Hi @biofuture

• I installed the singularity 3.8 version on ubuntu now I dont understand how to install args-oap application, I would appreciate if you could guide me in doing that. I have already downloaded the sif file but how does it get installed?

• it is mentioned that the fastq files should be in .fq format but I am afraid since my files are whole shotgun metagenome datasets so they are pretty large in size and are in compressed format i.e .fastq.gz format. So, if I convert them to .fq I probably have to decompress the files or should I simply change the file names to read_R1.fq?

• I have another doubt that I already have sample Ids like my samples are named as A1_R1.fastq.gz, A2_R2.fastq.gz, A2_R1.fastq.gz, A2_R2.fastq.gz ..A3_R1,A3_R2.....A90_R1.fastq.gz, A90_R2.fastq.gz. So what should be the sample Ids and sample names? I hope I get a response to these questions because without the answers to these I would not be able to start my analysis.

Thanks in Advance
Saraswati Awasthi