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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

FOLH1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
FOLH1 FOLH, NAALAD1, PSM, PSMA
Sequence Length (AA) Molecular Weight (Da)
750 84331
Protein Name
Glutamate carboxypeptidase 2
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MWNLLHETDS AVATARRPRW LCAGALVLAG GFFLLGFLFG WFIKSSNEAT
60 70 80 90 100
NITPKHNMKA FLDELKAENI KKFLYNFTQI PHLAGTEQNF QLAKQIQSQW
110 120 130 140 150
KEFGLDSVEL AHYDVLLSYP NKTHPNYISI INEDGNEIFN TSLFEPPPPG
160 170 180 190 200
YENVSDIVPP FSAFSPQGMP EGDLVYVNYA RTEDFFKLER DMKINCSGKI
210 220 230 240 250
VIARYGKVFR GNKVKNAQLA GAKGVILYSD PADYFAPGVK SYPDGWNLPG
260 270 280 290 300
GGVQRGNILN LNGAGDPLTP GYPANEYAYR RGIAEAVGLP SIPVHPIGYY
310 320 330 340 350
DAQKLLEKMG GSAPPDSSWR GSLKVPYNVG PGFTGNFSTQ KVKMHIHSTN
360 370 380 390 400
EVTRIYNVIG TLRGAVEPDR YVILGGHRDS WVFGGIDPQS GAAVVHEIVR
410 420 430 440 450
SFGTLKKEGW RPRRTILFAS WDAEEFGLLG STEWAEENSR LLQERGVAYI
460 470 480 490 500
NADSSIEGNY TLRVDCTPLM YSLVHNLTKE LKSPDEGFEG KSLYESWTKK
510 520 530 540 550
SPSPEFSGMP RISKLGSGND FEVFFQRLGI ASGRARYTKN WETNKFSGYP
560 570 580 590 600
LYHSVYETYE LVEKFYDPMF KYHLTVAQVR GGMVFELANS IVLPFDCRDY
610 620 630 640 650
AVVLRKYADK IYSISMKHPQ EMKTYSVSFD SLFSAVKNFT EIASKFSERL
660 670 680 690 700
QDFDKSNPIV LRMMNDQLMF LERAFIDPLG LPDRPFYRHV IYAPSSHNKY
710 720 730 740 750
AGESFPGIYD ALFDIESKVD PSKAWGEVKR QIYVAAFTVQ AAAETLSEVA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1491 Collapse assay details

Data source: Panorama

Official Gene Symbol
FOLH1
Peptide Modified Sequence
VPYNVGPGFTGN[+1.0]FSTQK
Modification Type
Deamidated (N)
Protein - Site of Modification
336
Peptide - Site of Modification
12
Peptide Start
325
Peptide End
341
CPTAC ID
CPTAC-1491
Peptide Molecular Mass
1,812.8683
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tumor tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Accela 1250 Quaternary Low Pump
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-E

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 12.6 5.3 8.8 11.5 5.8 10 17.1 7.9 13.3 15 15 15
y5 (1+) 33.6 10.4 9.2 36.4 12.8 10.8 49.5 16.5 14.2 15 15 15
b6 (1+) 10.8 13.3 11.7 14.6 9.8 10.9 18.2 16.5 16 15 15 15
sum 7.3 4.3 5.1 11.3 5.2 7.3 13.5 6.7 8.9 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments
').insertAfter(source); //disqus_identifier = identifier+cptac_id; //set the identifier argument //disqus_url = url; //set the url argument //disqus_title = 'Gene: FOLH1, Peptide Sequence: '+identifier+"_"+cptac_id+'- CPTAC Assay Portal'; var disqus_config = function () { this.page.url = url; this.page.identifier = identifier; this.page.title = 'Gene: FOLH1, Peptide Sequence: ' + identifier + '- CPTAC Assay Portal'; }; // Append the Disqus embed script to HTML var dsq = document.createElement('script'); dsq.type = 'text/javascript'; dsq.async = true; dsq.src = '//' + disqus_shortname + '.disqus.com/embed.js'; (document.getElementsByTagName('head')[0] || document.getElementsByTagName('body')[0]).appendChild(dsq); // jQuery('head').append(dsq); // If Disqus exists, call it's reset method with new parameters //DISQUS.reset({ // reload: true, // config: function () { // this.page.identifier = identifier+cptac_id; // this.page.url = url; // this.page.title = 'Gene: FOLH1, Peptide Sequence: '+identifier+"_"+cptac_id+'- CPTAC Assay Portal'; // } //}); } else { // Append the HTML after the target 'source' jQuery('
').insertAfter(source); var comment_array = ["CPTAC-720", "CPTAC-1031", "CPTAC-968", "CPTAC-935", "CPTAC-687", "CPTAC-550", "CPTAC-978", "CPTAC-1724", "CPTAC-1725", "CPTAC-333", "CPTAC-241", "CPTAC-968", "CPTAC-540", "CPTAC-606"]; console.log($.inArray(cptac_id, comment_array)); if ($.inArray(cptac_id, comment_array) != -1) { disqus_identifier = identifier; } else { disqus_identifier = identifier + cptac_id; //set the identifier argument } disqus_url = url; //set the url argument disqus_title = 'Gene: FOLH1, Peptide Sequence: ' + identifier + "_" + cptac_id + '- CPTAC Assay Portal'; // Append the Disqus embed script to HTML var dsq = document.createElement('script'); dsq.type = 'text/javascript'; dsq.async = true; dsq.src = '//' + disqus_shortname + '.disqus.com/embed.js'; (document.getElementsByTagName('head')[0] || document.getElementsByTagName('body')[0]).appendChild(dsq); // jQuery('head').append(dsq); } };