NRSN2

NRSN2
Identifikatori
Aliasi	NRSN2
Vanjski ID-jevi	OMIM: 610666 MGI: 2684969 HomoloGene: 11802 GeneCards: NRSN2
Lokacija gena (čovjek)
Hrom.	Hromosom 20 (čovjek)
Kraj	359,660 bp
Lokacija gena (miš)
Hrom.	Hromosom 2 (miš)
Kraj	152,218,558 bp
Obrazac RNK ekspresije
	Više referentnih podataka o ekspresiji
Ontologija gena
Molekularna funkcija	• molekularna funkcija;
Ćelijska komponenta	• integral component of membrane; • soma; • ćelijska membrana; • transport vesicle; • membrana; • GO:0016023 citoplazmatska vezikula; • neuron projection;
Biološki proces	• GO:0022610 biološki proces; • nervous system development;
	Izvori:Amigo / QuickGO
Ortolozi
	80023
	228777
	ENSG00000125841
	ENSMUSG00000059361
	Q9GZP1
	Q5HZK2
NM_024958; NM_001323679; NM_001323680; NM_001323681; NM_001323682;
	NM_001323683; NM_001323684; NM_001323685
	NM_001009948; NM_001370749; NM_001370750
NP_001310608; NP_001310609; NP_001310610; NP_001310611; NP_001310612;
	NP_001310613; NP_001310614; NP_079234
	NP_001009948; NP_001357678; NP_001357679
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

Neurenzin-2 je protein koji je kod ljudi kodiran genom NRSN2.^[5]^[6]

Kloniranje i ekspresija

Koristeći BLAST analizu sa sekvencom mišjeg neurezina-1 (NRSN1; 616630) kao upit, Nakanishi et al. (2006) su identifikovali sekvence mišjeg i ljudskog homolognog gena koje su nazvali neurenzin-2 (NRSN2). Ljudski protein NRSN2 sadrži dva domena koja se protežu kroz membranu i dijeli 41% identiteta s mišjimm Nrsn1. Northern blot analiza mišjih tkiva pokazala je specifičnu ekspresiju za mozak 1,7-kb Nrsn2 transkripta. Imunoblot analiza pokazala je ekspresiju proteina Nrsn2 15,5 embrionskog dana, sa snažnom ekspresijom viđenom od 7 -14 postnatusnih dana i u dobi od 10 sedmica. Imunoblot analiza moždanog područja pokazala je da je Nrsn2 široko eksprimiran kroz mozak, sa najvećom ekspresijom u talamusu/hipotalamusu. Indirektna imunofluorescencija u transficiranim Neuro2a ćelijama pokazala je da se Nrsn2 lokalizira u perijedarne organele , sa procesima u regijama različitim od onih obojenih Nrsn1, a u transformiranim COS-7 ćelijama pokazalo je da se Nrsn2 lokalizira u lizosomima. Imunohistohemijska analiza u mozgu miša pokazala je sličnu ukupnu raspodjelu Nrsn2 i Nrsn1, ali različite subćelijske lokalizacije, s Nrsn2 u somatskim regijama i Nrsn1 u neuritskim procesima. Nrsn2-pozitivne ćelije su viđene septumskom jezgru, dijagonalnom pojasu, hipokampusu, amigdaloidnom jezgru, jezgru habenula i pokrovi moždane kore, a istaknuto imunobojanje viđeno je u ćelijskim tijelima cerebelarnih Purkinjeovih ćelija.^[7]

Molekulska funkcija

integralna komponenta membrane
tijelo nervnih ćelija
plazmamembrana
transportna vezikula
membrana
GO:0016023 citoplazmatska vezikula
projekcije neurona

Biološki procesi

biološki promet
razvoj nervnog sistema

Izvori:Amigo/QuickGO

Aminokiselinska sekvenca

10	20	30	40	50
MMPSCNRSCS	CSRGPSVEDG	KWYGVRSYLH</ td>	LFYEDCAGTA	LSDDPEGPPV
LCPRRPWPSL	CWKISLSSGT	LLLLLGVAAL	TTGYAVPPKL	EGIGEGEFLV
LDQRAADYNQ	ALGTCRLAGT	ALCVAAGVLL	AICLFWAMIG	WLSQDTKAEP
LDPEADSHVE	VFGDEPEQQL	SPIFRNASGQ	SWFSPPASPF	GQSSVQTIQP
KRDS

Oznake aminokiselina

C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin

Reference

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000125841 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000059361 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Nakanishi K, Ida M, Suzuki H, Kitano C, Yamamoto A, Mori N, Araki M, Taketani S (Apr 2006). "Molecular characterization of a transport vesicle protein Neurensin-2, a homologue of Neurensin-1, expressed in neural cells". Brain Res. 1081 (1): 1–8. doi:10.1016/j.brainres.2006.01.085. PMID 16527258.
^ "Entrez Gene: NRSN2 neurensin 2".
^ Nakanishi, K., Ida, M., Suzuki, H., Kitano, C., Yamamoto, A., Mori, N., Araki, M., Taketani, S. Molecular characterization of a transport vesicle protein neurensin-2, a homologue of neurensin-1, expressed in neural cells. Brain Res. 1081: 1-8, 2006. PubMed: 16527258.

Dopunska literarura

Hartley JL, Temple GF, Brasch MA (2001). "DNA Cloning Using In Vitro Site-Specific Recombination". Genome Res. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863.
Wiemann S, Weil B, Wellenreuther R, et al. (2001). "Toward a Catalog of Human Genes and Proteins: Sequencing and Analysis of 500 Novel Complete Protein Coding Human cDNAs". Genome Res. 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166.
Simpson JC, Wellenreuther R, Poustka A, et al. (2001). "Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing". EMBO Rep. 1 (3): 287–92. doi:10.1093/embo-reports/kvd058. PMC 1083732. PMID 11256614.
Deloukas P, Matthews LH, Ashurst J, et al. (2002). "The DNA sequence and comparative analysis of human chromosome 20". Nature. 414 (6866): 865–71. doi:10.1038/414865a. PMID 11780052.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
Wiemann S, Arlt D, Huber W, et al. (2004). "From ORFeome to Biology: A Functional Genomics Pipeline". Genome Res. 14 (10B): 2136–44. doi:10.1101/gr.2576704. PMC 528930. PMID 15489336.
Mehrle A; Rosenfelder H; Schupp I; et al. (2006). "The LIFEdb database in 2006". Nucleic Acids Res. 34 (Database issue): D415–8. doi:10.1093/nar/gkj139. PMC 1347501. PMID 16381901.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000125841 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000059361 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid16527258-5] Nakanishi K, Ida M, Suzuki H, Kitano C, Yamamoto A, Mori N, Araki M, Taketani S (Apr 2006). "Molecular characterization of a transport vesicle protein Neurensin-2, a homologue of Neurensin-1, expressed in neural cells". Brain Res. 1081 (1): 1–8. doi:10.1016/j.brainres.2006.01.085. PMID 16527258.

[entrez-6] "Entrez Gene: NRSN2 neurensin 2".

[7] Nakanishi, K., Ida, M., Suzuki, H., Kitano, C., Yamamoto, A., Mori, N., Araki, M., Taketani, S. Molecular characterization of a transport vesicle protein neurensin-2, a homologue of neurensin-1, expressed in neural cells. Brain Res. 1081: 1-8, 2006. PubMed: 16527258.

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