INSTRUCTIONS

CX22LED
BIOLOGICAL MICROSCOPE

This instruction manual is for the Olympus Biological Microscopes Model CX22LED. To ensure the
safety, obtain optimum performance and to familiarize yourself fully with the use of this microscope,
we recommend that you study this manual thoroughly before operating the microscope. Retain this
instruction manual in an easily accessible place near the work desk for future reference.
AX8002
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NOTE: This equipment has been tested and found to comply with the limits for a Class A digital device,
pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection
against harmful interference when the equipment is operated in a commercial environment. This
equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in
accordance with the instruction manual, may cause harmful interference to radio communications.
Operation of this equipment in a residential area is likely to cause harmful interference in which case
the user will be required to correct the interference at his own expense.

FCC WARNING: Changes or modifications not expressly approved by the party responsible for compliance
could void the user’s authority to operate the equipment.

In accordance with European Directive 2002/96/EC on Waste Electrical and Electronic Equipment,
this symbol indicates that the product must not be disposed of as unsorted municipal waste, but
should be collected separately.
Refer to your local Olympus distributor in the EU for return and/or collection systems available in
your country.

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CX22LED
CONTENTS
IMPORTANT — Be sure to read this section for safe use of the equipment. — 1-3

1 Intended Use ........................................................................................................................................................................................................................... 2

2 Getting Ready ........................................................................................................................................................................................................................ 2
3 Maintenance and Storage ......................................................................................................................................................................................................................... 3

1 STANDARD COMPONENT UNITS 4

2 NOMENCLATURE 5

3 SUMMARY OF BRIGHTFIELD OBSERVATION PROCEDURE 6

4 DETAILED OBSERVATION PROCEDURE 7-11

1 Turning the LED Illumination ON .......... 7 2 Placing Specimen on the Stage .................... 7
3 Adjusting the Focus ................................................ 8 4 Adjusting the Interpupillary Distance ..................... 9
5 Adjusting the Diopter ........................................ 10
6 Adjusting the Condenser Position and Aperture Iris Diaphragm ............................................................. 10
7 Switching the Objectives ............................ 10 8 Using the 100X Immersion Objective .................... 11

5 ONE-POINT ADVICE 12

6 TROUBLESHOOTING GUIDE 13,14

7 SPECIFICATIONS 15

8 OPTICAL CHARACTERISTICS 16

9 ASSEMBLY 17

10 OPTIONAL ACCESSORIES 18-20

10-1 System Diagram of Optional Accessories ............................................................................ 18

10-2 Installation and Operation of Optional Accessories .............................................. 18
1 Filter Holder CH2-FH and Darkfield Ring CH2-DS ........................................................................................................ 18
2 Reflection Mirror CH20-MM ........................................................................................................................................................................... 19
3 Eyepieces WHC15X/WHC15X-H ............................................................................................................................................................................................... 19
4 Using the eyepiece micrometer ............................................................................................................................................................................................... 20
5 Cord Hanger CX22LED-CH ................................................................................................................................................................................................................. 20

PROPER SELECTION OF THE POWER CORD .................................................................................. 23,24

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IMPORTANT

SAFETY PRECAUTIONS

1. After the equipment has been used in an observation of a specimen that is accompanied with a potential of
infection, clean the parts coming in contact with the specimen to prevent infection.
· Moving this product is accompanied with the risk of dropping the specimen. Be sure to remove the specimen before
moving this product.
· In case the specimen is damaged by erroneous operation, promptly take the infection prevention measures.
2. Install the microscope on a sturdy, level table or bench.
3. Always use the AC adapter and power cord provided by Olympus. If no power cord is provided, please select the proper
power cord by referring to the section “PROPER SELECTION OF THE POWER CORD” at the end of this instruction
manual. If the proper power cord is not used, product safety performance cannot be warranted.
4. Always ensure that the grounding terminal of the microscope and that of the wall outlet are properly connected. If the
equipment is not grounded, Olympus can no longer warrant the electrical safety performance of the equipment.
5. After operation or in case of abnormality, be sure to disconnect the power cord of the AC adapter from the connector on
the microscope or from the wall power outlet.
6. This product complies with the emission and immunity requirements described in IEC61326 series.
7. The electromagnetic environment should be evaluated prior to operation of this product. Do not use this product in close
proximity to the sources of strong electromagnetic radiation to prevent interference with the proper operation.

LED (light emitting diode) safety warning

The LED built in this product is basically eye-safe. However, direct viewing the light from the LED unit for extended time
may cause damage to the eyes.

Safety Symbols
The following symbols are found on the microscope. Study the meaning of the symbols and always use the equipment
in the safest possible manner.

Symbol Explanation
Before use, carefully read the instruction manual. Improper use could result in personal injury to
the user and/or damage to the equipment.

