Hi,
I have got a reference genome which I want to polish with more than just one sequence file.
Basically I've got a reference genome which was generated using PacBio and then I've got 50 whole genome sequences which were generated using Illumina.
Now I want to polish the PacBio reference genome using the "Illumina data". I managed to polish the reference genome once, with just one Illumina data set. But when I go ahead and try to polish the resulting file with the next Illumina data set I get the following error:
[racon::Window::add_layer] error: layer begin and end positions are invalid!

Is there a way to do what I want or is it simply not possible using racon?

I also thought about combining all of the Illumina sequences into one file, but that doesn't seem sensible, regarding I am working on snail genomes each about 1 Gb big....

Thanks in advance!
Laura