry)
Intercalation (biochemistry)
In biochemistry, intercalation is the insertion of molecules between the planar bases of DNA. This process is used as a method for analyzing DNA and it is also the basis of certain kinds of poisoning.
There are several ways molecules (in this case, also known as ligands) can interact with DNA. Ligands may interact with DNA by covalently binding, electrostatically binding, or intercalating. Intercalation occurs when ligands of an appropriate size and chemical nature fit themselves in between base pairs of DNA. These ligands are mostly polycyclic, aromatic, and planar, and therefore often make good nucleic acid stains. Intensively studied DNA intercalators include berberine, ethidium bromide, proflavine, daunomycin, doxorubicin, and thalidomide. DNA intercalators are used in chemotherapeutic treatment to inhibit DNA replication in rapidly growing cancer cells. Examples include doxorubicin (adriamycin) and daunorubicin (both of which are used in treatment of Hodgkin's lymphoma), and dactinomycin (used in Wilm's tumour, Ewing's Sarcoma, rhabdomyosarcoma).
This page contains text from Wikipedia, the Free Encyclopedia - https://wn.com/Intercalation_(biochemistry)
Podcasts:
-
Intercalation (biochemistry) | Wikipedia audio article
This is an audio version of the Wikipedia Article: https://en.wikipedia.org/wiki/Intercalation_(biochemistry) 00:04:22 See also Listening is a more natural way of learning, when compared to reading. Written language only began at around 3200 BC, but spoken language has existed long ago. Learning by listening is a great way to: - increases imagination and understanding - improves your listening skills - improves your own spoken accent - learn while on the move - reduce eye strain Now learn the vast amount of general knowledge available on Wikipedia through audio (audio article). You could even learn subconsciously by playing the audio while you are sleeping! If you are planning to listen a lot, you could try using a bone conduction headphone, or a standard speaker ins...
published: 28 Dec 2018 -
Intermolecular forces between DNA and intercalating drugs
This video describes the intermolecular forces between DNA and an intercalating drug
published: 13 Jun 2020 -
Intercalation (chemistry)
In chemistry, intercalation is the reversible inclusion or insertion of a molecule into compounds with layered structures. Examples are found in graphite intercalation compounds. Many layered solids intercalate guest molecules. A famous example is the intercalation of potassium into graphite. Intercalation expands the van der Waals gap between sheets, which requires energy. Usually this energy is supplied by charge transfer between the guest and the host solid, i.e., redox. Two potassium graphite compounds are KC8 and KC24. Carbon fluoridesx and) are prepared by reaction of fluorine with graphitic carbon. The color is greyish, white, or yellow. The bond between the carbon and fluorine atoms is covalent, thus fluorine is not intercalated. Such materials have been considered as a cathodes in...
published: 16 Dec 2015 -
DNA intercalation | Explained | Simple & Easy | Academics | University | Research |
DNA intercalation explained, simple easy learning, University, Academics. Research. Primarily for grown-up beginner audience. Bachelors. Masters. Undergraduate. Graduate. My channel and my videos are made for knowledge-seeking grown-up beginner viewers and education-seeking college/university students. My videos and my channel are NOT meant to attract children. Everything in my channel and videos is in English language (international). These videos use visual learning technique, which are compilation of knowledge that already exists all over the internet, I compiled them so the viewers can save their time watching these. #DNAintercalation, #nucleicacidstructure, #DNAstructure, #bachelorsmasters, #collegeuniversity, #BiophysicsTutorial,
published: 22 May 2016 -
DNA Intercalation & Spectral Shift | Biophysical Chemistry | Explained & Clarified
DNA Intercalation and Spectral Shift. Biophysical Chemistry. Spectroscopy. Structural Biochemistry. Brief and basic discussion. Get better score in exam. Easy learning. Illustrated animations. Targeted primarily to grown-up audience. University. Researchers. Bachelors. Masters. Undergraduate. Graduate. My channel and my videos are made for knowledge-seeking grown-up viewers and education-seeking college/university students. My videos and my channel are NOT meant to attract children. Everything in my channel and videos are in English language (international). These videos use visual learning technique, which are compilation of knowledge that already exists all over the internet; I compiled all those theories, concepts, definitions, laws, equations, interpretations, etc., so the viewers ca...
published: 13 Feb 2019 -
Topoisomerase 1 and 2
A nice clip i found on the mechanisms of action of topoisomerase 1 and 2
published: 15 Dec 2009 -
DNA major and minor grooves
My teaching project page: Biochemistry Literacy for Kids https://www.biochemistryliteracyforkids.com/ Proteins and other molecules that bind DNA specifically recognize either the major or minor groove. This is a short video that shows the differences between the major and minor grooves. Keep in mind that the chirality of the 1', 3', and 4' carbons of the deoxyribose confer asymmetry to the double helix and give rise to the major and minor groves.
published: 02 Aug 2015 -
DNA Intercalaters Part 1
In this video we will discuss the mechanism of action of DNA intercalaters and how they achieve an antiproliferative effect
published: 05 Dec 2014 -
Nucleic Acids Research : Single-molecule kinetics and footprinting of DNA bis-intercalation...
