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. 2015 Jan 8;517(7533):219-22.
doi: 10.1038/nature14102.

EFF-1-mediated regenerative axonal fusion requires components of the apoptotic pathway

Brent Neumann  1 , Sean Coakley  1 , Rosina Giordano-Santini  1 , Casey Linton  1 , Eui Seung Lee  2 , Akihisa Nakagawa  2 , Ding Xue  2 , Massimo A Hilliard  1
Affiliations

EFF-1-mediated regenerative axonal fusion requires components of the apoptotic pathway

Brent Neumann et al. Nature. .

Abstract

Functional regeneration after nervous system injury requires transected axons to reconnect with their original target tissue. Axonal fusion, a spontaneous regenerative mechanism identified in several species, provides an efficient means of achieving target reconnection as a regrowing axon is able to contact and fuse with its own separated axon fragment, thereby re-establishing the original axonal tract. Here we report a molecular characterization of this process in Caenorhabditis elegans, revealing dynamic changes in the subcellular localization of the EFF-1 fusogen after axotomy, and establishing phosphatidylserine (PS) and the PS receptor (PSR-1) as critical components for axonal fusion. PSR-1 functions cell-autonomously in the regrowing neuron and, instead of acting in its canonical signalling pathway, acts in a parallel phagocytic pathway that includes the transthyretin protein TTR-52, as well as CED-7, NRF-5 and CED-6 (refs 9, 10, 11, 12). We show that TTR-52 binds to PS exposed on the injured axon, and can restore fusion several hours after injury. We propose that PS functions as a 'save-me' signal for the distal fragment, allowing conserved apoptotic cell clearance molecules to function in re-establishing axonal integrity during regeneration of the nervous system.

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