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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

SERPIND1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
SERPIND1 HCF2
Sequence Length (AA) Molecular Weight (Da)
499 57071
Protein Name
Heparin cofactor 2
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MKHSLNALLI FLIITSAWGG SKGPLDQLEK GGETAQSADP QWEQLNNKNL
60 70 80 90 100
SMPLLPADFH KENTVTNDWI PEGEEDDDYL DLEKIFSEDD DYIDIVDSLS
110 120 130 140 150
VSPTDSDVSA GNILQLFHGK SRIQRLNILN AKFAFNLYRV LKDQVNTFDN
160 170 180 190 200
IFIAPVGIST AMGMISLGLK GETHEQVHSI LHFKDFVNAS SKYEITTIHN
210 220 230 240 250
LFRKLTHRLF RRNFGYTLRS VNDLYIQKQF PILLDFKTKV REYYFAEAQI
260 270 280 290 300
ADFSDPAFIS KTNNHIMKLT KGLIKDALEN IDPATQMMIL NCIYFKGSWV
310 320 330 340 350
NKFPVEMTHN HNFRLNEREV VKVSMMQTKG NFLAANDQEL DCDILQLEYV
360 370 380 390 400
GGISMLIVVP HKMSGMKTLE AQLTPRVVER WQKSMTNRTR EVLLPKFKLE
410 420 430 440 450
KNYNLVESLK LMGIRMLFDK NGNMAGISDQ RIAIDLFKHQ GTITVNEEGT
460 470 480 490 499
QATTVTTVGF MPLSTQVRFT VDRPFLFLIY EHRTSCLLFM GRVANPSRS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1508 Collapse assay details

Data source: Panorama

Official Gene Symbol
SERPIND1
Peptide Modified Sequence
DFVN[+1.0]ASSK
Modification Type
Deamidated (N)
Protein - Site of Modification
188
Peptide - Site of Modification
4
Peptide Start
185
Peptide End
192
CPTAC ID
CPTAC-1508
Peptide Molecular Mass
867.3974
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tumor tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Accela 1250 Quaternary Low Pump
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-E

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 13.9 8.4 10.9 17.9 10.2 13.3 22.7 13.2 17.2 15 15 15
y4 (1+) 34.8 25 35.9 33.7 25.4 40.1 48.4 35.6 53.8 15 15 15
y6 (1+) 10.8 5.5 8.8 14.3 4.3 7.2 17.9 7 11.4 15 15 15
sum 9.6 5.8 8.2 11.1 5.4 6.3 14.7 7.9 10.3 15 15 15

Additional Resources and Comments

Resources
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Comments

Assay Details for non-CPTAC-5602 Collapse assay details

Data source: Panorama

Official Gene Symbol
Serpind1
Peptide Sequence
GETHEEVHSVLHFR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
150
Peptide End
163
CPTAC ID
non-CPTAC-5602
Peptide Molecular Mass
1,675.8067
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-E

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (3+) 13 5.4 6.5 14.3 7.6 7.6 19.3 9.3 10 15 15 15
y8 (3+) 18 5.8 8.2 15.8 10.8 8.8 24 12.3 12 15 15 15
y11 (3+) 7.9 5.6 4.1 8 6.7 5.8 11.2 8.7 7.1 15 15 15
y2 (1+) 9.6 6.4 4.4 14.1 8.2 4.2 17.1 10.4 6.1 15 15 15
y3 (1+) 13.6 5 5.4 14.2 11 5.5 19.7 12.1 7.7 15 15 15
sum 4.8 2.6 2 6.7 5.7 2.9 8.2 6.3 3.5 15 15 15

Additional Resources and Comments

Resources
Download SOPs
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Comments

Assay Details for non-CPTAC-5601 Collapse assay details

Data source: Panorama

Official Gene Symbol
Serpind1
Peptide Sequence
TLEAQLTPQVVER
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
347
Peptide End
359
CPTAC ID
non-CPTAC-5601
Peptide Molecular Mass
1,482.8042
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-E

