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Causes of the antimicrobial activity of honey

  • Clinical and Epidemiological Studies
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Summary

The present study was performed to clarify the possible causes of the antimicrobial activity of honey. A sugar solution resembling honey in its high sugar content was made. The antimicrobial activities of both honey and this solution towards 21 types of bacteria and two types of fungi were examined. The results achieved by both were compared. The difference between them indicated the presence of antimicrobial substance(s) in honey. The kinds of antimicrobial substances (inhibines) in honey are discussed. Hydrogen peroxide is not the only inhibine in honey. In fact, inhibines in honey include many other substances. Two important classes of these inhibines are the flavonoids and the phenolic acids. Flavonoids have often been extracted from honey previously. In this study two phenolic acids (caffeic acid and ferulic acid) were extracted from honey for the first time.

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References

  1. Dold, D., Du, D. H., Dziao, S. T.: The antibacterial, heat and light sensitive inhibitory substance, inhibine, in natural honey. Z. Hyg. Infektionskrankh. 121 (1937) 333. Quoted from reference 28.

    Google Scholar 

  2. White, J. W., Mary, J. R., Subers, H., Schepartz, A. I.: The identification of inhibine, the antibacterial factor in honey as hydrogen peroxide and its origin in a honey glucose-oxidase system. Biochim. Biophys. Acta 73 (1963) 57–70.

    Article  PubMed  CAS  Google Scholar 

  3. Wakhle, D. M., Desai, D. B.: Estimation of antimicrobial activity of some Indian honeys. Indian Bee J. 53 (1991) 1–4, 80–90.

    Google Scholar 

  4. Molan, P. C.: The antimicrobial activity of honey, 1: The nature of the antibacterial activity. Bee Wld. 73 (1992) 15–28.

    Google Scholar 

  5. McCarthy, J.: The antibacterial effects of honey: medical fact or fiction. Am. Bee J. 135: 5 (1995) 341–342.

    Google Scholar 

  6. Willix, D. J., Molan, P. C., Harfoot, C. G.: A comparison of the sensitivity of wound infecting species of bacteria to the antibacterial activity of manuka honey and other honey. J. Appl. Bacteriol. 73: 5 (1992) 388–394.

    PubMed  CAS  Google Scholar 

  7. Postmes, T., Van-den-Bogaard, A. E., Hazen, M.: Honey for wounds, ulcers and skin graft preservation. Lancet, 341: 8847 (1993) 256–257.

    Article  Google Scholar 

  8. Counting microorganisms. In:Collins, C. H., Lyne, P. M., Grange, J. M. (eds.): Collins and Lyne's microbiological methods. 6th ed. Butterworth, Oxford 1991, pp. 127–140.

    Google Scholar 

  9. National Committee for Clinical Laboratory Standards: Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Tentative standard M7-T2 2nd ed. NCCLS, Villanova, PA 1988. Quoted from: Methods of testing antimicrobial effectiveness. In: Baron, E. J., Finegold, S. M. (eds.): Baily & Scott's diagnostic microbiology, a source book. 8th ed. C. V. Mosby, Baltimore 1990, pp. 171–194.

    Google Scholar 

  10. Krygier, K., Sosulski, F., Hogge, L.: Free, esterified and insolublebound phenolic acids. 1: Extraction and purification procedure. J. Agric. Food Chem. 30 (1982) 330–340.

    Article  CAS  Google Scholar 

  11. Azar, M., Verette, E., Brun, S.: Identification of some phenolic compounds in bilberry juiceVaccinium myrtillus. J Food Sci. 52:5 (1987) 1255–1257.

    Article  CAS  Google Scholar 

  12. Wilkinson, L.: SYSTAT: The system for statistics. SYSTAT. Evanston, IL 1992.

    Google Scholar 

  13. Hamdy, M. H., El-Banby, M. A., Khakifa, K. I., Gad, E. M., Hassanein, E. M.: The antimicrobial effect of honey in the management of septic wounds. Proceedings of Fourth International Conference on Apiculture in Tropical Climates. Cairo, Egypt, 6–10 November 1988. International Bee Research Association, London 1989, pp. 61–67.

    Google Scholar 

  14. Gupta, S. K., Singh, H., Varshney, A. C., Prakash, P.: Therapeutic efficacy of honey in infected wounds in buffaloes. Indian J. Anim. Sci. 62: 6 (1992) 521–523.

    Google Scholar 

  15. Subrahmanyam, M.: Topical application of honey in treatment of burns. Br. J. Surg. 78: 4 (1991) 497–498.

    Article  PubMed  CAS  Google Scholar 

  16. Subrahmanyam, M.: Honey-impregnated gauze versus amniotic membrane in treatment of burns. Burns 20: 4 (1994) 331–333.

    Article  PubMed  CAS  Google Scholar 

  17. Efem, S. E. E., Udoh, K. T., Iwara, C. I.: The antimicrobial spectrum of honey and its clinical significance. Infection 20 (1992) 277–279.