Single-molecule kinetics and footprinting of DNA bis-intercalation: the paradigmatic case of Thiocoraline. Joan Camunas-Soler et al (2015), Nucleic Acids Research http://dx.doi.org/10.1093/nar/gkv087 DNA bis-intercalators are widely used in molecular biology with applications ranging from DNA imaging to anticancer pharmacology. Two fundamental aspects of these ligands are the lifetime of the bis-intercalated complexes and their sequence selectivity. Here, we perform single-molecule optical tweezers experiments with the peptide Thiocoraline showing, for the first time, that bis-intercalation is driven by a very slow off-rate that steeply decreases with applied force. This feature reveals the existence of a long-lived (minutes) mono-intercalated intermediate that contributes to the extremel...
published: 21 Feb 2015
developed with YouTube
4:37
Intercalation (biochemistry) | Wikipedia audio article
- Order: Reorder
- Duration: 4:37
- Uploaded Date: 28 Dec 2018
- views: 401
This is an audio version of the Wikipedia Article:
https://en.wikipedia.org/wiki/Intercalation_(biochemistry)
00:04:22 See also
Listening is a more ...
This is an audio version of the Wikipedia Article:
https://en.wikipedia.org/wiki/Intercalation_(biochemistry)
00:04:22 See also
Listening is a more natural way of learning, when compared to reading. Written language only began at around 3200 BC, but spoken language has existed long ago.
Learning by listening is a great way to:
- increases imagination and understanding
- improves your listening skills
- improves your own spoken accent
- learn while on the move
- reduce eye strain
Now learn the vast amount of general knowledge available on Wikipedia through audio (audio article). You could even learn subconsciously by playing the audio while you are sleeping! If you are planning to listen a lot, you could try using a bone conduction headphone, or a standard speaker instead of an earphone.
Listen on Google Assistant through Extra Audio:
https://assistant.google.com/services/invoke/uid/0000001a130b3f91
Other Wikipedia audio articles at:
https://www.youtube.com/results?search_query=wikipedia+tts
Upload your own Wikipedia articles through:
https://github.com/nodef/wikipedia-tts
Speaking Rate: 0.8305601965904479
Voice name: en-GB-Wavenet-B
"I cannot teach anybody anything, I can only make them think."
- Socrates
SUMMARY
=======
In biochemistry, intercalation is the insertion of molecules between the planar bases of deoxyribonucleic acid (DNA). This process is used as a method for analyzing DNA and it is also the basis of certain kinds of poisoning.
There are several ways molecules (in this case, also known as ligands) can interact with DNA. Ligands may interact with DNA by covalently binding, electrostatically binding, or intercalating. Intercalation occurs when ligands of an appropriate size and chemical nature fit themselves in between base pairs of DNA. These ligands are mostly polycyclic, aromatic, and planar, and therefore often make good nucleic acid stains. Intensively studied DNA intercalators include berberine, ethidium bromide, proflavine, daunomycin, doxorubicin, and thalidomide. DNA intercalators are used in chemotherapeutic treatment to inhibit DNA replication in rapidly growing cancer cells. Examples include doxorubicin (adriamycin) and daunorubicin (both of which are used in treatment of Hodgkin's lymphoma), and dactinomycin (used in Wilm's tumour, Ewing's Sarcoma, rhabdomyosarcoma).
Metallointercalators are complexes of a metal cation with polycyclic aromatic ligands. The most commonly used metal ion is ruthenium(II), because its complexes are very slow to decompose in the biological environment. Other metallic cations that have been used include rhodium(III) and iridium(III). Typical ligands attached to the metal ion are dipyridine and terpyridine whose planar structure is ideal for intercalation.In order for an intercalator to fit between base pairs, the DNA must dynamically open a space between its base pairs by unwinding. The degree of unwinding varies depending on the intercalator; for example, ethidium cation (the ionic form of ethidium bromide found in aqueous solution) unwinds DNA by about 26°, whereas proflavine unwinds it by about 17°. This unwinding causes the base pairs to separate, or "rise", creating an opening of about 0.34 nm (3.4 Å). This unwinding induces local structural changes to the DNA strand, such as lengthening of the DNA strand or twisting of the base pairs. These structural modifications can lead to functional changes, often to the inhibition of transcription and replication and DNA repair processes, which makes intercalators potent mutagens. For this reason, DNA intercalators are often carcinogenic, such as the exo (but not the endo) 8,9 epoxide of aflatoxin B1 and acridines such as proflavine or quinacrine.
Intercalation as a mechanism of interaction between cationic, planar, polycyclic aromatic systems of the correct size (on the order of a base pair) was first proposed by Leonard Lerman in 1961. One proposed mechanism of intercalation is as follows: In aqueous isotonic solution, the cationic intercalator is attracted electrostatically to the surface of the polyanionic DNA. The ligand displaces a sodium and/or magnesium cation present in the "condensation cloud" of such cations that surrounds DNA (to partially balance the sum of the negative charges carried by each phosphate oxygen), thus forming a weak electrostatic association with the outer surface of DNA. From this position, the ligand diffuses along the surface of the DNA and may slide into the hydrophobic environment found between two base pairs that may transiently "open" to form an intercalation site, allowing the ethidium to move away from the hydrophilic (aqueous) environment surrounding the DNA and into the intercalation site. The base pairs transiently form such openings due to energy absorbed during collisions with solvent molecules.
https://wn.com/Intercalation_(Biochemistry)_|_Wikipedia_Audio_Article
This is an audio version of the Wikipedia Article:
https://en.wikipedia.org/wiki/Intercalation_(biochemistry)
00:04:22 See also
Listening is a more natural way of learning, when compared to reading. Written language only began at around 3200 BC, but spoken language has existed long ago.
Learning by listening is a great way to:
- increases imagination and understanding
- improves your listening skills
- improves your own spoken accent
- learn while on the move
- reduce eye strain
Now learn the vast amount of general knowledge available on Wikipedia through audio (audio article). You could even learn subconsciously by playing the audio while you are sleeping! If you are planning to listen a lot, you could try using a bone conduction headphone, or a standard speaker instead of an earphone.
