DIAMOND is a sequence aligner for protein and translated DNA searches, designed for high performance analysis of big sequence data. The key features are:

• Pairwise alignment of proteins and translated DNA at 100x-10,000x speed of BLAST.
• Frameshift alignments for long read analysis.
• Low resource requirements and suitable for running on standard desktops or laptops.
• Various output formats, including BLAST pairwise, tabular and XML, as well as taxonomic classification.

The online documentation is located at the GitHub Wiki.

Diamond is actively supported and developed software. Please use the issue tracker for malfunctions and the GitHub discussions for questions, comments, feature requests, etc.

DIAMOND is currently developed by Benjamin Buchfink at the Drost lab, Max Planck Institute for Biology, Tübingen, Germany (since 2019). Its development was supported for one year by the German Federal Ministry for Economic Affairs and Energy through an EXIST grant in 2018-2019. It was developed independently by Benjamin Buchfink from 2016-2018. Its initial version was developed in 2013-2015 by Benjamin Buchfink at the Huson lab, University of Tübingen, Germany.

[📧Email] [Twitter] [Google Scholar] [Drost lab] [MPI-BIO]

When using the tool in published research, please cite:

• Buchfink B, Reuter K, Drost HG, "Sensitive protein alignments at tree-of-life scale using DIAMOND", Nature Methods 18, 366–368 (2021). doi:10.1038/s41592-021-01101-x

Further publications:

• Buchfink B, Xie C, Huson DH, "Fast and sensitive protein alignment using DIAMOND", Nature Methods 12, 59-60 (2015). doi:10.1038/nmeth.3176