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. 2019 Aug 19:2019:10.17912/micropub.biology.000143.
doi: 10.17912/micropub.biology.000143.

Phase-separated protein dynamics are affected by fluorescent tag choice

Celja Uebel  1 , Carolyn Phillips  1
Affiliations

Phase-separated protein dynamics are affected by fluorescent tag choice

Celja Uebel et al. MicroPubl Biol. .
No abstract available

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Figures

Figure 1.
Figure 1.
Figure 1. PGL-1 fluorescently tagged with mKate2 or mTagBFP2 has distinct phase-separation dynamics compared to PGL-1::GFP. (A) Representative live images of endogenously tagged PGL-1::GFP (top row), PGL-1::mKate2 (middle row), and PGL-1::mTagBFP2 (bottom row) expression in the late pachytene and diplotene region of undissected C. elegans gonads. Scale bar, 10µm. (B) Live images of endogenously tagged PGL-1 in late pachytene zone of buffer dissected gonad. Scale bars, 5µm. (C) Live images of late pachytene region of gonads dissected in 5% 1,6-hexanediol, an aliphatic alcohol. Scale bars, 5µm. (D) Live images of undissected late pachytene region immediately after heat shock of 34°C for 3 hours. Scale bars, 5µm. (E) Live images of pachytene region 5 hours after microinjection of 200µg/mL of the transcriptional inhibitor, α-amanitin. Scale bars, 5µm.
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