Listen on Google Assistant through Extra Audio:
https://assistant.google.com/services/invoke/uid/0000001a130b3f91
Other Wikipedia audio articles at:
https://www.youtube.com/results?search_query=wikipedia+tts
Upload your own Wikipedia articles through:
https://github.com/nodef/wikipedia-tts
Speaking Rate: 0.8305601965904479
Voice name: en-GB-Wavenet-B
"I cannot teach anybody anything, I can only make them think."
- Socrates
SUMMARY
=======
In biochemistry, intercalation is the insertion of molecules between the planar bases of deoxyribonucleic acid (DNA). This process is used as a method for analyzing DNA and it is also the basis of certain kinds of poisoning.
There are several ways molecules (in this case, also known as ligands) can interact with DNA. Ligands may interact with DNA by covalently binding, electrostatically binding, or intercalating. Intercalation occurs when ligands of an appropriate size and chemical nature fit themselves in between base pairs of DNA. These ligands are mostly polycyclic, aromatic, and planar, and therefore often make good nucleic acid stains. Intensively studied DNA intercalators include berberine, ethidium bromide, proflavine, daunomycin, doxorubicin, and thalidomide. DNA intercalators are used in chemotherapeutic treatment to inhibit DNA replication in rapidly growing cancer cells. Examples include doxorubicin (adriamycin) and daunorubicin (both of which are used in treatment of Hodgkin's lymphoma), and dactinomycin (used in Wilm's tumour, Ewing's Sarcoma, rhabdomyosarcoma).
Metallointercalators are complexes of a metal cation with polycyclic aromatic ligands. The most commonly used metal ion is ruthenium(II), because its complexes are very slow to decompose in the biological environment. Other metallic cations that have been used include rhodium(III) and iridium(III). Typical ligands attached to the metal ion are dipyridine and terpyridine whose planar structure is ideal for intercalation.In order for an intercalator to fit between base pairs, the DNA must dynamically open a space between its base pairs by unwinding. The degree of unwinding varies depending on the intercalator; for example, ethidium cation (the ionic form of ethidium bromide found in aqueous solution) unwinds DNA by about 26°, whereas proflavine unwinds it by about 17°. This unwinding causes the base pairs to separate, or "rise", creating an opening of about 0.34 nm (3.4 Å). This unwinding induces local structural changes to the DNA strand, such as lengthening of the DNA strand or twisting of the base pairs. These structural modifications can lead to functional changes, often to the inhibition of transcription and replication and DNA repair processes, which makes intercalators potent mutagens. For this reason, DNA intercalators are often carcinogenic, such as the exo (but not the endo) 8,9 epoxide of aflatoxin B1 and acridines such as proflavine or quinacrine.
Intercalation as a mechanism of interaction between cationic, planar, polycyclic aromatic systems of the correct size (on the order of a base pair) was first proposed by Leonard Lerman in 1961. One proposed mechanism of intercalation is as follows: In aqueous isotonic solution, the cationic intercalator is attracted electrostatically to the surface of the polyanionic DNA. The ligand displaces a sodium and/or magnesium cation present in the "condensation cloud" of such cations that surrounds DNA (to partially balance the sum of the negative charges carried by each phosphate oxygen), thus forming a weak electrostatic association with the outer surface of DNA. From this position, the ligand diffuses along the surface of the DNA and may slide into the hydrophobic environment found between two base pairs that may transiently "open" to form an intercalation site, allowing the ethidium to move away from the hydrophilic (aqueous) environment surrounding the DNA and into the intercalation site. The base pairs transiently form such openings due to energy absorbed during collisions with solvent molecules.
8:58
Intermolecular forces between DNA and intercalating drugs
- Order: Reorder
- Duration: 8:58
- Uploaded Date: 13 Jun 2020
- views: 2375
This video describes the intermolecular forces between DNA and an intercalating drug
This video describes the intermolecular forces between DNA and an intercalating drug
https://wn.com/Intermolecular_Forces_Between_Dna_And_Intercalating_Drugs
This video describes the intermolecular forces between DNA and an intercalating drug
- published: 13 Jun 2020
- views: 2375
3:02
Intercalation (chemistry)
- Order: Reorder
- Duration: 3:02
- Uploaded Date: 16 Dec 2015
- views: 18754
In chemistry, intercalation is the reversible inclusion or insertion of a molecule into compounds with layered structures. Examples are found in graphite interc...
In chemistry, intercalation is the reversible inclusion or insertion of a molecule into compounds with layered structures. Examples are found in graphite intercalation compounds.
Many layered solids intercalate guest molecules. A famous example is the intercalation of potassium into graphite. Intercalation expands the van der Waals gap between sheets, which requires energy. Usually this energy is supplied by charge transfer between the guest and the host solid, i.e., redox. Two potassium graphite compounds are KC8 and KC24. Carbon fluoridesx and) are prepared by reaction of fluorine with graphitic carbon. The color is greyish, white, or yellow. The bond between the carbon and fluorine atoms is covalent, thus fluorine is not intercalated. Such materials have been considered as a cathodes in various lithium batteries.
This video is targeted to blind users.
Attribution:
Article text available under CC-BY-SA
Creative Commons image source in video
https://wn.com/Intercalation_(Chemistry)
In chemistry, intercalation is the reversible inclusion or insertion of a molecule into compounds with layered structures. Examples are found in graphite intercalation compounds.
