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B_ALL_genome_wide_crispr

Count Data

Broad PoolQ count data for the primary screen are located in these directories:

  • 200406
  • 200627
  • RH_resequenced_counts_files

Broad PoolQ count data for the validation screen are located in these directories:

  • crispr_041822_validationScreen/fromBroad

Primary screen

data were imported and organized using the script:

  • mageck/mageckPrimary_Screen.Rmd

Pool/Sample assembled count matricies are in mageck directory:

The files are:

  • invitro.pool[ABCDEF].txt
  • invivo.pool[ABCDEF].txt

mageck (version 0.5.9.4) test was run on Linux system with these scripts:

  • mageck/mageck_invitro_pool[ABCDEF].sh
  • mageck/mageck_invivo_pool[ABCDEF].sh

all associated mageck output for these runs are included in the mageck folder

Validation screen

Broad PoolQ count data for the validation screen are located in this directory:

  • crispr_041822_validationScreen/fromBroad

data were imported and organized using the script:

  • crispr_041822_validationScreen/mageck/mageckFlute_RamosValidationScreen.html

mageck (version 0.5.9.4) test was run on Linux system with this script:

  • crispr_041822_validationScreen/mageck/mageck_sr.sh

mageck output for this run is included in the mageck folder

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sgRNA count data for Ramos, Koch et al

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