Cardiac muscle cells or cardiomyocytes (also known as myocardiocytes or cardiac myocytes) are the muscle cells (myocytes) that make up the cardiac muscle. Each myocardial cell contains myofibrils, which are specialized organelles consisting of long chains of sarcomeres, the fundamental contractile units of muscle cells. Cardiomyocytes show striations similar to those on skeletal muscle cells. Unlike multinucleated skeletal cells, the majority of cardiomyocytes contain only one nucleus, although they may have as many as four. Cardiomyocytes have a high mitochondrial density, which allows them to produce adenosine triphosphate (ATP) quickly, making them highly resistant to fatigue.
Types of cells
There are two types of cells within the heart: the cardiomyocytes and the cardiac pacemaker cells.
Cardiomyocytes make up the atria (the chambers in which blood enters the heart) and the ventricles (the chambers where blood is collected and pumped out of the heart). These cells must be able to shorten and lengthen their fibers and the fibers must be flexible enough to stretch. These functions are critical to the proper form during the beating of the heart.
Our "how the heart works video" focuses on cardiomyocyte. Your heart beats approximately 100,000 times a day to circulate 2000 gallons of blood. The force exerted is similar to squeezing a tennis ball in your hand every second, 24 hours a day for your entire life. The heart has tremendous endurance.
This video is about the cellular and molecular basis of cardiac pumping. Topics include the electrophysiological basis of the cardiac action potential (which triggers the heart beat), and how that action potential is coupled to calcium signaling, and how calcium triggers contraction.
published: 02 Aug 2019
Cardiomyocyte Cell Cycle & Regeneration
Wuqiang (Wuk) Zhu, MD, PhD, discusses cardiomyocyte cell cycle including cardiomyocyte proliferation and stem cell and myocardial repair.
published: 04 Dec 2018
How to differentiate pluripotent stem cells (PSC) into cardiomyocytes
https://www.thermofisher.com/us/en/home/references/protocols/cell-culture/stem-cell-protocols.html?cid=bid_clb_scl_r01_co_cp1361_pjt5757_bid88888_0vi_yut_vo_awa_kt_s24_SS519
In this video you will see how to use the Gibco PSC Cardiomyocyte Differentiation Kit to generate beating cardiomyocytes from pluripotent stem cells. By using this easy-to-use three-part media system, you can start from a small population of pluripotent stem cells and obtain a large number of beating cardiomyocytes. You can access the product insert and the quick reference protocol online.
A supplemental list of reagents used in this protocol can also be referenced here.
First, prepare a complete Essential 8 Medium. Find details for this preparation on the web link listed below.
Then prepare your substrate by c...
published: 06 Jul 2020
The Cardiomyocyte Action Potential [Part 1]: The Action Potential Graph
In this video, we take a look at the graph representing the cardiomyocyte action potential and what each region of the graph represents.
published: 09 Nov 2019
Time-Lapse: Isolated Murine Cardiomyocyte by Dr. Christopher B. Jackson
2013 Olympus BioScapes International Digital Imaging Competition
Specimen: Isolated living cardiomyocytes from murine (rodent) heart. The cells still have a "heartbeat."
Technique: Polarized light and differential interference contrast
Credit: Dr. Christopher B. Jackson
Prize: Honorable Mention
For more information, visit: Olympus-LifeScience.com
Find and Follow Olympus Life Science on:
Facebook: http://www.facebook.com/pages/Olympus-Life-Science/312676668924012/
Twitter: http://www.twitter.com/@OlympusLifeSci
LinkedIn: https://www.linkedin.com/company/olympus-life-science
Google+: https://plus.google.com/115358294493885697412/about#115358294493885697412/about
Instagram: http://instagram.com/olympuslifescience
published: 30 Aug 2017
Action potentials in cardiac myocytes | Circulatory system physiology | NCLEX-RN | Khan Academy
See how muscle cells in the heart contract by allowing Calcium to flow inside and bringing along some positive charge with it! Rishi is a pediatric infectious disease physician and works at Khan Academy. Created by Rishi Desai.