Many layered solids intercalate guest molecules. A famous example is the intercalation of potassium into graphite. Intercalation expands the van der Waals gap between sheets, which requires energy. Usually this energy is supplied by charge transfer between the guest and the host solid, i.e., redox. Two potassium graphite compounds are KC8 and KC24. Carbon fluoridesx and) are prepared by reaction of fluorine with graphitic carbon. The color is greyish, white, or yellow. The bond between the carbon and fluorine atoms is covalent, thus fluorine is not intercalated. Such materials have been considered as a cathodes in various lithium batteries.
This video is targeted to blind users.
Attribution:
Article text available under CC-BY-SA
Creative Commons image source in video
- published: 16 Dec 2015
- views: 18754
3:08
DNA intercalation | Explained | Simple & Easy | Academics | University | Research |
- Order: Reorder
- Duration: 3:08
- Uploaded Date: 22 May 2016
- views: 8962
DNA intercalation explained, simple easy learning, University, Academics. Research. Primarily for grown-up beginner audience.
Bachelors. Masters. Undergraduate....
DNA intercalation explained, simple easy learning, University, Academics. Research. Primarily for grown-up beginner audience.
Bachelors. Masters. Undergraduate. Graduate.
My channel and my videos are made for knowledge-seeking grown-up beginner viewers and education-seeking college/university students. My videos and my channel are NOT meant to attract children. Everything in my channel and videos is in English language (international).
These videos use visual learning technique, which are compilation of knowledge that already exists all over the internet, I compiled them so the viewers can save their time watching these.
#DNAintercalation, #nucleicacidstructure, #DNAstructure, #bachelorsmasters, #collegeuniversity, #BiophysicsTutorial,
https://wn.com/Dna_Intercalation_|_Explained_|_Simple_Easy_|_Academics_|_University_|_Research_|
DNA intercalation explained, simple easy learning, University, Academics. Research. Primarily for grown-up beginner audience.
Bachelors. Masters. Undergraduate. Graduate.
My channel and my videos are made for knowledge-seeking grown-up beginner viewers and education-seeking college/university students. My videos and my channel are NOT meant to attract children. Everything in my channel and videos is in English language (international).
These videos use visual learning technique, which are compilation of knowledge that already exists all over the internet, I compiled them so the viewers can save their time watching these.
#DNAintercalation, #nucleicacidstructure, #DNAstructure, #bachelorsmasters, #collegeuniversity, #BiophysicsTutorial,
- published: 22 May 2016
- views: 8962
1:34
DNA Intercalation & Spectral Shift | Biophysical Chemistry | Explained & Clarified
- Order: Reorder
- Duration: 1:34
- Uploaded Date: 13 Feb 2019
- views: 1304
DNA Intercalation and Spectral Shift. Biophysical Chemistry. Spectroscopy. Structural Biochemistry. Brief and basic discussion. Get better score in exam. Easy l...
DNA Intercalation and Spectral Shift. Biophysical Chemistry. Spectroscopy. Structural Biochemistry. Brief and basic discussion. Get better score in exam. Easy learning. Illustrated animations.
Targeted primarily to grown-up audience. University. Researchers.
Bachelors. Masters. Undergraduate. Graduate.
My channel and my videos are made for knowledge-seeking grown-up viewers and education-seeking college/university students. My videos and my channel are NOT meant to attract children. Everything in my channel and videos are in English language (international).
These videos use visual learning technique, which are compilation of knowledge that already exists all over the internet; I compiled all those theories, concepts, definitions, laws, equations, interpretations, etc., so the viewers can save their time watching these. So,……… relax, sit down and watch my videos, learn by heart and go to the exams with your heads high. No worries.
isosbestic point, isosbestic plot, wavelength, spectroscopy, electronic transition, spectra, radiation, electron, light, excited state, ground state, anti-bonding, nucleic acid, DNA, adenine, guanine, thymine, cytosine, hypochromic, hypsochromic, hyperchromic, bathochromic, base-stacking, dipole, chromophore, sigmoidal curve, folded structure, denaturation, polar, orbital, intercalation, coupling, frequency, absorbance, chemical process, reactant, product, inter-conversion, concentration, chemical species, extinction coefficient, Beer-Lambert, stoichiometry, equilibrium, quadruplex, acid-base indicator, oxidation, reduction, chemical kinetics, oximetry, clinical chemistry,
Spectrometry lab https://youtu.be/ROHNqkgRjrI
Electronic Transitions https://youtu.be/aiwkVJQNwXo
Polarity and Spectral Changes https://youtu.be/X-eeU1NRn94
Spectral Shifts in DNA Spectroscopy https://youtu.be/1NDX_xU4azY
DNA Intercalation & Spectral Shift https://youtu.be/NRYhVRHSUaI
Isosbestic Point in Spectroscopy https://youtu.be/mujMHyG7fSc
Isosbestic Point in Chemical Reaction (2 species) https://youtu.be/dT0sxvZII7I
Isosbestic Point in Chemical Reaction (more than 2 species) https://youtu.be/UknwNlegW_Y
Isosbestic Point in Interconversion https://youtu.be/_EaCn_IAm7I
#nucleicacidbiophysics, #dnaspectroscopy, #biophysicsresearch, #opticalspectroscopy, #tutorialbiophysics,
https://wn.com/Dna_Intercalation_Spectral_Shift_|_Biophysical_Chemistry_|_Explained_Clarified
DNA Intercalation and Spectral Shift. Biophysical Chemistry. Spectroscopy. Structural Biochemistry. Brief and basic discussion. Get better score in exam. Easy learning. Illustrated animations.
