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Possible Glial Contribution of Rat Hippocampus Lactate as Assessed with Microdialysis and Stress

  • Conference paper
Clinical Aspects of Microdialysis

Part of the book series: Acta Neurochirurgica Supplement ((NEUROCHIRURGICA,volume 67))

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Summary

Microdialysis for the continuous monitoring of lactate (“lactography”) was applied in rat brain hippocampus in an attempt to establish whether lactate is of neuronal or glial origin. Lactate was analyzed with an electrochemical assay after enzymatic oxidation in dialysates derived from a short-term (1 or 2 days after implantation of the probe) and a long-term (14 or 15 days) preparation. In the short-term experiment the lactate levels in the dialysate were higher without glucose in the perfusate, whereas in the long-term experiment a several fold increase in lactate was observed in the presence of 5 mM glucose. During stress, increases in lactate were virtually similar both in the acute (with or without glucose in the perfusate) and in the chronic preparation (response in the presence of glucose only). In the long term preparation presence of reactive astroglia cells was visualized immunohistochemically with antibodies against glial fibrillary acidic protein. Damage of the hippocampus and the corpus callosum was seen in the chronic preparation with silver impregnation staining. These results emphasize the importance of the presence of glucose in the perfusate and they are consistent with the idea that glial cells contribute to extracellular lactate in the rat hippocampus in vivo and that stress activates the astroglial glycogen pool.

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Abbreviations

GFAP:

glial fibrillary acidic protein

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Author information

Author notes

  1. O. Elekes

    Present address: Pharmacological Research Centre, Chemical Works of GEDEON Richter Ltd., P.O. Box 27, Budapest 10, H-1475, Hungary

Authors and Affiliations

  1. Department of Biological psychiatry, Groningen University, P.O. Box 30.001, NL 9700 RB, Groningen, The Netherlands

    O. Elekes & J. Korf M.D.

  2. Physiologisch-chemisches Institut, Universität Tübingen, Federal Republic of Germany

    K. Venema, F. Postema, R. Dringen & B. Hamprecht

Authors
  1. O. Elekes
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  2. K. Venema
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  3. F. Postema
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  4. R. Dringen
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  5. B. Hamprecht
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  6. J. Korf M.D.
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Editor information

Editors and Affiliations

  1. University Neurosurgical Clinic, Kantonsspital Basel, Basel, Switzerland

    Aminadav Mendelowitsch  & Otmar Gratzl  & 

  2. Department of Research, Kantonsspital Basel, Basel, Switzerland

    Helen Langemann  & Beat Alessandri  & 

  3. Neurosurgical Clinic, Kantonsspital Aarau, Aarau, Switzerland

    Hans Landolt

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© 1996 Springer-Verlag/Wien

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Elekes, O., Venema, K., Postema, F., Dringen, R., Hamprecht, B., Korf, J. (1996). Possible Glial Contribution of Rat Hippocampus Lactate as Assessed with Microdialysis and Stress. In: Mendelowitsch, A., Langemann, H., Alessandri, B., Landolt, H., Gratzl, O. (eds) Clinical Aspects of Microdialysis. Acta Neurochirurgica Supplement, vol 67. Springer, Vienna. https://doi.org/10.1007/978-3-7091-6894-3_1

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  • DOI: https://doi.org/10.1007/978-3-7091-6894-3_1

  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-7091-7426-5

  • Online ISBN: 978-3-7091-6894-3

  • eBook Packages: Springer Book Archive

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