-
Effect of EDTA on CalModulin 1 (CAM1) Gene Expression and Cell Viability in Tetrahymena thermophila
Eric Hollenbaugh and Riley Galt, Carroll College
The use of metal ion chelators, such as EDTA, is ever present in many of our daily products including shampoo, body soaps, and conditioners. For this project, we wanted to explore how the presence of EDTA influences the viability of Tetrahymena thermophila and expression of the Calmodulin 1 (CAM1) gene. We hypothesized that if EDTA is present in the media, CAM1 expression would increase but Tetrahymena viability would decrease. The CAM1 gene codes for the CalModulin 1 protein which requires calcium for activation and is involved in many cellular processes such as the Phospholipase C signaling pathway and intracellular movement. To test our hypothesis, Tetrahymena cells were cultured either in media containing no EDTA or in a media with 5mM ...
published: 23 Apr 2021
-
Cosmos Briefing: What is Calmodulin?
Three genes known as CALM are identical across all vertebrates, with only one or two variations among all animal species and plants.
For that reason, scientists had thought evolution wanted it to stay unchanged, that it must be essential to the state of being alive.
Then, in 2012, scientists found one family in Sweden possessed a mutation on the CALM1 gene: living and breathing, albeit with shared cardiac issues.
Years later, the genes of convicted murdered Kathleen Folbigg found she too possessed a novel (or unique) variant of the CALM2 gene. A current inquiry in New South Wales is being held to determine whether this gene, passed down to her two daughters, could explain the circumstances of their deaths, for which their mother was found responsible.
But what is the purpose of calmodu...
published: 13 Feb 2023
-
Calmodulin in heart disease (animation)
This scientific animation by Moving Science (www.movingscience.dk) and Helene Halkjær Jensen shows how mutations in the protein Calmodulin can lead to heart disease
published: 20 Feb 2019
-
1 Minute Recap - Smooth Muscle Contraction
How is smooth muscle different to skeletal muscle contraction? What mechanisms assist in smooth muscle contraction? What is calmodulin?
This short and concise video aims to demonstrate the influence of calcium in smooth muscle contraction, how it regulates calmodulin and what happens in when your involuntary muscles contract. Actin and Myosin cross-bridge cycling is also explained. Overall, this forms the final stages of the process called excitation-contraction coupling.
#smoothmuscle #calmodulin #actin #contraction #myosin #calcium #skeletalmuscle #smooth #muscle #muscles
published: 21 Mar 2017
-
Calcium and Calmodulin
Donate here: http://www.aklectures.com/donate.php
Website video: http://www.aklectures.com/lecture/calcium-and-calmodulin
Facebook link: https://www.facebook.com/aklectures
Website link: http://www.aklectures.com
published: 09 May 2015
-
Calmodulin
Video introduction to Calcium Modulated Protein (Calmodulin) produced by Makenzie Duncan. PDB codes 3cLn and 1cfd were used to create this video.
published: 21 Nov 2016
-
Calcium-Calmodulin System | Second messengers | Physiology & Biochemistry
Calcium-Calmodulin System | Second (2nd) messenger system | Smooth muscles Contraction and Relaxation | GI Physiology & Biochemistry.
🚨 Emergency Medicine HighYields Course: https://www.medicosisperfectionalis.com/
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— PREMIUM COURSES not available on YouTube:—
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💦 🐳 Kidney Physiology Course: https://www.medicosisperfectionalis.com/products/
❤️ Cardiac Pharmaco...
published: 06 Dec 2022
-
Calmodulin-1-mCherry and Calmodulin-2-GFP in the yeast cells (S. Pombe)
This movie shows the difference in the dynamics of binding and dissociation of two regulatory proteins: calmodulin-1 (red/mCherry) and calmodulin-2 (green/GFP) during endocytosis in the yeast cells. At the beginning of the event, calmodulin-1 molecules start to accumulate at the site of endocytosis - red spot appears and increases in intensity, which is followed by the accumulation of calmodulin-2 - spot became yellow. After this, the formed vesicle, marked by green calmodulin-2, brakes its contact with plasma membrane and starts to move in cytoplasm leavin red calmodulin-1 at the former site of endocytosis. See full details of this study in https://elifesciences.org/articles/51150
published: 02 Jan 2020
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Reading calcium to write memories: the importance of CaMKII autophosphorylation
Jui-Yun Chang, Paula Parra-Bueno, Tal Laviv, Erzsebet M. Szatmari, Seok-Jin R. Lee, and Ryohei Yasuda. Neuron.
http://dx.doi.org/10.1016/j.neuron.2017.04.041
published: 17 May 2017
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Introduction to Calmodulin
Introduction to Calmodulin
published: 22 Aug 2013
7:14
Effect of EDTA on CalModulin 1 (CAM1) Gene Expression and Cell Viability in Tetrahymena thermophila
Eric Hollenbaugh and Riley Galt, Carroll College
The use of metal ion chelators, such as EDTA, is ever present in many of our daily products including shampoo,...