Targeted primarily to grown-up audience. University. Researchers.
Bachelors. Masters. Undergraduate. Graduate.
My channel and my videos are made for knowledge-seeking grown-up viewers and education-seeking college/university students. My videos and my channel are NOT meant to attract children. Everything in my channel and videos are in English language (international).
These videos use visual learning technique, which are compilation of knowledge that already exists all over the internet; I compiled all those theories, concepts, definitions, laws, equations, interpretations, etc., so the viewers can save their time watching these. So,……… relax, sit down and watch my videos, learn by heart and go to the exams with your heads high. No worries.
isosbestic point, isosbestic plot, wavelength, spectroscopy, electronic transition, spectra, radiation, electron, light, excited state, ground state, anti-bonding, nucleic acid, DNA, adenine, guanine, thymine, cytosine, hypochromic, hypsochromic, hyperchromic, bathochromic, base-stacking, dipole, chromophore, sigmoidal curve, folded structure, denaturation, polar, orbital, intercalation, coupling, frequency, absorbance, chemical process, reactant, product, inter-conversion, concentration, chemical species, extinction coefficient, Beer-Lambert, stoichiometry, equilibrium, quadruplex, acid-base indicator, oxidation, reduction, chemical kinetics, oximetry, clinical chemistry,
Spectrometry lab https://youtu.be/ROHNqkgRjrI
Electronic Transitions https://youtu.be/aiwkVJQNwXo
Polarity and Spectral Changes https://youtu.be/X-eeU1NRn94
Spectral Shifts in DNA Spectroscopy https://youtu.be/1NDX_xU4azY
DNA Intercalation & Spectral Shift https://youtu.be/NRYhVRHSUaI
Isosbestic Point in Spectroscopy https://youtu.be/mujMHyG7fSc
Isosbestic Point in Chemical Reaction (2 species) https://youtu.be/dT0sxvZII7I
Isosbestic Point in Chemical Reaction (more than 2 species) https://youtu.be/UknwNlegW_Y
Isosbestic Point in Interconversion https://youtu.be/_EaCn_IAm7I
#nucleicacidbiophysics, #dnaspectroscopy, #biophysicsresearch, #opticalspectroscopy, #tutorialbiophysics,
- published: 13 Feb 2019
- views: 1304
1:46
Topoisomerase 1 and 2
- Order: Reorder
- Duration: 1:46
- Uploaded Date: 15 Dec 2009
- views: 989151
A nice clip i found on the mechanisms of action of topoisomerase 1 and 2
A nice clip i found on the mechanisms of action of topoisomerase 1 and 2
https://wn.com/Topoisomerase_1_And_2
A nice clip i found on the mechanisms of action of topoisomerase 1 and 2
- published: 15 Dec 2009
- views: 989151
1:41
DNA major and minor grooves
- Order: Reorder
- Duration: 1:41
- Uploaded Date: 02 Aug 2015
- views: 192470
My teaching project page: Biochemistry Literacy for Kids
https://www.biochemistryliteracyforkids.com/
Proteins and other molecules that bind DNA specifically r...
My teaching project page: Biochemistry Literacy for Kids
https://www.biochemistryliteracyforkids.com/
Proteins and other molecules that bind DNA specifically recognize either the major or minor groove. This is a short video that shows the differences between the major and minor grooves. Keep in mind that the chirality of the 1', 3', and 4' carbons of the deoxyribose confer asymmetry to the double helix and give rise to the major and minor groves.
https://wn.com/Dna_Major_And_Minor_Grooves
My teaching project page: Biochemistry Literacy for Kids
https://www.biochemistryliteracyforkids.com/
Proteins and other molecules that bind DNA specifically recognize either the major or minor groove. This is a short video that shows the differences between the major and minor grooves. Keep in mind that the chirality of the 1', 3', and 4' carbons of the deoxyribose confer asymmetry to the double helix and give rise to the major and minor groves.
- published: 02 Aug 2015
- views: 192470
13:41
DNA Intercalaters Part 1
- Order: Reorder
- Duration: 13:41
- Uploaded Date: 05 Dec 2014
- views: 2534
In this video we will discuss the mechanism of action of DNA intercalaters and how they achieve an antiproliferative effect
In this video we will discuss the mechanism of action of DNA intercalaters and how they achieve an antiproliferative effect
https://wn.com/Dna_Intercalaters_Part_1
In this video we will discuss the mechanism of action of DNA intercalaters and how they achieve an antiproliferative effect
- published: 05 Dec 2014
- views: 2534
0:45
Nucleic Acids Research : Single-molecule kinetics and footprinting of DNA bis-intercalation...
- Order: Reorder
- Duration: 0:45
- Uploaded Date: 21 Feb 2015
- views: 117
Single-molecule kinetics and footprinting of DNA bis-intercalation: the paradigmatic case of Thiocoraline. Joan Camunas-Soler et al (2015), Nucleic Acids Resear...