Watch the next lesson: https://www.khanacademy.org/test-prep/nclex-rn/nclex-rn-circulatory-system/heart-depolarization-ddp/v/reseting-cardiac-concentration-gradients?utm_source=YT&utm_medium=Desc&utm_campaign=Nclex-rn
Missed the previous lesson? https://www.khanacademy.org/test-prep/nclex-rn/nclex-rn-circulatory-system/heart-depolarization-ddp/v/action-potentials-in-pacemaker-cells?utm_source=YT&utm_medium=Desc&utm_campaign=Nclex-rn
NCLEX-RN on Khan Academy: A collection of questions from content covered on the NCLEX-RN. These questions are available under a Cr...
Our "how the heart works video" focuses on cardiomyocyte. Your heart beats approximately 100,000 times a day to circulate 2000 gallons of blood. The force exert...
Our "how the heart works video" focuses on cardiomyocyte. Your heart beats approximately 100,000 times a day to circulate 2000 gallons of blood. The force exerted is similar to squeezing a tennis ball in your hand every second, 24 hours a day for your entire life. The heart has tremendous endurance.
Our "how the heart works video" focuses on cardiomyocyte. Your heart beats approximately 100,000 times a day to circulate 2000 gallons of blood. The force exerted is similar to squeezing a tennis ball in your hand every second, 24 hours a day for your entire life. The heart has tremendous endurance.
This video is about the cellular and molecular basis of cardiac pumping. Topics include the electrophysiological basis of the cardiac action potential (which tr...
This video is about the cellular and molecular basis of cardiac pumping. Topics include the electrophysiological basis of the cardiac action potential (which triggers the heart beat), and how that action potential is coupled to calcium signaling, and how calcium triggers contraction.
This video is about the cellular and molecular basis of cardiac pumping. Topics include the electrophysiological basis of the cardiac action potential (which triggers the heart beat), and how that action potential is coupled to calcium signaling, and how calcium triggers contraction.
https://www.thermofisher.com/us/en/home/references/protocols/cell-culture/stem-cell-protocols.html?cid=bid_clb_scl_r01_co_cp1361_pjt5757_bid88888_0vi_yut_vo_awa_kt_s24_SS519
In this video you will see how to use the Gibco PSC Cardiomyocyte Differentiation Kit to generate beating cardiomyocytes from pluripotent stem cells. By using this easy-to-use three-part media system, you can start from a small population of pluripotent stem cells and obtain a large number of beating cardiomyocytes. You can access the product insert and the quick reference protocol online.
A supplemental list of reagents used in this protocol can also be referenced here.
First, prepare a complete Essential 8 Medium. Find details for this preparation on the web link listed below.
Then prepare your substrate by coating a 12-well plate with a 1:100 Geltrex solution. And place in an incubator for at least one hour.
Next, prepare a cell recovery solution by adding 250 µm of RevitaCell supplement to 25 mL of Essential 8 Medium.
Let warm to room temperature.
Remove the Geltrex coated plate from the incubator and aspirate the plate.
Add 1 mL of cell recovery solution to each well.
Quickly thaw a vial of cryopreserved pluripotent stem cells in a 37˚C water bath until just a small ice crystal remains.
In the biosafety hood, transfer vial contents to a 15 mL tube and slowly add 10 mL cell recovery solution while swirling the tube.
Centrifuge the cells at a relative centrifugal force of 200 x g for 5 minutes.
After centrifugation, aspirate supernatant and gently flick the tube to dislodge cells.
Resuspend cells by adding 2 mL cell recovery solution dropwise.
Perform a viable cell count and calculate the cell density of the cell solution.
Seed plate with the cell solution to achieve 30 to 70% confluence within 3 to 4 days. The differentiation efficiency of PSCs into cardiomyocytes varies between different PSC lines.
A critical variable for the generation of a robust cardiomyocyte culture is the relative confluence at the onset of differentiation. The suggested confluence range at the time of induction is 30 to 70%, and it is strongly recommended that you perform a confluence range finding study to determine the seeding density needed to achieve the optimal confluence for your PSC line within 3 to 4 days of seeding.
Confirm attachment of cells the following day.