Eric Hollenbaugh and Riley Galt, Carroll College
The use of metal ion chelators, such as EDTA, is ever present in many of our daily products including shampoo, body soaps, and conditioners. For this project, we wanted to explore how the presence of EDTA influences the viability of Tetrahymena thermophila and expression of the Calmodulin 1 (CAM1) gene. We hypothesized that if EDTA is present in the media, CAM1 expression would increase but Tetrahymena viability would decrease. The CAM1 gene codes for the CalModulin 1 protein which requires calcium for activation and is involved in many cellular processes such as the Phospholipase C signaling pathway and intracellular movement. To test our hypothesis, Tetrahymena cells were cultured either in media containing no EDTA or in a media with 5mM EDTA for 24 hours. During the 24-hour treatment period, Tetrahymena cells from both groups were monitored. The cell viability was measured using a hemocytometer four different times throughout the 24-hour growth period. Immediately following the 24 hours, RNA extraction and Reverse Transcription quantitative-Polymerase Chain Reaction (RT-qPCR) were performed to measure CAM1 gene expression. The presence of EDTA was predicted to increase CAM1 gene expression and decrease cell viability due to the calcium sequestering properties of EDTA, which would decrease the amount of calcium available for CalModulin 1 activation.
https://wn.com/Effect_Of_Edta_On_Calmodulin_1_(Cam1)_Gene_Expression_And_Cell_Viability_In_Tetrahymena_Thermophila
Eric Hollenbaugh and Riley Galt, Carroll College
The use of metal ion chelators, such as EDTA, is ever present in many of our daily products including shampoo, body soaps, and conditioners. For this project, we wanted to explore how the presence of EDTA influences the viability of Tetrahymena thermophila and expression of the Calmodulin 1 (CAM1) gene. We hypothesized that if EDTA is present in the media, CAM1 expression would increase but Tetrahymena viability would decrease. The CAM1 gene codes for the CalModulin 1 protein which requires calcium for activation and is involved in many cellular processes such as the Phospholipase C signaling pathway and intracellular movement. To test our hypothesis, Tetrahymena cells were cultured either in media containing no EDTA or in a media with 5mM EDTA for 24 hours. During the 24-hour treatment period, Tetrahymena cells from both groups were monitored. The cell viability was measured using a hemocytometer four different times throughout the 24-hour growth period. Immediately following the 24 hours, RNA extraction and Reverse Transcription quantitative-Polymerase Chain Reaction (RT-qPCR) were performed to measure CAM1 gene expression. The presence of EDTA was predicted to increase CAM1 gene expression and decrease cell viability due to the calcium sequestering properties of EDTA, which would decrease the amount of calcium available for CalModulin 1 activation.
- published: 23 Apr 2021
- views: 111
5:37
Cosmos Briefing: What is Calmodulin?
Three genes known as CALM are identical across all vertebrates, with only one or two variations among all animal species and plants.
For that reason, scientist...
Three genes known as CALM are identical across all vertebrates, with only one or two variations among all animal species and plants.
For that reason, scientists had thought evolution wanted it to stay unchanged, that it must be essential to the state of being alive.
Then, in 2012, scientists found one family in Sweden possessed a mutation on the CALM1 gene: living and breathing, albeit with shared cardiac issues.
Years later, the genes of convicted murdered Kathleen Folbigg found she too possessed a novel (or unique) variant of the CALM2 gene. A current inquiry in New South Wales is being held to determine whether this gene, passed down to her two daughters, could explain the circumstances of their deaths, for which their mother was found responsible.
But what is the purpose of calmodulin? And why is it so important to human life? Cosmos journalist Matthew Agius explains.