Indicates that the main switch is ON.

Indicates that the main switch is OFF.

Caution
If the microscope is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition,
the equipment may also be damaged. Always use the equipment as outlined in this instruction manual.

The following indications are used to set off text in this instruction manual.
CAUTION : Indicates a potentially hazardous situation which, if not avoided, may result in minor
or moderate injury or damage to the equipment or other property. It may also be used
to alert against unsafe practices.
} : Indicates commentary (for ease of operation and maintenance).

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CX22LED

1 Intended use
This product has been designed to be used to observe magnified images of specimens in various routine work and
research applications.
Do not use this product for any purpose other than its intended use.

This product complies with the requirements of directive 98/79/EC concerning in vitro diagnostic
medical devices. CE marking means the conformity to the directive.

USA CAUTION:
Federal law restricts this device to sale by or on the order of an appropriately licensed healthcare practitioner.

2 Getting Ready
1. A microscope is a precision instrument. Handle it with care and avoid
² subjecting it to sudden or severe impact.
2. Do not use the microscope where it is subjected to direct sunlight,
high temperature and humidity, dust or vibrations. (For the operating
conditions, see chapter 7, “SPECIFICATIONS” on Page 15.)
3. Always use the tension adjustment ring to adjust the rotation tension of
@ the coarse adjustment knob.
4. Be sure to leave enough space around the AC adapter when installing
the microscope.
5. When carrying the microscope, first make sure that the AC adapter has
been disconnected and hold both sides around the hole of the arm as
Fig. 1
shown in Fig. 1 and carry it carefully.
CAUTION · To prevent damage, do not hold the microscope by the
stage 1 or observation tube 2.
Be sure to remove the specimen; otherwise, it may fall.
· Do not carry the microscope with the AC adapter connected
to prevent damage to the AC adapter or the microscope
by hitting the output connector of the AC adapter.
· Be sure to disconnect the AC adapter from the microscope
when storing the microscope after use.

Disconnect the AC adapter before

carrying or storing the microscope.

Fig. 2

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3 Maintenance and Storage
1. To clean the lenses and other glass components, simply blow away dirt using a commercially available blower and wipe
the components gently using a piece of cleaning paper (or clean gauze).
If a lens is stained with fingerprints or oil smudges, wipe it with gauze slightly moistened with commercially available
absolute alcohol.
CAUTION Since the absolute alcohol is highly flammable, it must be handled carefully.
Be sure to keep it away from open flames or potential sources of electrical sparks –– for example, electrical
equipment that is being switched on or off, since such operation may cause ignition of a fire.
Also remember to always use it only in a well-ventilated room.
2. Do not attempt to use organic solvents to clean the microscope components other than the glass components. To clean
them, use a lint-free, soft cloth slightly moistened with a diluted neutral detergent.
3. Do not disassemble any part of the microscope as this could result in malfunction or reduced performance.
4. When not using the microscope, store it in a dry locker or cover it with a dust cover.

5. To clean the condenser, fully loosen the securing knob 1, then remove
the condenser by lowering it with the condenser height adjustment knob
2, and wipe the front lens of the condenser.
The condenser can be attached by reversing the above removal
² procedure (Fig. 3).
@ 6. When disposing of the microscope, check the regulations and rules
of your local government and be sure to observe them.

Fig. 3

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CX22LED
STANDARD COMPONENT UNITS
}After opening the package, make sure that the correct component units for the selected set are included.
The 100X objective and immersion oil are provided only with the CX22LEDRFS1 set.
CAUTION The objectives have been screwed in tightly to prevent them from being loosened during transportation.
To remove an objective, turn it counterclockwise while holding it with a rubber sheet, etc., so that your
fingers don’t slip.

Binocular observation
tube Objectives
· 4X, 10X, 40X, 100X
(CX22LEDRFS1)
· 4X, 10X, 40X
(CX22LEDRFS2)

Eyepieces Microscope frame

(Fixed at 10X)

Power cord

Revolving nosepiece

Stage

Condenser

AC adapter

Immersion oil
· Provided with a set including
the 100X objective.

Optional Accessories
· Filter Holder · Eyepieces · Cord Hanger
CH2-FH WHC15X CX22LED-CH
WHC15X-H

· Reflection Mirror · Darkfield Ring

CH20-MM CH2-DS

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NOMENCLATURE

Observation tube clamping

knob

Pre-focusing knob (Page 9)

Diopter adjustment ring
(Page 9)

Revolving nosepiece
(Page 10)
Coarse adjustment
knob (Page 8)

Specimen holder (Page 7)

Fine adjustment
knob (Page 8)

Aperture iris diaphragm ring

(Page 10)

Specimen holder X-axis

feed knob (Page 7)
Filter holder
Accommodates a
45-mm diameter filter.

