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Generation of Chimeras by Aggregation of Embryonic Stem Cells with Diploid or Tetraploid Mouse Embryos

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Transgenic Mouse Methods and Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 693))

Abstract

From the hybrid creatures of the Greek and Egyptian mythologies, the concept of the chimera has evolved and, in modern day biology, refers to an organism comprises of at least two populations of genetically distinct cells. Mouse chimeras have proven an invaluable tool for the generation of genetically modified strains. In addition, chimeras have been extensively used in developmental biology as a powerful tool to analyze the phenotype of specific mutations, to attribute function to gene products and to address the question of cell autonomy versus noncell autonomy of gene function. This chapter describes a simple and economical technique used to generate mouse chimeras by embryo aggregation. Multiple aggregation combinations are described each of which can be tailored to answer particular biological questions.

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Authors and Affiliations

  1. Developmental Biology Program, Sloan-Kettering Institute, New York, NY, USA

    Jérôme Artus & Anna-Katerina Hadjantonakis

Authors
  1. Jérôme Artus
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  2. Anna-Katerina Hadjantonakis
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Correspondence to Anna-Katerina Hadjantonakis .

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Editors and Affiliations

  1. Dept. Pathology & Medical Biology, Medical Biology Section, University Medical Center Groningen, Antonius Deusinglaan 1, Groningen, 9713 AV, Netherlands

    Marten H. Hofker

  2. Mayo Clinic, First Street SW. 200, Rochester, 55905, Minnesota, USA

    Jan van Deursen

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Artus, J., Hadjantonakis, AK. (2011). Generation of Chimeras by Aggregation of Embryonic Stem Cells with Diploid or Tetraploid Mouse Embryos. In: Hofker, M., van Deursen, J. (eds) Transgenic Mouse Methods and Protocols. Methods in Molecular Biology, vol 693. Humana Press. https://doi.org/10.1007/978-1-60761-974-1_3

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  • DOI: https://doi.org/10.1007/978-1-60761-974-1_3

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-973-4

  • Online ISBN: 978-1-60761-974-1

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