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Rose Waaler Test: Jayan Bajracharya - B.SC - MLT 3 Year

The Rose Waaler test is a haemagglutination slide test used to detect rheumatoid factor (RF) in patient sera. It uses sheep red blood cells sensitized with rabbit antibodies, which causes agglutination if RF is present. The test is simple, fast (results in 2 minutes), and cost-effective. A positive result appears as red agglutinates against a blue background, indicating the presence of RF, while a negative result shows no agglutination. The test can also be performed semi-quantitatively by making serial dilutions of the serum sample.

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Rajkishor Yadav
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3K views10 pages

Rose Waaler Test: Jayan Bajracharya - B.SC - MLT 3 Year

The Rose Waaler test is a haemagglutination slide test used to detect rheumatoid factor (RF) in patient sera. It uses sheep red blood cells sensitized with rabbit antibodies, which causes agglutination if RF is present. The test is simple, fast (results in 2 minutes), and cost-effective. A positive result appears as red agglutinates against a blue background, indicating the presence of RF, while a negative result shows no agglutination. The test can also be performed semi-quantitatively by making serial dilutions of the serum sample.

Uploaded by

Rajkishor Yadav
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd

Rose Waaler Test

-JAYAN BAJRACHARYA
-[Link] 3rd year
INTRODUCTION
 ROSE WAALER test is a haemagglutination slide test for
the rapid qualitative and semi-quantitative détection of IgM
Rheumatoid Factor in sera from most patients with
rheumatoid arthritis.

It is a special type of passive haemagglutination test . In


rheumatoid arthritis , an autoantibody (RA factor) appears in
the serum, which acts an antibody to gamma globulin.

The antigen used in this test is suspension of sheep


erythrocytes sensitized with a subagglutinating dose of rabbit
anti sheep erythrocyte antibody (amboreceptor).
ADVANTAGES
I. Simple visual reading, without expert
interpretation: bi-colored latex reaction
II. Fast results for clinicians (2 minutes)
III. Cost-effective, routine laboratory test
IV. Easy and ready-to-use
V. High sensitivity and specificity
Test principle

 When sheep red blood cells are coated with a


concentration of antiserum to sheep red blood cells
that is too low to cause agglutination, the addition of
serum from a patient with rheumatoid arthritis will
cause agglutination.
•REAG is composed of sheep red blood cells sensitized by
gamma globulins fraction of rabbit anti-sheep red blood cells
serum.

•The presence of Rheumatoid Factor in serum sample is


revealed by haemagglutination on a blue background, visible
to the naked eye.

•Otherwise, no agglutination is observed and the suspension


remains homogeneous and an uniform green-brown color.

•Handling is simple and fast. It is performed on pure serum


and he results are obtained in 2 minutes.
TEST PROCEDURE
1. Qualitative method
 Allow the reagents and samples to reach the room
temperature. The sensitivity of test may be reduced
at low temperature.
 Gently mix the WR reagent to obtain homogenous
suspension.
 Dispense 50µl of serum upon a selected spot of
reaction slide , add one drop of WR reagent and
acurately mix with a stirrer paying attention to
uniformly distribute the liquid on the selected spot.
Rotate the slide 2 or 3 times. Leave the slide in horizontal
position for 2 mins.

After this ti me, twist the slide once to about 45° from the
horizontal and let the slide again to stay on a flat surface 1
min more, and observe the possible agglutination.

2. Semi –quantitative method


 Make serial 2 fold dilution of the sample in the saline
solution.
 Proceed for each dilution as quantitative method
TEST RESULT AND INTERPRETATION
RESULT INTERPRETATION
 + ive :  Formation of red  Presence of Rheumatoid
agglutinates on a more or less Factor
blue intense background

 - ive: No agglutination. The  Absence of Rheumatoid


suspension remains Factor
homogeneous and purple
REFERENCE
•[Link] /

•[Link]
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THANK YOU

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