Single-molecule kinetics and footprinting of DNA bis-intercalation: the paradigmatic case of Thiocoraline. Joan Camunas-Soler et al (2015), Nucleic Acids Research http://dx.doi.org/10.1093/nar/gkv087
DNA bis-intercalators are widely used in molecular biology with applications ranging from DNA imaging to anticancer pharmacology. Two fundamental aspects of these ligands are the lifetime of the bis-intercalated complexes and their sequence selectivity. Here, we perform single-molecule optical tweezers experiments with the peptide Thiocoraline showing, for the first time, that bis-intercalation is driven by a very slow off-rate that steeply decreases with applied force. This feature reveals the existence of a long-lived (minutes) mono-intercalated intermediate that contributes to the extremely long lifetime of the complex (hours). We further exploit this particularly slow kinetics to determine the thermodynamics of binding and persistence length of bis-intercalated DNA for a given fraction of bound ligand, a measurement inaccessible in previous studies of faster intercalating agents. We also develop a novel single-molecule footprinting technique based on DNA unzipping and determine the preferred binding sites of Thiocoraline with one base-pair resolution. This fast and radiolabelling-free footprinting technique provides direct access to the binding sites of small ligands to nucleic acids without the need of cleavage agents. Overall, our results provide new insights into the binding pathway of bis-intercalators and the reported selectivity might be of relevance for this and other anticancer drugs interfering with DNA replication and transcription in carcinogenic cell lines.
Good channel: https://www.youtube.com/Dlium
Subscribe, like and comment.
Good website: https://www.dlium.com
Bookmark, subscribe and comment.
https://wn.com/Nucleic_Acids_Research_Single_Molecule_Kinetics_And_Footprinting_Of_Dna_Bis_Intercalation...
Single-molecule kinetics and footprinting of DNA bis-intercalation: the paradigmatic case of Thiocoraline. Joan Camunas-Soler et al (2015), Nucleic Acids Research http://dx.doi.org/10.1093/nar/gkv087
DNA bis-intercalators are widely used in molecular biology with applications ranging from DNA imaging to anticancer pharmacology. Two fundamental aspects of these ligands are the lifetime of the bis-intercalated complexes and their sequence selectivity. Here, we perform single-molecule optical tweezers experiments with the peptide Thiocoraline showing, for the first time, that bis-intercalation is driven by a very slow off-rate that steeply decreases with applied force. This feature reveals the existence of a long-lived (minutes) mono-intercalated intermediate that contributes to the extremely long lifetime of the complex (hours). We further exploit this particularly slow kinetics to determine the thermodynamics of binding and persistence length of bis-intercalated DNA for a given fraction of bound ligand, a measurement inaccessible in previous studies of faster intercalating agents. We also develop a novel single-molecule footprinting technique based on DNA unzipping and determine the preferred binding sites of Thiocoraline with one base-pair resolution. This fast and radiolabelling-free footprinting technique provides direct access to the binding sites of small ligands to nucleic acids without the need of cleavage agents. Overall, our results provide new insights into the binding pathway of bis-intercalators and the reported selectivity might be of relevance for this and other anticancer drugs interfering with DNA replication and transcription in carcinogenic cell lines.
Good channel: https://www.youtube.com/Dlium
Subscribe, like and comment.
Good website: https://www.dlium.com
Bookmark, subscribe and comment.
- published: 21 Feb 2015
- views: 117
4:37
Intercalation (biochemistry) | Wikipedia audio article
This is an audio version of the Wikipedia Article:
https://en.wikipedia.org/wiki/Intercal...
published: 28 Dec 2018
Intercalation (biochemistry) | Wikipedia audio article
Intercalation (biochemistry) | Wikipedia audio article
- Report rights infringement
- published: 28 Dec 2018
- views: 401
This is an audio version of the Wikipedia Article:
https://en.wikipedia.org/wiki/Intercalation_(biochemistry)
00:04:22 See also
Listening is a more natural way of learning, when compared to reading. Written language only began at around 3200 BC, but spoken language has existed long ago.
Learning by listening is a great way to:
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- Socrates
SUMMARY
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In biochemistry, intercalation is the insertion of molecules between the planar bases of deoxyribonucleic acid (DNA). This process is used as a method for analyzing DNA and it is also the basis of certain kinds of poisoning.
There are several ways molecules (in this case, also known as ligands) can interact with DNA. Ligands may interact with DNA by covalently binding, electrostatically binding, or intercalating. Intercalation occurs when ligands of an appropriate size and chemical nature fit themselves in between base pairs of DNA. These ligands are mostly polycyclic, aromatic, and planar, and therefore often make good nucleic acid stains. Intensively studied DNA intercalators include berberine, ethidium bromide, proflavine, daunomycin, doxorubicin, and thalidomide. DNA intercalators are used in chemotherapeutic treatment to inhibit DNA replication in rapidly growing cancer cells. Examples include doxorubicin (adriamycin) and daunorubicin (both of which are used in treatment of Hodgkin's lymphoma), and dactinomycin (used in Wilm's tumour, Ewing's Sarcoma, rhabdomyosarcoma).
Metallointercalators are complexes of a metal cation with polycyclic aromatic ligands. The most commonly used metal ion is ruthenium(II), because its complexes are very slow to decompose in the biological environment. Other metallic cations that have been used include rhodium(III) and iridium(III). Typical ligands attached to the metal ion are dipyridine and terpyridine whose planar structure is ideal for intercalation.In order for an intercalator to fit between base pairs, the DNA must dynamically open a space between its base pairs by unwinding. The degree of unwinding varies depending on the intercalator; for example, ethidium cation (the ionic form of ethidium bromide found in aqueous solution) unwinds DNA by about 26°, whereas proflavine unwinds it by about 17°. This unwinding causes the base pairs to separate, or "rise", creating an opening of about 0.34 nm (3.4 Å). This unwinding induces local structural changes to the DNA strand, such as lengthening of the DNA strand or twisting of the base pairs. These structural modifications can lead to functional changes, often to the inhibition of transcription and replication and DNA repair processes, which makes intercalators potent mutagens. For this reason, DNA intercalators are often carcinogenic, such as the exo (but not the endo) 8,9 epoxide of aflatoxin B1 and acridines such as proflavine or quinacrine.
