SECTM1
Izlučeni i transmembranski protein 1 je protein koji je kod ljudi kodiran genom SECTM1.[5][6]
Aminokiselinska sekvenca
- Simboli
C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin
| 10 | 20 | 30 | 40 | 50 | ||||
|---|---|---|---|---|---|---|---|---|
| MQTCPLAFPG | HVSQALGTLL | FLAASLSAQN | EGWDSPICTE | GVVSVSWGEN | ||||
| TVMSCNISNA | FSHVNIKLRA | HGQESAIFNE | VAPGYFSRDG | WQLQVQGGVA | ||||
| QLVIKGARDS | HAGLYMWHLV | GHQRNNRQVT | LEVSGAEPQS | APDTGFWPVP | ||||
| AVVTAVFILL | VALVMFAWYR | CRCSQQRREK | KFFLLEPQMK | VAALRAGAQQ | ||||
| GLSRASAELW | TPDSEPTPRP | LALVFKPSPL | GALELLSPQP | LFPYAADP |
Kloniranje i eksresija
[uredi | uredi izvor]Ovaj gen kodira transmembranski i izlučeni protein sa karakteristikamatransmembranskih proreina tipa 1a. Nalazi se u perijedarnom Golgijevom obrascu i smatra se da je uključen u procese hematopoeze i/ili imunskog sistema.[6]
Dužina polipeptidnog lanca je 248 aminokiselina, a molekulska težina 27.039[7].
Analizom DNK-hipersenzibilne regije, otprilike 5 kb uzvodno od gena CD7 na 17q25.2-q25.3, Slentz-Kesler et ak. (1998) klonirali su SECTM1 cDNK, koju su nazvali K12. Northern blot analiza otkrila je da se K12 eksprimira kao iRNK od 1,8 kb u mnogim testiranim tkivima, sa najvišim nivoom ekspresije u leukocitima periferne krvi. Predviđeni protein K12 od 248 aminokiselina ima hidropatske karakteristike transmembranskog proteina tipa 1a, koji je definiran vanćelijskim N-krajem i odcijepljenim signalnim peptidom. Autori su pokazali da se protein K12 biohemijski ponaša kao integralni membranski protein. Koristeći imunofluorescenciju, lokalizirali su K12 na Golgijevom aparatu. Western blotiranje pokazalo je da protein K12 migrira kao klaster traka na 27 kD. Pored toga, ćelije luče približno 20 kD rastvorljivog oblika K12. Utvrdili su da gen K12 sadrži četiri egzona i da se proteže približno na 14 kb.[8]
Reference
[uredi | uredi izvor]- ^ a b c GRCh38: Ensembl release 89: ENSG00000141574 - Ensembl, maj 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000025165 - Ensembl, maj 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ Slentz-Kesler KA, Hale LP, Kaufman RE (Apr 1998). "Identification and characterization of K12 (SECTM1), a novel human gene that encodes a Golgi-associated protein with transmembrane and secreted isoforms". Genomics. 47 (3): 327–40. doi:10.1006/geno.1997.5151. PMID 9480746.
- ^ a b "Entrez Gene: SECTM1 secreted and transmembrane 1".
- ^ "UniProt, Q8WVN". Pristupljeno 11. 6. 2021.
- ^ Slentz-Kesler, K. A., Hale, L. P., Kaufman, R. E. Identification and characterization of K12 (SECTM1), a novel human gene that encodes a golgi-associated protein with transmembrane and secreted isoforms. Genomics 47: 327-340, 1998. [PubMed]: 9480746
Dopunska literatura
[uredi | uredi izvor]- Kimura K, Wakamatsu A, Suzuki Y, et al. (2006). "Diversification of transcriptional modulation: Large-scale identification and characterization of putative alternative promoters of human genes". Genome Res. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC 1356129. PMID 16344560.
- Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
- Matsuda A, Suzuki Y, Honda G, et al. (2003). "Large-scale identification and characterization of human genes that activate NF-kappaB and MAPK signaling pathways". Oncogene. 22 (21): 3307–18. doi:10.1038/sj.onc.1206406. PMID 12761501.
- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
- Lyman SD, Escobar S, Rousseau AM, et al. (2000). "Identification of CD7 as a cognate of the human K12 (SECTM1) protein". J. Biol. Chem. 275 (5): 3431–7. doi:10.1074/jbc.275.5.3431. PMID 10652336.
- Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
- Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.