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Non-viral targeted insertion of large payloads into T cells

Nature Biomedical Engineering volume 8, pages 1516–1517 (2024)Cite this article

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The nuclease Cas9 and DNA-repair pathway homology-mediated end joining can be leveraged to efficiently and non-virally integrate large DNA payloads into genomic target sites in primary T cells.

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Fig. 1: Determination of the optimal window for non-viral DNA transfer in primary T cells.
Fig. 2: Site-specific delivery of large genetic payloads into T cells via Cas9-mediated HMEJ repair.

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  1. Max Delbrück Center for Molecular Medicine in the Helmholtz Society, Berlin, Germany

    Zsuzsanna Izsvák

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  1. Zsuzsanna Izsvák
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Correspondence to Zsuzsanna Izsvák.

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The author declares no competing interests.

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Izsvák, Z. Non-viral targeted insertion of large payloads into T cells. Nat. Biomed. Eng 8, 1516–1517 (2024). https://doi.org/10.1038/s41551-024-01252-0

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