BIG > Extended Depth of Field
Due to the limited depth of field of brightfield microscopes, it is usually impossible to image large 3-D organisms and objects entirely in focus. By optically sectioning the specimen, however, the in-focus information at the specimen's surface can be acquired over a range of images, which can then be processed to generate a single in-focus image. We have developed different algorithms to achieve
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Saphicon - Software and Hardware for Extended Depth of Field Imaging
Intelligent Software For Photographers - Helicon Soft | Helicon Soft Website
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