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Does anyone using c. elegans for injections use the Femtojet 4i? I saw discussion regarding Femtojet 4x but the eppendorf manual states that the 4i is optimal for c. elegans. To summarize the manual, FemtoJet 4x: Requires external pressurized air to achieve its higher maximum pressure (up to 10,000 hPa). FemtoJet 4i: Also requires external pressurized air but operates at a lower pressure range (up to 6,000 hPa)

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Hi Maria,

We switched from using the 4x to the 4i. They both have been absolutely fine for microinjections into C. elegans.
You should be ok with the 4i.

Thanks.

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Hi Maria,

We also use the 4i and it works extremely well!

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