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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

TWSG1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
TWSG1 TSG
Sequence Length (AA) Molecular Weight (Da)
223 25017
Protein Name
Twisted gastrulation protein homolog 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MKLHYVAVLT LAILMFLTWL PESLSCNKAL CASDVSKCLI QELCQCRPGE
60 70 80 90 100
GNCSCCKECM LCLGALWDEC CDCVGMCNPR NYSDTPPTSK STVEELHEPI
110 120 130 140 150
PSLFRALTEG DTQLNWNIVS FPVAEELSHH ENLVSFLETV NQPHHQNVSV
160 170 180 190 200
PSNNVHAPYS SDKEHMCTVV YFDDCMSIHQ CKISCESMGA SKYRWFHNAC
210 220 223
CECIGPECID YGSKTVKCMN CMF

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1513 Collapse assay details

Data source: Panorama

Official Gene Symbol
TWSG1
Peptide Modified Sequence
N[+1.0]YSDTPPTSK
Modification Type
Deamidated (N)
Protein - Site of Modification
81
Peptide - Site of Modification
1
Peptide Start
81
Peptide End
90
CPTAC ID
CPTAC-1513
Peptide Molecular Mass
1,109.4877
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tumor tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Accela 1250 Quaternary Low Pump
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-E

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 3.5 4.8 2.4 4.4 4.7 3.3 5.6 6.7 4.1 15 15 15
y5 (1+) 2.5 3 1.7 4.2 3 1.8 4.9 4.2 2.5 15 15 15
y8 (1+) 1.7 2 1.6 2.4 2 2.1 2.9 2.8 2.6 15 15 15
sum 1.2 2 1 2.1 1.8 1.3 2.4 2.7 1.6 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments
').insertAfter(source); //disqus_identifier = identifier+cptac_id; //set the identifier argument //disqus_url = url; //set the url argument //disqus_title = 'Gene: TWSG1, Peptide Sequence: '+identifier+"_"+cptac_id+'- CPTAC Assay Portal'; var disqus_config = function () { this.page.url = url; this.page.identifier = identifier; this.page.title = 'Gene: TWSG1, Peptide Sequence: ' + identifier + '- CPTAC Assay Portal'; }; // Append the Disqus embed script to HTML var dsq = document.createElement('script'); dsq.type = 'text/javascript'; dsq.async = true; dsq.src = '//' + disqus_shortname + '.disqus.com/embed.js'; (document.getElementsByTagName('head')[0] || document.getElementsByTagName('body')[0]).appendChild(dsq); // jQuery('head').append(dsq); // If Disqus exists, call it's reset method with new parameters //DISQUS.reset({ // reload: true, // config: function () { // this.page.identifier = identifier+cptac_id; // this.page.url = url; // this.page.title = 'Gene: TWSG1, Peptide Sequence: '+identifier+"_"+cptac_id+'- CPTAC Assay Portal'; // } //}); } else { // Append the HTML after the target 'source' jQuery('
').insertAfter(source); var comment_array = ["CPTAC-720", "CPTAC-1031", "CPTAC-968", "CPTAC-935", "CPTAC-687", "CPTAC-550", "CPTAC-978", "CPTAC-1724", "CPTAC-1725", "CPTAC-333", "CPTAC-241", "CPTAC-968", "CPTAC-540", "CPTAC-606"]; console.log($.inArray(cptac_id, comment_array)); if ($.inArray(cptac_id, comment_array) != -1) { disqus_identifier = identifier; } else { disqus_identifier = identifier + cptac_id; //set the identifier argument } disqus_url = url; //set the url argument disqus_title = 'Gene: TWSG1, Peptide Sequence: ' + identifier + "_" + cptac_id + '- CPTAC Assay Portal'; // Append the Disqus embed script to HTML var dsq = document.createElement('script'); dsq.type = 'text/javascript'; dsq.async = true; dsq.src = '//' + disqus_shortname + '.disqus.com/embed.js'; (document.getElementsByTagName('head')[0] || document.getElementsByTagName('body')[0]).appendChild(dsq); // jQuery('head').append(dsq); } };