Main switch (Page 7)

Specimen holder Y-axis
feed knob (Page 7) “ ”: Power ON
“ ”: Power OFF
Light intensity adjustment
knob (Page 7)

}Attach the AC adapter and power cord as described in chapter 9, “ASSEMBLY” on page 17.
}For detailed description of each item, refer to the page indicated inside parentheses.

NOTE FOR UNPACKING

}The following items have been attached at the factory to prevent deterioration during transport. Remove these items and
retain them for future use. Then loosen the observation tube clamping knob and correct the orientation of the eyepieces
as shown in the illustration below.
1 Revolving nosepiece/observation tube transport band
2 Stage and specimen holder protection sheet
3 Protective pad below the stage

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CX22LED
SUMMARY OF BRIGHTFIELD OBSERVATION PROCEDURE

(Items Used) (Page)

Set the main switch to “ I " (ON) and adjust 1Main switch (P. 7)
the brightness. 2Light intensity adjustment knob (P. 7)

3Specimen holder (P. 7)

Place the specimen on the stage.
4X-axis/Y-axis feed knobs (P. 7)

Engage the 10X objective in the light path. 5Revolving nosepiece (P. 10)

Bring the specimen in focus 6Coarse/fine focus adjustment knobs (P. 8)

Adjust the interpupillary distance. 7Binocular tube (P. 9)

Adjust the diopter. 8Diopter adjustment ring (P. 9)

Adjust the aperture iris diaphragm. 9Aperture iris diaphragm ring (P. 10)

Engage the objective to be used in the light 5Revolving nosepiece (P. 10)
path and bring the specimen in focus. 6Coarse/fine adjustment knobs (P. 8)

Engage the required filters. aFilters (Diameter: 45 mm) (P. 5)

Adjust the brightness. 2Light intensity adjustment knob (P. 7)

Adjust the aperture iris diaphragm. 9Aperture iris diaphragm ring (P. 10)

Start observation.

7
5
6
3

a
1
4 2

6
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DETAILED OBSERVATION PROCEDURE

1 Turning the LED Illumination ON (Fig. 4)

1. Set the main switch 1 to “ I ” (ON).

2. Rotating the light intensity adjustment knob 2 in the direction of the
arrow increases brightness and rotating it in the opposite direction de-
creases brightness. The figures around the knob indicate the reference
brightness.

@
²

Fig. 4

2 Placing Specimen on the Stage (Figs. 5 & 6)

@ 6 CAUTION Place the specimen gently. If the bow-shaped lever is

returned with a strong force or the knob 1 of the bow-
²
shaped lever is released in the middle, the slide glass
may be broken.
³ 1. Rotate the coarse adjustment knob 2 in the direction of the arrow to fully
lower the stage.
2. Open the bow-shaped lever 3 outward, place the specimen by sliding
the specimen glass plates on the stage from the front toward the rear.
|
3. After sliding the specimen glass plates all the way, return the bow-shaped
5
lever 3 gently.
Fig. 5 4. Rotating the upper knob which is the Y-axis feed knob 4 moves the
specimen in the vertical direction. Rotating the lower knob which is the X-
axis feed knob 5 moves it in the horizontal direction.
CAUTION · Do not move the specimen holder 6 directly by hand,
this will damage the rotary mechanisms of the above
knobs.
· When the specimen holder reaches the stopper position,
the rotation force of the above knobs become heavy.
Stop rotating the knob at this time.

Cover glass Cover glass

This is the glass plate placed on the specimen. To allow the objective
manifest the full performance, the cover glass thickness, which is the
distance from its surface to the specimen surface, should ideally be
0.17 mm.
Slide glass
Slide glass
Placing Specimen
This glass plate should ideally have a length of 76 mm, width of 26
Fig. 6 mm and thickness between 0.9 and 1.4 mm.

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CX22LED

Specimen holder scales (Fig. 7)

@
}Theses scales allow the position (coordinates) being observed on the
specimen to be identified. Even after the specimen is moved, it can be
returned easily to the original position.
1. The horizontal coordinate can be read at position 1 on the specimen
holder.
² 2. The vertical coordinate can be read at the position of index line 2.

Fig. 7

3 Adjusting the Focus (Fig. 8)

Focusing Procedure
³ 1. Rotate the coarse adjustment knob @ in the direction of the arrow so
that the objective 3 is as close as possible to the specimen.
2. While observing the specimen through the eyepieces, slowly rotate the
@ coarse adjustment knob 1 in the opposite direction to the arrow to lower
the stage.
3. When coarse focusing of the specimen is obtained, rotate the fine
adjustment knob 2 to adjust to precise focus.
²
Working Distance (WD)
Fig. 8
}The WD refers to the distance between each objective and the specimen,
when precise focus of the specimen is obtained.

