The most widespread chromosomal sex determination systems in plants and animals with separate sexes are the XY system, in which males are heterogametic, and the ZW system, in which females are heterogametic. Figuring out which species has which system has become easier with fully phased, chromosome-scale genome assemblies. A new study now provides such a genome for Amborella trichopoda, with its Z and W sex chromosomes nearly completely assembled.