Gelan tetrasaharidna nezasićeno glukuronilna hidrolaza
| Gelan tetrasaharidna nezasićeno glukuronilna hidrolaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 3.2.1.179 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
Gelan tetrasaharidna nezasićeno glukuronilna hidrolaza (EC 3.2.1.179, UGL, hidrolaza nezasićenog glukuronila) je enzim sa sistematskim imenom beta-D-4-dezoksi-Delta4-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alfa-L-Rhap-(1->3)-beta-D-Glcp beta-D-4-dezoksi-Delta4-GlcAp hidrolaza.[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju
- beta-D-4-dezoksi-Delta4-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alfa-L-Rhap-(1->3)-beta-D-Glcp + H 2O
5-dehidro-4-dezoksi-D-glukuronat + beta-D-Glcp-(1->4)-alfa-L-Rhap-(1->3)-beta-D-Glcp
Ovaj enzim odvaja 4-dezoksi-4(5)-nezasićene D-glucuronske kiselina sa oligosaharida koje formiraju polisaharidne lijaze.
- ↑ Itoh, T., Akao, S., Hashimoto, W., Mikami, B. and Murata, K. (2004). „Crystal structure of unsaturated glucuronyl hydrolase, responsible for the degradation of glycosaminoglycan, from Bacillus sp. GL1 at 1.8 Å resolution”. J. Biol. Chem. 279: 31804-31812. PMID 15148314.
- ↑ Hashimoto, W., Kobayashi, E., Nankai, H., Sato, N., Miya, T., Kawai, S. and Murata, K. (1999). „Unsaturated glucuronyl hydrolase of Bacillus sp. GL1: novel enzyme prerequisite for metabolism of unsaturated oligosaccharides produced by polysaccharide lyases”. Arch. Biochem. Biophys. 368: 367-374. PMID 10441389.
- ↑ Itoh, T., Hashimoto, W., Mikami, B. and Murata, K. (2006). „Substrate recognition by unsaturated glucuronyl hydrolase from Bacillus sp. GL1”. Biochem. Biophys. Res. Commun. 344: 253-262. PMID 16630576.
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