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LC-MS/MS derived peptide retention time deviation calculator across replicates for DDA and DIA derived result files.

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UniformRT

LC-MS/MS derived peptide retention time deviation calculator across replicates for DDA and DIA derived result files.

How to use UniformRT in Windows/Linux

usage: UniformRT.py [-h] -ex [-ex ...] -ip [-ip ...] -rt [-rt ...]

Variation from run-to-run retention time (RT) deviation in LC-MS derived DDA
or DIA results

positional arguments:
  -ex         Mention the type of acquisition method used (DDA/DIA)
  -ip         This tool can take Peptide Spectrum Match (PSM) table from
              Proteome Discoverer or DIA-Spectral Library search output from
              Skyline in tab delimitted format (txt/tsv)
  -rt         Minimum number of RTs to be considered for the analysis

optional arguments:
  -h, --help  show this help message and exit

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LC-MS/MS derived peptide retention time deviation calculator across replicates for DDA and DIA derived result files.

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