Refeed cells with pre-warmed Essential 8 Medium every day for 3 to 4 days to reach target confluency for your PSC line.
Once the plate has achieved target confluency, replace the medium with Cardiomyocyte Differentiation Medium A. Note that the addition of medium should be done slowly and along the sides of the well, as forceful addition of medium will disrupt the differentiation process.
Two days after Cardiomyocyte Differentiation Medium A induction, replace the medium with Cardiomyocyte Differentiation Medium B.
Two days later, replace the medium with Cardiomyocyte Maintenance Medium.
Continue to refeed cells with this medium every other day.
10 days after differentiation has begun using the PSC Cardiomyocyte Differentiation Kit you should observe beating syncytia of cardiomyocytes. These cells are now ready for further studies.
#pscdifferentiation, #stemcelldifferentiation, #cardiomyocytedifferentiation, #differentiatingpluripotentstemcells, #PSCCardiomyocyteDifferentiationKit #thermofisherscientific
https://www.thermofisher.com/us/en/home/references/protocols/cell-culture/stem-cell-protocols.html?cid=bid_clb_scl_r01_co_cp1361_pjt5757_bid88888_0vi_yut_vo_awa_kt_s24_SS519
In this video you will see how to use the Gibco PSC Cardiomyocyte Differentiation Kit to generate beating cardiomyocytes from pluripotent stem cells. By using this easy-to-use three-part media system, you can start from a small population of pluripotent stem cells and obtain a large number of beating cardiomyocytes. You can access the product insert and the quick reference protocol online.
A supplemental list of reagents used in this protocol can also be referenced here.
First, prepare a complete Essential 8 Medium. Find details for this preparation on the web link listed below.
Then prepare your substrate by coating a 12-well plate with a 1:100 Geltrex solution. And place in an incubator for at least one hour.
Next, prepare a cell recovery solution by adding 250 µm of RevitaCell supplement to 25 mL of Essential 8 Medium.
Let warm to room temperature.
Remove the Geltrex coated plate from the incubator and aspirate the plate.
Add 1 mL of cell recovery solution to each well.
Quickly thaw a vial of cryopreserved pluripotent stem cells in a 37˚C water bath until just a small ice crystal remains.
In the biosafety hood, transfer vial contents to a 15 mL tube and slowly add 10 mL cell recovery solution while swirling the tube.
Centrifuge the cells at a relative centrifugal force of 200 x g for 5 minutes.
After centrifugation, aspirate supernatant and gently flick the tube to dislodge cells.
Resuspend cells by adding 2 mL cell recovery solution dropwise.
Perform a viable cell count and calculate the cell density of the cell solution.
Seed plate with the cell solution to achieve 30 to 70% confluence within 3 to 4 days. The differentiation efficiency of PSCs into cardiomyocytes varies between different PSC lines.
A critical variable for the generation of a robust cardiomyocyte culture is the relative confluence at the onset of differentiation. The suggested confluence range at the time of induction is 30 to 70%, and it is strongly recommended that you perform a confluence range finding study to determine the seeding density needed to achieve the optimal confluence for your PSC line within 3 to 4 days of seeding.
Confirm attachment of cells the following day.
Refeed cells with pre-warmed Essential 8 Medium every day for 3 to 4 days to reach target confluency for your PSC line.
Once the plate has achieved target confluency, replace the medium with Cardiomyocyte Differentiation Medium A. Note that the addition of medium should be done slowly and along the sides of the well, as forceful addition of medium will disrupt the differentiation process.
Two days after Cardiomyocyte Differentiation Medium A induction, replace the medium with Cardiomyocyte Differentiation Medium B.
Two days later, replace the medium with Cardiomyocyte Maintenance Medium.
Continue to refeed cells with this medium every other day.
10 days after differentiation has begun using the PSC Cardiomyocyte Differentiation Kit you should observe beating syncytia of cardiomyocytes. These cells are now ready for further studies.
#pscdifferentiation, #stemcelldifferentiation, #cardiomyocytedifferentiation, #differentiatingpluripotentstemcells, #PSCCardiomyocyteDifferentiationKit #thermofisherscientific
2013 Olympus BioScapes International Digital Imaging Competition
Specimen: Isolated living cardiomyocytes from murine (rodent) heart. The cells still have a "h...
