Papers by Terd Disayathanoowat
Frontiers in Microbiology, Sep 14, 2023
Diverse microorganisms from the three domains of life (Archaea, Bacteria, and Eukaryota) cause de... more Diverse microorganisms from the three domains of life (Archaea, Bacteria, and Eukaryota) cause deterioration in mural paintings worldwide; however, few studies have simultaneously targeted these three domains. This study aims to survey the microbiome and its potential for biodeterioration on unpreserved Lan Na mural paintings in Sean Khan temple, Chiang Mai, Thailand. The overview of the archaeal, bacterial, and fungal communities was reported by Illumina sequencing, whereas the potential for deterioration was revealed by culturable techniques and a literature search. The abundant microbes reported in this study were also found in other ancient mural paintings worldwide. Halococcus, a salt-tolerant archaeon, as well as the eubacterial genus Crossiella dominated the prokaryotic community. On the other hand, the main fungal group was the genus Candida (Ascomycota). However, a low number of fungi and bacteria were isolated. Most of the isolates showed the ability to survive in the drought conditions of mural paintings but could not perform discoloration activities. The deterioration activity mainly affected calcium compounds, which are the main components of painting substrates. Aspergillus and several bacterial isolates could dissolve calcium compounds, but only Trichaptum species could induce crystal formation. These results suggest that deterioration of painting substrate should be taken into consideration in addition to deterioration of color in mural paintings. For the Lan Na painting in Sean Khan temple, the plaster is the prime target for biodeterioration, and thus we suggest that the preservation effort should focus on this component of the mural painting. KEYWORDS biodeterioration of wall painting, deterioration of cultural heritage, microbiome associated with mural paintings, crystal formation on painting, biomineralization REVIEWED BY
Panuwan Chantawannakul (2 February 1974 – 18 March 2022)
Bee World
Capsid Gene Divergence of Black Queen Cell Virus Isolates in Thailand and Japan Honey Bee Species
Journal of Economic Entomology, May 5, 2015
Black queen cell virus (BQCV) has been found in honey bees worldwide. By using the reverse transc... more Black queen cell virus (BQCV) has been found in honey bees worldwide. By using the reverse transcription polymerase chain reaction (RT-PCR) technique, BQCV was detected in a non-native species, Apis mellifera L., collected in both Thailand and Japan, and three other honey bee species (Apis cerana indica F., Apis dorsata F., and Apis florae F.) native to Thailand and Apis cerana japonica F. native to Japan. Based on the capsid coding region, the phylogenetic analysis showed that the BQCV strains found in A. cerana indica and A. cerana japonica were similar within the group and closer to BQCV in Asia. It is interesting to note that the genetic variation of the BQCV isolates was more associated with geographic origin than the host bee species from which the isolates were obtained.
Isolation and characterization of bacteria from the midgut of the Asian honey bee (<i>Apis cerana indica</i>)
Journal of Apicultural Research, 2012
ABSTRACT Aerobic bacteria were isolated from the midgut of Apis cerana indica on five different m... more ABSTRACT Aerobic bacteria were isolated from the midgut of Apis cerana indica on five different media. The highest count (5.4 x 10(7) colony forming units (CFU/g)) was obtained on Eosin methylene blue (EMB) medium. Fifty-eight isolates were purified and identified using biochemical tests, and 16S rRNA gene sequences were analysed. Two groups of Gamma-proteobacteria and Firmicutes were found. The most abundant species were Klebsiella pneumoniae, Enterobacter cloacae and K. oxytoca. To determine their role in nutrition, enzymes produced from the bacterial isolates were assayed. Protease and lipase activities were detected in some bacterial isolates. The assimilation of fourteen carbon substrates was studied. D-mannose and raffinose showed the highest utilization. Paenibacillus larvae, the cause of American foulbrood disease, was inhibited by some members of Klebsiella.
Proteomics and Immune Response Differences in <i>Apis mellifera</i> and <i>Apis cerana</i> Inoculated with Three <i>Nosema ceranae</i> Isolates
Journal of Proteome Research, May 10, 2023
Applied sciences, Jun 7, 2022
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Insects, Jul 13, 2020
In this study, we examined the impact of Sacbrood virus (SBV), the cause of larval honeybee (Apis... more In this study, we examined the impact of Sacbrood virus (SBV), the cause of larval honeybee (Apis mellifera) death, producing a liquefied a larva sac, on the gut bacterial communities on two larval honeybee species, Apis mellifera and Apis cerana. SBV was added into a worker jelly food mixture and bee larvae were grafted into each of the treatment groups for 24 h before DNA/RNA extraction. Confirmation of SBV infection was achieved using quantitative reverse transcription polymerase chain reaction (RT-qPCR) and visual symptomology. The 16S rDNA was sequenced by Illumina sequencing. The results showed the larvae were infected with SBV. The gut communities of infected A. cerana larvae exhibited a dramatic change compared with A. mellifera. In A. mellifera larvae, the Illumina sequencing revealed the proportion of Gilliamella, Snodgrassella and Fructobacillus was not significantly different, whereas in A. cerana, Gilliamella was significantly decreased (from 35.54% to 2.96%), however, with significant increase in Snodgrassella and Fructobacillus. The possibility of cross-infection should be further investigated.