Objective
Magnification 4X 10X 40X 100X

WD (mm) 27.8 8.0 0.6 0.13

Adjusting the Tension of the Coarse Adjustment Knob (Fig. 9)

@
1. The tension of the coarse focus adjustment knob has been designed
³ adjustable with a ring. Insert the tip of a large flat-blade screwdriver into
the groove 2 on the tension adjustment ring 1 and rotate the ring.
Rotating it clockwise (in the direction of the arrow) increases the tension
and counterclockwise decreases the tension.
² 2. If the stage descends on its own or if the specimen gets out of focus
quickly even when it is brought into focus using the fine adjustment
knob, it means that the tension of the coarse adjustment knob is too low.
Turn the ring 1 in the direction of the arrow to increase the tension.
Fig. 9

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Pre-focusing Knob (Fig. 10)
@
}The pre-focusing knob controls the mechanism for preventing collision
between the specimen and objective.
1. After bringing the specimen into focus, turn the pre-focusing knob 1
inside the hole of the arm so that the pre-focusing mechanism hits the
stage guide.
2. To provide a certain margin for focusing, rotate the knob by about half
turn backward from the stopped position.
CAUTION If the function of this mechanism is not required, set the
pre-focusing knob 1 at the highest position. If the pre-
focusing knob 1 is not set at the highest position, the
Fig. 10
specimen may not be focused.

4 Adjusting the Interpupillary Distance (Fig. 11)

}The interpupillary distance adjustment is to regulate the two eyepieces

according to that between your eyes so that you can observe a single
microscopic image through two eyepieces. This helps to reduce fatigue
during observation.
While looking through the eyepieces, move both eyepieces until the left
and right fields of view coincide completely.
When the both of eyepieces are lined in the horizontal position, the posi-
tion of index ridge on the right side eyepiece sleeve 1 indicates the
interpupillary distance value.
}Note your interpupillary distance so that it can be quickly duplicated.
}You can move the eyepieces upward and downward from the position
where both eyepieces are in a straight line with maximum interpupillary
distance.

@
Fig. 11

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CX22LED

5 Adjusting the Diopter (Fig. 12)

}The diopter adjustment is to compensate for the difference in eyesight

between your eyes.
1. While looking through the right eyepiece with your right eye, rotate
the coarse and fine focus adjustment knobs to bring the specimen
into focus.
2. While looking through the left eyepiece with your left eye, rotate only the
diopter adjustment ring 1 to focus on the specimen.

Fig. 12

Using the Eye Shades (Fig. 13)

When Wearing Eyeglasses

Use the eye shades in the normal, folded-down position. This will prevent
the eyeglasses from being scratched.

When Not Wearing Eyeglasses

Extend the folded eye shades in the direction of the arrow to prevent
extraneous light from entering between the eyepieces and eyes.

Fig. 13

6 Adjusting the Condenser Position and Aperture Iris Diaphragm (Fig. 14)

}The condenser is usually used in the highest position. If the entire observed
field of view is not bright enough, brightness may be improved by lowering
the condenser slightly.
1. Rotate the condenser height adjustment knob 1 to move the condenser
to the highest position.
2. The aperture iris diaphragm ring 2 has an objective magnification
scale (4X, 10X, 40X, 100X). Rotate the ring so that the magnification
of the objective in use faces frontward.
@ ²

Fig. 14

7 Switching the Objectives (Fig. 15)

Hold and rotate the revolving nosepiece 1 so that the objective to be

used comes exactly above the specimen.
@

Fig. 15

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8 Using the 100X Immersion Objective (Fig. 16)

}The designated immersion oil should be applied to the top lens of the
100X immersion objective. Otherwise, the observed image will be unable
to be focused on.
CAUTION Always use the immersion oil supplied by Olympus.
1. Focus on the specimen using all objectives, starting from the lowest-
power objective to higher-power objective.
2. Before engaging the immersion objective in the light path, place a drop of
the provided immersion oil onto the specimen at the area to be observed.
3. Rotate the revolving nosepiece to engage the immersion objective and
rotate the fine adjustment knob to bring the specimen into focus.
Fig. 16 CAUTION Since air bubbles in the oil will affect the image quality,
make sure that the oil is free of bubbles.
To remove bubbles, rotate the revolving nosepiece slightly
to move the oil-immersed objective by one reciprocation
or two.
}The condenser of this microscope manifests the full performance when
oil is placed between the slide glass and the front lens of condenser. If oil
is not applied there, the observation image may become slightly dark.
4. After use, remove oil from the objective front lens by wiping with gauze
slightly moistened with absolute alcohol.

