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. 2024 Nov;92(5):e70016.
doi: 10.1111/aji.70016.

Galectin-1 Elicits a Tissue-Specific Anti-Inflammatory and Anti-Degradative Effect Upon LPS-Induced Response in an Ex Vivo Model of Human Fetal Membranes Modeling an Intraamniotic Inflammation

Jazmin Hernández-Rodríguez  1   2 , Jesús Pérez-Hernández  2 , Pilar Flores-Espinosa  1 , Andrea Olmos-Ortiz  1 , Pilar Velazquez  3 , Rodrigo Zamora-Escudero  4 , Marcela Islas-López  4 , Addy Cecilia Helguera-Repetto  1 , Karla Hernández-Bones  1   5 , Samara Rodríguez-Flores  1   5 , Rodrigo Jiménez-Escutia  1   6 , Amaury Fortanel-Fonseca  7 , Arturo Flores-Pliego  1 , Rosario Lopez-Vancell  2 , Veronica Zaga-Clavellina  1
Affiliations

Galectin-1 Elicits a Tissue-Specific Anti-Inflammatory and Anti-Degradative Effect Upon LPS-Induced Response in an Ex Vivo Model of Human Fetal Membranes Modeling an Intraamniotic Inflammation

Jazmin Hernández-Rodríguez et al. Am J Reprod Immunol. 2024 Nov.

Abstract

Problem: Intrauterine infection is one of the most jeopardizing conditions associated with adverse outcomes, including preterm birth; however, multiple tolerance mechanisms operate at the maternal-fetal interface to avoid the rejection of the fetus. Among the factors that maintain the uterus as an immunoprivileged site, Galectin-1 (Gal-1), an immunomodulatory glycan-binding protein secreted by the maternal-fetal unit, is pivotal in promoting immune cell homeostasis. This work aimed to evaluate the role of Gal-1 during a lipopolysaccharide (LPS)-induced-inflammatory milieu.

Method of study: Using an ex vivo culture with two independent compartments, human fetal membranes at term were pretreated with 40 and 80 ng/mL of Gal-1, then to reproduce an intraamniotic inflammation, the fetal side of membranes was stimulated with 500 ng/mL of LPS for 24 h. The concentrations of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, monocyte chemoattractant protein (MCP1), macrophage inflammatory protein (MIP1) α, regulated upon activation normal T cell expressed and secreted (RANTES), and matrix metalloproteinase (MMP)-9 were measured in both amnion and choriodecidua compartments.

Results: In a tissue-specific fashion profile, pretreatment with the physiologic concentration of Gal-1 significantly diminished the LPS-dependent secretion of TNF-α, IL-1β, Il-6, MCP1, MIP1α, RANTES, and MMP-9.

Conclusion: Gal-1 elicits an anti-inflammatory effect on the human fetal membranes stimulated with LPS, which supports the hypothesis that Gal-1 is part of the immunomodulatory mechanisms intended to stop the harmful effect of inflammation of the maternal-fetal interface.

Keywords: galectin‐1; human chorioamniotic membranes; inflammation; intraamniotic infection; maternal‐fetal unit; preterm birth; preterm labor; tolerogenic.

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