PLOS ONE, Mar 7, 2019
Bumblebees (tribe Bombini, genus Bombus Latreille) play a pivotal role as pollinators in mountain... more Bumblebees (tribe Bombini, genus Bombus Latreille) play a pivotal role as pollinators in mountain regions for both native plants and for agricultural systems. In our survey of northern Thailand, four species of bumblebees (Bombus (Megabombus) montivagus Smith, B. (Alpigenobombus) breviceps Smith, B. (Orientalibombus) haemorrhoidalis Smith and B. (Melanobombus) eximius Smith), were present in 11 localities in 4 provinces (Chiang Mai, Mae Hong Son, Chiang Rai and Nan). We collected and screened 280 foraging worker bumblebees for microsporidia (Nosema spp.) and trypanosomes (Crithidia spp.). Our study is the first to demonstrate the parasite infection in bumblebees in northern Thailand. We found N. ceranae in B. montivagus (5.35%), B. haemorrhoidalis (4.76%), and B. breviceps (14.28%) and N. bombi in B. montivagus (14.28%), B. haemorrhoidalis (11.64%), and B. breviceps (28.257%).
Gels
This study aimed to develop a film-forming gel containing honey aromatic water (HW) and royal jel... more This study aimed to develop a film-forming gel containing honey aromatic water (HW) and royal jelly (RJ) for cosmetic applications as a facial peel-off mask. HW, which is industrial waste from the water-reduction process of honey, was sterilized by autoclaving and filtration through a 0.22 µm membrane. The film-forming gels were developed using various types of film-forming polymers, including polyvinyl alcohol (PVA 117), carboxymethyl cellulose (CMC), and hydroxyethyl cellulose (HEC). The gel formulations were characterized in terms of their external appearance, viscosity, pH, and drying time, whereas the films generated were characterized by a texture analyzer, microscopic investigation, Fourier transform infrared, and an X-ray diffractometer. The findings highlighted that HW has short storage shelf life due to microbial contamination. Sterilizations were required before further product development. The film-forming gel was created by using the combination of PVA 117, CMC, and HEC...
Microorganisms, Feb 15, 2020
This study investigated both bacterial and fungal communities in corbicular pollen and hive-store... more This study investigated both bacterial and fungal communities in corbicular pollen and hive-stored bee bread of two commercial honey bees, Apis mellifera and Apis cerana, in China. Although both honey bees favor different main floral sources, the dynamics of each microbial community is similar. During pH reduction in hive-stored bee bread, results from conventional culturable methods and next-generation sequencing showed a declining bacterial population but a stable fungal population. Different honey bee species and floral sources might not affect the core microbial community structure but could change the number of bacteria. Corbicular pollen was colonized by the Enterobacteriaceae bacterium (Escherichia-Shiga, Panteoa, Pseudomonas) group; however, the number of bacteria significantly decreased in hive-stored bee bread in less than 72 h. In contrast, Acinetobacter was highly abundant and could utilize protein sources. In terms of the fungal community, the genus Cladosporium remained abundant in both corbicular pollen and hive-stored bee bread. This filamentous fungus might encourage honey bees to reserve pollen by releasing organic acids. Furthermore, several filamentous fungi had the potential to inhibit both commensal/contaminant bacteria and the growth of pathogens. Filamentous fungi, in particular, the genus Cladosporium, could support pollen preservation of both honey bee species.
Insects, Jun 6, 2021
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
FEMS Microbiology Ecology, Nov 14, 2017
The bacterial communities associated with two parasitic mites (Varroa destructor and Tropilaelaps... more The bacterial communities associated with two parasitic mites (Varroa destructor and Tropilaelaps mercedesae) of the honey bee (Apis mellifera) were significantly different.