CAUTION Caution in use of immersion oil

If immersion oil enters your eyes or contacts your skin,
immediately take the following treatment.
Eyes: Rinse with fresh water (for 15 minutes or more).
Skin : Rinse with water and soap.
If the appearance of the eyes or skin is altered or pain
persists, immediately see your doctor.

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CX22LED
ONE-POINT ADVICE

How To Track a Microscopic Image

To track an image
moving leftwards Move the slide
(or to move the glass to the
specimen image left.
rightwards):

Direction of moving the image

To move the
Move the slide
specimen image
glass down.
upward:

}The image observed through the microscope moves in

directions opposite to the actual up-down and left-right
movements of the specimen.

Total Magnification

The size of the specimen image for observation is obtained by multiplying the eyepiece magnification by the
objective magnification. This value is referred to as total magnification.
Example: Eyepiece (10X) x Objective (40X) = 400X

Resolution

Resolution determines how finely a lens is able to distinguish the details of a specimen.
The resolution of a microscope is mainly determined by the ability of the objective and is scarcely related to that of the
eyepieces. The only function of the eyepieces is to magnify an image already resolved by the objective.
Although both configurations above provide the same total magnification, the higher magnification of the objective
yields a better specimen resolution.

Field Number (FN)

The field number is the diameter of the image observed through an eyepiece, represented in millimeters.
10X eyepiece: 20 mm WHC15X: 12 mm Approx. 1.0 mm,
about half of actual
field of view.
Actual Field of View

The actual field of view is the size on the specimen actually

observed in the microscope. This yields approximate size of
the actual specimen.

Actual field of view = Field number/Objective magnification

When the 10X eyepiece above and 10X objective are used,
the actual field of view is equal to:

Actual field of
Actual field of view = 20/10 = 2.0 mm view: 2.0 mm

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TROUBLESHOOTING GUIDE
Under certain conditions, performance of the unit may be adversely affected by factors other than defects. If problems occur,
please review the following list and take remedial action as needed. If you cannot solve the problem after checking the entire list,
please contact Olympus for assistance.

Problem Cause Remedy Page

1. Uneven brightness in observation The objective is not engaged in the light Engage the objective into position until
10
field. path. it clicks.
The condenser is too low. Raise it to the upper limit. 10
The objective, eyepiece, condenser and/ Clean them thoroughly.
3
or window lens are dirty.
2. Dust or stains are visible in obser- The eyepiece, condenser, window lens Clean them thoroughly.
3
vation field. and/or specimen glasses are dirty.
3. Observation image glares. The condenser is too low. Raise it. 10
The condenser iris diaphragm ring is Adjust the aperture according to the ob-
10
stopped down excessively. jective magnification.
4. Observation image is whitish- The objective is not engaged in the light Engage the objective into position until
10
blurred or unclear. path. it clicks.
The objective, eyepiece, condenser and/ Clean them thoroughly.
3
or specimen glasses are dirty.
Immersion oil is not used with an immer- Use immersion oil.
11
sion objective.
Bubbles are mixed in the immersion oil. Remove the bubbles. 11
The specified immersion oil is not used. Use the immersion oil supplied by
11
Olympus.
5. Part of image is defocused or image The objective is not properly engaged in Engage the objective into position until
10
looks like it’s flowing. the light path. it clicks.
The specimen is not set properly on the Set the specimen correctly on the stage
7
stage. and secure using the specimen holder.
6. High-magnification objective The specimen is upside down. Set the specimen correctly with the
touches specimen just before cover glass on the top. 7
coming into focus.
7. The tension of coarse adjustment The coarse adjustment knob tension ad- Loosen the ring to adjust to proper
8
knob it too high. justment ring is set too tight. tension.
8. Focusing is impossible (because The pre-focusing knob is positioned too Raise its position.
9
the stage cannot be raised). low.
9. The stage lowers by its own weight The coarse adjustment knob tension Tighten the ring to adjust to proper ten-
or focusing is lost due to slippage adjustment ring is set too loose. sion. 8
of the knob.
10. Coarse focus adjustment cannot The condenser is too low. Raise it.
10
lower the stage low enough.
11. Fields of view of two eyes do not The interpupillary distance is not adjusted Adjust it properly.
9
match. properly.
Diopter compensation for the two eyes is Adjust it correctly.
10
not set.
The left and right eyepieces are different. Replace one of them so that the left
--
and right eyepieces are identical.

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CX22LED

Problem Cause Remedy Page

12. Objective hits the specimen when The specimen is upside down. Set the specimen correctly with the
7
an objective is switched to a higher- cover glass on the top.
magnification objective. The cover glass is too thick. Use a cover glass with thickness of
7
0.17 mm.
13. The LED illumination does not light. The AC adapter or power cord is un- Plug it securely.
17
plugged.