2013 Olympus BioScapes International Digital Imaging Competition
Specimen: Isolated living cardiomyocytes from murine (rodent) heart. The cells still have a "heartbeat."
Technique: Polarized light and differential interference contrast
Credit: Dr. Christopher B. Jackson
Prize: Honorable Mention
For more information, visit: Olympus-LifeScience.com
Find and Follow Olympus Life Science on:
Facebook: http://www.facebook.com/pages/Olympus-Life-Science/312676668924012/
Twitter: http://www.twitter.com/@OlympusLifeSci
LinkedIn: https://www.linkedin.com/company/olympus-life-science
Google+: https://plus.google.com/115358294493885697412/about#115358294493885697412/about
Instagram: http://instagram.com/olympuslifescience
2013 Olympus BioScapes International Digital Imaging Competition
Specimen: Isolated living cardiomyocytes from murine (rodent) heart. The cells still have a "heartbeat."
Technique: Polarized light and differential interference contrast
Credit: Dr. Christopher B. Jackson
Prize: Honorable Mention
For more information, visit: Olympus-LifeScience.com
Find and Follow Olympus Life Science on:
Facebook: http://www.facebook.com/pages/Olympus-Life-Science/312676668924012/
Twitter: http://www.twitter.com/@OlympusLifeSci
LinkedIn: https://www.linkedin.com/company/olympus-life-science
Google+: https://plus.google.com/115358294493885697412/about#115358294493885697412/about
Instagram: http://instagram.com/olympuslifescience
See how muscle cells in the heart contract by allowing Calcium to flow inside and bringing along some positive charge with it! Rishi is a pediatric infectious d...
See how muscle cells in the heart contract by allowing Calcium to flow inside and bringing along some positive charge with it! Rishi is a pediatric infectious disease physician and works at Khan Academy. Created by Rishi Desai.
Watch the next lesson: https://www.khanacademy.org/test-prep/nclex-rn/nclex-rn-circulatory-system/heart-depolarization-ddp/v/reseting-cardiac-concentration-gradients?utm_source=YT&utm_medium=Desc&utm_campaign=Nclex-rn
Missed the previous lesson? https://www.khanacademy.org/test-prep/nclex-rn/nclex-rn-circulatory-system/heart-depolarization-ddp/v/action-potentials-in-pacemaker-cells?utm_source=YT&utm_medium=Desc&utm_campaign=Nclex-rn
NCLEX-RN on Khan Academy: A collection of questions from content covered on the NCLEX-RN. These questions are available under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 United States License (available at http://creativecommons.org/licenses/by-nc-sa/3.0/us/).
About Khan Academy: Khan Academy offers practice exercises, instructional videos, and a personalized learning dashboard that empower learners to study at their own pace in and outside of the classroom. We tackle math, science, computer programming, history, art history, economics, and more. Our math missions guide learners from kindergarten to calculus using state-of-the-art, adaptive technology that identifies strengths and learning gaps. We've also partnered with institutions like NASA, The Museum of Modern Art, The California Academy of Sciences, and MIT to offer specialized content.
For free. For everyone. Forever. #YouCanLearnAnything
Subscribe to Khan Academy’s NCLEX-RN channel: https://www.youtube.com/channel/UCDx5cTeADCvKWgF9x_Qjz3g?sub_confirmation=1
Subscribe to Khan Academy: https://www.youtube.com/subscription_center?add_user=khanacademy
See how muscle cells in the heart contract by allowing Calcium to flow inside and bringing along some positive charge with it! Rishi is a pediatric infectious disease physician and works at Khan Academy. Created by Rishi Desai.