Intercalation as a mechanism of interaction between cationic, planar, polycyclic aromatic systems of the correct size (on the order of a base pair) was first proposed by Leonard Lerman in 1961. One proposed mechanism of intercalation is as follows: In aqueous isotonic solution, the cationic intercalator is attracted electrostatically to the surface of the polyanionic DNA. The ligand displaces a sodium and/or magnesium cation present in the "condensation cloud" of such cations that surrounds DNA (to partially balance the sum of the negative charges carried by each phosphate oxygen), thus forming a weak electrostatic association with the outer surface of DNA. From this position, the ligand diffuses along the surface of the DNA and may slide into the hydrophobic environment found between two base pairs that may transiently "open" to form an intercalation site, allowing the ethidium to move away from the hydrophilic (aqueous) environment surrounding the DNA and into the intercalation site. The base pairs transiently form such openings due to energy absorbed during collisions with solvent molecules.
8:58
Intermolecular forces between DNA and intercalating drugs
This video describes the intermolecular forces between DNA and an intercalating drug
published: 13 Jun 2020
Intermolecular forces between DNA and intercalating drugs
Intermolecular forces between DNA and intercalating drugs
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- published: 13 Jun 2020
- views: 2375
This video describes the intermolecular forces between DNA and an intercalating drug
3:02
Intercalation (chemistry)
In chemistry, intercalation is the reversible inclusion or insertion of a molecule into co...
published: 16 Dec 2015
Intercalation (chemistry)
Intercalation (chemistry)
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- published: 16 Dec 2015
- views: 18754
In chemistry, intercalation is the reversible inclusion or insertion of a molecule into compounds with layered structures. Examples are found in graphite intercalation compounds.
Many layered solids intercalate guest molecules. A famous example is the intercalation of potassium into graphite. Intercalation expands the van der Waals gap between sheets, which requires energy. Usually this energy is supplied by charge transfer between the guest and the host solid, i.e., redox. Two potassium graphite compounds are KC8 and KC24. Carbon fluoridesx and) are prepared by reaction of fluorine with graphitic carbon. The color is greyish, white, or yellow. The bond between the carbon and fluorine atoms is covalent, thus fluorine is not intercalated. Such materials have been considered as a cathodes in various lithium batteries.
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3:08
DNA intercalation | Explained | Simple & Easy | Academics | University | Research |
DNA intercalation explained, simple easy learning, University, Academics. Research. Primar...
published: 22 May 2016
DNA intercalation | Explained | Simple & Easy | Academics | University | Research |
DNA intercalation | Explained | Simple & Easy | Academics | University | Research |
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- published: 22 May 2016
- views: 8962
DNA intercalation explained, simple easy learning, University, Academics. Research. Primarily for grown-up beginner audience.
Bachelors. Masters. Undergraduate. Graduate.
My channel and my videos are made for knowledge-seeking grown-up beginner viewers and education-seeking college/university students. My videos and my channel are NOT meant to attract children. Everything in my channel and videos is in English language (international).
These videos use visual learning technique, which are compilation of knowledge that already exists all over the internet, I compiled them so the viewers can save their time watching these.
#DNAintercalation, #nucleicacidstructure, #DNAstructure, #bachelorsmasters, #collegeuniversity, #BiophysicsTutorial,
1:34
DNA Intercalation & Spectral Shift | Biophysical Chemistry | Explained & Clarified
DNA Intercalation and Spectral Shift. Biophysical Chemistry. Spectroscopy. Structural Bioc...
published: 13 Feb 2019
DNA Intercalation & Spectral Shift | Biophysical Chemistry | Explained & Clarified
DNA Intercalation & Spectral Shift | Biophysical Chemistry | Explained & Clarified
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- published: 13 Feb 2019
- views: 1304
DNA Intercalation and Spectral Shift. Biophysical Chemistry. Spectroscopy. Structural Biochemistry. Brief and basic discussion. Get better score in exam. Easy learning. Illustrated animations.
Targeted primarily to grown-up audience. University. Researchers.
Bachelors. Masters. Undergraduate. Graduate.
My channel and my videos are made for knowledge-seeking grown-up viewers and education-seeking college/university students. My videos and my channel are NOT meant to attract children. Everything in my channel and videos are in English language (international).
These videos use visual learning technique, which are compilation of knowledge that already exists all over the internet; I compiled all those theories, concepts, definitions, laws, equations, interpretations, etc., so the viewers can save their time watching these. So,……… relax, sit down and watch my videos, learn by heart and go to the exams with your heads high. No worries.