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SPECIFICATIONS

Item Specifications
1. Optical system Infinity Correction optical system
2. Illumination Built-in 0.5 W LED illumination system.
Rated voltage: 5-6 V 0.5 A
(AC adapter: 100-240 V 50/60 Hz 0.4A)
Power consumption: 1.7 W
3. Focusing mechanism Stage height adjustment mechanism.
Fine adjustment scale: 2.5 μm per graduation
Fine adjustment stroke: 0.3 mm per turn
Total stroke: 20 mm.
Pre-focusing knob provided, coarse adjustment knob tension adjustable.
4. Revolving nosepiece Quadruple positions fixed (Front oriented)
5. Binocular observation tube Field number 20
Tube tilting angle 30°
Interpupillary distance 48 to 75 mm
adjustment range
6. Stage Size 120 x 132 mm (with mechanical stage)
Movement range 76 (X-axis) x 30 (Y-axis) mm
Specimen holder Holds a single specimen.

7. Condenser Type Abbe condenser

N. A . 1.25 (when immersed in oil)
Aperture iris diaphragm Built in
8. Dimensions & weight 154(W) x 392(H) x 238(D) mm, approx. 6 kg
9. Operating environment · Indoor use.
· Altitude: Max. 2000 meters
· Ambient temperature: 5° to 40°C (41° to 104° F)
· Maximum relative humidity: 80% for temperatures up to 31°C (88°F),
decreasing linearly through 70% at 34°C
(93°F), 60% at 37°C (99°F), to 50% relative
humidity at 40°C (104°F).
· Supply voltage fluctuations; Not to exceed ±10% of the normal voltage.
· Pollution degree: 2 (in accordance with IEC60664-1)
· Installation/Overvoltage category: II (in accordance with IEC60664-1)
10. Transport and Storage environ- · –25 to +65 °C
ment · 0 to 90 %RH

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CX22LED
OPTICAL CHARACTERISTICS
The following table shows the optical characteristics of com-
binations of eyepieces and objectives. The figure on the right Magnification
shows the performance data engraved on the objectives.
Mechanical tube Numerical aperture
length

Field number
Color band

Cover glass
Objective type (on the back side) thickness
Plan Objective

Optical 10X Eyepieces (FN 20)

Characteristics Cover
Magnifi- W.D. Glass Resolution Focal Actual
N.A . (mm) Thickness (μm) Total Remark
cation Depth Field of
(mm) Power (μm) View
Objectives

Plan objective (FN 20) 4X 0.10 27.8 – 3.36 40X 175.0 5.0
10X 0.25 8.0 – 1.34 100X 28.0 2.0
40X 0.65 0.6 0.17 0.52 400X 3.04 0.5
100XO* 1.25 0.13 – 0.27 1000X 0.69 0.2 Oil immersed

* 100XO (FN 20)

Legend

Working distance (WD) : Distance between the top surface of the cover glass and the objective extremity.
Number of aperture (NA) : The figure corresponding to the F-number of the camera. This is associated with the resolution
and larger NA means higher resolution.
Resolution : Ability of an objective for identifying adjacent two lines in the image, which is expressed in terms
of the minimum distance between two points on the specimen surface.
Focal depth : The depth range of a specimen, in which focusing is obtained at a time. Stopping down the
(Object side) : aperture iris diaphragm increases the focal depth and increasing the objective NA decreases it.
Field number (FN) : The diameter of the image observed through an eyepiece, represented in millimeters.
Actual field of view : Diameter of the field of view, expressed as the size on the specimen surface.
Total power : Objective magnification x Eyepiece magnification.
(Total magnification)

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ASSEMBLY

}Each standard set can be assembled by simply attaching the AC adapter and power cord.

Connecting the AC Adapter and

1 Power Cord (Figs. 17 & 18)

CAUTION · Be sure to use the provided AC adapter. Use of any other AC

adapter may cause a malfunction.
² · The power cord and AC adapter cord are vulnerable when
bent or twisted. Never subject them to excessive force.
· Make sure that the main switch 1 is set to “ ” (OFF) before
@ connecting the power cord.
³ · Always use the power cord provided by Olympus. If no power
5 cord is provided, please select the proper power cord by
| referring to section “PROPER SELECTION OF THE POWER
CORD” at the end of this instruction manual.
1. Connect the output connector 2 of the AC adapter to the input connec-
tor 3 on the rear of the microscope.
Fig. 17
CAUTION Do not tilt the microscope to the rear. Doing so could damage
the output connector 2.
2. Connect the power cord’s connector 4 to connector 5 firmly.
6 7 CAUTION Be sure to supply power from a grounded, 3-conductor power
outlet using the proper power cord. If the power outlet is not
grounded properly, Olympus can no longer warrant the electrical
safety performance of the equipment.
3. Connect the power cord’s plug 6 to a wall power outlet 7. (Fig. 18)

* Shapes of the power cord’s plugs and

power outlets are different by country.

