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Update Readme.md
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srinew authored Jan 16, 2023
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Link to app: https://svnallan.shinyapps.io/heatmap_shiny/

# Sample Annotations:
First row is for Sample IDs which will be header, second row for sample annotations e.g Tumor, Normal which will be taken as factors to annotate the heatmap.
First row is Sample IDs for header, second row for sample annotations e.g Tumor, Normal which will annotate the heatmap.
Gene annotations (first column) are always followed by Control samples first e.g

Column1: Gene symbols
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Column5: Tumor2

# Gene Annotations:
First column is for gene symbols/identifiers, cannot be duplicates. Housekeeping genes can be selected manually e.g ACTB, GAPDH, TUBB.
First column is gene symbols/identifiers, cannot be duplicates. Housekeeping genes can be selected manually e.g ACTB, GAPDH, TUBB.

# Normalization:
log2(2^(-ddCT)+1)

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