Watch the next lesson: https://www.khanacademy.org/test-prep/nclex-rn/nclex-rn-circulatory-system/heart-depolarization-ddp/v/reseting-cardiac-concentration-gradients?utm_source=YT&utm_medium=Desc&utm_campaign=Nclex-rn
Missed the previous lesson? https://www.khanacademy.org/test-prep/nclex-rn/nclex-rn-circulatory-system/heart-depolarization-ddp/v/action-potentials-in-pacemaker-cells?utm_source=YT&utm_medium=Desc&utm_campaign=Nclex-rn
NCLEX-RN on Khan Academy: A collection of questions from content covered on the NCLEX-RN. These questions are available under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 United States License (available at http://creativecommons.org/licenses/by-nc-sa/3.0/us/).
About Khan Academy: Khan Academy offers practice exercises, instructional videos, and a personalized learning dashboard that empower learners to study at their own pace in and outside of the classroom. We tackle math, science, computer programming, history, art history, economics, and more. Our math missions guide learners from kindergarten to calculus using state-of-the-art, adaptive technology that identifies strengths and learning gaps. We've also partnered with institutions like NASA, The Museum of Modern Art, The California Academy of Sciences, and MIT to offer specialized content.
For free. For everyone. Forever. #YouCanLearnAnything
Subscribe to Khan Academy’s NCLEX-RN channel: https://www.youtube.com/channel/UCDx5cTeADCvKWgF9x_Qjz3g?sub_confirmation=1
Subscribe to Khan Academy: https://www.youtube.com/subscription_center?add_user=khanacademy
Our "how the heart works video" focuses on cardiomyocyte. Your heart beats approximately 100,000 times a day to circulate 2000 gallons of blood. The force exerted is similar to squeezing a tennis ball in your hand every second, 24 hours a day for your entire life. The heart has tremendous endurance.
This video is about the cellular and molecular basis of cardiac pumping. Topics include the electrophysiological basis of the cardiac action potential (which triggers the heart beat), and how that action potential is coupled to calcium signaling, and how calcium triggers contraction.
https://www.thermofisher.com/us/en/home/references/protocols/cell-culture/stem-cell-protocols.html?cid=bid_clb_scl_r01_co_cp1361_pjt5757_bid88888_0vi_yut_vo_awa_kt_s24_SS519
In this video you will see how to use the Gibco PSC Cardiomyocyte Differentiation Kit to generate beating cardiomyocytes from pluripotent stem cells. By using this easy-to-use three-part media system, you can start from a small population of pluripotent stem cells and obtain a large number of beating cardiomyocytes. You can access the product insert and the quick reference protocol online.
A supplemental list of reagents used in this protocol can also be referenced here.
First, prepare a complete Essential 8 Medium. Find details for this preparation on the web link listed below.
Then prepare your substrate by coating a 12-well plate with a 1:100 Geltrex solution. And place in an incubator for at least one hour.
Next, prepare a cell recovery solution by adding 250 µm of RevitaCell supplement to 25 mL of Essential 8 Medium.
Let warm to room temperature.
Remove the Geltrex coated plate from the incubator and aspirate the plate.
Add 1 mL of cell recovery solution to each well.
Quickly thaw a vial of cryopreserved pluripotent stem cells in a 37˚C water bath until just a small ice crystal remains.
In the biosafety hood, transfer vial contents to a 15 mL tube and slowly add 10 mL cell recovery solution while swirling the tube.
Centrifuge the cells at a relative centrifugal force of 200 x g for 5 minutes.
After centrifugation, aspirate supernatant and gently flick the tube to dislodge cells.
Resuspend cells by adding 2 mL cell recovery solution dropwise.
Perform a viable cell count and calculate the cell density of the cell solution.
Seed plate with the cell solution to achieve 30 to 70% confluence within 3 to 4 days. The differentiation efficiency of PSCs into cardiomyocytes varies between different PSC lines.
A critical variable for the generation of a robust cardiomyocyte culture is the relative confluence at the onset of differentiation. The suggested confluence range at the time of induction is 30 to 70%, and it is strongly recommended that you perform a confluence range finding study to determine the seeding density needed to achieve the optimal confluence for your PSC line within 3 to 4 days of seeding.
Confirm attachment of cells the following day.
Refeed cells with pre-warmed Essential 8 Medium every day for 3 to 4 days to reach target confluency for your PSC line.
