PLXDC1

PLXDC1
Identifikatori
Aliasi	PLXDC1
Vanjski ID-jevi	OMIM: 606826 MGI: 1919574 HomoloGene: 10700 GeneCards: PLXDC1
Lokacija gena (čovjek)
Hrom.	Hromosom 17 (čovjek)
Kraj	39,154,394 bp
Lokacija gena (miš)
Hrom.	Hromosom 11 (miš)
Kraj	97,877,270 bp
Obrazac RNK ekspresije
	; ;
	Više referentnih podataka o ekspresiji
Ontologija gena
Molekularna funkcija	• GO:0001948, GO:0016582 vezivanje za proteine;
Ćelijska komponenta	• citoplazma; • integral component of membrane; • extracellular region; • međućelijske veze; • soma; • receptor complex; • ćelijska membrana; • dendrit; • intracellular anatomical structure; • membrana; • bicellular tight junction; • Vanćelijsko;
Biološki proces	• Angiogeneza; • spinal cord development;
	Izvori:Amigo / QuickGO
Ortolozi
	57125
	72324
	ENSG00000161381
	ENSMUSG00000017417
	Q8IUK5
	Q91ZV7
	NM_020405
	NM_001163608; NM_028199
	NP_065138
	NP_001157080; NP_082475
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

Pleksinski protein 1 sa domenom je protein koji je kod ljudi kodiran genom PLXDC1.^[5]^[6]^[7]

Dužina polipeptidnog lanca je 500 aminokiselina, sa molekulskom težinom od 55.760.^[8]

Aminokiselinska sekvenca

Simboli

C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin

10	20	30	40	50
MRGELWLLVL	VLREAARALS	PQPGAGHDEG	PGSGWAAKGT	VRGWNRRARE
SPGHVSEPDR	TQLSQDLGGG	TLAMDTLPDN	RTRVVEDNHS	YYVSRLYGPS
EPHSRELWVD	VAEANRSQVK	IHTILSNTHR	QASRVVLSFD	FPFYGHPLRQ
ITIATGGFIF	MGDVIHRMLT	ATQYVAPLMA	NFNPGYSDNS	TVVYFDNGTV
FVVQWDHVYL	QGWEDKGSFT	FQAALHHDGR	IVFAYKEIPM	SVPEISSSQH
PVKTGLSDAF	MILNPSPDVP	ESRRRSIFEY	HRIELDPSKV	TSMSAVEFTP
LPTCLQHRSC	DACMSSDLTF	NCSWCHVLQR	CSSGFDRYRQ	EWMDYGCAQE
AEGRMCEDFQ	DEDHDSASPD	TSFSPYDGDL	TTTSSSLFID	SLTTEDDTKL
NPYAGGDGLQ	NNLSPKTKGT	PVHLGTIVGI	VLAVLLVAAI	ILAGIYINGH
PTSNAALFFI	ERRPHHWPAM	KFRSHPDHST	YAEVEPSGHE	KEGFMEAEQC

Kloniranje i ekspresija

Koristeći serijsku analizu ekspresija gena (SAGE), St Croix et al. (2000) identificirali su parcijalne cDNK, koje odgovaraju za nekoliko tumorskih endotelnih markera (TEM) koji su pokazali povišenu ekspresiju tokom tumorske angiogeneze. Među genima koje su identificirali bio je PLXDC1, koji su nazvali TEM7. Korištenjem pretraživanja baze podataka i RACE-a s pet najboljih, Carson-Walter et al. (2001) izveli su sekvence cijele regiju kodiranja TEM7, koja kodira 500-aminokiselinski tip I transmembranskog proteina koji sadrži domen sličan pleksinu. Zamjenski transkript gena TEM7 označili su TEM3. TEM3 i TEM7 se razlikuju u upotrebi alternativnih mjesta poliadenilacije, ali rezultiraju istim predviđenim proteinima. Mišji ortolog TEM7 dijeli 81% aminokiselinskih identiteta s ljudskim proteinima.