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Loader

Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 17.8 5.4 2.9 30.3 10.1 3.4 35.1 11.5 4.5 15 15 15
b2 (1+) 32.2 8.6 6.1 37.3 7.5 6.8 49.3 11.4 9.1 15 15 15
y6 (1+) 9.8 4.7 4.6 9.4 6.7 4.6 13.6 8.2 6.5 15 15 15
y7 (1+) 19.8 3.4 3.2 15.7 6.4 3.2 25.3 7.2 4.5 15 15 15
y8 (1+) 27 5.5 4.4 20.7 9.7 6.9 34 11.2 8.2 15 15 15
sum 12 2 2.1 13.5 6 3.4 18.1 6.3 4 15 15 15

Additional Resources and Comments

Resources
Download SOPs
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Comments

Assay Details for non-CPTAC-1132 Collapse assay details

Data source: Panorama

Official Gene Symbol
SERPIND1
Peptide Sequence
TLEAQLTPR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
368
Peptide End
376
CPTAC ID
non-CPTAC-1132
Peptide Molecular Mass
1,027.5662
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-E

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 11.1 4.8 3.3 11 5.3 5.1 15.6 7.2 6.1 14 15 15
y3 (1+) 17.2 9.4 5.9 21.4 10.7 22.3 27.5 14.2 23.1 13 15 15
y4 (1+) 7.9 5.3 5.1 12.9 6.4 10 15.1 8.3 11.2 15 15 15
y6 (1+) 10.8 6.5 4.7 9.4 6.7 4.6 14.3 9.3 6.6 15 15 15
y7 (1+) 5.7 6.4 3 11.4 7 3.7 12.7 9.5 4.8 15 15 15
sum 6.2 4.9 2.1 8.3 5.4 3.7 10.4 7.3 4.3 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments
').insertAfter(source); //disqus_identifier = identifier+cptac_id; //set the identifier argument //disqus_url = url; //set the url argument //disqus_title = 'Gene: SERPIND1, Peptide Sequence: '+identifier+"_"+cptac_id+'- CPTAC Assay Portal'; var disqus_config = function () { this.page.url = url; this.page.identifier = identifier; this.page.title = 'Gene: SERPIND1, Peptide Sequence: ' + identifier + '- CPTAC Assay Portal'; }; // Append the Disqus embed script to HTML var dsq = document.createElement('script'); dsq.type = 'text/javascript'; dsq.async = true; dsq.src = '//' + disqus_shortname + '.disqus.com/embed.js'; (document.getElementsByTagName('head')[0] || document.getElementsByTagName('body')[0]).appendChild(dsq); // jQuery('head').append(dsq); // If Disqus exists, call it's reset method with new parameters //DISQUS.reset({ // reload: true, // config: function () { // this.page.identifier = identifier+cptac_id; // this.page.url = url; // this.page.title = 'Gene: SERPIND1, Peptide Sequence: '+identifier+"_"+cptac_id+'- CPTAC Assay Portal'; // } //}); } else { // Append the HTML after the target 'source' jQuery('
').insertAfter(source); var comment_array = ["CPTAC-720", "CPTAC-1031", "CPTAC-968", "CPTAC-935", "CPTAC-687", "CPTAC-550", "CPTAC-978", "CPTAC-1724", "CPTAC-1725", "CPTAC-333", "CPTAC-241", "CPTAC-968", "CPTAC-540", "CPTAC-606"]; console.log($.inArray(cptac_id, comment_array)); if ($.inArray(cptac_id, comment_array) != -1) { disqus_identifier = identifier; } else { disqus_identifier = identifier + cptac_id; //set the identifier argument } disqus_url = url; //set the url argument disqus_title = 'Gene: SERPIND1, Peptide Sequence: ' + identifier + "_" + cptac_id + '- CPTAC Assay Portal'; // Append the Disqus embed script to HTML var dsq = document.createElement('script'); dsq.type = 'text/javascript'; dsq.async = true; dsq.src = '//' + disqus_shortname + '.disqus.com/embed.js'; (document.getElementsByTagName('head')[0] || document.getElementsByTagName('body')[0]).appendChild(dsq); // jQuery('head').append(dsq); } };