Fig. 18

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CX22LED
OPTIONAL ACCESSORIES

10-1 System Diagram of Optional Accessories

Eyepieces
WHC15X
WHC15X-H
Eyepiece
micrometer
Reticle holder
20.4RH

Cord Hanger
CX22LED-CH
Filter Holder Microscope Frame
CH2-FH CX22LED

Darkfield Ring
CH2-DS Reflection Mirror
CH20-MM 45 mm filter

10-2 Installation and Operation of Optional Accessories

1 Filter Holder CH2-FH and Darkfield Ring CH2-DS (Fig. 19)

}This holder accommodates the CH2-DS darkfield ring.

1. Insert the darkfield ring @ in the CH2-FH filter holder 2.
2. Push the filter holder 2 containing the darkfield ring @ into the bottom of
the condenser until it clicks into place.
@ }The darkfield ring enables darkfield observation using an objective from
4X to 40X.

Fig. 19

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2 Reflection Mirror CH20-MM (Fig. 20)

}The reflection mirror is designed to be used in microscopic observation in a location where power supply is not available.
It makes it possible to use the natural light in place of the LED illumination.

CAUTION The microscope should be installed near a window, in a

place that is not exposed to direct sunlight.
The eyepieces should be oriented toward the rear so
that the reflection mirror receives the bright light. Loosen
the observation tube clamping knob and rotate the tube
by 180°.
1. Fit the reflection mirror in the window lens of the microscope frame by
@ aligning the mounting tab.
2. The reflection mirror 1 should be pointed toward the bright area. While
observing the image through eyepieces, adjust the orientation of the
Fig. 20 reflection mirror.
}A planar reflection mirror is used normally. However, if the image bright-
ness is uneven or the outside view is visible in the image, use a concave
reflection mirror.

3 Eyepieces WHC15X/WHC15X-H (Fig. 21)

}The standard 10X eyepieces are clamped using screws.

1. Using a small flat-blade screwdriver, loosen the clamping screw 1 of a
10X eyepiece and remove it.
2. Insert the WHC15X or WHC15X-H into the eyepiece sleeve and tighten
the clamping screw 1.

Fig. 21

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CX22LED

4 Using the Eyepiece Micrometer Disk (Optional) (Fig. 22)

}Prepare one eyepiece micrometer disk (diameter 20.4 mm, thickness 1

mm) and two 20.4-RH reticle holders (available as 2-piece set).
@ The field number becomes 19.6 when the reticle holders are used.
If you are using WHC15X or WHC15X-H, please purchase the micrometer
with 19mm diameter and 1mm thickness.
}As the helicoid adjustment is not available for the eyepieces other than
² WHC15X-H, if your eye is poor in eyesight and cannot focus the micrometer,
use eyeglasses to correct your vision.
1. Remove both eyepieces by loosening the clamping screw using a
small flat-blade screwdriver.
3 2. Hold the micrometer disk @ with the surface with indications facing down
Fig. 22 and place it into a reticle holder ².
CAUTION Be careful not to leave dirt on the micrometer disk, as it
will be noticeable during observation.
3. Screw the reticle holder ² containing the micrometer disk @ into the
bottom of an eyepiece.
Be sure to screw in all the way by hooking your nail into the notch 3
on the holder at the end.
4. Screw the other reticle holder, alone, into the other eyepiece in order to
align the field number.
5. Attach the eyepieces again, and tighten the clamping screw.

5 Cord Hanger CX22LED-CH (Figs. 23 to 29)

Attachment
@ }When the cord hanger (CX22LED-CH) is attached on the back of the
microscope frame, the power cord and AC adapter can be wound around
it for storing.
1. Align the hooks ² of the cord hanger @ with the cord hanger mounting
² position 3, insert the cord hanger, push it firmly against the microscope
frame and slide it down to lock it.

Fig. 23

Storage
1. Insert the connector @ of the power cord firmly into the input connector
² of the AC adapter (Fig. 24).
}Be sure to unplug the power cord from the outlet in advance.

² @

Fig. 24

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2. Place the AC adapter 3 on top a of the cord hanger 4 (Fig. 25).
³

Fig. 25

3. Bring the cable 5 of the AC adapter into the hole of center part b of
cord hanger. Then, bring it down so that it goes around the bottom
part c of cord hanger and bring it into the hole b again (Fig. 26).

Fig. 26

4. Insert the connector 6 of the AC adapter firmly into the connector 7 of

the microscope (Fig. 27).

