Fosmids
Contents
C. elegans fosmid from the Moerman fosmid library.
Source BioScience LifeSciences is a leading provider of genomic services and products to the life sciences market.
Here is the link for their distribution of Don Moerman's C.elegans fosmid library.
Nomenclature:
| Example | WRM prefix | Plate No. | Filter (a,b,c,d) | Plate Position |
| WRM069bF08 | WRM | 069 | b | F08 |
| WRM069cF08 | WRM | 069 | c | F08 |
| WRM069dF08 | WRM | 069 | d | F08 |
Library info:
| Library Details | C. elegans Fosmid Library |
| Library Plate numbers | 061-069 0610-0641 (41) |
| Number of clones | 12874 (verified) 15744 (total) |
| Plate Format | 384 |
| Source material | nematode total organism |
| Strain | N2 |
| Vector | pCC1FOS (Epicentre) |
| Host | maintained at low copy number until induced. |
| Average insert size | 43.3kB |
| Cloning site | ? |
| Genomic coverage | 5.74X (86.7% correct paired ends) |
| Creator | Don Moerman et al |
| Stock center | SourceBioScience (Formerly:GeneService) |
C. elegans fosmid from the Incyte Genomics Inc. fosmid library.
This is an old fosmid library that was lost for quite some time. 29 of these fosmides were used in the C. elegans sequencing project to fill holes left in the Cosmid assembly.
These clones can be requested from:
Prefered Link: European based copy of the library:HERE
US based copy of the library: Catrina Fronick
Please remember to include a FedEx/DHL Account number to which shipping cost can be charged.
Nomenclature:
| Example | H prefix | Plate No. | Well pos. |
| H27D07 | H | 27 | d07 |
| H30E23 | H | 30 | e23 |
Library info: Easy to Screen High-Density Filters, created 1 November 2000, sold by Incyte Genomics Inc.
| Library Details | Fosmid C. elegans |
| Library Plate numbers | 1-43, 1-5 |
| Number of clones | 16,512 |
| Plate Format | 384 |
| Source material | nematode total organism |
| Strain | N2 |
| Vector | pFOS1 |
| Host | XL1 Blu MR |
| Average insert size | 40 kb |
| Cloning site | BamHI |
| Genomic coverage | 6.6x |
| Creator | Stephanie Chissoe |
| Stock center | G.S.C. St. Louis // The Sanger Institute |