SUBSCRIBE to our YouTube channel: https://bit.ly/RiAusYoutube
---
ABOUT
The Royal Institution of Australia is an independent charity dedicated to connecting people with the world of science through news, videos, events and educational resources.
Cosmos is all about aiming to inspire curiosity in “The Science of Everything” and make the world of science accessible to everyone.
----
Want to inject more science in your life?
Sign up to our newsletter: https://cosmosmagazine.com/newsletter
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https://wn.com/Cosmos_Briefing_What_Is_Calmodulin
Three genes known as CALM are identical across all vertebrates, with only one or two variations among all animal species and plants.
For that reason, scientists had thought evolution wanted it to stay unchanged, that it must be essential to the state of being alive.
Then, in 2012, scientists found one family in Sweden possessed a mutation on the CALM1 gene: living and breathing, albeit with shared cardiac issues.
Years later, the genes of convicted murdered Kathleen Folbigg found she too possessed a novel (or unique) variant of the CALM2 gene. A current inquiry in New South Wales is being held to determine whether this gene, passed down to her two daughters, could explain the circumstances of their deaths, for which their mother was found responsible.
But what is the purpose of calmodulin? And why is it so important to human life? Cosmos journalist Matthew Agius explains.
SUBSCRIBE to our YouTube channel: https://bit.ly/RiAusYoutube
---
ABOUT
The Royal Institution of Australia is an independent charity dedicated to connecting people with the world of science through news, videos, events and educational resources.
Cosmos is all about aiming to inspire curiosity in “The Science of Everything” and make the world of science accessible to everyone.
----
Want to inject more science in your life?
Sign up to our newsletter: https://cosmosmagazine.com/newsletter
Join our community on Facebook: http://fb.me/cosmosmagazine
Follow us on Twitter: https://twitter.com/CosmosMagazine
Follow us on Instagram: https://www.instagram.com/cosmosmagazine/
- published: 13 Feb 2023
- views: 717
1:32
Calmodulin in heart disease (animation)
This scientific animation by Moving Science (www.movingscience.dk) and Helene Halkjær Jensen shows how mutations in the protein Calmodulin can lead to heart dis...
This scientific animation by Moving Science (www.movingscience.dk) and Helene Halkjær Jensen shows how mutations in the protein Calmodulin can lead to heart disease
https://wn.com/Calmodulin_In_Heart_Disease_(Animation)
This scientific animation by Moving Science (www.movingscience.dk) and Helene Halkjær Jensen shows how mutations in the protein Calmodulin can lead to heart disease
- published: 20 Feb 2019
- views: 512
1:39
1 Minute Recap - Smooth Muscle Contraction
How is smooth muscle different to skeletal muscle contraction? What mechanisms assist in smooth muscle contraction? What is calmodulin?
This short and concise ...
How is smooth muscle different to skeletal muscle contraction? What mechanisms assist in smooth muscle contraction? What is calmodulin?
This short and concise video aims to demonstrate the influence of calcium in smooth muscle contraction, how it regulates calmodulin and what happens in when your involuntary muscles contract. Actin and Myosin cross-bridge cycling is also explained. Overall, this forms the final stages of the process called excitation-contraction coupling.
#smoothmuscle #calmodulin #actin #contraction #myosin #calcium #skeletalmuscle #smooth #muscle #muscles
https://wn.com/1_Minute_Recap_Smooth_Muscle_Contraction
How is smooth muscle different to skeletal muscle contraction? What mechanisms assist in smooth muscle contraction? What is calmodulin?
This short and concise video aims to demonstrate the influence of calcium in smooth muscle contraction, how it regulates calmodulin and what happens in when your involuntary muscles contract. Actin and Myosin cross-bridge cycling is also explained. Overall, this forms the final stages of the process called excitation-contraction coupling.
#smoothmuscle #calmodulin #actin #contraction #myosin #calcium #skeletalmuscle #smooth #muscle #muscles
- published: 21 Mar 2017
- views: 170290
11:08
Calcium and Calmodulin
Donate here: http://www.aklectures.com/donate.php
Website video: http://www.aklectures.com/lecture/calcium-and-calmodulin
Facebook link: https://www.facebook.co...
