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    Microscope Care

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    Kate Blahblah
    The document provides instructions on handling, storing, cleaning, and using a microscope. It describes how to properly handle the microscope with two hands, being gentle to avoid damaging the lenses. When storing, the lowest power objective should be in place and the microscope covered. Cleaning involves using lens paper and avoiding facial tissues which could damage lenses. The parts of the microscope are defined, including the ocular, objectives, stage, and controls. Users are instructed to start with the lowest power objective and use the coarse then fine adjustments to focus. A coverslip should be used to avoid bubbles when preparing a slide.

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    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd

    Microscope Care

    Uploaded by

    Kate Blahblah
    199 views5 pages
    The document provides instructions on handling, storing, cleaning, and using a microscope. It describes how to properly handle the microscope with two hands, being gentle to avoid damaging the lenses. When storing, the lowest power objective should be in place and the microscope covered. Cleaning involves using lens paper and avoiding facial tissues which could damage lenses. The parts of the microscope are defined, including the ocular, objectives, stage, and controls. Users are instructed to start with the lowest power objective and use the coarse then fine adjustments to focus. A coverslip should be used to avoid bubbles when preparing a slide.

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    The document provides instructions on handling, storing, cleaning, and using a microscope. It describes how to properly handle the microscope with two hands, being gentle to avoid damaging the lenses. When storing, the lowest power objective should be in place and the microscope covered. Cleaning involves using lens paper and avoiding facial tissues which could damage lenses. The parts of the microscope are defined, including the ocular, objectives, stage, and controls. Users are instructed to start with the lowest power objective and use the coarse then fine adjustments to focus. A coverslip should be used to avoid bubbles when preparing a slide.

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    199 views5 pages

    Microscope Care

    Uploaded by

    Kate Blahblah
    The document provides instructions on handling, storing, cleaning, and using a microscope. It describes how to properly handle the microscope with two hands, being gentle to avoid damaging the lenses. When storing, the lowest power objective should be in place and the microscope covered. Cleaning involves using lens paper and avoiding facial tissues which could damage lenses. The parts of the microscope are defined, including the ocular, objectives, stage, and controls. Users are instructed to start with the lowest power objective and use the coarse then fine adjustments to focus. A coverslip should be used to avoid bubbles when preparing a slide.

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    of 5

    13

    Microscope Care & Use


    `
    Handling The Microscope
     Always use two hands to move the microscope. Place one hand
    around the arm, lift the scope, and then put your other hand
    under the base of the scope for support.
     Be gentle. Setting the microscope down on the table roughly
    could jar lenses and other parts loose. The microscope seems
    like a simple instrument, but each lens (eyepiece and objective)
    is actually made up of a number of other lenses; banging your
    microscope around, shakes about 15 to 20 lenses. Surprisingly, a
    good microscope can cost between $7000 and $15,000—so
    please be careful.
     Always have clean hands when handling your microscope.

    How would you carry the microscope?

    Storing The Microscope


     Return the lowest power objective in place
     Wrap the cord around the base
     Dust is an enemy to microscope lenses; always keep the
    microscope covered when not in use.

    Cleaning the Microscope


     Don’t let the microscope get too dirty – always use the dust
    cover when not in use.
     To clean the eyepiece – use a high quality lens paper. First brush
    any visible dust from the lens, and then wipe the lens. Do not use
    facial tissues, they are made from ground up wood fibers and
    could damage the lenses.
    14

     To clean the objective lenses – use a fresh piece of the lens paper
    each time so that you don't transfer dust from one lens to
    another.
     Use lens paper on all glass parts of the microscope.
     Clean oil immersion lens with chemicals provided by your
    instructor
    Parts of the Microscope
     Ocular-it’s the eyepiece; it’s where you look through to see the image of
    your specimen.
     Body tube-the long tube that holds the eyepiece and connects it to the
    objectives.
     Nosepiece-the rotating part of the microscope at the bottom of the body
    tube; it holds the objectives.
     Objective lenses- low (4x), medium (10x), high (40x), oil immersion(100x)
    The lens in the microscope that first receives light rays from the object and
    forms the image. The microscope may have 2, 3 or more objectives
    attached to the nosepiece; they vary in length (the shortest is the lowest
    power or magnification; the longest is the highest power or magnification).
    You will probably not be using the oil immersion lens, but if you do, you will
    need to place a drop of oil on the cover slip and slide the oil immersion
    objective into place. After use, you will also need to clean the objective to
    get the oil off.
     Arm-part of the microscope that you carry the microscope with.
     Coarse adjustment knob -large, round knob on the side of the
    microscope used for focusing the specimen; it may move either the stage
    or the upper part of the microscope.
     Fine adjustment knob-small, round knob on the side of the microscope
    used to fine-tune the focus of your specimen after using the coarse
    adjustment knob.
     Stage-large, flat area under the objectives; it has a hole in it (see
    aperture) that allows light through; the specimen/slide is placed on the
    stage for viewing.
     Stage clips- a metallic clip on top of the stage that holds the slide in
    place.
     Aperture-the hole in the stage that allows light through for better viewing
    of the specimen.
     Diaphragm-controls the amount of light going through the aperture.
     Light -source of light usually found near the base of the microscope; the
    light source makes the specimen easier to see.
     Base – Supports the microscope
    15

    Microscope Parts
    A. ocular E. objective I. lever to move stage clip M. base
    B. body tube F. arm J. course adjustment
    C. stage clip G. stage K. fine adjustment
    D. revolving nose piece H. diaphragm L. light source
    16

    Using the Microscope


     Always observe the specimen or object using the LOWEST POWER
    object first.
     Focus using the COARSE ADJUSTMENT KNOB to bring the object into
    focus. Bring the object into sharp focus by using the fine adjustment knob.
     Focus, and then move to a higher power objective, if needed.
     Use only the FINE ADJUSTMENT KNOB when using the HIGHEST
    (longest) POWER OBJECTIVE.
     Keep both eyes open to reduce eyestrain. Keep eye slightly above the
    eyepiece to reduce eyelash interference.
     To find out the total magnification of the object, multiply the power of the
    eyepiece lens (10X) by the power of the objective.

    Preparing a Slide
    1. Using a pipet or dropper, add a drop of water
    or another solvent to a clean microscope slide. Then, place the specimen
    in the water.
    2. Place the edge of a coverslip on the slide so
    that it touches the edge of the water.
    3. Slowly lower the coverslip to prevent the
    formation of air bubbles.
    17

    Quiz

    1. Which objective uses oil?

    2. If your ocular is 10x and your objective is 40x what is the total
    magnification of your image?

    3. Why do you need a coverslip and how do you avoid air bubble?

    4. What is the difference between a light microscope, dissecting microscope


    and electron microscope?

    5. Who invented the microscope?

    6. When storing the microscope, what objective should be locked in place?

    7. Which focus knob provides a sharper image?

    Matching
    _____ ocular A. supports the slide
    _____ body tube B. roughly brings specimen into focus
    _____ stage clip C. eye piece
    _____ diaphragm D. connects the ocular to objective
    _____ course adjustment E. rotates objectives
    _____ fine adjustment F. holds slide in place
    _____ base G. hold it when transporting the microscope
    _____ objective H. makes light to see the specimen
    _____ stage I. adjust image for fine detail
    _____ arm J. controls the amount of light through the
    aperture
    _____ revolving nose piece K. a lens that receives light from the object and
    forms the image
    _____ light source L. Supports the microscope

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