    Article  Google Scholar 

  18. Postmes, T., Van-den-Bogaard, A. E., Hazen, M.: The sterilization of honey with cobalt 60 gamma radiation: a study of honey spiked with spores ofClostridium botulinum andBacillus species. Experientia 51 (1995) 9–10, 986–989.

    Article  Google Scholar 

  19. Sancho, M. T., Muniategui, S., Huidobro, J. F., Simal, J.: Honey from Basque country (Spain). III: Water and sugars. An. Bromatol. 43: 1 (1991) 101–112.

    CAS  Google Scholar 

  20. Huidobro, J. F., Simal, J.: Estimation of sugars in honey. An. Bromatol. 36: 2 (1985) 247–264.

    CAS  Google Scholar 

  21. Chou, T. C., Wu, A. B., Chen, C. Y.: Sugar content of honey from wild and kept honeybees. J. Food Drug Anal. 2: 2 (1994) 97–102.

    CAS  Google Scholar 

  22. Varis, A. L., Helenius, J., Koivulehto, K.: Composition and properties of Finnish honey and their dependence on the season, region, bee race and botanical origin. J. Sci. Agric. Soc. Finland. 55 (1983) 451–463.

    CAS  Google Scholar 

  23. Kim, J. G., Kim, M. K., Lee, S. H.: Study on the content of oligosac-charides in honey from different honey plants. Korean J. Apic. 8: 2 (1993) 165–169.

    Google Scholar 

  24. Poncini, L., Wimmer, F. L.: The composition of some Fiji honeys. Fiji Agric. J. 45: 1 (1983) 25–29.

    CAS  Google Scholar 

  25. Banwart, G. I.: Conditions that influence microbial growth. In:: Basic food microbiology. Abridged textbook ed. AVI, Westport, CT 1980, pp. 73–120.

    Google Scholar 

  26. Harman, A. W.: Hive products for therapeutic use. Am. Bee. J. 39 (1983) 44.

    Google Scholar 

  27. Ribereau-Gayon, P.: Les flavénoides de l'abeille dans le genre. Vitis Compt. Rend. 258 (1964) 1335.

    CAS  Google Scholar 

  28. Rivera-Vargas, L. I., Schmitthenner, A. F., Graham, T. L.: Soybean flavonoids effects on and metabolism byPhytophthora sojae. Phytochemistry 32: 4 (1993) 851–857.

    Article  CAS  Google Scholar 

  29. Geibel, M.: Sensitivity of the fungusCytospora personii to the flavonoids ofPrunus cerasus. Phytochemistry 38: 3 (1995) 599–601.

    Article  CAS  Google Scholar 

  30. Itoh, K., Amamiya, I., Ikeda, S., Konishi, M.: Anti-Helicobacter pylori substances in propolis. Honeybee Sci. 15: 4 (1994) 171–173.

    CAS  Google Scholar 

  31. Ferreres, F., Tomas-Barberan, F. A., Soler, C., Garcia-Viguera, C., Ortiz, A., Tomas-Lorente, F.: A simple extractive technique for honey flavonoid HPLC analysis. Apidologie 25: 1 (1994) 21–30.

    Article  CAS  Google Scholar 

  32. Soler, C., Gil, M. I., Garcia-Viguera, C., Tomas-Barberan, F. A.: Flavonoid patterns of French honeys with different floral origin. Apidologie 26: 1 (1995) 53–60.

    Article  CAS  Google Scholar 

  33. Cizmarik, L., Matel, I.: Examination of the chemical composition of propolis. 1: Isolation and identification of 3,4 dihydroxycinnamic acid (caffeic acid) from propolis. Experientia 26: 4 (1970) 713.

    Article  PubMed  CAS  Google Scholar 

  34. Cizmarik, L., Matel, I.: Examination of the chemical composition of propolis. 2: Isolation and identification of 4-hydroxy-3 methoxycinnamic acid (ferulic acid) from propolis. J. Apic. Res. 12: 1 (1973) 52–54.

    CAS  Google Scholar 

  35. Sosulski, F., Krygier, K., Hogge, L.: Free, estrified and insolublebound phenolic acids. 3: Composition of phenolic acids in cereals and potato flours. J. Agric. Food. Chem. 30 (1982) 337–340.

    Article  CAS  Google Scholar 

  36. Marhuenda Requena, E., Alarcon de la LastrA, C., Garcia Gimenez, M. D.: Mise en évidence des propriétés antibactériennes des acides phénols deThymus carnosus Boiss, isolement des acides caffique, vanillique, p-coumarique, p-hydroxy-benzoique et syringique. Pl. Méd. et Phyt. 21: 2 (1987) 153–159.

    Google Scholar 

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  1. Food Microbiology Laboratory, Central Health Laboratories, Ministry of Health, 19 El Sheik Rihan St., El Falaky, Cairo, Egypt

    H. A. L. Wahdan

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Wahdan, H.A.L. Causes of the antimicrobial activity of honey. Infection 26, 26–31 (1998). https://doi.org/10.1007/BF02768748

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  • DOI: https://doi.org/10.1007/BF02768748

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