Abstract

Aims: Experiments were designed to investigate the effect of agitation on the production of violacein by a marine bacterium Pseudoalteromonas luteoviolacea.

Methods and Results: A marine sponge‐associated bacterium, P. luteoviolacea, was grown at different agitation speeds. Agitation did not have a significant effect on bacterial growth, but had a profound effect on the size of bacterial aggregate. The production of violacein was the highest under stagnant conditions and decreased with the increase of the agitation speed.

Conclusions: Agitation affected the aggregation of bacterial cells, which, in turn, affected violacein production by P. luteoviolacea.

Significance and Impact of the Study: This study suggests that P. luteoviolacea produced the highest amount of violacein when it was cultured under stagnant conditions.

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