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Blood Grouping & Typing Guide

This document describes an experiment to determine blood types through blood grouping and typing. The procedure involves collecting a blood sample from a subject's finger and mixing it with saline solution and antiserum A and B on a glass slide. Agglutination, or clumping, of the blood cells occurs when the antiserum reacts with corresponding antigens in the blood sample. By observing which samples agglutinate, the blood type can be determined as Type A in this case. Saline solution is added to the blood sample to create a suspension for mixing with the antiserums.

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Claveria Jeric Fernandez
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346 views3 pages

Blood Grouping & Typing Guide

This document describes an experiment to determine blood types through blood grouping and typing. The procedure involves collecting a blood sample from a subject's finger and mixing it with saline solution and antiserum A and B on a glass slide. Agglutination, or clumping, of the blood cells occurs when the antiserum reacts with corresponding antigens in the blood sample. By observing which samples agglutinate, the blood type can be determined as Type A in this case. Saline solution is added to the blood sample to create a suspension for mixing with the antiserums.

Uploaded by

Claveria Jeric Fernandez
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as DOCX, PDF, TXT or read online on Scribd

Date: Rating:

Experiment No.6

Blood Grouping and Blood Typing

OBJECTIVE: To learn the technique of groupings and typing fresh and dried blood.

MATERIALS: microscope, glass slides, capillary tubes, saline solution, antiserum A & B,
sterilized needle, fresh human blood, test tube

PROCEDURE:
a. Using sterilized needle, pick the ring finger of subject whose blood group is to be
determined. Collect few drops of blood using capillary tubes.
b. Put 3 ml of saline solution in a test tube and add 2 drops of blood and shake gently.
c. Get a glass slide and mark the left side “A” and the right side “B”.
d. Place 2 drops of the suspended cells in procedure b on “A” and on “B”.
e. Add a drop of anti-A serum on “B” serum on “A”. Rotate the slide in order to mix the
cells and sera.
f. View “A” and “B” mixtures under the low power objectives.

OBSERVATION:
Draw what has been observed under the microscope.
Date: Rating:

CONCLUSION:
1. What happened when the respective serum was added to the red blood cells?

A – the two blood +2 drops of anti-serium

B – the blood agglutinate serum A+2 drops or anti serum

2. What is agglutination?

Agglutinate and occurred when anti-sera reacted w/ their correspondence your


agglutination

3. Why does agglunitination takes place?

Anti-sera reacted w/ serum A

4. What is the group of the fresh blood?

Type A
Date: Rating:

5. Why is saline solution added to the unknown fresh blood specimens?


Infusion of normal saline in high volumes during resuscitation and apheresis can cause
hyperchloremic metabolic acidosis, which may impair the renal function. Normal saline is
also commonly used as an isotonic buffer for red blood cells in research protocols.
6. Fill the table using signs (+ or -) to prove your answer.

SERUM SERUM A
ANTI A ANTI B
A + -
B - +
AB + -
O - +

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