isosbestic point, isosbestic plot, wavelength, spectroscopy, electronic transition, spectra, radiation, electron, light, excited state, ground state, anti-bonding, nucleic acid, DNA, adenine, guanine, thymine, cytosine, hypochromic, hypsochromic, hyperchromic, bathochromic, base-stacking, dipole, chromophore, sigmoidal curve, folded structure, denaturation, polar, orbital, intercalation, coupling, frequency, absorbance, chemical process, reactant, product, inter-conversion, concentration, chemical species, extinction coefficient, Beer-Lambert, stoichiometry, equilibrium, quadruplex, acid-base indicator, oxidation, reduction, chemical kinetics, oximetry, clinical chemistry,
Spectrometry lab https://youtu.be/ROHNqkgRjrI
Electronic Transitions https://youtu.be/aiwkVJQNwXo
Polarity and Spectral Changes https://youtu.be/X-eeU1NRn94
Spectral Shifts in DNA Spectroscopy https://youtu.be/1NDX_xU4azY
DNA Intercalation & Spectral Shift https://youtu.be/NRYhVRHSUaI
Isosbestic Point in Spectroscopy https://youtu.be/mujMHyG7fSc
Isosbestic Point in Chemical Reaction (2 species) https://youtu.be/dT0sxvZII7I
Isosbestic Point in Chemical Reaction (more than 2 species) https://youtu.be/UknwNlegW_Y
Isosbestic Point in Interconversion https://youtu.be/_EaCn_IAm7I
#nucleicacidbiophysics, #dnaspectroscopy, #biophysicsresearch, #opticalspectroscopy, #tutorialbiophysics,
1:46
Topoisomerase 1 and 2
A nice clip i found on the mechanisms of action of topoisomerase 1 and 2
published: 15 Dec 2009
Topoisomerase 1 and 2
Topoisomerase 1 and 2
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- published: 15 Dec 2009
- views: 989151
A nice clip i found on the mechanisms of action of topoisomerase 1 and 2
1:41
DNA major and minor grooves
My teaching project page: Biochemistry Literacy for Kids
https://www.biochemistryliteracyf...
published: 02 Aug 2015
DNA major and minor grooves
DNA major and minor grooves
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- published: 02 Aug 2015
- views: 192470
My teaching project page: Biochemistry Literacy for Kids
https://www.biochemistryliteracyforkids.com/
Proteins and other molecules that bind DNA specifically recognize either the major or minor groove. This is a short video that shows the differences between the major and minor grooves. Keep in mind that the chirality of the 1', 3', and 4' carbons of the deoxyribose confer asymmetry to the double helix and give rise to the major and minor groves.
13:41
DNA Intercalaters Part 1
In this video we will discuss the mechanism of action of DNA intercalaters and how they ac...
published: 05 Dec 2014
DNA Intercalaters Part 1
DNA Intercalaters Part 1
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- published: 05 Dec 2014
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In this video we will discuss the mechanism of action of DNA intercalaters and how they achieve an antiproliferative effect
0:45
Nucleic Acids Research : Single-molecule kinetics and footprinting of DNA bis-intercalation...
Single-molecule kinetics and footprinting of DNA bis-intercalation: the paradigmatic case ...
published: 21 Feb 2015
Nucleic Acids Research : Single-molecule kinetics and footprinting of DNA bis-intercalation...
Nucleic Acids Research : Single-molecule kinetics and footprinting of DNA bis-intercalation...
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- published: 21 Feb 2015
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Single-molecule kinetics and footprinting of DNA bis-intercalation: the paradigmatic case of Thiocoraline. Joan Camunas-Soler et al (2015), Nucleic Acids Research http://dx.doi.org/10.1093/nar/gkv087
DNA bis-intercalators are widely used in molecular biology with applications ranging from DNA imaging to anticancer pharmacology. Two fundamental aspects of these ligands are the lifetime of the bis-intercalated complexes and their sequence selectivity. Here, we perform single-molecule optical tweezers experiments with the peptide Thiocoraline showing, for the first time, that bis-intercalation is driven by a very slow off-rate that steeply decreases with applied force. This feature reveals the existence of a long-lived (minutes) mono-intercalated intermediate that contributes to the extremely long lifetime of the complex (hours). We further exploit this particularly slow kinetics to determine the thermodynamics of binding and persistence length of bis-intercalated DNA for a given fraction of bound ligand, a measurement inaccessible in previous studies of faster intercalating agents. We also develop a novel single-molecule footprinting technique based on DNA unzipping and determine the preferred binding sites of Thiocoraline with one base-pair resolution. This fast and radiolabelling-free footprinting technique provides direct access to the binding sites of small ligands to nucleic acids without the need of cleavage agents. Overall, our results provide new insights into the binding pathway of bis-intercalators and the reported selectivity might be of relevance for this and other anticancer drugs interfering with DNA replication and transcription in carcinogenic cell lines.
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Intercalation (biochemistry)
In biochemistry, intercalation is the insertion of molecules between the planar bases of DNA. This process is used as a method for analyzing DNA and it is also the basis of certain kinds of poisoning.
There are several ways molecules (in this case, also known as ligands) can interact with DNA. Ligands may interact with DNA by covalently binding, electrostatically binding, or intercalating. Intercalation occurs when ligands of an appropriate size and chemical nature fit themselves in between base pairs of DNA. These ligands are mostly polycyclic, aromatic, and planar, and therefore often make good nucleic acid stains. Intensively studied DNA intercalators include berberine, ethidium bromide, proflavine, daunomycin, doxorubicin, and thalidomide. DNA intercalators are used in chemotherapeutic treatment to inhibit DNA replication in rapidly growing cancer cells. Examples include doxorubicin (adriamycin) and daunorubicin (both of which are used in treatment of Hodgkin's lymphoma), and dactinomycin (used in Wilm's tumour, Ewing's Sarcoma, rhabdomyosarcoma).
This page contains text from Wikipedia, the Free Encyclopedia - https://wn.com/Intercalation_(biochemistry)
Intercalation (biochemistry) | Wikipedia audio art...
Intermolecular forces between DNA and intercalatin...
Intercalation (chemistry)...
DNA intercalation | Explained | Simple & Easy | Ac...
DNA Intercalation & Spectral Shift | Biophysical C...
Topoisomerase 1 and 2...
DNA major and minor grooves...
DNA Intercalaters Part 1...