Once the plate has achieved target confluency, replace the medium with Cardiomyocyte Differentiation Medium A. Note that the addition of medium should be done slowly and along the sides of the well, as forceful addition of medium will disrupt the differentiation process.
Two days after Cardiomyocyte Differentiation Medium A induction, replace the medium with Cardiomyocyte Differentiation Medium B.
Two days later, replace the medium with Cardiomyocyte Maintenance Medium.
Continue to refeed cells with this medium every other day.
10 days after differentiation has begun using the PSC Cardiomyocyte Differentiation Kit you should observe beating syncytia of cardiomyocytes. These cells are now ready for further studies.
#pscdifferentiation, #stemcelldifferentiation, #cardiomyocytedifferentiation, #differentiatingpluripotentstemcells, #PSCCardiomyocyteDifferentiationKit #thermofisherscientific
2013 Olympus BioScapes International Digital Imaging Competition
Specimen: Isolated living cardiomyocytes from murine (rodent) heart. The cells still have a "heartbeat."
Technique: Polarized light and differential interference contrast
Credit: Dr. Christopher B. Jackson
Prize: Honorable Mention
For more information, visit: Olympus-LifeScience.com
Find and Follow Olympus Life Science on:
Facebook: http://www.facebook.com/pages/Olympus-Life-Science/312676668924012/
Twitter: http://www.twitter.com/@OlympusLifeSci
LinkedIn: https://www.linkedin.com/company/olympus-life-science
Google+: https://plus.google.com/115358294493885697412/about#115358294493885697412/about
Instagram: http://instagram.com/olympuslifescience
See how muscle cells in the heart contract by allowing Calcium to flow inside and bringing along some positive charge with it! Rishi is a pediatric infectious disease physician and works at Khan Academy. Created by Rishi Desai.
Watch the next lesson: https://www.khanacademy.org/test-prep/nclex-rn/nclex-rn-circulatory-system/heart-depolarization-ddp/v/reseting-cardiac-concentration-gradients?utm_source=YT&utm_medium=Desc&utm_campaign=Nclex-rn
Missed the previous lesson? https://www.khanacademy.org/test-prep/nclex-rn/nclex-rn-circulatory-system/heart-depolarization-ddp/v/action-potentials-in-pacemaker-cells?utm_source=YT&utm_medium=Desc&utm_campaign=Nclex-rn
NCLEX-RN on Khan Academy: A collection of questions from content covered on the NCLEX-RN. These questions are available under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 United States License (available at http://creativecommons.org/licenses/by-nc-sa/3.0/us/).
About Khan Academy: Khan Academy offers practice exercises, instructional videos, and a personalized learning dashboard that empower learners to study at their own pace in and outside of the classroom. We tackle math, science, computer programming, history, art history, economics, and more. Our math missions guide learners from kindergarten to calculus using state-of-the-art, adaptive technology that identifies strengths and learning gaps. We've also partnered with institutions like NASA, The Museum of Modern Art, The California Academy of Sciences, and MIT to offer specialized content.
For free. For everyone. Forever. #YouCanLearnAnything
Subscribe to Khan Academy’s NCLEX-RN channel: https://www.youtube.com/channel/UCDx5cTeADCvKWgF9x_Qjz3g?sub_confirmation=1
Subscribe to Khan Academy: https://www.youtube.com/subscription_center?add_user=khanacademy
Our "how the heart works video" focuses on cardiomyocyte. Your heart beats approximately 100,000 times a day to circulate 2000 gallons of blood. The force exerted is similar to squeezing a tennis ball in your hand every second, 24 hours a day for your entire life. The heart has tremendous endurance.
Our "how the heart works video" focuses on cardiomyocyte. Your heart beats approximately 100,000 times a day to circulate 2000 gallons of blood. The force exerted is similar to squeezing a tennis ball in your hand every second, 24 hours a day for your entire life. The heart has tremendous endurance.