Analiza hibridizacije na ljudskog karcinoma debelog crijeva od Carson-Walter et al. (2001) pokazali su da se TEM7 jasno eksprimira u endotelnim ćelijama tumorske strome, ali ne i u endotelnim ćelijama normalnog tkiva debelog crijeva. Koristeći fluorescentnu hibridizaciju in situ za ispitivanje ekspresije u raznim normalnim tkivima odraslog miša, primijetili su da Tem7 uglavnom nije moguće otkriti u tkivima ili tumorima, ali je obilno eksprimiran u mišjem mozgu. Lokalizirali su ekspresiju Tem7 u Purkinjeovim ćelijama malog mozga i nekim neuronskim ćelijama.^[9]^[10]

Reference

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000161381 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000017417 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ St Croix B, Rago C, Velculescu V, Traverso G, Romans KE, Montgomery E, Lal A, Riggins GJ, Lengauer C, Vogelstein B, Kinzler KW (Aug 2000). "Genes expressed in human tumor endothelium". Science. 289 (5482): 1197–202. doi:10.1126/science.289.5482.1197. PMID 10947988.
^ Carson-Walter EB, Watkins DN, Nanda A, Vogelstein B, Kinzler KW, St Croix B (Sep 2001). "Cell surface tumor endothelial markers are conserved in mice and humans". Cancer Res. 61 (18): 6649–55. PMID 11559528.
^ "Entrez Gene: PLXDC1 plexin domain containing 1".
^ "UniProt, Q8IUK5". Pristupljeno September 12, 2017.
^ Carson-Walter, E. B., Watkins, D. N., Nanda, A., Vogelstein, B., Kinzler, K. W., St. Croix, B. Cell surface tumor endothelial markers are conserved in mice and humans. Cancer Res. 61: 6649-6655, 2001. PubMed: 11559528
^ St. Croix, B., Rago, C., Velculescu, V., Traverso, G., Romans, K. E., Montgomery, E., Lal, A., Riggins, G. J., Lengauer, C., Vogelstein, B., Kinzler, K. W. Genes expressed in human tumor endothelium. Science 289: 1197-1202, 2000. [PubMed]: 10947988

Dopunska literatura

Fuchs B, Mahlum E, Halder C, et al. (2007). "High expression of tumor endothelial marker 7 is associated with metastasis and poor survival of patients with osteogenic sarcoma". Gene. 399 (2): 137–43. doi:10.1016/j.gene.2007.05.003. PMC 2066185. PMID 17560052.
Nanda A, Buckhaults P, Seaman S, et al. (2005). "Identification of a binding partner for the endothelial cell surface proteins TEM7 and TEM7R". Cancer Res. 64 (23): 8507–11. doi:10.1158/0008-5472.CAN-04-2716. PMID 15574754.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Yu W, Andersson B, Worley KC, et al. (1997). "Large-scale concatenation cDNA sequencing". Genome Res. 7 (4): 353–8. doi:10.1101/gr.7.4.353. PMC 139146. PMID 9110174.
Andersson B, Wentland MA, Ricafrente JY, et al. (1996). "A "double adaptor" method for improved shotgun library construction". Anal. Biochem. 236 (1): 107–13. doi:10.1006/abio.1996.0138. PMID 8619474.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000161381 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000017417 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid10947988-5] St Croix B, Rago C, Velculescu V, Traverso G, Romans KE, Montgomery E, Lal A, Riggins GJ, Lengauer C, Vogelstein B, Kinzler KW (Aug 2000). "Genes expressed in human tumor endothelium". Science. 289 (5482): 1197–202. doi:10.1126/science.289.5482.1197. PMID 10947988.

[pmid11559528-6] Carson-Walter EB, Watkins DN, Nanda A, Vogelstein B, Kinzler KW, St Croix B (Sep 2001). "Cell surface tumor endothelial markers are conserved in mice and humans". Cancer Res. 61 (18): 6649–55. PMID 11559528.

[entrez-7] "Entrez Gene: PLXDC1 plexin domain containing 1".

[UniProt-8] "UniProt, Q8IUK5". Pristupljeno September 12, 2017.

[9] Carson-Walter, E. B., Watkins, D. N., Nanda, A., Vogelstein, B., Kinzler, K. W., St. Croix, B. Cell surface tumor endothelial markers are conserved in mice and humans. Cancer Res. 61: 6649-6655, 2001. PubMed: 11559528

[10] St. Croix, B., Rago, C., Velculescu, V., Traverso, G., Romans, K. E., Montgomery, E., Lal, A., Riggins, G. J., Lengauer, C., Vogelstein, B., Kinzler, K. W. Genes expressed in human tumor endothelium. Science 289: 1197-1202, 2000. [PubMed]: 10947988

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