7 6

Fig. 27

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CX22LED

5. Place the power cord ‡ on the cord hanger 4 as illustlated (Fig. 28).

Fig. 28

Removal
9 1. Insert the tool 9 (such as screwdriver) into the space between the pin a
of cord hanger 4 and the microscope.
a 2. Keep the tool 9 in the space and pull it approx. 10 mm (in the direction
1 ). Then take the cord hanger 4 up (in the direction 2 ) to remove
from the microscope.

|
Fig. 29 CAUTION · Do not carry the microscope by holding it by the cord
hanger. If it is detached during transport, the microscope
may drop and human injury may result.
· Be sure to disconnect the AC adapter from the
microscope when storing the microscope after use.

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PROPER SELECTION OF THE POWER CORD
If no power cord is provided, please select the proper power cord for the equipment by referring to “ Specifications ” and “ Certified
Cord ” below:
CAUTION: In case you use a non-approved power cord for Olympus products, Olympus can no longer warrant the
electrical safety of the equipment.

Specifications

Voltage Rating 125V AC (for 100-120V AC area) or, 250V AC (for 220-240V AC area)
Current Rating 6A minimum
Temperature Rating 60°C minimum
Length 3.05 m maximum
Fittings Configuration Grounding type attachment plug cap. Opposite terminates in molded-on IEC con-
figuration appliance coupling.

Table 1 Certified Cord

A power cord should be certified by one of the agencies listed in Table 1 , or comprised of cordage marked with an agency
marking per Table 1 or marked per Table 2. The fittings are to be marked with at least one of agencies listed in Table 1. In
case you are unable to buy locally in your country the power cord which is approved by one of the agencies mentioned in
Table 1, please use replacements approved by any other equivalent and authorized agencies in your country.

Certification Certification
Country Agency Country Agency
Mark Mark

Argentina IRAM Italy IMQ

Australia SAA Japan JET, JQA, TÜV,

UL Japan / METI

Austria ÖVE Netherlands KEMA

Belgium CEBEC Norway NEMKO

Canada CSA Spain AEE

Denmark DEMKO Sweden SEMKO

Finland FEI Switzerland SEV

France United ASTA

UTE Kingdom BSI

Germany VDE U.S.A. UL

Ireland NSAI

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CX22LED

Table 2 HAR Flexible Cord

APPROVAL ORGANIZATIONS AND CORDAGE HARMONIZATION MARKING METHODS

Printed or Embossed Harmoniza- Alternative Marking Utilizing

tion Marking (May be located on Black-Red-Yellow Thread (Length
Approval Organization jacket or insulation of internal wir- of color section in mm)
ing) Black Red Yellow
Comite Electrotechnique Belge
(CEBEC) CEBEC <HAR> 10 30 10

Verband Deutscher Elektrotechniker

(VDE) e.V. Prüfstelle <VDE> <HAR> 30 10 10

Union Technique de l´Electricite´

(UTE) USE <HAR> 30 10 30

Instituto Italiano del Marchio di

Qualita´ (IMQ) IEMMEQU <HAR> 10 30 50

British Approvals Service for Electric

Cables (BASEC) BASEC <HAR> 10 10 30

N.V. KEMA KEMA-KEUR <HAR> 10 30 30

SEMKO AB Svenska Elektriska

SEMKO <HAR> 10 10 50
Materielkontrollanstalter

Österreichischer Verband für

<ÖVE> <HAR> 30 10 50
Elektrotechnik (ÖVE)

Danmarks Elektriske Materialkontroll

<DEMKO> <HAR> 30 10 30
(DEMKO)

National Standards Authority of Ireland

<NSAI> <HAR> 30 30 50
(NSAI)
Norges Elektriske Materiellkontroll
NEMKO <HAR> 10 10 70
(NEMKO)

Asociacion Electrotecnica Y
<UNED> <HAR> 30 10 70
Electronica Espanola (AEE)

Hellenic Organization for

ELOT <HAR> 30 30 70
Standardization (ELOT)

Instituto Portages da Qualidade

np <HAR> 10 10 90
(IPQ)

Schweizerischer Elektro
SEV <HAR> 10 30 90
Technischer Verein (SEV)

Elektriska Inspektoratet SETI <HAR> 10 30 90

Underwriters Laboratories Inc. (UL) SV, SVT, SJ or SJT, 3 X 18AWG

Canadian Standards Association (CSA) SV, SVT, SJ or SJT, 3 X 18AWG

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Shinjuku Monolith, 3-1, Nishi Shinjuku 2-chome, Shinjuku-ku, Tokyo, Japan

Wendenstrasse 14-18, 20097 Hamburg, Germany

3500 Corporate Parkway, Center Valley, Pennsylvania 18034-0610, U.S.A.

491B River Valley Road, #12-01/04 Valley Point Office Tower, Singapore 248373

31 Gilby Road, Mount Waverley, VIC., 3149, Melbourne, Australia

5301 Blue Lagoon Drive, Suite 290 Miami, FL 33126, U.S.A.

M–02
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