Donate here: http://www.aklectures.com/donate.php
Website video: http://www.aklectures.com/lecture/calcium-and-calmodulin
Facebook link: https://www.facebook.com/aklectures
Website link: http://www.aklectures.com
https://wn.com/Calcium_And_Calmodulin
Donate here: http://www.aklectures.com/donate.php
Website video: http://www.aklectures.com/lecture/calcium-and-calmodulin
Facebook link: https://www.facebook.com/aklectures
Website link: http://www.aklectures.com
- published: 09 May 2015
- views: 106666
9:27
Calmodulin
Video introduction to Calcium Modulated Protein (Calmodulin) produced by Makenzie Duncan. PDB codes 3cLn and 1cfd were used to create this video.
Video introduction to Calcium Modulated Protein (Calmodulin) produced by Makenzie Duncan. PDB codes 3cLn and 1cfd were used to create this video.
https://wn.com/Calmodulin
Video introduction to Calcium Modulated Protein (Calmodulin) produced by Makenzie Duncan. PDB codes 3cLn and 1cfd were used to create this video.
- published: 21 Nov 2016
- views: 10270
5:13
Calcium-Calmodulin System | Second messengers | Physiology & Biochemistry
Calcium-Calmodulin System | Second (2nd) messenger system | Smooth muscles Contraction and Relaxation | GI Physiology & Biochemistry.
🚨 Emergency Medicine High...
Calcium-Calmodulin System | Second (2nd) messenger system | Smooth muscles Contraction and Relaxation | GI Physiology & Biochemistry.
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https://wn.com/Calcium_Calmodulin_System_|_Second_Messengers_|_Physiology_Biochemistry
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--If you have joined my channel on youtube (the join button that's next to the subscribe button), then go to the "community tab" and you will find some member-only posts.
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- published: 06 Dec 2022
- views: 35182
1:00
Calmodulin-1-mCherry and Calmodulin-2-GFP in the yeast cells (S. Pombe)
This movie shows the difference in the dynamics of binding and dissociation of two regulatory proteins: calmodulin-1 (red/mCherry) and calmodulin-2 (green/GFP) ...
This movie shows the difference in the dynamics of binding and dissociation of two regulatory proteins: calmodulin-1 (red/mCherry) and calmodulin-2 (green/GFP) during endocytosis in the yeast cells. At the beginning of the event, calmodulin-1 molecules start to accumulate at the site of endocytosis - red spot appears and increases in intensity, which is followed by the accumulation of calmodulin-2 - spot became yellow. After this, the formed vesicle, marked by green calmodulin-2, brakes its contact with plasma membrane and starts to move in cytoplasm leavin red calmodulin-1 at the former site of endocytosis. See full details of this study in https://elifesciences.org/articles/51150
https://wn.com/Calmodulin_1_Mcherry_And_Calmodulin_2_Gfp_In_The_Yeast_Cells_(S._Pombe)
This movie shows the difference in the dynamics of binding and dissociation of two regulatory proteins: calmodulin-1 (red/mCherry) and calmodulin-2 (green/GFP) during endocytosis in the yeast cells. At the beginning of the event, calmodulin-1 molecules start to accumulate at the site of endocytosis - red spot appears and increases in intensity, which is followed by the accumulation of calmodulin-2 - spot became yellow. After this, the formed vesicle, marked by green calmodulin-2, brakes its contact with plasma membrane and starts to move in cytoplasm leavin red calmodulin-1 at the former site of endocytosis. See full details of this study in https://elifesciences.org/articles/51150
- published: 02 Jan 2020
- views: 81
3:54
Reading calcium to write memories: the importance of CaMKII autophosphorylation
Jui-Yun Chang, Paula Parra-Bueno, Tal Laviv, Erzsebet M. Szatmari, Seok-Jin R. Lee, and Ryohei Yasuda. Neuron.
http://dx.doi.org/10.1016/j.neuron.2017.04.041
Jui-Yun Chang, Paula Parra-Bueno, Tal Laviv, Erzsebet M. Szatmari, Seok-Jin R. Lee, and Ryohei Yasuda. Neuron.
http://dx.doi.org/10.1016/j.neuron.2017.04.041
https://wn.com/Reading_Calcium_To_Write_Memories_The_Importance_Of_Camkii_Autophosphorylation
Jui-Yun Chang, Paula Parra-Bueno, Tal Laviv, Erzsebet M. Szatmari, Seok-Jin R. Lee, and Ryohei Yasuda. Neuron.
http://dx.doi.org/10.1016/j.neuron.2017.04.041
- published: 17 May 2017
- views: 17747