The sample preparation for mass spectrometry is used for the optimisation of a sample for the analysis in a mass spectrometer (MS).
These have depending on their ion source different requirements for volume, concentration, and composition of the analyte solution. Furthermore, in protein mass spectrometry usually the protein of interest is cleaved into peptides before analysis, either by in-gel digestion or by proteolysis in solution.
The concentration of salt in the sample is thereby not so critical as it is for electrospray ionization. However, interfering signals are observed due to side reactions of the matrix with alkali metal ions which can impair the analysis of the spectra. Nevertheless, a separate desalting step is usually not necessary as in previous steps of sample preparation e.g. in-gel digestion of proteins, the selection of appropriate buffer salts prevents the occurrence of this problem. Furthermore the crystallised matrix/analyte mixture allows removing of salts by washing on the target and the addition of ammonium phosphate before crystallisation can improve the signal quality.
In analytical chemistry, sample preparation refers to the ways in which a sample is treated prior to its analysis. Preparation is a very important step in most analytical techniques, because the techniques are often not responsive to the analyte in its in-situ form, or the results are distorted by interfering species. Sample preparation may involve dissolution, reaction with some chemical species, pulverizing, treatment with a chelating agent (e.g. EDTA), masking, filtering, dilution, sub-sampling or many other techniques.
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Latest News for: 2) next generation sequencing (ngs) sample preparation
... leading global provider of Sample to Insight solutions, announced they will leverage their technologies to increase the availability of sample preparation kits for next-generation sequencing (NGS).
... from sample preparation to data analysis. These systems employ advanced sequencing technologies, such as next-generation sequencing (NGS), to enable high-throughput sequencing and genetic analysis.
... next generation of life sciences innovation, especially through its Next-Generation Sequencing (NGS) capability, for efficient, reproducible and high-throughput sample preparation and data analysis.
QIAGEN will provide Sample to Insight solutions, including sample preparation, its PCR, digital PCR (using the QIAcuity system), QIAseq next-generation sequencing (NGS) technologies, instruments, as ...
In 2022, consumables dominate the product segment of the global next generation sequencing (NGS) market owing to the supplies such as reagents, kits, and consumables are needed for sample preparation, ...
(MENAFN - GlobeNewsWire - Nasdaq) New York, May 17, 2023 (GLOBE NEWSWIRE) -- Reportlinker.com announces the release of the report "GlobalNextGeneration Sequencing (NGS) Sample Preparation ... .
... such as next-generation sequencing (NGS) and others and the advancement in nucleic acid extraction/isolation techniques have driven the growth of the nucleic acid sample preparation market.
... such as next-generation sequencing (NGS) and others and the advancement in nucleic acid extraction/isolation techniques have driven the growth of the nucleic acid sample preparation market.
...DNA Technologies' newly launched xGen Monkeypox Virus Amplicon Panel is a complete next generation sequencing (NGS) library preparation solution that supports the genotyping of MPXV within a sample.
... world, today announced the launch of the research use only (RUO) BioQule™ NGSSystem – an automated benchtop solution for next-generation sequencing (NGS) library preparation of up to eight samples.
S2 Genomics’ technology platforms integrate advanced fluidics, optics, and biochemistry to produce automated sample preparation solutions for the next generation sequencing (NGS) and cell biology ...
Victoria Advocate
08 Feb 2024
Victoria Advocate
08 Feb 2024