MYLK

Myosin light chain kinase, smooth muscle also known as kinase-related protein (KRP) or telokin is an enzyme that in humans is encoded by the MYLK gene.

MYLK
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2CQV, 2K0F, 2YR3
Identifiers
Aliases	MYLK, AAT7, KRP, MLCK, MLCK1, MLCK108, MLCK210, MSTP083, MYLK1, smMLCK, myosin light chain kinase, MMIHS, MMIHS1, MYLK-L
External IDs	OMIM: 600922; MGI: 894806; HomoloGene: 14202; GeneCards: MYLK; OMA:MYLK - orthologs
Gene location (Human) Chr. Chromosome 3 (human) Band 3q21.1 Start 123,610,049 bp End 123,884,332 bp
Gene location (Mouse) Chr. Chromosome 16 (mouse) Band 16|16 B3 Start 34,565,580 bp End 34,822,790 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in tail of epididymis saphenous vein seminal vesicula nipple urethra vena cava superficial temporal artery myometrium caput epididymis corpus epididymis Top expressed in iris ascending aorta umbilical cord migratory enteric neural crest cell uterus aortic valve tunica media of zone of aorta ciliary body cervix pineal gland More reference expression data BioGPS More reference expression data
Gene ontology Molecular function transferase activity nucleotide binding protein kinase activity metal ion binding calmodulin binding myosin light chain kinase activity kinase activity protein serine/threonine kinase activity protein binding actin binding ATP binding Cellular component cytoplasm cytosol cell projection stress fiber cleavage furrow cytoskeleton lamellipodium plasma membrane actin cytoskeleton Biological process smooth muscle contraction muscle contraction phosphorylation tonic smooth muscle contraction positive regulation of cell migration positive regulation of wound healing bleb assembly cellular hypotonic response protein phosphorylation aorta smooth muscle tissue morphogenesis positive regulation of calcium ion transport Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 4638 107589 Ensembl ENSG00000065534 ENSMUSG00000022836 UniProt Q15746 Q6PDN3 RefSeq (mRNA) NM_001321309 NM_005965 NM_053025 NM_053026 NM_053027 NM_053028 NM_053029 NM_053030 NM_053031 NM_053032 NM_139300 RefSeq (protein) NP_001308238 NP_444253 NP_444254 NP_444255 NP_444256 NP_444259 NP_444260 NP_647461 NP_001395190 NP_001395191 NP_001395192 Location (UCSC) Chr 3: 123.61 – 123.88 Mb Chr 16: 34.57 – 34.82 Mb PubMed search
Wikidata
View/Edit Human View/Edit Mouse

Function

This gene, a muscle member of the immunoglobulin superfamily, encodes a myosin light-chain kinase, which is a calcium-/calmodulin-dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts.

See also

• Myosin light-chain kinase

Further reading

• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Clayburgh DR, Rosen S, Witkowski ED, et al. (2005). "A Differentiation-dependent Splice Variant of Myosin Light Chain Kinase, MLCK1, Regulates Epithelial Tight Junction Permeability". J. Biol. Chem. 279 (53): 55506–13. doi:10.1074/jbc.M408822200. PMC 1237105. PMID 15507455.
• Wang F, Graham WV, Wang Y, et al. (2005). "Interferon-γ and Tumor Necrosis Factor-α Synergize to Induce Intestinal Epithelial Barrier Dysfunction by Up-Regulating Myosin Light Chain Kinase Expression". Am. J. Pathol. 166 (2): 409–19. doi:10.1016/S0002-9440(10)62264-X. PMC 1237049. PMID 15681825.
• Russo JM, Florian P, Shen L, et al. (2005). "Distinct Temporal-Spatial Roles for Rho Kinase and Myosin Light Chain Kinase in Epithelial Purse-String Wound Closure". Gastroenterology. 128 (4): 987–1001. doi:10.1053/j.gastro.2005.01.004. PMC 1237051. PMID 15825080.
• Shimizu S, Yoshida T, Wakamori M, et al. (2006). "Ca2+–calmodulin-dependent myosin light chain kinase is essential for activation of TRPC5 channels expressed in HEK293 cells". J. Physiol. 570 (Pt 2): 219–35. doi:10.1113/jphysiol.2005.097998. PMC 1464317. PMID 16284075.
• Kim MT, Kim BJ, Lee JH, et al. (2006). "Involvement of calmodulin and myosin light chain kinase in activation of mTRPC5 expressed in HEK cells". Am. J. Physiol., Cell Physiol. 290 (4): C1031–40. doi:10.1152/ajpcell.00602.2004. PMID 16306123.
• Kimura K, Wakamatsu A, Suzuki Y, et al. (2006). "Diversification of transcriptional modulation: Large-scale identification and characterization of putative alternative promoters of human genes". Genome Res. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC 1356129. PMID 16344560.
• Connell LE, Helfman DM (2007). "Myosin light chain kinase plays a role in the regulation of epithelial cell survival". J. Cell Sci. 119 (Pt 11): 2269–81. doi:10.1242/jcs.02926. PMID 16723733.
• Seguchi O, Takashima S, Yamazaki S, et al. (2007). "A cardiac myosin light chain kinase regulates sarcomere assembly in the vertebrate heart". J. Clin. Invest. 117 (10): 2812–24. doi:10.1172/JCI30804. PMC 1978424. PMID 17885681.

External links

• MYLK+protein,+human at the U.S. National Library of Medicine Medical Subject Headings (MeSH)

This article incorporates text from the United States National Library of Medicine, which is in the public domain.