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. 2010 May 26:9:53.
doi: 10.1186/1476-511X-9-53.

Soybean oil increases SERCA2a expression and left ventricular contractility in rats without change in arterial blood pressure

Rogério Faustino Ribeiro Junior  1 , Aurélia Araújo Fernandes, Eduardo Frizzera Meira, Priscila Rossi Batista, Fabiana Dayse Magalhães Siman, Dalton Valentim Vassallo, Alessandra Simão Padilha, Ivanita Stefanon
Affiliations

Soybean oil increases SERCA2a expression and left ventricular contractility in rats without change in arterial blood pressure

Rogério Faustino Ribeiro Junior et al. Lipids Health Dis. .

Abstract

Background: Our aim was to evaluate the effects of soybean oil treatment for 15 days on arterial and ventricular pressure, myocardial mechanics and proteins involved in calcium handling.

Methods: Wistar rats were divided in two groups receiving 100 microL of soybean oil (SB) or saline (CT) i.m. for 15 days. Ventricular performance was analyzed in male 12-weeks old Wistar rats by measuring left ventricle diastolic and systolic pressure in isolated perfused hearts according to the Langendorff technique. Protein expression was measured by Western blot analysis.

Results: Systolic and diastolic arterial pressures did not differ between CT and SB rats. However, heart rate was reduced in the SB group. In the perfused hearts, left ventricular isovolumetric systolic pressure was higher in the SB hearts. The inotropic response to extracellular Ca2+ and isoproterenol was higher in the soybean-treated animals than in the control group. Myosin ATPase and Na(+)-K(+)ATPase activities, the expression of sarcoplasmic reticulum calcium pump (SERCA2a) and sodium calcium exchanger (NCX) were increased in the SB group. Although the phosfolamban (PLB) expression did not change, its phosphorylation at Ser16 was reduced while the SERCA2a/PLB ratio was increased.

Conclusions: In summary, soybean treatment for 15 days in rats increases the left ventricular performance without affecting arterial blood pressure. These changes might be associated with an increase in the myosin ATPase activity and SERCA2a expression.

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Figures

Figure 1
Figure 1
(A) LVISP-Left ventricle isovolumetric systolic pressure curves obtained at different diastolic pressures (DP). (B) LVISP upon changes in heart rate stimulation. (C) Left ventricular curves were normalized to the DP of 10 mm Hg. Hearts were perfused using different extracellular Ca2+ concentrations. (D) β-adrenergic activation in the Langendorff apparatus (% of Control). Data are shown as mean ± SEM. *p < 0.05. ANOVA two-way and Tukey test. Number of animals used is indicated in parentheses.
Figure 2
Figure 2
Myosin ATPase activity (A) and Na+-K+ ATPase activity (B). Data represent mean ± SEM. *p < 0.05. Student "t" test. Number of animals used is indicated in parentheses.
Figure 3
Figure 3
Densitometric analysis of the Western blot for SERCA2a, sodium-calcium exchange (NCX), phospholamban (PLB), phosphorylated phospholamban (PLB-phospho-Ser16), SERCA/PLB ratio, isoforms α1 of Na+-K+ ATPase, and α2 of Na+-K+ ATPase protein expression in the hearts from control and SB-treated rats. *p < 0.05 by Student's t-test. Number of animals used is indicated in parentheses. Representative blots are shown.
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