This video is about the cellular and molecular basis of cardiac pumping. Topics include the electrophysiological basis of the cardiac action potential (which triggers the heart beat), and how that action potential is coupled to calcium signaling, and how calcium triggers contraction.
https://www.thermofisher.com/us/en/home/references/protocols/cell-culture/stem-cell-protocols.html?cid=bid_clb_scl_r01_co_cp1361_pjt5757_bid88888_0vi_yut_vo_awa_kt_s24_SS519
In this video you will see how to use the Gibco PSC Cardiomyocyte Differentiation Kit to generate beating cardiomyocytes from pluripotent stem cells. By using this easy-to-use three-part media system, you can start from a small population of pluripotent stem cells and obtain a large number of beating cardiomyocytes. You can access the product insert and the quick reference protocol online.
A supplemental list of reagents used in this protocol can also be referenced here.
First, prepare a complete Essential 8 Medium. Find details for this preparation on the web link listed below.
Then prepare your substrate by coating a 12-well plate with a 1:100 Geltrex solution. And place in an incubator for at least one hour.
Next, prepare a cell recovery solution by adding 250 µm of RevitaCell supplement to 25 mL of Essential 8 Medium.
Let warm to room temperature.
Remove the Geltrex coated plate from the incubator and aspirate the plate.
Add 1 mL of cell recovery solution to each well.
Quickly thaw a vial of cryopreserved pluripotent stem cells in a 37˚C water bath until just a small ice crystal remains.
In the biosafety hood, transfer vial contents to a 15 mL tube and slowly add 10 mL cell recovery solution while swirling the tube.
Centrifuge the cells at a relative centrifugal force of 200 x g for 5 minutes.
After centrifugation, aspirate supernatant and gently flick the tube to dislodge cells.
Resuspend cells by adding 2 mL cell recovery solution dropwise.
Perform a viable cell count and calculate the cell density of the cell solution.
Seed plate with the cell solution to achieve 30 to 70% confluence within 3 to 4 days. The differentiation efficiency of PSCs into cardiomyocytes varies between different PSC lines.
A critical variable for the generation of a robust cardiomyocyte culture is the relative confluence at the onset of differentiation. The suggested confluence range at the time of induction is 30 to 70%, and it is strongly recommended that you perform a confluence range finding study to determine the seeding density needed to achieve the optimal confluence for your PSC line within 3 to 4 days of seeding.
Confirm attachment of cells the following day.
Refeed cells with pre-warmed Essential 8 Medium every day for 3 to 4 days to reach target confluency for your PSC line.
Once the plate has achieved target confluency, replace the medium with Cardiomyocyte Differentiation Medium A. Note that the addition of medium should be done slowly and along the sides of the well, as forceful addition of medium will disrupt the differentiation process.
Two days after Cardiomyocyte Differentiation Medium A induction, replace the medium with Cardiomyocyte Differentiation Medium B.
Two days later, replace the medium with Cardiomyocyte Maintenance Medium.
Continue to refeed cells with this medium every other day.
10 days after differentiation has begun using the PSC Cardiomyocyte Differentiation Kit you should observe beating syncytia of cardiomyocytes. These cells are now ready for further studies.
#pscdifferentiation, #stemcelldifferentiation, #cardiomyocytedifferentiation, #differentiatingpluripotentstemcells, #PSCCardiomyocyteDifferentiationKit #thermofisherscientific
2013 Olympus BioScapes International Digital Imaging Competition
Specimen: Isolated living cardiomyocytes from murine (rodent) heart. The cells still have a "heartbeat."
Technique: Polarized light and differential interference contrast
Credit: Dr. Christopher B. Jackson
Prize: Honorable Mention
For more information, visit: Olympus-LifeScience.com
Find and Follow Olympus Life Science on:
Facebook: http://www.facebook.com/pages/Olympus-Life-Science/312676668924012/
Twitter: http://www.twitter.com/@OlympusLifeSci
LinkedIn: https://www.linkedin.com/company/olympus-life-science
Google+: https://plus.google.com/115358294493885697412/about#115358294493885697412/about
Instagram: http://instagram.com/olympuslifescience
See how muscle cells in the heart contract by allowing Calcium to flow inside and bringing along some positive charge with it! Rishi is a pediatric infectious disease physician and works at Khan Academy. Created by Rishi Desai.