Diversity
Traditional knowledge of the plants used for textile dyeing is disappearing due to the utilizatio... more Traditional knowledge of the plants used for textile dyeing is disappearing due to the utilization of synthetic dyes. Recently, natural products made from plants have gained global interest. Thus, preserving traditional knowledge of textile dyeing plants is crucial. Here, we documented this knowledge by interviewing 2070 informants from 14 communities of the Chin ethnic group of Myanmar. The Chin communities we interviewed used a total of 32 plant species for textile dyeing from 29 genera in 24 families. Chromolaena odorata, Lithocarpus fenestratus, and L. pachyphyllus were the most important dye species. The most common responses described dyes that were red in color, produced from leaves, derived from tree species, collected from the wild, and used as firewood ash as a mordant to fix the dye to the fabrics. According to the IUCN Red List of threatened species, one species was registered as Data Deficient, 20 species still needed to be categorized, and 11 species were categorized a...
The presence of identical deformed wing virus sequence variants in co-occurring Apis species in Northern Thailand may represent a potential epidemiological threat to native honey bees of Southeast Asia
Journal of Invertebrate Pathology
Animals, Apr 12, 2023
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Microorganisms
Northern Thailand, the main part of the Lanna region, is home to a diverse range of ethnic groups... more Northern Thailand, the main part of the Lanna region, is home to a diverse range of ethnic groups, each with their own food and cultural heritage. The bacterial compositions in fermented soybean (FSB) products indigenous to three Lanna ethnolinguistic groups, including Karen, Lawa, and Shan, were investigated in this study. Bacterial DNA was extracted from the FSB samples and subjected to 16S rRNA gene sequencing using the Illumina sequencing platform. Metagenomic data showed that the predominant bacteria in all FSBs were members of the genus Bacillus (49.5–86.8%), and the Lawa FSB had the greatest bacterial diversity. The presence of genera Ignatzschineria, Yaniella, Atopostipes in the Karen and Lawa FSBs and Proteus in the Shan FSB might be indicators of food hygiene problems during processing. The network analysis predicted antagonistic effects of Bacillus against some indicator and pathogenic bacteria. The functional prediction revealed some potential functional properties of th...
PLOS ONE
Stingless bees play a crucial role in the environment and agriculture as they are effective polli... more Stingless bees play a crucial role in the environment and agriculture as they are effective pollinators. Furthermore, they can produce various products that can be exploited economically, such as propolis and honey. Despite their economic value, the knowledge of microbial community of stingless bees, and their roles on the bees’ health, especially in Thailand, are in its infancy. This study aimed to investigate the composition and the functions of bacterial community associated with Tetragonula pagdeni stingless bees using culture-independent and culture-dependent approaches with emphasis on lactic acid bacteria. The culture-independent results showed that the dominant bacterial phyla were Firmicutes, Proteobacteria and Actinobacteria. The most abundant families were Lactobacillaceae and Halomonadaceae. Functional prediction indicated that the prevalent functions of bacterial communities were chemoheterotrophy and fermentation. In addition, the bacterial community might be able to b...
Life, Feb 3, 2023
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Frontiers in Pharmacology
Background: Cancer is one of the top two leading causes of death worldwide. Ethnobotanical resear... more Background: Cancer is one of the top two leading causes of death worldwide. Ethnobotanical research, it is one of methods, which is able to discover effective anticancer drugs based on “prototype” of indigenous people’s historical experiences and practices. The rhizomes of Paris polyphylla var. yunnanensis (Franch.) Hand.-Mazz. have been used as botanical drugs to treat cancer by Yi, Bai, Dai, and Naxi ethnic groups in Yunnan, China, where this species is widely cultivated in a large scale in Yunnan.Materials and methods: To identify the substances of anticancer activities based on indigenous medicine knowledge, chromatography was performed to separate saponins from the rhizomes of P. polyphylla var. yunnanensis, followed by spectroscopy to determine the structure of six isolated saponins. The cytotoxicity of five extracts and six pure compounds were evaluated by MTS method. Quantitative determination of total saponins of P. polyphylla var. yunnanensis was analyzed by HPLC. Cell cyc...
Journal of Fungi
Opencast limestone mines or limestone quarries are considered challenging ecosystems for soil fun... more Opencast limestone mines or limestone quarries are considered challenging ecosystems for soil fungi as they are highly degraded land with specific conditions, including high temperature, prolonged sunlight exposure, and a lack of organic matter, moisture, and nutrients in soil. In such ecosystems, certain fungi can survive and have a crucial function in maintaining soil ecosystem functions. Unfortunately, we know very little about taxonomic diversity, potential functions, and the ecology of such fungi, especially for a limestone quarry in a tropical region. Here, we characterized and compared the living soil fungal communities in an opencast limestone mine, including mining site and its associated rehabilitation site (9 months post-rehabilitation), with the soil fungal community in a reference forest, using the amplicon sequencing of enrichment culture. Our results showed that living fungal richness in the quarry areas was significantly lower than that in the reference forest, and t...
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Papers by Terd Disayathanoowat