Collections
Drosophila Neurobiology
December, 2024
The fruit fly Drosophila melanogaster has played an important role in uncovering principles of nervous system structure and function, and continues to be a significant model system in neurobiology. This collection represents a comprehensive resource of review articles and protocols in Drosophila neuroscience focusing on neural development, neurophysiology, and behavior. The articles discuss classic approaches as well as newer genetic techniques and behavioral paradigms, and the protocols are designed to be highly accessible for researchers at all levels and with diverse expertise and background.
Mosquitoes
February, 2024
Mosquitoes are important vectors of human and animal pathogens, and significant work is currently being devoted to understanding mosquito ecology and disease transmission mechanisms, and to developing mosquito control methods. This collection includes review articles and a vast selection of experimental procedures for research using mosquitoes, from basic methods for rearing and egg collection, to protocols for genetic manipulation. The collection provides a comprehensive guide for anyone interested in experimental work in this important group of insects.
Mouse Phenotypes
November, 2023
Large-scale mouse mutagenesis projects have resulted in the modification of thousands of mouse genes, and, more recently, CRISPR–Cas gene editing has facilitated the generation of custom mutations. This collection provides advice and practical guidance for gene mutation and analysis in the mouse, starting with the planning stages of gene mutation, through the complete analysis of complex phenotypes.
Experiments in Bacterial Genetics
September, 2023
This collection describes a variety of experimental procedures for genetic research in bacteria. It includes methods for the isolation and propagation of bacteria and phage, DNA purification, and introduction of DNA into bacteria. It also features various genome manipulation strategies, protocols for bacterial genome sequencing and resequencing, and detailed methods for high-throughput mutant screening via transposon sequencing.
Xenopus
September, 2023
This collection encompasses a wide array of experimental procedures for research using Xenopus. It includes basic methods for Xenopus husbandry, egg harvesting, embryo collection, microdissection, and visualization, as well as strategies for extract preparation, genetic manipulation, genomic analysis, transcriptomics, and proteomics.
African Turquoise Killifish
August, 2023
This collection provides a comprehensive selection of protocols covering experimental techniques currently used to study the African turquoise killifish. These protocols should enable studies in the African turquoise killifish and other species of killifish such as the South American killifish, and will be useful to both new and experienced investigators in the field.
Molecular Cloning, 4th edition
Originally published in 2012
Molecular Cloning has served as the foundation of technical expertise in molecular biology laboratories worldwide for several decades. In the fourth edition, originally published in 2012 and now made available as an online article collection, the content was entirely recast to include nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories.