Watch the next lesson: https://www.khanacademy.org/test-prep/nclex-rn/nclex-rn-circulatory-system/heart-depolarization-ddp/v/reseting-cardiac-concentration-gradients?utm_source=YT&utm_medium=Desc&utm_campaign=Nclex-rn
Missed the previous lesson? https://www.khanacademy.org/test-prep/nclex-rn/nclex-rn-circulatory-system/heart-depolarization-ddp/v/action-potentials-in-pacemaker-cells?utm_source=YT&utm_medium=Desc&utm_campaign=Nclex-rn
NCLEX-RN on Khan Academy: A collection of questions from content covered on the NCLEX-RN. These questions are available under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 United States License (available at http://creativecommons.org/licenses/by-nc-sa/3.0/us/).
About Khan Academy: Khan Academy offers practice exercises, instructional videos, and a personalized learning dashboard that empower learners to study at their own pace in and outside of the classroom. We tackle math, science, computer programming, history, art history, economics, and more. Our math missions guide learners from kindergarten to calculus using state-of-the-art, adaptive technology that identifies strengths and learning gaps. We've also partnered with institutions like NASA, The Museum of Modern Art, The California Academy of Sciences, and MIT to offer specialized content.
For free. For everyone. Forever. #YouCanLearnAnything
Subscribe to Khan Academy’s NCLEX-RN channel: https://www.youtube.com/channel/UCDx5cTeADCvKWgF9x_Qjz3g?sub_confirmation=1
Subscribe to Khan Academy: https://www.youtube.com/subscription_center?add_user=khanacademy
Cardiac muscle cells or cardiomyocytes (also known as myocardiocytes or cardiac myocytes) are the muscle cells (myocytes) that make up the cardiac muscle. Each myocardial cell contains myofibrils, which are specialized organelles consisting of long chains of sarcomeres, the fundamental contractile units of muscle cells. Cardiomyocytes show striations similar to those on skeletal muscle cells. Unlike multinucleated skeletal cells, the majority of cardiomyocytes contain only one nucleus, although they may have as many as four. Cardiomyocytes have a high mitochondrial density, which allows them to produce adenosine triphosphate (ATP) quickly, making them highly resistant to fatigue.
Types of cells
There are two types of cells within the heart: the cardiomyocytes and the cardiac pacemaker cells.
Cardiomyocytes make up the atria (the chambers in which blood enters the heart) and the ventricles (the chambers where blood is collected and pumped out of the heart). These cells must be able to shorten and lengthen their fibers and the fibers must be flexible enough to stretch. These functions are critical to the proper form during the beating of the heart.
hiPSC-derived cardiomyocytes are promising resources for drug discovery and regenerative medicine ... Specifically, the ventricle and atrium consist of ventricular cardiomyocytes (VCMs) and atrial cardiomyocytes (ACMs), respectively.
Heartseed has successfully produced high-purity cardiomyocytes consistently from all I Peace manufactured iPS cell lines used in this study ... Significance of stable production of high-purity cardiomyocytes derived from iPS cells.
Heartseed is developing HS-001 (open chest administration) and HS-005 (catheter administration), which are microtissues of highly purified cardiomyocytes (cardiomyocyte spheroids) generated from ...
Heartseed is developing HS-001 (open chest administration) and HS-005 (catheter administration), which are microtissues of highly purified cardiomyocytes (cardiomyocyte spheroids) generated from ...
By combining calcium and electrical pacing, researchers designed a scalable protocol for culturing mature cardiac tissues from induced pluripotent stem cells ... .
(MENAFN - PR Newswire) Developed in collaboration with REPROCELL, CELLvoTM Atrial Cardiomyocyte delivers non-genetically modified human heart cells that are chamber specific and derived from iPS ... .
Developed in collaboration with REPROCELL, CELLvo™ Atrial Cardiomyocyte delivers non-genetically modified human heart cells that are chamber specific and derived from iPS (stem) cells ... cardiomyocytes.
The mechanism of action is that the transplanted cardiomyocytes electrically couple with the patient's myocardium to generate contractile force (remuscularization) ... "iPSC-cardiomyocytes might represent ...